典型草原4种优势种牧草次生代谢物对亚洲小车蝗解毒酶活性的影响

doi:10.11686/cyxb2019128

草业学报 ›› 2020, Vol. 29 ›› Issue (1): 183-192.DOI: 10.11686/cyxb2019128

典型草原4种优势种牧草次生代谢物对亚洲小车蝗解毒酶活性的影响

陈德霞¹, 刘旭¹, 罗林华¹, 黄训兵^1,*, 吕慎金¹, 李广阅¹, 张泽华^2,3,*

1.临沂大学农林科学学院,山东临沂 276000;
2.中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193;
3.农业部锡林郭勒草原有害生物科学观测实验站,内蒙古锡林浩特 026000

收稿日期:2019-02-28 修回日期:2019-04-11 出版日期:2020-01-20 发布日期:2020-01-20
通讯作者: *E-mail: xunbingh@163.com, zhangzehua@caas.cn
作者简介:陈德霞(1999-),女,山东淄博人,在读本科。E-mail: dexialyu@163.com
基金资助:
国家级大学生创新训练项目(201910452021),山东省自然科学基金博士基金(ZR2019BC030),国家自然科学基金(31672485)和山东省现代农业产业技术体系牧草创新团队项目(SDAIT-23-10)资助

Plant secondary metabolites of four rangeland plant species and their effects on the activities of detoxification enzymes of Oedaleus asiaticus in a steppe grassland

CHEN De-xia¹, LIU Xu¹, LUO Lin-hua¹, HUANG Xun-bing^1,*, LÜ Shen-jin¹, LI Guang-yue¹, ZHANG Ze-hua^2,3,*

1.College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China;
2.State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3.Scientific Observation and Experimental Station of Pests in Xilin Gol Rangeland, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Xilinhot 026000, China

Received:2019-02-28 Revised:2019-04-11 Online:2020-01-20 Published:2020-01-20
Contact: *E-mail: xunbingh@163.com, zhangzehua@caas.cn

摘要/Abstract

摘要： 亚洲小车蝗是我国北方草原重要害虫,具有特定的食物选择性和适应性。为揭示其食物选择的形成机制,测定了典型草原克氏针茅、羊草、糙隐子草和冷蒿4种优势牧草的黄酮、萜类、生物碱、酚类和单宁5种次生代谢物含量,并研究了这些次生代谢物对亚洲小车蝗生长发育及关键解毒酶活性的影响。结果表明,冷蒿、羊草中5种次生代谢物含量最高(P<0.05),克氏针茅中含量最低(P<0.05);与冷蒿、羊草处理相比,克氏针茅处理的亚洲小车蝗存活率、发育速率、生活力最高(P<0.05),氧自由簇(ROS)浓度、解毒酶活性、保护酶活性最低(P<0.05);克氏针茅作为最优食物资源,最利于亚洲小车蝗的生长发育和发生。该研究对于揭示蝗虫-植物特异性关系的形成机理,指导亚洲小车蝗的科学防控具有重要意义。

关键词: 牧草, 蝗虫, 次生代谢物, 生长发育, 解毒酶

Abstract: Oedaleus asiaticus Bey-Bienko, a significant grasshopper pest species occurring in north China, has specific food preferences and adaptations. To explore the identity of the plant metabolites responsible for food preference, we measured the content of flavonoids, phenols, alkaloids, terpenoids, and tannins in the grassland species Stipa krylovii, Cleistogenes squarrosa, Leymus chinensis, and Artemisia frigida. The growth and detoxification enzyme activity of O. asiaticus feeding on the above four plant species with their different secondary metabolite profiles were also analyzed. The grass S. krylovii had low levels of secondary metabolites, while the grass L. chinensis and the herb A. frigida possessed the highest contents of the five measured plant secondary metabolites. Grasshoppers feeding on S. krylovii with low secondary metabolite levels, had greater growth and decreased reactive oxygen species (ROS) concentration and digestive enzyme activity then those feeding on L. chinensis and A. frigida. In summary, S. krylovii was the most favorable host plant among the plants tested, and enhanced the performance of the grasshopper species O. asiaticus. This study shows that grasshopper feeding preference is linked to low levels of secondary metabolites, and this information could aid in grasshopper control.

Key words: grass, grasshopper, plant secondary metabolites, growth, detoxification enzyme

陈德霞, 刘旭, 罗林华, 黄训兵, 吕慎金, 李广阅, 张泽华. 典型草原4种优势种牧草次生代谢物对亚洲小车蝗解毒酶活性的影响[J]. 草业学报, 2020, 29(1): 183-192.

CHEN De-xia, LIU Xu, LUO Lin-hua, HUANG Xun-bing, LÜ Shen-jin, LI Guang-yue, ZHANG Ze-hua. Plant secondary metabolites of four rangeland plant species and their effects on the activities of detoxification enzymes of Oedaleus asiaticus in a steppe grassland[J]. Acta Prataculturae Sinica, 2020, 29(1): 183-192.

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典型草原4种优势种牧草次生代谢物对亚洲小车蝗解毒酶活性的影响

Plant secondary metabolites of four rangeland plant species and their effects on the activities of detoxification enzymes of Oedaleus asiaticus in a steppe grassland

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