Response of soil microbial community diversity to patch density of Ligularia virgaurea

doi:10.11686/cyxb2024280

Abstract

Abstract:

There exist close and complex interactions between soil microorganisms and plants， and it is essential to understand the relationship between plants and soil microbial communities in grassland ecosystems. This study investigated the soil bacterial and fungal populations in Ligularia virgaurea patches of different density. Specifically， patches were identified exhibiting a gradient of six patch densities （D₀， D₁， D₂， D₃， D₄， and D₅）. High-throughput sequencing technology was used to analyze the structural changes of bacterial and fungal communities in patches of different density， and to explore the effects of L. virgaurea density on the soil microbial community structure in alpine grasslands. The results showed that the presence of L. virgaurea patches significantly affected the diversity and abundance of soil microbial communities. Proteobacteria was the dominant bacrerial phylum， while Ascomycota， Basidiomycota， and Mortierellomycota were the dominant fungal phyla， and their relative abundances varied significantly among patches. The α-diversity index of soil bacterial communities did not differ significantly between different patches. However， the Chaol and ACE index of the fungal community were significantly higher （P<0.05） in D₂ than in D₅ patches， and the Shannon and Simpson index were significantly higher in D₂ and D₅ patches than in D₃ （P<0.05）， indicating higher diversity of fungal communities in D₂ patches. In summary， the density of L. virgaurea can affect the structure and diversity of soil microbial communities， changing the richness of dominant microbial groups. This result not only reveals the impact of L. virgaurea patches on the species composition and diversity of soil microbial communities， but also enriches our understanding of microbial diversity in alpine grasslands. These data add to the growing body of information available to aid the protection and sustainable utilization of alpine grassland ecosystems.

Key words: Ligularia virgaurea, density, soil microorganism, community composition

Yu-qin WANG, Mei-ling SONG, Rui ZHOU, Hong-sheng WANG. Response of soil microbial community diversity to patch density of Ligularia virgaurea[J]. Acta Prataculturae Sinica, 2025, 34(6): 99-109.

Figures/Tables 7

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斑块Patch	物种数Number of species	主要优势植物（重要值） The main dominant plants （importance value）
D₀	22.25±0.75a	高原早熟禾P. alpigena （13.65%）、矮嵩草 C. alatauensis （12.81%）、线叶嵩草C. capillifolia（7.96%）、钝裂银莲花A. obtusiloba （4.95%）
D₁	24.25±1.25a	高原早熟禾P. alpigena （10.85%）、矮嵩草 C. alatauensis （9.96%）、黄帚橐吾L. virgaurea（8.74%）、线叶嵩草 C. capillifolia （7.97%）
D₂	23.50±1.50a	高原早熟禾P. alpigena （13.74%）、矮嵩草 C. alatauensis （10.60%）、黄帚橐吾L. virgaurea（9.47%）、线叶嵩草 C. capillifolia （6.30%）
D₃	24.25±1.25a	黄帚橐吾L. virgaurea （15.98%）、矮嵩草 C. alatauensis （10.70%）、高原早熟禾P. alpigena（10.03%）、线叶嵩草 C. capillifolia （9.38%）
D₄	25.00±0.82a	黄帚橐吾L. virgaurea （25.75%）、高原早熟禾P. alpigena （10.19%）、矮嵩草 C. alatauensis（9.53%）、线叶嵩草 C. capillifolia （5.96%）
D₅	22.25±0.48a	黄帚橐吾L. virgaurea （34.74%）、高原早熟禾P. alpigena （8.21%）、矮嵩草 C. alatauensis（6.56%）、线叶嵩草 C. capillifolia （5.31%）

斑块Patch	物种数Number of species	主要优势植物（重要值） The main dominant plants （importance value）
D₀	22.25±0.75a	高原早熟禾P. alpigena （13.65%）、矮嵩草 C. alatauensis （12.81%）、线叶嵩草C. capillifolia（7.96%）、钝裂银莲花A. obtusiloba （4.95%）
D₁	24.25±1.25a	高原早熟禾P. alpigena （10.85%）、矮嵩草 C. alatauensis （9.96%）、黄帚橐吾L. virgaurea（8.74%）、线叶嵩草 C. capillifolia （7.97%）
D₂	23.50±1.50a	高原早熟禾P. alpigena （13.74%）、矮嵩草 C. alatauensis （10.60%）、黄帚橐吾L. virgaurea（9.47%）、线叶嵩草 C. capillifolia （6.30%）
D₃	24.25±1.25a	黄帚橐吾L. virgaurea （15.98%）、矮嵩草 C. alatauensis （10.70%）、高原早熟禾P. alpigena（10.03%）、线叶嵩草 C. capillifolia （9.38%）
D₄	25.00±0.82a	黄帚橐吾L. virgaurea （25.75%）、高原早熟禾P. alpigena （10.19%）、矮嵩草 C. alatauensis（9.53%）、线叶嵩草 C. capillifolia （5.96%）
D₅	22.25±0.48a	黄帚橐吾L. virgaurea （34.74%）、高原早熟禾P. alpigena （8.21%）、矮嵩草 C. alatauensis（6.56%）、线叶嵩草 C. capillifolia （5.31%）

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