紫花苜蓿GLK基因家族鉴定及渗透胁迫下的表达分析

doi:10.11686/cyxb2024133

草业学报 ›› 2025, Vol. 34 ›› Issue (1): 174-190.DOI: 10.11686/cyxb2024133

• 研究论文 • 上一篇

紫花苜蓿GLK基因家族鉴定及渗透胁迫下的表达分析

马超¹(), 孙熙婧¹(), 冯雅岚², 周爽¹, 琚吉浩¹, 吴毅¹, 王添宁¹, 郭彬彬¹, 张均¹()

^1.河南科技大学农学院，河南洛阳 471000
^2.武昌理工学院生命科学学院，湖北武汉 430000

收稿日期:2024-04-18 修回日期:2024-06-05 出版日期:2025-01-20 发布日期:2024-11-04
通讯作者: 张均
作者简介:E-mail： zhangjun0105@126.com
马超（1984-），男，河南洛阳人，副教授，博士。E-mail： machao840508@163.com
孙熙婧（2003-），女，河南洛阳人，在读本科。E-mail： lierizhaoming2003@163.com。第一联系人：共同第一作者
基金资助:
国家自然科学基金(32372227);河南省高等学校青年骨干教师培养计划(2021GGJS050);河南省自然科学基金(242300421319);河南省科技研发计划联合基金项目(232103810021)

Genome-wide identification of the GLK gene family in alfalfa and their transcript profiles under osmotic stress

Chao MA¹(), Xi-jing SUN¹(), Ya-lan FENG², Shuang ZHOU¹, Ji-hao JU¹, Yi WU¹, Tian-ning WANG¹, Bin-bin GUO¹, Jun ZHANG¹()

^1.Agronomy College，Henan University of Science and Technology，Luoyang 471000，China
^2.College of Life Science，Wuchang University of Technology，Wuhan 430000，China

Received:2024-04-18 Revised:2024-06-05 Online:2025-01-20 Published:2024-11-04
Contact: Jun ZHANG
About author:First author contact:These authors contributed equally to this work.

摘要/Abstract

摘要：

GLK （Golden2-like或G2-like）属于GARP超家族，是植物生长发育过程中的重要转录因子，在调节植物叶绿体发育、叶绿素生物合成和非生物胁迫响应等方面发挥着重要作用。目前，GLK基因家族成员已在多个物种中被系统鉴定，但在四倍体紫花苜蓿全基因组水平上仍然知之甚少。利用生物信息学的方法，在“新疆大叶”紫花苜蓿基因组中鉴定到100个GLK基因（MsGLKs），并对其理化性质、染色体定位、系统进化关系、启动子顺式作用元件以及渗透胁迫和外源脱落酸（ABA）处理下的表达模式进行了分析。结果显示，100个MsGLK基因在32条染色体上不均匀分布，蛋白序列长度为201~860个氨基酸。根据系统发育分析结果，将MsGLK家族成员分为13个组。共线性分析表明，在紫花苜蓿基因组中共发现193个MsGLK基因重复事件，基因非同义替代数/同义替代数（Ka/Ks）分析显示，大部分重复基因对经历了纯化选择。MsGLK基因启动子的顺式作用元件广泛参与了植物生长发育、激素响应和胁迫反应。基因表达数据显示，12个基因的表达具有组织特异性，25个基因在所有组织中表达。RT-qPCR检测发现，MsGLK基因在干旱胁迫、盐胁迫和外源ABA处理下均有一定程度的响应。研究结果将为进一步探索 MsGLK基因的功能和紫花苜蓿抗逆性遗传改良提供参考。

关键词: 紫花苜蓿, GLK基因, 生物信息学, 渗透胁迫, 表达模式

Abstract:

GLK （Golden2-like or G2-like） transcription factors belong to the GARP superfamily， whose members play crucial roles in plant growth and development. Members of the GLK gene family play important roles in regulating plant chloroplast development， chlorophyll biosynthesis， and the abiotic stress response. The GLK gene family has been systematically identified in many plant species， but not yet in tetraploid alfalfa （Medicago sativa）. In this study， we used bioinformatics methods to identify 100 GLK genes （MsGLKs） in the genome of the alfalfa cultivar “Xinjiang Daye”. Further analyses were conducted to explore the physicochemical properties of their putative encoded products， chromosome localization， phylogenetic relationships， cis-acting elements in their promoter regions， and transcript profiles under osmotic stress and exogenous abscisic acid （ABA） treatment. The results showed that the 100 MsGLK genes were unevenly distributed on the 32 chromosomes， and encoded polypeptides with sequence lengths ranging from 201 to 860 amino acids. In a phylogenetic analysis， the MsGLK family members were divided into 13 groups. A collinearity analysis detected 193 MsGLK gene duplicates in the alfalfa genome， and a analysis of the ratio of the number of nonsynonymous substitutions per nonsynonymous site to the number of synonymous substitutions per synonymous site （Ka/Ks） revealed that most of the duplicated gene pairs have undergone purification selection. A range of cis-acting elements were detected in MsGLK gene promoter regions， and were involved in plant growth and development， hormone responses， and stress responses. Analyses of gene transcript profiles revealed that 12 MsGLKs showed tissue-specific expression patterns， and 25 MsGLKs were expressed in all tissues. Further RT-qPCR analyses revealed that some MsGLK genes were activated to some degree in response to drought stress， salt stress， and exogenous ABA treatment. The results of this study provide reference information for further research on the function of MsGLK genes and for the genetic improvement of stress resistance in alfalfa.

Key words: alfalfa, GLK gene, bioinformatics, osmotic stress, expression patterns

马超, 孙熙婧, 冯雅岚, 周爽, 琚吉浩, 吴毅, 王添宁, 郭彬彬, 张均. 紫花苜蓿GLK基因家族鉴定及渗透胁迫下的表达分析[J]. 草业学报, 2025, 34(1): 174-190.

Chao MA, Xi-jing SUN, Ya-lan FENG, Shuang ZHOU, Ji-hao JU, Yi WU, Tian-ning WANG, Bin-bin GUO, Jun ZHANG. Genome-wide identification of the GLK gene family in alfalfa and their transcript profiles under osmotic stress[J]. Acta Prataculturae Sinica, 2025, 34(1): 174-190.

图/表 10

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基因Gene	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
MsGLK10	CAGATGAAGTCGGATGAGCCT	GGTGGTGCGGTAAGTTGGTC
MsGLK17	TACCACAGCAAGGGAAGCAG	GAATGGAACGGCGATGAACT
MsGLK29	GCAAGTGGAGGCAGAGAAGC	GGGTGGGATGGTTTGAGGA
MsGLK33	AAACCTGCCTCTTTACCGATTC	GTTGCTCTTTCTTGCCCTCC
MsGLK36	GCAAGTGGAGGCAGAGAAGC	GGGTGGGATGGTTTGAGGA
MsGLK62	CATCACTCATCCCTTTCACCTC	CCTGCCAATCATTTCGCTT
MsGLK65	AACACTTCCGCTGTCCCAT	TCTCATTGCCGTCGTCATTT
MsGLK75	CTTCATCACTCATCCCTTTCACC	GCAGGCACCGCTCTCTACTT
MsGLK83	ATATCCAGGGTCATAGTATTTTCCA	CGTTGTTTTCAGTTCTTCCTCTTG
MsActin	ACTGGAATGGTGAAGGCTGG	TGACAATACCGTGCTCAATGG

基因Gene	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
MsGLK10	CAGATGAAGTCGGATGAGCCT	GGTGGTGCGGTAAGTTGGTC
MsGLK17	TACCACAGCAAGGGAAGCAG	GAATGGAACGGCGATGAACT
MsGLK29	GCAAGTGGAGGCAGAGAAGC	GGGTGGGATGGTTTGAGGA
MsGLK33	AAACCTGCCTCTTTACCGATTC	GTTGCTCTTTCTTGCCCTCC
MsGLK36	GCAAGTGGAGGCAGAGAAGC	GGGTGGGATGGTTTGAGGA
MsGLK62	CATCACTCATCCCTTTCACCTC	CCTGCCAATCATTTCGCTT
MsGLK65	AACACTTCCGCTGTCCCAT	TCTCATTGCCGTCGTCATTT
MsGLK75	CTTCATCACTCATCCCTTTCACC	GCAGGCACCGCTCTCTACTT
MsGLK83	ATATCCAGGGTCATAGTATTTTCCA	CGTTGTTTTCAGTTCTTCCTCTTG
MsActin	ACTGGAATGGTGAAGGCTGG	TGACAATACCGTGCTCAATGG

基因名 Gene name	基因登录号 Gene ID	PL （aa）	Mw （kDa）	pI	Sl	基因名 Gene name	基因登录号 Gene ID	PL （aa）	Mw （kDa）	pI	Sl
MsGLK1	MS.gene005086.t1	388	43.19	4.73	Nuc	MsGLK51	MS.gene76602.t1	352	39.86	5.75	Nuc
MsGLK2	MS.gene005866.t1	378	42.16	7.72	Nuc	MsGLK52	MS.gene55246.t1	352	40.14	5.54	Nuc
MsGLK3	MS.gene041038.t1	281	32.18	8.66	Nuc	MsGLK53	MS.gene028039.t1	277	31.24	6.03	Nuc
MsGLK4	MS.gene37289.t1	231	26.06	8.63	Nuc	MsGLK54	MS.gene045645.t1	353	40.08	5.75	Nuc
MsGLK5	MS.gene60590.t1	379	42.20	7.18	Nuc	MsGLK55	MS.gene070117.t1	277	31.32	5.82	Nuc
MsGLK6	MS.gene43963.t1	388	43.13	4.76	Nuc	MsGLK56	MS.gene032930.t1	352	39.83	5.75	Nuc
MsGLK7	MS.gene001686.t1	378	42.16	7.73	Nuc	MsGLK57	MS.gene87987.t1	393	44.55	6.20	Nuc
MsGLK8	MS.gene070698.t1	437	48.03	6.98	Nuc	MsGLK58	MS.gene85671.t1	308	34.34	9.37	Nuc
MsGLK9	MS.gene026358.t1	201	23.51	8.81	Nuc	MsGLK59	MS.gene68923.t1	393	44.48	6.16	Nuc
MsGLK10	MS.gene036427.t1	388	43.13	4.76	Nuc	MsGLK60	MS.gene042587.t1	393	44.45	6.07	Nuc
MsGLK11	MS.gene21397.t1	297	33.01	6.43	Nuc	MsGLK61	MS.gene66964.t1	393	44.48	6.16	Nuc
MsGLK12	MS.gene60862.t1	378	42.15	7.72	Nuc	MsGLK62	MS.gene018653.t1	489	53.80	5.42	Nuc
MsGLK13	MS.gene055776.t1	423	46.76	5.86	Nuc	MsGLK63	MS.gene017974.t1	359	40.43	6.92	Nuc
MsGLK14	MS.gene35408.t1	373	41.81	7.74	Nuc	MsGLK64	MS.gene017688.t1	301	32.94	6.56	Nuc
MsGLK15	MS.gene35289.t1	257	28.82	8.91	Nuc	MsGLK65	MS.gene013905.t1	281	30.65	5.79	Nuc
MsGLK16	MS.gene62480.t1	365	40.90	8.66	Cyt	MsGLK66	MS.gene99484.t1	350	39.92	5.48	Nuc
MsGLK17	MS.gene003164.t1	860	94.37	8.47	Chl	MsGLK67	MS.gene84188.t1	238	26.82	9.64	Nuc
MsGLK18	MS.gene003165.t1	330	36.16	6.19	Nuc	MsGLK68	MS.gene035191.t1	489	53.78	5.42	Nuc
MsGLK19	MS.gene043457.t1	423	46.73	5.86	Nuc	MsGLK69	MS.gene52648.t1	351	39.53	6.70	Nuc
MsGLK20	MS.gene84326.t1	373	41.81	7.74	Nuc	MsGLK70	MS.gene013966.t1	301	32.84	6.56	Nuc
MsGLK21	MS.gene073152.t1	313	35.17	6.98	Nuc	MsGLK71	MS.gene068055.t1	283	30.79	5.94	Nuc
MsGLK22	MS.gene066206.t1	375	41.71	8.37	Nuc	MsGLK72	MS.gene069022.t1	350	39.82	5.48	Nuc
MsGLK23	MS.gene01976.t1	331	36.21	6.23	Nuc	MsGLK73	MS.gene77355.t1	256	28.91	9.60	Nuc
MsGLK24	MS.gene01975.t1	382	41.62	8.62	Chl	MsGLK74	MS.gene024551.t1	439	48.79	5.11	Nuc
MsGLK25	MS.gene028866.t1	423	46.73	5.86	Nuc	MsGLK75	MS.gene23439.t1	489	53.78	5.42	Nuc
MsGLK26	MS.gene034456.t1	373	41.83	7.25	Nuc	MsGLK76	MS.gene04612.t1	359	40.43	6.92	Nuc
MsGLK27	MS.gene040544.t1	581	63.91	5.80	Nuc	MsGLK77	MS.gene58831.t1	298	32.76	6.56	Nuc
MsGLK28	MS.gene045711.t1	375	41.72	8.37	Nuc	MsGLK78	MS.gene77413.t1	350	39.94	5.56	Nuc
MsGLK29	MS.gene048293.t1	314	34.41	6.60	Nuc	MsGLK79	MS.gene94669.t1	256	28.93	9.78	Nuc
MsGLK30	MS.gene048294.t1	330	36.16	6.19	Nuc	MsGLK80	MS.gene050550.t1	439	48.79	5.11	Nuc
MsGLK31	MS.gene31432.t1	423	46.69	5.86	Nuc	MsGLK81	MS.gene96951.t1	489	53.73	5.37	Nuc
MsGLK32	MS.gene43040.t1	373	41.79	7.25	Nuc	MsGLK82	MS.gene41865.t1	283	30.79	5.94	Nuc
MsGLK33	MS.gene94587.t1	315	35.25	7.30	Nuc	MsGLK83	MS.gene95656.t1	350	39.85	5.48	Nuc
MsGLK34	MS.gene038958.t1	375	41.74	8.37	Nuc	MsGLK84	MS.gene95601.t1	238	26.83	9.64	Nuc
MsGLK35	MS.gene02861.t1	330	36.17	6.24	Nuc	MsGLK85	MS.gene036866.t1	434	47.87	6.66	Nuc
MsGLK36	MS.gene02867.t1	314	34.42	6.60	Nuc	MsGLK86	MS.gene61025.t1	352	39.45	6.50	Nuc
MsGLK37	MS.gene002965.t1	238	26.64	8.87	Nuc	MsGLK87	MS.gene068940.t1	345	38.77	6.64	Nuc
MsGLK38	MS.gene008318.t1	461	50.91	7.22	Nuc	MsGLK88	MS.gene060554.t1	434	47.88	6.66	Nuc
MsGLK39	MS.gene019228.t1	383	42.69	7.20	Chl	MsGLK89	MS.gene42755.t1	345	38.77	6.64	Nuc
MsGLK40	MS.gene061558.t1	426	47.13	7.99	Nuc	MsGLK90	MS.gene36713.t1	221	25.38	9.67	Chl
MsGLK41	MS.gene057902.t1	394	43.82	9.10	Nuc	MsGLK91	MS.gene87427.t1	650	71.83	5.55	Nuc
MsGLK42	MS.gene049714.t1	424	46.87	7.99	Nuc	MsGLK92	MS.gene36099.t1	346	38.76	6.64	Nuc
MsGLK43	MS.gene05012.t1	425	47.00	7.99	Nuc	MsGLK93	MS.gene88392.t1	302	33.85	8.12	Cyt
MsGLK44	MS.gene26549.t1	420	47.03	8.17	Nuc	MsGLK94	MS.gene056957.t1	363	40.53	9.07	Nuc
MsGLK45	MS.gene006790.t1	340	37.99	5.97	Nuc	MsGLK95	MS.gene056956.t1	434	47.87	6.66	Nuc
MsGLK46	MS.gene031499.t1	340	38.06	5.97	Nuc	MsGLK96	MS.gene038713.t1	434	47.87	6.66	Nuc
MsGLK47	MS.gene96791.t1	340	37.99	5.97	Nuc	MsGLK97	MS.gene066880.t1	346	38.90	6.68	Nuc
MsGLK48	MS.gene56537.t1	339	37.89	5.97	Nuc	MsGLK98	MS.gene00817.t1	238	26.61	8.87	Nuc
MsGLK49	MS.gene54226.t1	387	43.44	7.27	Nuc	MsGLK99	MS.gene02381.t1	238	26.66	8.87	Nuc
MsGLK50	MS.gene72865.t1	277	31.26	5.95	Nuc	MsGLK100	MS.gene072393.t1	351	39.62	6.73	Nuc

基因名 Gene name	基因登录号 Gene ID	PL （aa）	Mw （kDa）	pI	Sl	基因名 Gene name	基因登录号 Gene ID	PL （aa）	Mw （kDa）	pI	Sl
MsGLK1	MS.gene005086.t1	388	43.19	4.73	Nuc	MsGLK51	MS.gene76602.t1	352	39.86	5.75	Nuc
MsGLK2	MS.gene005866.t1	378	42.16	7.72	Nuc	MsGLK52	MS.gene55246.t1	352	40.14	5.54	Nuc
MsGLK3	MS.gene041038.t1	281	32.18	8.66	Nuc	MsGLK53	MS.gene028039.t1	277	31.24	6.03	Nuc
MsGLK4	MS.gene37289.t1	231	26.06	8.63	Nuc	MsGLK54	MS.gene045645.t1	353	40.08	5.75	Nuc
MsGLK5	MS.gene60590.t1	379	42.20	7.18	Nuc	MsGLK55	MS.gene070117.t1	277	31.32	5.82	Nuc
MsGLK6	MS.gene43963.t1	388	43.13	4.76	Nuc	MsGLK56	MS.gene032930.t1	352	39.83	5.75	Nuc
MsGLK7	MS.gene001686.t1	378	42.16	7.73	Nuc	MsGLK57	MS.gene87987.t1	393	44.55	6.20	Nuc
MsGLK8	MS.gene070698.t1	437	48.03	6.98	Nuc	MsGLK58	MS.gene85671.t1	308	34.34	9.37	Nuc
MsGLK9	MS.gene026358.t1	201	23.51	8.81	Nuc	MsGLK59	MS.gene68923.t1	393	44.48	6.16	Nuc
MsGLK10	MS.gene036427.t1	388	43.13	4.76	Nuc	MsGLK60	MS.gene042587.t1	393	44.45	6.07	Nuc
MsGLK11	MS.gene21397.t1	297	33.01	6.43	Nuc	MsGLK61	MS.gene66964.t1	393	44.48	6.16	Nuc
MsGLK12	MS.gene60862.t1	378	42.15	7.72	Nuc	MsGLK62	MS.gene018653.t1	489	53.80	5.42	Nuc
MsGLK13	MS.gene055776.t1	423	46.76	5.86	Nuc	MsGLK63	MS.gene017974.t1	359	40.43	6.92	Nuc
MsGLK14	MS.gene35408.t1	373	41.81	7.74	Nuc	MsGLK64	MS.gene017688.t1	301	32.94	6.56	Nuc
MsGLK15	MS.gene35289.t1	257	28.82	8.91	Nuc	MsGLK65	MS.gene013905.t1	281	30.65	5.79	Nuc
MsGLK16	MS.gene62480.t1	365	40.90	8.66	Cyt	MsGLK66	MS.gene99484.t1	350	39.92	5.48	Nuc
MsGLK17	MS.gene003164.t1	860	94.37	8.47	Chl	MsGLK67	MS.gene84188.t1	238	26.82	9.64	Nuc
MsGLK18	MS.gene003165.t1	330	36.16	6.19	Nuc	MsGLK68	MS.gene035191.t1	489	53.78	5.42	Nuc
MsGLK19	MS.gene043457.t1	423	46.73	5.86	Nuc	MsGLK69	MS.gene52648.t1	351	39.53	6.70	Nuc
MsGLK20	MS.gene84326.t1	373	41.81	7.74	Nuc	MsGLK70	MS.gene013966.t1	301	32.84	6.56	Nuc
MsGLK21	MS.gene073152.t1	313	35.17	6.98	Nuc	MsGLK71	MS.gene068055.t1	283	30.79	5.94	Nuc
MsGLK22	MS.gene066206.t1	375	41.71	8.37	Nuc	MsGLK72	MS.gene069022.t1	350	39.82	5.48	Nuc
MsGLK23	MS.gene01976.t1	331	36.21	6.23	Nuc	MsGLK73	MS.gene77355.t1	256	28.91	9.60	Nuc
MsGLK24	MS.gene01975.t1	382	41.62	8.62	Chl	MsGLK74	MS.gene024551.t1	439	48.79	5.11	Nuc
MsGLK25	MS.gene028866.t1	423	46.73	5.86	Nuc	MsGLK75	MS.gene23439.t1	489	53.78	5.42	Nuc
MsGLK26	MS.gene034456.t1	373	41.83	7.25	Nuc	MsGLK76	MS.gene04612.t1	359	40.43	6.92	Nuc
MsGLK27	MS.gene040544.t1	581	63.91	5.80	Nuc	MsGLK77	MS.gene58831.t1	298	32.76	6.56	Nuc
MsGLK28	MS.gene045711.t1	375	41.72	8.37	Nuc	MsGLK78	MS.gene77413.t1	350	39.94	5.56	Nuc
MsGLK29	MS.gene048293.t1	314	34.41	6.60	Nuc	MsGLK79	MS.gene94669.t1	256	28.93	9.78	Nuc
MsGLK30	MS.gene048294.t1	330	36.16	6.19	Nuc	MsGLK80	MS.gene050550.t1	439	48.79	5.11	Nuc
MsGLK31	MS.gene31432.t1	423	46.69	5.86	Nuc	MsGLK81	MS.gene96951.t1	489	53.73	5.37	Nuc
MsGLK32	MS.gene43040.t1	373	41.79	7.25	Nuc	MsGLK82	MS.gene41865.t1	283	30.79	5.94	Nuc
MsGLK33	MS.gene94587.t1	315	35.25	7.30	Nuc	MsGLK83	MS.gene95656.t1	350	39.85	5.48	Nuc
MsGLK34	MS.gene038958.t1	375	41.74	8.37	Nuc	MsGLK84	MS.gene95601.t1	238	26.83	9.64	Nuc
MsGLK35	MS.gene02861.t1	330	36.17	6.24	Nuc	MsGLK85	MS.gene036866.t1	434	47.87	6.66	Nuc
MsGLK36	MS.gene02867.t1	314	34.42	6.60	Nuc	MsGLK86	MS.gene61025.t1	352	39.45	6.50	Nuc
MsGLK37	MS.gene002965.t1	238	26.64	8.87	Nuc	MsGLK87	MS.gene068940.t1	345	38.77	6.64	Nuc
MsGLK38	MS.gene008318.t1	461	50.91	7.22	Nuc	MsGLK88	MS.gene060554.t1	434	47.88	6.66	Nuc
MsGLK39	MS.gene019228.t1	383	42.69	7.20	Chl	MsGLK89	MS.gene42755.t1	345	38.77	6.64	Nuc
MsGLK40	MS.gene061558.t1	426	47.13	7.99	Nuc	MsGLK90	MS.gene36713.t1	221	25.38	9.67	Chl
MsGLK41	MS.gene057902.t1	394	43.82	9.10	Nuc	MsGLK91	MS.gene87427.t1	650	71.83	5.55	Nuc
MsGLK42	MS.gene049714.t1	424	46.87	7.99	Nuc	MsGLK92	MS.gene36099.t1	346	38.76	6.64	Nuc
MsGLK43	MS.gene05012.t1	425	47.00	7.99	Nuc	MsGLK93	MS.gene88392.t1	302	33.85	8.12	Cyt
MsGLK44	MS.gene26549.t1	420	47.03	8.17	Nuc	MsGLK94	MS.gene056957.t1	363	40.53	9.07	Nuc
MsGLK45	MS.gene006790.t1	340	37.99	5.97	Nuc	MsGLK95	MS.gene056956.t1	434	47.87	6.66	Nuc
MsGLK46	MS.gene031499.t1	340	38.06	5.97	Nuc	MsGLK96	MS.gene038713.t1	434	47.87	6.66	Nuc
MsGLK47	MS.gene96791.t1	340	37.99	5.97	Nuc	MsGLK97	MS.gene066880.t1	346	38.90	6.68	Nuc
MsGLK48	MS.gene56537.t1	339	37.89	5.97	Nuc	MsGLK98	MS.gene00817.t1	238	26.61	8.87	Nuc
MsGLK49	MS.gene54226.t1	387	43.44	7.27	Nuc	MsGLK99	MS.gene02381.t1	238	26.66	8.87	Nuc
MsGLK50	MS.gene72865.t1	277	31.26	5.95	Nuc	MsGLK100	MS.gene072393.t1	351	39.62	6.73	Nuc

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紫花苜蓿GLK基因家族鉴定及渗透胁迫下的表达分析

Genome-wide identification of the GLK gene family in alfalfa and their transcript profiles under osmotic stress

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