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    20 June 2025, Volume 34 Issue 6
    Differences along an erosion gradient in alpine meadow plant community diversity and factors influencing diversity
    Shuang YAN, Fei XIA, Wei WEI, Jing-long WANG, Hao-yang WU, Lin-ling RAN, Yun-yin XUE, Hao SHI, Shai-kun ZHENG, Jun-qiang WANG, Jun-dong HE
    2025, 34(6):  1-13.  DOI: 10.11686/cyxb2024264
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    This study investigated the plant communities in alpine meadow gullies with differing degrees of erosion and analysed the changes in species diversity and the key factors influencing it in gullies with different erosion levels. We used a quadrat method and randomly selected 1 m×1 m sample plots in mildly, moderately and strongly eroded alpine meadows, sampling alternate erosion gullies, and using no erosion as a control. It was found that the proportion of high-quality forage grasses with Carex moorcroftii as the dominant species gradually decreased with increasing severity of erosion and gradually evolved into a community dominated by poisonous weeds such as Artemisia hedinii. Along the gradient of increasing erosion in the studied alpine meadows, above-ground biomass initially decreased and then increased, and vegetation coverage and species diversity gradually decreased. Compared to the non-eroded sample site, the mildly, moderately, and strongly eroded sample sites showed, respectively, a decrease in above-ground biomass of 38%, 69%, and 16%, a decrease in vegetation coverage of 20%, 46%, and 65%, and a decrease in the Shannon-Wiener index of 11%, 17%, and 76%. A linear negative correlation was observed between the slope gradient and the species diversity within the alpine meadow plant community (P<0.001). Soil erosion leads to changes in soil texture that affect plant growth and plant community diversity. Plant community diversity indices were highly significantly correlated with soil organic matter, particulate organic carbon, pH, and soil sand and clay contents (P<0.01). Shannon-Wiener, Simpson and Margalef indices were all highly significantly negatively correlated with soil pH (P<0.001), while Shannon-Wiener and Simpson indices were both highly significantly positively correlated with soil organic matter (P<0.01). Plant growth indicators were significantly correlated with bulk density (P<0.05). Aboveground biomass was highly significantly and positively correlated with total porosity (P<0.01), but highly significantly and negatively correlated with bulk density of the soil (P<0.001). Vegetation coverage was highly significantly negatively correlated with pH (P<0.01) and sand content (P<0.01), but highly significantly positively correlated with soil organic matter (P<0.001), particulate organic carbon (P<0.01), silt content (P<0.01) and clay content (P<0.01). In summary, with increasing severity of erosion, the vegetation coverage and plant diversity of alpine meadows gradually decreased, and the community structure tended to become more homogeneous. Slope, soil organic matter and pH are the main environmental factors influencing changes in community structure in erosion-affected alpine meadows.

    A study of functional group diversity and productivity of alpine grassland in Qilian Mountain National Park
    Shun-hua LUO, Xin-yu LIU, Bao-ping MENG, Xuan-li CHEN, Ren-jie HU, Hong-yan YU, Xian-ying WANG, Bo ZHANG, Yu QIN
    2025, 34(6):  14-26.  DOI: 10.11686/cyxb2024286
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    Species diversity plays a vital role in maintaining ecosystem function of grasslands and the role of the Qilian Mountains as an ecological barrier. However, our understanding about the relationship between species diversity and ecosystem productivity and the mechanisms involved in maintaining that balance remain limited in this region. Using aerial photography by a lightweight unmanned aerial vehicle and field sampling at seven typical alpine grassland sites in Qilian Mountain National Park in mid-to-late July 2023, we first analyzed the plant community composition of the seven alpine grassland sites, then evaluated the species richness and above-ground biomass of plant functional groups, and finally quantified the influence and mechanism of action of functional group species diversity on productivity. Results show that: 1) The vegetation height in alpine desert was significantly higher than in other grassland types. However, mountain meadow had the highest vegetation cover, species richness and aboveground biomass, with mean values of 91.73%, 16 species and 179.19 g·m-2, respectively. The plant density of alpine swamp meadow was the highest, being up to 4111 plants·m-2. 2) Forbs were found to be the main functional groups in most types of alpine grassland. In mountain meadows, the species richness of forbs was 8, which accounted for about 50% of the total species richness. The sedges contributed more than 90% of the total aboveground biomass in alpine swamp meadow, nevertheless, the sedges gradually decreased and the grasses increased in series represented by alpine meadow, alpine meadow steppe and alpine steppe grasslands. There were only 2-3 functional groups in alpine desert and alpine desert grassland, resulting in few species. Productivity was also low. 3) Species richness of community and functional groups positively correlated with aboveground biomass, indicating that maintaining plant functional group species diversity was conducive to improving ecosystem productivity.

    Effects of partial replacement of chemical nitrogen fertilizers with green manure on soil physical properties and maize (Zea mays) yield
    Wen-li QIN, Jing ZHANG, Guang-min XIAO, Su-qian CUI, Jian-xun YE, Jian-fei ZHI, Li-feng ZHANG, Nan XIE, Wei FENG, Zhen-yu LIU, Xuan PAN, Yun-xia DAI, Zhong-kuan LIU
    2025, 34(6):  27-45.  DOI: 10.11686/cyxb2024399
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    The partial replacement of chemical nitrogen fertilizers with green manure can improve crop yields and soil physical properties. A two-factor split-plot field experiment with maize and hairy vetch was conducted from 2020 to 2022. The main treatment was the cultivation mode before maize planting [winter fallow field (FF) and total return of hairy vetch planted in the winter fallow field (HV)] and the sub-treatment was the nitrogen application level [0 kg·ha-1 (0%N), 135.0 kg·ha-1 (50%N), 270.0 kg·ha-1 (100%N)] to maize as the following crop. The soil physical properties were determined by measuring soil aggregate composition and distribution, mean weight diameter (MWD) and geometric mean diameter (GMD) of aggregates, percentage of aggregate destruction (PAD), soil erodibility factor (K), soil bulk density (BD), total porosity (TP), capillary porosity (CP), non-capillary porosity (NCP), maximum water holding capacity (MWHC), capillary water holding capacity (CWHC), non-capillary water holding capacity (NCWHC), soil organic carbon (SOC) content, and aggregate-associated organic carbon (AOC) content in the 0-10 cm and 10-20 cm soil horizons. Maize yield was also determined. The results showed that, compared with FF, HV significantly increased the proportion of macroaggregates (R0.25) in the 0-10 cm and 10-20 cm soil horizons by 8.95% and 13.13%, MWD by 40.84% and 62.87%, GMD by 30.57% and 51.68%, TP by 5.89% and 5.02%, CP by 1.47% and 0.76%, NCP by 4.42% and 4.25%, MWHC by 15.01% and 13.11%, CWHC by 6.41% and 3.32%, NCWHC by 27.08% and 27.86%, and SOC content by 7.29% and 7.10%, respectively; and significantly reduced PAD by 8.83% and 12.14%, K by 20.79% and 30.73%, and BD by 5.99% and 7.31%, respectively. The AOC content of each particle size fraction in each soil layer and maize yield were significantly higher in HV than in FF. The nitrogen application level had significant or extremely significant impacts on the AOC content of each particle size fraction in each soil layer, R0.25, and other soil physical indexes and maize yield. The following indexes were not significantly different between the FF100%N and HV50%N treatments: SOC content, AOC contents of the >5 mm fraction of the 0-10 cm and 10-20 cm soil horizons, AOC content of the 0.50-1.00 mm fraction of the 0-10 cm soil horizon, the BD, TP, CP, NCP, MWD, CWHC, NCWHC of the 10-20 cm soil horizon, and maize yield. However, the following indexes were higher in the HV50%N treatment than in the FF100%N treatment: AOC contents of the 2.00-5.00 mm, 1.00-2.00 mm, 0.25-0.50 mm, and <0.25 mm fractions of the 0-10 cm and 10-20 cm soil horizon, AOC content of the 0.50-1.00 mm fraction of the 10-20 cm soil horizon, the R0.25, MWD, and GMD of the 0-10 cm and 10-20 cm soil horizons, and the TP, NCP, MWHC, and NCWHC of the 0-10 cm horizon. The PAD and K of the 0-10 cm and 10-20 cm soil horizons and the BD of the 0-10 cm soil horizon were significantly lower in HV50%N than in FF100%N. There were significant or extremely significant correlations between the AOC content of each particle size fraction and SOC content; the content of each particle size fraction and the AOC content; soil physical property indexes and the content of each particle size fraction; and maize yield and soil physical property indexes. The increased SOC content after returning green manure laid the foundation for improved aggregate structure, enhancing soil erosion resistance and WHC. The addition of nitrogen and organic carbon derived from green manure improved the soil’s physical properties. The use of green manure achieved the aims of reducing chemical nitrogen inputs and increasing yield.

    The effect on seed germination and seedling growth of soaking seeds with diethyl aminoethyl hexanoate in alfalfa under NaCl stress
    Can CUI, Meng-qi WANG, Wan-lu ZHAO, Xin-ying LIU, Jing-jing JIAN, Jun-xin YAN
    2025, 34(6):  46-58.  DOI: 10.11686/cyxb2024276
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    The germination process in Medicago sativa seeds is influenced by various factors, among which salt stress is a key limiting factor. There is widespread interest in application of the emerging plant growth promoter diethyl aminoethyl hexanoate (DA-6) in agricultural production, but there is no clear research report on its specific mechanism of action on alfalfa seed germination under salt stress conditions. Consequently, this investigation examined the impact of a 150 mmol·L-1 NaCl environment on the development of alfalfa seeds when subjected to DA-6 concentrations of 2.5, 5.0, 10.0, and 15.0 mmol·L-1. Specifically, our analysis focused on the effects of DA-6 immersion on alfalfa seedlings at both the sprouting and the initial plant formation stages. The findings revealed a substantial decrease in the vitality of M. sativa seeds and a marked suppression of their ability to germinate under conditions of high NaCl concentration. Seed soaking treatment with DA-6 at various concentrations increased the germination rate, germination index, germination potential, root length, stem length, fresh weight and dry weight, and improved the seed germination. The 2.5 mmol·L-1 DA-6 seed soaking treatment increased the activity of antioxidant enzymes in seeds under salt stress. The practice of immersing alfalfa seeds in DA-6 has thus been shown to augment alfalfa seedling resistance to salinity and to stimulate seed sprouting. This enhancement is attributed to an upsurge in the levels of accessible proteins, sugars, and free proline within the seeds. Multivariate analysis showed that the 2.5 and 5.0 mmol·L-1 DA-6 concentrations had the most positive effect on alfalfa seed germination and seedling growth in an otherwise normal environment under NaCl stress.

    Effects of space mutagenesis on the growth of alfalfa (Medicago sativa) seedlings under PEG-6000 simulated drought stress
    Yan-xia ZENG, Zhi-long CHEN, Ji-hong SHANG, Xiao-di SHA, Juan WU, Cai-jin CHEN
    2025, 34(6):  59-69.  DOI: 10.11686/cyxb2024301
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    The aim of this work was to explore the effects of space mutagenesis on the growth of alfalfa (Medicago sativa) seedlings under simulated drought stress. An experiment was conducted in which 14 mutated and 14 non-mutated alfalfa materials were treated with polyethylene glycol 6000 (PEG-6000) to impose drought stress or with distilled water as the control. Growth-related indexes were measured for all the plant materials, and the membership function method was used for multivariate data evaluation. The results showed that the plant height, base stem diameter, root length, fresh weight per plant, leaf area, leaf circumference, leaf length, and leaf width of most materials was lower under drought stress than in the control, whereas the SPAD value was higher in the drought-stressed plants than in the control plants. Compared with the non-mutated materials, the most mutated materials showed lower levels of inhibition under drought stress, and higher SPAD values. With the exception of plant height, the growth indexes of mutated Juneng 7 were significantly higher than those of its non-mutated counterpart (P<0.05). In addition, the base stem diameter and leaf length of mutated WL354, the leaf area, leaf circumference, leaf length, and SPAD value of mutated DS310FY, the leaf area, leaf width, and SPAD value of mutated Yanbao, the leaf area, leaf length, leaf width, and SPAD value of mutated WL343, the leaf length and SPAD value of mutated Gannong No.3, the leaf width of mutated Huangguan, and the SPAD value of mutated Zhongmu No.1, Nanmu 501, and Adina were all significantly higher than those of their respective non-mutated counterparts (P<0.05). The base stem diameter of Adina, fresh weight per plant of Gannong No.6, leaf area, leaf circumference, leaf length of Gannong No.4, and leaf width and SPAD value of WL354 were significantly higher than those of their respective mutated counterparts (P<0.05). We conducted a membership function analysis based on the growth indexes of the materials at the seedling stage under drought stress, and obtained a comprehensive score for each material. The average scores of eight mutated materials (Gannong No.3, DS310FY, Juneng 7, Nanmu 501, Huangguan, Yanbao, WL343 and Adina) were higher than those of their non-mutated counterparts. These results indicate that the drought resistance of these eight materials was improved by space mutagenesis.

    Effects of different pelleted seed coating formulations on seed germination and seedling growth of three brewing sorghum (Sorghum bicolor) cultivars
    Xiao-jing NING, Qiu-jie REN, Li-juan WANG, Xiao-lin JIA, Xiao-jun SHI, Jie WANG
    2025, 34(6):  70-84.  DOI: 10.11686/cyxb2024292
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    Pelletized seed coatings reduce the incidence of pests and diseases, protect seeds against drought stress during direct seeding, and improve the precision of sowing, ensuring healthy seedlings. The aim of this study was to identify the most suitable formulation of pelletized seed coating for three typical varieties of brewing sorghum (Sorghum bicolor) cultivars: Jinnuoliang No. 9, Lunuo No. 8, and Hongyingzi. Different additives including fillers and pelleting aids (gum arabic and diatomite), bioactive substances (the fungicide tebuconazole, the insecticide cyantraniliprole, and the plant growth regulator brassinolide) and functional materials (alginic acid, a super absorbent polymer water retention agent) were combined to create seed coatings. Then, the effects of seed coatings with different formulations on the seed germination and seedling growth of the three sorghum cultivars were investigated in an indoor germination test and a hole tray sowing test. The results showed that a combination of diatomite as the filler and a 15% solution of gum arabic as the bonding agent had a minimal impact on seed germination across the three sorghum varieties when pellets were 5 mm in size. The seed coating agent disintegrated rapidly and there was a high proportion of single-seeded pellets, which contributed to its superior result. The seed coating containing fungicide∶insecticide∶plant growth regulator at a 1∶1∶1 ratio as the bioactive ingredients significantly improved the seed germination rate and the seedling plant height and root length. Specifically, the application of this seed coating ingredient to seeds of Jinnuoliang No. 9, Lunuo No. 8, and Hongyingzi increased the germination rate by 4.6%, 3.1%, and 6.3%, respectively, increased the seedling height by 11.0%, 19.0%, and 38.1%, respectively, and significantly increased the root length by 18.9%, 50.7%, and 100.5%, respectively. Including an appropriate concentration of alginic acid in the seed coating also effectively promoted sorghum seedling growth. Specifically, alginic acid at a concentration 0.1% in the seed coating significantly improved Jinnuoliang No. 9’s seed germination rate by 9.6%, plant height by 32.5%, and root length by 130.9%. Alginic acid at a concentration of 0.3% in the seed coating agent achieved the best results for Lunuo No. 8 and Hongyingzi, significantly increasing their germination rate by 11.8% and 19.7%, plant height by 37.6% and 63.9%, and root length by 113.2% and 156.3%, respectively. The optimal pellet size for sorghum seeds was 5 mm, and the best seed coating formulation was 37.5% diatomite, 9.42% gum arabic, 13.27% alginic acid, 13.27% fungicide, 13.27% insecticide, and 13.27% plant growth regulator. The most effective alginic acid concentration was 0.1% for Jinnuoliang No. 9, and 0.3% for Lunuo No. 8 and Hongyingzi. The results of this research provide scientific data to underpin the development of practical guidelines and technical support for pellet-seeding of brewing sorghum in Southwest China.

    A meta-analysis of fertilizer response of seed yield and seed yield components in Elymus sibiricus
    Yao-bo LIU, Lu PEI, Chen-zhuo LIU, Xiao-xia LI, Bo-kun ZOU
    2025, 34(6):  85-98.  DOI: 10.11686/cyxb2024274
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    The objective of this study was to investigate the fertilizer response of seed yield and seed yield components of Elymus sibiricus under different conditions, in order to provide theoretical guidelines for seed production of E. sibiricus, a native grass species in China. Published research conducted in China was analysed, with unfertilized E. sibiricus serving as the control. By analysing the data from relevant field experiments, the response of seed yield and yield components of E. sibiricus to fertilizer application was quantitatively investigated, along with the effect of stand age, row spacing and regional factors. The results showed that fertilization significantly increased the seed yield of E. sibiricusP<0.001), and had a positive effect on its yield components. The most obvious effects were an overall yield increase from a single application of nitrogen fertilizer and from combined application of N, phosphorus and potassium fertilizers. The effect of fertilizer application on yield of E. sibiricus was better in stands planted for 3-4 years and on E. sibiricus planted with a row spacing of 30 cm, and fertilizer obviously prolonged the production life of E. sibiricus stands. With respect to different climatic and soil conditions, fertilizer application had a better effect on the seed yield of E. sibiricus in semi-humid areas with an average annual precipitation of 400-800 mm, in areas with an average annual temperature of -0.3 to 8 °C, in areas with sandy loam soil, or where soil nutrient imbalances occur through year-round cultivation.

    Response of soil microbial community diversity to patch density of Ligularia virgaurea
    Yu-qin WANG, Mei-ling SONG, Rui ZHOU, Hong-sheng WANG
    2025, 34(6):  99-109.  DOI: 10.11686/cyxb2024280
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    There exist close and complex interactions between soil microorganisms and plants, and it is essential to understand the relationship between plants and soil microbial communities in grassland ecosystems. This study investigated the soil bacterial and fungal populations in Ligularia virgaurea patches of different density. Specifically, patches were identified exhibiting a gradient of six patch densities (D0, D1, D2, D3, D4, and D5). High-throughput sequencing technology was used to analyze the structural changes of bacterial and fungal communities in patches of different density, and to explore the effects of L. virgaurea density on the soil microbial community structure in alpine grasslands. The results showed that the presence of L. virgaurea patches significantly affected the diversity and abundance of soil microbial communities. Proteobacteria was the dominant bacrerial phylum, while Ascomycota, Basidiomycota, and Mortierellomycota were the dominant fungal phyla, and their relative abundances varied significantly among patches. The α-diversity index of soil bacterial communities did not differ significantly between different patches. However, the Chaol and ACE index of the fungal community were significantly higher (P<0.05) in D2 than in D5 patches, and the Shannon and Simpson index were significantly higher in D2 and D5 patches than in D3P<0.05), indicating higher diversity of fungal communities in D2 patches. In summary, the density of L. virgaurea can affect the structure and diversity of soil microbial communities, changing the richness of dominant microbial groups. This result not only reveals the impact of L. virgaurea patches on the species composition and diversity of soil microbial communities, but also enriches our understanding of microbial diversity in alpine grasslands. These data add to the growing body of information available to aid the protection and sustainable utilization of alpine grassland ecosystems.

    Effects of different planting ratios of broomcorn millet (Panicum miliaceum) on ammonia-oxidizing and denitrifying microorganisms in rhizosphere soil of alfalfa (Medicago sativa
    Ruo-xuan LI, Sheng-zhi-can LI, Yi-tong CHEN, Yu-hao SUN, Pei-zhi YANG, Yan-nong CUI, Ming-xiu LONG, Shu-bin HE
    2025, 34(6):  110-121.  DOI: 10.11686/cyxb2024369
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    Companion planting of broomcorn millet (Panicum miliaceum) with alfalfa (Medicago sativa) can improve nitrogen utilization. To explore the mechanism of this effect, we investigated the impact of different planting ratios of broomcorn millet and alfalfa on ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), denitrifying microorganisms (nirK-nirS-, and nosZ-containing microbes), and other soil factors in alfalfa rhizosphere soil under companion planting in the dry farming area of the Loess Plateau. Four broomcorn millet-alfalfa planting ratios [1∶1 (1P1M), 1∶2 (1P2M), 1∶3 (1P3M), and 2∶3 (2P3M)] were established with alfalfa monoculture (M) as the control. The diversity, structure, composition, and co-occurrence network of ammonia-oxidizing and denitrifying microorganisms in alfalfa rhizosphere soil were determined by high-throughput sequencing, and their correlations with soil factors were analyzed. The results showed that, as the ratio of broomcorn millet increased, the soil total nitrogen content and stable carbon and nitrogen isotope values increased, and significantly increased in 2P3M (P<0.05); and the soil organic carbon content first decreased and then increased, with a significant decrease in 2P3M (P<0.05). Companion planting increased the richness of ammonia-oxidizing and nosZ-containing microorganisms, but decreased the richness of nirK-containing microorganisms. The alpha diversity of AOA was sensitive to the planting ratio. However, a beta diversity analysis revealed no significant differences in ammonia-oxidizing and denitrifying community structures among the different planting ratios. Nitrososphaera and Nitrosospira were the dominant genera of AOA and AOB, respectively, accounting for over 85% of their communities based on abundance. The denitrifying microbial community was enriched in genera that promote plant growth and nutrient transformation, such as Achromobacter among the nirK-containing microorganisms and Azoarcus among the nirS-containingmicroorganisms. A co-occurrence network analysis revealed a more complex co-occurrence network of soil ammonia-oxidizing and denitrifying communities in 2P3M than in the other treatments, and this was mainly evident in the network complexity and modularity indicators. The contents of total nitrogen and calcium and magnesium ions were significantly correlated with AOA microbial diversity, and the contents of soil organic carbon and cadmium ions were significantly correlated with AOB and nirS microorganisms. In summary, companion planting affected the diversity, composition, and co-occurrence network of ammonia-oxidizing and denitrifying microorganisms in the rhizosphere soil of alfalfa, revealing the microbial mechanism of efficient nitrogen utilization under this technology. Based on these results, the broomcorn millet∶alfalfa planting ratio of 2∶3 appears to be the most effective for exploiting the advantages of companion planting.

    Effects of supplemental feeding on growth rate, and serum biochemical and metabolomic parameters of grazing Tan sheep
    Qian XU, Shuai GUO, Liang-liang ZHANG, Zhan-jun WANG, Guo-sheng XIN
    2025, 34(6):  122-138.  DOI: 10.11686/cyxb2024289
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    The aim of this experiment was to investigate the effects of supplemental feeding on growth rate, blood biochemistry, and antioxidant and blood metabolomic parameters of grazing Tan sheep. In the experiment, twenty-four 3-month-old castrated male Tan sheep, with similar body weight (23.38±0.70 kg), were randomly divided into four groups: a ‘no concentrate’ group (NC, control) which were grazed only, and low energy (LE), medium energy (ME), and high energy (HE) groups which were the experimental groups and received supplemental concentrate feed before grazing. The digestible energy content of supplemented concentrate was 15.91, 17.63 and 18.17 MJ·kg-1 for LE, ME and HE, respectively. There was a pre-trial adjustment period lasting 12 days and the experimental period lasted for 90 days. The results show: 1) Compared with NC and LE, the final weight and daily weight gain of the HE and ME groups was significantly increased (P<0.05). 2) The economic return of ME was higher than the economic returns from the other groups. 3) Fasting blood glucose, and levels of total cholesterol, triglycerides, creatinine, aspartate aminotransferase and low-density lipoprotein cholesterol concentrations in the blood of the sheep all tended to increase with increase in supplemental concentrate energy level, with triglyceride content in HE being notably higher than in the other groups (P<0.05). 4) The energy level markedly affected the animals’ antioxidant capacity; the serum malondialdehyde content in HE was significantly higher than in NC and LE (P<0.05). In addition, the total antioxidant capacity in ME was notably higher than the NC (P<0.05). Moreover, glutathione peroxidase activity in NC was notably higher than in LE and ME (P<0.05). 5) The plasma metabolomics analysis identified a total of 17 metabolites with significant differences. The enriched metabolic pathways were mainly purine metabolism, nucleotide metabolism, primary bile acid biosynthesis, arachidonic acid metabolism, cholesterol metabolism, and tyrosine metabolism. In summary, increasing energy level effectively improved the growth rate of grazing Tan sheep. Blood biochemistry and metabolomic data indicate this improvement may be through the regulation of purine metabolism, arachidonic acid metabolism and other pathways and their intermediate metabolites to enhance body antioxidant and immune functions. Under the conditions of this experiment, the supplemental concentrate level of 0.45 kg·animal-1·d-1 (ME) showed the best effect.

    Cloning of the MtBMI1 gene from Medicago truncatula and its role in drought tolerance
    Yuan-yuan ZHAO, Xiao-jian PU, Cheng-ti XU, Wei WANG, Yun-jie FU
    2025, 34(6):  139-153.  DOI: 10.11686/cyxb2024303
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    Drought significantly impedes the normal growth, development, and reproduction of plants, and has emerged as a pivotal factor contributing to the decline in crop and forage yields. The BMI1 protein, a key component of the Polycomb Group (PcG) protein complex that mediates histone ubiquitination, is crucial for the epigenetic regulation of plant responses to abiotic stresses. This study reports the cloning of MtBMI1, which encodes a member of the PcG family, from Medicago truncatula. Our analyses showed that the MtBMI1 gene sequence spans 5386 base pairs and encodes a protein comprising 429 amino acids, featuring two functional domains: zf-C3HC4 and RAWUL. Phylogenetic analyses revealed close relationships between MtBMI1 and PvBMI1-1 of Phaseolus vulgaris and GmBMI1-1 of Glycine max. A subcellular localization analysis in tobacco epidermal cells confirmed the nuclear localization of the MtBMI1 protein. Moreover, GUS staining analyses revealed robust activity of the MtBMI1 promoter in the mature inflorescence, stigma, flower, stem, and pedicel of Arabidopsis. Functional analysis via overexpression in Arabidopsis demonstrated that the histone H2AK119ub content was significantly higher (P<0.05) in transgenic lines than in wild type. Under drought stress, transgenic lines exhibited pronounced water-loss phenotypes, with reduced root length, root fresh weight, and aboveground fresh weight (P<0.05), and elevated levels of malondialdehyde (P<0.05), compared with the wild type. These findings suggest that MtBMI1 exerts a negative regulatory effect on M. truncatula’s response to drought stress. The results of this study offer insights into the epigenetic mechanisms underlying drought stress tolerance in this model legume species.

    Expression analysis of AP2/ERFs genes in alfalfa regulated by exogenous NO under drought stress
    Xiao-Yue WEN, Ying ZHAO, Bao-qiang WANG, Xian WANG, Xiao-lin ZHU, Yi-zhen WANG, Xiao-hong WEI
    2025, 34(6):  154-167.  DOI: 10.11686/cyxb2024277
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    Alfalfa (Medicago sativa) is the most widely grown forage legume crop in the world. APETALA2/ethylene-responsive (AP2/ERF) transcription factors play a key role in plant resistance to abiotic stress. Nitric oxide (NO), as a signaling molecule in plants, plays an important role in plant drought resistance. In this study, bioinformatics methods were used to identify members of the alfalfa MsAP2/ERF gene family and analyze their response patterns to NO and drought. The MsERF07 gene, which strongly responds to NO regulation, was screened from the MsAP2/ERF gene family for subcellular localization. The results showed that all members of the family contained AP2 domains, and the number of amino acids in their proteins ranged from 176 to 422. Subcellular localization predicted that most proteins were localized in the nucleus. MsERF01 and MsERF11 were closely related and had similar domains. 61.54% of MsAP2/ERF genes contained only exons and also had highly similar conserved motifs. Protein interaction showed that MsERF01 and MsERF11, MsERF05 and MsERF07 were all located at the same node in the protein interaction map. The 13 MsAP2/ERF gene family members were unevenly distributed on 13 chromosomes, and there were 43 cis-regulatory elements related to light response, tissue-specific expression, stress and plant hormones in the promoter sequences of the MsAP2/ERF gene family members. In addition, transcriptome sequencing data analysis of alfalfa showed that the expression of most MsAP2/ERF gene family members increased under the regulation of NO, and further qRT-RCR experimental results showed that exogenous NO promoted the expression of MsAP2/ERF genes under drought stress. The MsERF07 gene was cloned, and subcellular localization results showed that the protein was localized in the nucleus and cell membrane. This study provides a basis for subsequent research on the molecular mechanism of the alfalfa MsERF07 gene action in response to drought stress.

    A study of salt tolerance differences in Cyperus esculentus at different growth stages in a sandy saline soil
    Qing-qing ZHANG, Xing-yu MA, Yan LU, Guang-Xing ZHAO, Fan-jiang ZENG, Cai-bian HUANG
    2025, 34(6):  168-180.  DOI: 10.11686/cyxb2024287
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    This research investigated the effects of different NaCl concentrations on the growth and physiology of Cyperus esculentus at different growth stages, and comprised a pot experiment with a gradient of five different NaCl stress levels: 0 g·kg-1 (control), 0.5 g·kg-1 (mild), 1.0 g·kg-1 (moderate), 2.0 g·kg-1 (severe), and 3.5 g·kg-1 (saline soil) of added NaCl. The biomass accumulation, levels of osmotic adjustment substances and antioxidant enzyme activities of C. esculentus were then analyzed at the seedling stage and different tuber development stages, and salt tolerance in the different stages evaluated. It was found that mild salt stress seriously inhibited the aboveground and underground growth of C. esculentus at the seedling stage, and the biomass did not change significantly after salt stress. From the early stage to the late stage of tuber maturity, the aboveground and underground biomass was significantly lower with increase in salt stress, and growth ceased during the middle stage of tuber development in the severe and saline treatments. The contents of Na+ and Cl- increased significantly with increase in salt stress, while the K+∶Na+ ratio decreased. C. esculentus adapted to salt stress mainly through the accumulation of soluble protein, soluble sugar and betaine, and the increase of catalase (CAT) and superoxide dismutase (SOD) activity during the seedling stage and early tuber development. In the middle of the tuber development stage, a significant increase in SOD activity was the primary mechanism for alleviation of salt stress. In the late tuber stage, the acclimation to salt stress was through the accumulation of soluble protein and betaine and increased SOD activity. The salt tolerance of C. esculentus at the different growth stages ranked seedling stage>late tuber stage>middle tuber stage>early tuber stage. From a perspective of a multivariate analysis of plant traits, soil NaCl concentration≤1.0 g·kg-1 is recommended. The results of this study provide scientific data to aid development of practical guidelines for the large-scale planting of C. esculentus in sandy salinized land in Xinjiang Province.

    Effect of rotational tillage patterns on the crop yield and quality in a maize-wheat (Zea mays-Triticum aestivum) double cropping system in dryland agriculture
    Pei-pei JIANG, Jin-hua GUO, Hui-shu XIAO, Yan-min PENG, Jun ZHANG, Wen-zhong TIAN, Jun-jie Lyu, Jin-zhi WU, He-zheng WANG, Guo-zhan FU, Ming HUANG, You-jun LI
    2025, 34(6):  181-192.  DOI: 10.11686/cyxb2024297
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    The objectives of this study were to investigate the effect of different rotational tillage patterns (i.e. rotation of tillage and no-tillage cropping in different years) on crop yield and quality in a dryland summer maize (Zea mays)-winter wheat (Triticum aestivum) double cropping system (hereafter referred to as the maize-wheat double cropping system). A field experiment was conducted with five treatments: no tillage in both the maize and wheat seasons (MNWN), subsoiling in the maize season and no tillage in the wheat season (MSWN), no tillage in the maize season plus 3 consecutive years of no-tillage and 1 year of ploughing in the wheat season (MNW3N1P), subsoiling in maize season plus 3 consecutive years of no-tillage and 1 year of ploughing in the wheat season (MSW3N1P), and conventional consecutive ploughing in the maize and wheat seasons (CK). These treatments were established in 2004 at the Luoyang Arid Farming Experimental Station of the Chinese Academy of Agricultural Sciences. The crop yields in 2015-2021, the grain nitrogen, phosphorus, and potassium contents, the protein yield of maize and wheat, and the contents of grain protein components of wheat in 2020-2021 were determined. The results showed that the differences in the grain yields of summer maize and winter wheat were not significant between MNWN and the rotational tillage treatments (MSWN, MNW3N1P, and MSW3N1P). However, the grain yields of summer maize and winter wheat were higher in all of these patterns than in CK, and the extent of the improvement varied depending on the annual precipitation. The use of rotational tillage resulted in a considerable yield enhancement compared with CK, with increases of 56.2%-65.2% for wheat (increases of 28.2%-32.6% for annual yield in the dry year) and 47.5%-57.7% for maize (increases of 25.7%-29.8% for annual yield in a normal year), and 18.9%-31.2% for wheat yield in a rainy year. Among the three rotational tillage patterns, MSW3N1P showed the best effect in terms of enhancing the grain nitrogen, phosphorus, and potassium contents and the protein yield for maize and wheat, and to improve the contents of grain protein components in wheat. Compared with CK, MSW3N1P resulted in significantly increased nitrogen and potassium contents and protein yield in maize grains (by 4.5%, 15.6%, and 25.8%, respectively), and increased nitrogen and phosphorus contents, protein yield, gluten content, glutenin∶gliadin ratio, and storage protein content in winter wheat grains (by 7.7%, 15.2%, 109.8%, 16.1%, 9.6%, and 11.9%, respectively), ultimately leading to a 38.5% increase in annual protein yield. While the MNW3N1P treatment also significantly increased wheat grain gluten content, the glutenin∶gliadin ratio, and storage protein content (by 15.9%, 14.4%, and 9.2%, respectively, compared with CK), the MSW3N1P treatment further significantly increased the gliadin and storage protein contents in wheat grain by 5.4% and 2.5%, respectively, compared with MNW3N1P. Therefore, MSW3N1P is recommended as the optimal rotational tillage pattern for high-yield and high-quality crops in dryland maize-wheat double cropping systems.

    Effect of nitrogen and phosphorus combined application on forage yield of Elymus sibiricus in an alpine region
    Qi-lin LIU, Xiao-jun WANG, Jin-lan WANG, Wen-hui LIU, Qiao-ling MA, Jian-hui LI, Sheng-yuan ZHANG, Wen-xia CAO, Wen LI
    2025, 34(6):  193-202.  DOI: 10.11686/cyxb2023459
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    This research investigated the effects of different application rates of nitrogen and phosphorus fertilizer on forage yield of Elymus sibiricus cv. Qingmu No. 2 in an alpine region. Four nitrogen fertilizer rates (0, 60, 90, 120 kg N·ha-1) and four phosphate fertilizer rates (0, 30, 60, 90 kg P·ha-1) levels were included in the experiment to identify the effect of the various N and P fertilizer combinations on E. sibiricus growth traits, forage yield and economic return. Structural equation modelling was used to assess the pathways by which effects of the different N and P combinations influenced the forage yield of E. sibiricus. This research provides data to underpin extension advice to farmers on how to achieve efficient production of high-quality forage in alpine regions. It was found: 1) Plant height, stem diameter, tiller numbers, fertile tiller numbers, forage yield and economic return of E. sibiricus were significantly increased by combined N and P application, and those traits showed a pattern of initial increase and then decrease with increase in P application at the same N application level. 2) The forage yield and net income were the highest (12154.14 kg·ha-1 and 3370.49 CNY·ha-1 respectively), in the treatment with a combined application of 60 kg N·ha-1 and 60 kg P·ha-1. 3) The structural equation model showed that tillering is the primary driving factor for improving the forage yield of E. sibiricus under combined application of N and P fertilizer. 4) Integrated evaluation of traits using a TOPSIS model showed that the combined application of 60 kg N·ha-1 and 60 kg P·ha-1 not only maintained higher forage yield, but also significantly improved economic returns, compared to other treatments. This N and P combination is therefore recommended as suitable management practice for forage production of E. sibiricus in alpine regions.

    Effects of jujube tree on the growth of alfalfa and orchardgrass in different positions within an orchard
    Ying-hao ZHANG, Chu-bo LIU, Kun ZHOU, Jia-cun GUO, Shi-peng LIU, Luan-zi SUN
    2025, 34(6):  203-212.  DOI: 10.11686/cyxb2024270
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    Enhancing interspecific facilitation and reducing interspecific competition are key to achieving ecological and high-quality development in fruit-grass production systems. Fruit trees have stronger competitiveness than grasses, but the impact of fruit trees on the growth of nearby grasses in in orchards and the main limiting factors are still unclear. This study was conducted in Yanchuan, a major jujube (Ziziphus jujube) production area in the Yellow River floodplain of northern Shaanxi. The jujube orchard had a full ground cover of alfalfa (Medicago sativa) and orchardgrass (Dactylis glomerata), and the biomass of the herbage photosynthetically active radiation, soil nutrients, and water content were measured in different compass directions and at various distances from the jujube trees. Spatial differences were found in the growth of grass in the orchard, with the highest biomass of grass located to the south of the trees (alfalfa 22.57 g·m-2, orchardgrass 174.57 g·m-2), followed by the east and west, and the lowest to the north (alfalfa 14.29 g·m-2, orchardgrass 71.84 g·m-2). Meanwhile, the closer the proximity to the jujube tree, the more the growth of grass was inhibited. In different compass directions from the jujube tree, there were significant differences in photosynthetically active radiation (PAR) intercepted by the alfalfa and the orchardgrass, with the highest PAR to the south and the lowest to the north of the trees. In all directions, the soil nitrogen and phosphorus nutrient content increased with closer proximity to the tree, while the soil moisture content and photosynthetically active radiation decreased, and the root-shoot ratio of alfalfa and orchardgrass tended to increase. These results reveal that the main factor causing the difference in the growth of alfalfa and orchardgrass in different directions from the jujube trees is competition for light. As the distance of alfalfa and orchardgrass from the trees decreased, the main competitive factor between them was water, followed by light. There was no obvious competition in terms of soil nutrients.

    Effects of biological and antifungal additives on ensiling characteristics, in vitro digestibility, gas production, and aerobic stability of fermented total mixed ration including wet brewers’ grains
    Si-ran WANG, Cheng-long DING, Ji-peng TIAN, Yun-hui CHENG, Neng-xiang XU, Wen-jie ZHANG, Xin WANG, Bei-yi LIU
    2025, 34(6):  213-226.  DOI: 10.11686/cyxb2024295
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    Wet brewers’ grain (WBG) is an industrial by-product that is produced in large quantities, and is rich in nutrients, including crude proteins, vitamins, and minerals. However, the high water content makes it challenging to preserve WBG, and this severely limits its use in ruminant farming. Hence, the aim of this study was to investigate the effects of biological and antifungal additives on the fermentation quality, in vitro digestibility, and aerobic stability of fermented total mixed ration (TMR) containing WBG at a concentration of 100 g·kg-1 fresh weight (FW). The TMR was ensiled in 20-L silos with six treatments: 1) no additives (Control); 2) Lacticaseibacillus casei (LC; applied at 1×106 cfu·g-1 FW); 3) Lentilactobacillus buchneri (LB; 1×106 cfu·g-1 FW); 4) potassium sorbate (POS; 1 g·kg-1 FW); 5) sodium diacetate (SOD; 5 g·kg-1 FW); and 6) calcium propionate (CAP; 5 g·kg-1 FW). All silos were opened after 100 days of ensiling to evaluate the fermentation quality and in vitro digestibility of the silages, followed by a 14-day aerobic stability test. All the TMR silages were well-preserved with low pH (4.32-4.47) and acceptable levels of butyric acid (0.837-1.750 g·kg-1 dry matter) and ammonia nitrogen (76.7-91.8 g·kg-1 total nitrogen). The control, LB, POS, and SOD silages remained stable during the 14-day aerobic stability test, and the POS silage was the most stable. Compared with the other silages, the POS silage had smaller populations of aerobic bacteria (5.50 vs. 6.56-7.66 log10 cfu·g-1 FW) and mold (4.93 vs. 4.96-5.91 log10 cfu·g-1 FW) on day 14 of the aerobic stability test. The POS and CAP silages showed increased in vitro digestibility of dry matter (62.5%-65.4% vs. 55.3% in the control) and neutral detergent fiber (56.3%-59.1% vs. 48.2% in the control). Overall, POS (applied at 1 g·kg-1 FW) is recommended as an additive to improve the fermentation quality, aerobic stability, and in vitro digestibility of fermented TMR containing 100 g·kg-1 FW WBG.

    Effects of different mixture ratios and lactic acid bacteria on the quality and aerobic stability of mixed silage made from whole maize (Zea mays) and soybean (Glycine max) plants
    Zong-yang KUANG, Lin MU, Lan WEI, Yang GUO, Gui XU, Yao CHEN, Xue-yun SHI, Zhong-shan WEI, Zhi-fei ZHANG
    2025, 34(6):  227-238.  DOI: 10.11686/cyxb2024347
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    Maize (Zea mays) and soybean (Glycine max) are often grown with a strip intercropping cultivation mode in southern China. The aim of this study was to explore the effects of different mixture ratios and the addition of lactic acid bacteria on the quality and aerobic stability of mixed silage made from whole maize and soybean plants. Whole plants of maize and soybean were harvested separately, crushed, and mixed at various proportions, and then additives were added before ensilage. A two-factor completely randomized experiment was conducted. Factor A was the ratio of maize to soybean (based on mass, in kg), with the following treatments 1∶0 (A1), 2∶1 (A2), 1∶1 (A3), 1∶2 (A4), and 0∶1 (A5). Factor B was the type of lactic acid bacteria added, with the following treatments: Lactobacillus buchneri (LB), Lactobacillus plantarum (LP), and no Lactobacillus (L0). After 60 days of fermentation, the nutritional quality, fermentation quality, and aerobic stability of the silages were analyzed. The results showed that the effect of the interaction between the mixing ratio and the addition of lactic acid bacteria was extremely significant (P<0.01) for the contents of crude protein, neutral detergent fiber, acid detergent lignin, ether extract, and crude ash, the pH value, the contents of lactic acid, acetic acid, and propionic acid, the ammonia nitrogen/total nitrogen ratio (NH3-N/TN), and the duration of aerobic stability of the maize-soybean mixed silages. The contents of neutral detergent fiber, acid detergent fiber, and lactic acid were significantly higher in the A2 and A3 mixed treatment groups than in the other three mixed treatment groups (P<0.05). Compared with the L0 group, all treatments in the LP group, except for the A4 mixed treatment group, had significantly higher lactic acid contents (P<0.05) and significantly lower crude ash contents (P<0.05). Except for the A5 mixed treatment group, the other mixed treatment groups in the LP group had significantly lower pH values than those of the L0 group (P<0.05). Compared with the L0 group, all the mixed treatment groups in the LB group had significantly lower acid detergent lignin contents (P<0.05). The acetic acid content was significantly higher (P<0.05) in the A1, A2, and A3 mixed treatment groups than in the other groups, and the duration of aerobic stability was also longer. A grey relational analysis was conducted based on the 11 core indicators of each mixed silage treatment group. Among all the treatment groups, the top three in terms of a good ensilage effect as indicated by the weighted relational degrees were A2LP, A3LB, and A1LP. In conclusion, when making silage from whole maize and soybean, the mass proportion of soybean should be less than or equal to that of maize, and L. plantarum should be added to improve the fermentation quality.