结缕草DREB基因家族的鉴定及非生物胁迫下的表达模式分析

doi:10.11686/cyxb2024257

摘要/Abstract

摘要：

DREB转录因子属于AP2/ERF超家族的一个分支，在植物生长发育以及胁迫应答中发挥至关重要的调控作用。而在我国优良的暖季型草坪草结缕草中关于DREB家族基因的系统分析目前还未见报道。本研究从结缕草全基因组中共鉴定到64个ZjDREB基因，编码的氨基酸为105~984 aa，分子量为11.42~107.04 kDa，理论等电点为4.09~11.95。系统发育分析显示ZjDREB基因分为A₁~A₆共6组。蛋白序列的保守基序分析表明motif 1、motif 2和motif 3在所有ZjDREB蛋白序列中的分布相对保守，且其中的61个ZjDREB基因在结缕草14条染色体上呈不均匀分布。对ZjDREB基因的共线性分析中共鉴定到3个片段重复基因对，且基因对的非同义替代与同义替代的比值（Ka/Ks）均小于1，表明其在基因扩张中受纯化选择。顺式作用元件分析显示ZjDREB A₁和A₂组基因启动子区域含有多个与植物激素和非生物胁迫相关的作用元件。利用qRT-PCR对A₁和A₂组基因在低温、脱落酸、干旱和盐胁迫下的表达模式进行分析，结果显示A₁组基因在低温和脱落酸胁迫处理后上调表达，其中ZjDREB3上调幅度最大；A₂组基因在干旱和高盐胁迫后表现出不同的表达模式，其中ZjDREB4的上调幅度最大。本研究结果为进一步探究结缕草ZjDREB基因的功能奠定了基础，也为结缕草的遗传改良提供了新的线索。

关键词: 结缕草, DREB转录因子, 系统进化, 非生物胁迫, 表达模式

Abstract:

DREB transcription factors belong to the AP2/ERF superfamily， and play important roles in plant development and stress responses. However， a systematic analysis of DREB genes in Zoysia japonica， which is a warm-season turfgrass native to China has not been reported. In this study， we identified 64 ZjDREBs from Z. japonica， encoding polypeptides of 105 to 984 amino acids， with molecular weights ranging from 11.42 to 107.04 kDa and theoretical isoelectric points ranging from 4.09 to 11.95. The phylogenetic analysis of 64 ZjDREBs showed that they were divided into six groups from A₁ to A₆. A conserved motif analysis of ZjDREB protein sequences revealed that motif1， motif2， and motif3 were relatively highly conserved. Sixty-one ZjDREBs were unevenly distributed on 14 chromosomes of Z. japonica. By colinearity analysis， we identified three pairs of segmentally duplicated ZjDREB genes. The non-synonymous∶synonymous （Ka/Ks） was <1， indicating that this gene family might have been subject to purifying selection during its expansion. Analyses of the promoter regions of ZjDREBs in the A₁ and A₂ groups revealed multiple cis-acting elements related to plant hormones and abiotic stresses. The expression profiles of ZjDREB genes in the A₁ and A₂ groups under cold， abscisic acid （ABA）， drought， and salt stress were detected by quantitative real-time polymerase chain reaction （qRT-PCR）. The A₁ group genes were up-regulated by cold and ABA treatments， and ZjDREB3 was significantly up-regulated. The A₂ group genes showed different expression profiles under drought and salt stress， and ZjDREB4 was significantly up-regulated. Together， these results lay a foundation for further exploration of the functions of ZjDREB genes and provide avenues for the genetic improvement of Z. japonica.

Key words: Zoysia japonica, DREB transcription factor, phylogenetic evolution, abiotic stress, expression profiling

左志芳, 李永龙, 魏雨佳, 周生辉, 李岩, 杨国锋. 结缕草DREB基因家族的鉴定及非生物胁迫下的表达模式分析[J]. 草业学报, 2025, 34(5): 74-88.

Zhi-fang ZUO, Yong-long LI, Yu-jia WEI, Sheng-hui ZHOU, Yan LI, Guo-feng YANG. Identification of DREB genes from Zoysia japonica and their transcript profiles in response to abiotic stress[J]. Acta Prataculturae Sinica, 2025, 34(5): 74-88.

图/表 9

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名称Name	寡核苷酸Oligonucleotides （5′-3′）	组Group	用途Purpose
ZjDREB3-r-F	AAGGGGCTGAGGGTGAGTA	A₁	用于ZjDREB A₁组基因的qRT-PCR分析 For the qRT-PCR of ZjDREB A₁ group genes
ZjDREB3-r-R	GATGCGGGACTTCTTGTTG	A₁
ZjDREB12-r-F	GTCCGATCAAGGAGGAGATG	A₁
ZjDREB12-r-R	GAAGGTGCCAAGCCAGAG	A₁
ZjDREB19-r-F	AATGCCGGACCTGTTTTCTA	A₁
ZjDREB19-r-R	TAGCTCCAGAGCCTGACCTC	A₁
ZjDREB25-r-F	ACCTGCTTTCTAGCGAGACG	A₁
ZjDREB25-r-R	GCTCCAAAGCTTGACCTCAC	A₁
ZjDREB35-r-F	GAGTACGCCACCGTCATGT	A₁
ZjDREB35-r-R	GATGCGGGATTTCTTGTTG	A₁
ZjDREB1-r-F	GCACGAACCCAAGATGAAAT	A₂	用于ZjDREB A₂组基因的qRT-PCR分析 For the qRT-PCR of ZjDREB A₂ group genes
ZjDREB1-r-R	GCCACCTTCATTGGTTCTGT	A₂
ZjDREB4-r-F	GGCTGCAGGAGATCAAGAAC	A₂
ZjDREB4-r-R	CTGGAAGCACGATATTGACG	A₂
ZjDREB9-r-F	AAGTTCTGCTGATGCCACCT	A₂
ZjDREB9-r-R	ATGTGGATGCTTCCCAAATC	A₂
ZjDREB11-r-F	AGGGAGTTCTTGCAGCAGAT	A₂
ZjDREB11-r-R	GGCAAGTAGCTCCCAAACTG	A₂
ZjDREB13-r-F	TGAATTCTGGAGCGGAGTCT	A₂
ZjDREB13-r-R	GTCAGCTTCCATCATGCTCA	A₂
ZjDREB21-r-F	CAAGGGCTGCATGAAGGG	A₂
ZjDREB21-r-R	ATCTCGGCGACCCACTTG	A₂
ZjDREB24-r-F	ATGAAGGGCAAGGGCGG	A₂
ZjDREB24-r-R	GACCCACTTGCCCCAGG	A₂
ZjACT-F	AAGGCCAACAGGGAGAAAAT	-	结缕草内参基因 Inner reference gene of Z. japonica
ZjACT-R	GATAGCATGGGGAAGTGCAT	-	结缕草内参基因 Inner reference gene of Z. japonica

名称Name	寡核苷酸Oligonucleotides （5′-3′）	组Group	用途Purpose
ZjDREB3-r-F	AAGGGGCTGAGGGTGAGTA	A₁	用于ZjDREB A₁组基因的qRT-PCR分析 For the qRT-PCR of ZjDREB A₁ group genes
ZjDREB3-r-R	GATGCGGGACTTCTTGTTG	A₁
ZjDREB12-r-F	GTCCGATCAAGGAGGAGATG	A₁
ZjDREB12-r-R	GAAGGTGCCAAGCCAGAG	A₁
ZjDREB19-r-F	AATGCCGGACCTGTTTTCTA	A₁
ZjDREB19-r-R	TAGCTCCAGAGCCTGACCTC	A₁
ZjDREB25-r-F	ACCTGCTTTCTAGCGAGACG	A₁
ZjDREB25-r-R	GCTCCAAAGCTTGACCTCAC	A₁
ZjDREB35-r-F	GAGTACGCCACCGTCATGT	A₁
ZjDREB35-r-R	GATGCGGGATTTCTTGTTG	A₁
ZjDREB1-r-F	GCACGAACCCAAGATGAAAT	A₂	用于ZjDREB A₂组基因的qRT-PCR分析 For the qRT-PCR of ZjDREB A₂ group genes
ZjDREB1-r-R	GCCACCTTCATTGGTTCTGT	A₂
ZjDREB4-r-F	GGCTGCAGGAGATCAAGAAC	A₂
ZjDREB4-r-R	CTGGAAGCACGATATTGACG	A₂
ZjDREB9-r-F	AAGTTCTGCTGATGCCACCT	A₂
ZjDREB9-r-R	ATGTGGATGCTTCCCAAATC	A₂
ZjDREB11-r-F	AGGGAGTTCTTGCAGCAGAT	A₂
ZjDREB11-r-R	GGCAAGTAGCTCCCAAACTG	A₂
ZjDREB13-r-F	TGAATTCTGGAGCGGAGTCT	A₂
ZjDREB13-r-R	GTCAGCTTCCATCATGCTCA	A₂
ZjDREB21-r-F	CAAGGGCTGCATGAAGGG	A₂
ZjDREB21-r-R	ATCTCGGCGACCCACTTG	A₂
ZjDREB24-r-F	ATGAAGGGCAAGGGCGG	A₂
ZjDREB24-r-R	GACCCACTTGCCCCAGG	A₂
ZjACT-F	AAGGCCAACAGGGAGAAAAT	-	结缕草内参基因 Inner reference gene of Z. japonica
ZjACT-R	GATAGCATGGGGAAGTGCAT	-	结缕草内参基因 Inner reference gene of Z. japonica

基因号 Gene ID	基因名称 Gene name	期望值 E-value	蛋白质长度 Protein length （aa）	分子量 MW （kDa）	等电点 pI	组 Group	染色体 Chromosome
Zjn_sc00016.1.g00280.1.sm.mkhc	ZjDREB1	2.90e^-15	338	37.29	4.58	A₂	Chr18
Zjn_sc00091.1.g00300.1.sm.mk	ZjDREB2	3.30e^-15	282	29.35	6.02	A₆	Chr13
Zjn_sc00141.1.g00460.1.sm.mk	ZjDREB3	3.80e^-15	216	23.53	4.52	A₁	Zjn_sc00141.1
Zjn_sc00003.1.g10920.1.am.mk	ZjDREB4	4.10e^-15	500	53.29	10.24	A₂	Chr02
Zjn_sc00015.1.g00570.1.sm.mk	ZjDREB5	4.80e^-15	287	30.25	6.03	A₆	Chr14
Zjn_sc00056.1.g04220.1.am.mk	ZjDREB6	5.50e^-15	428	45.60	8.34	A₄	Chr11
Zjn_sc00004.1.g06540.1.am.mk	ZjDREB7	6.40e^-15	375	40.02	8.43	A₄	Chr12
Zjn_sc00022.1.g06610.1.am.mk	ZjDREB8	7.40e^-15	256	27.32	4.92	A₄	Chr08
Zjn_sc00005.1.g05270.1.sm.mkhc	ZjDREB9	7.60e^-15	257	28.87	5.49	A₂	Chr06
Zjn_sc00007.1.g10280.1.am.mk	ZjDREB10	7.80e^-15	285	29.80	4.45	A₄	Chr07
Zjn_sc00002.1.g12900.1.sm.mk	ZjDREB11	7.90e^-15	311	33.08	6.42	A₂	Chr01
Zjn_sc00050.1.g01870.1.am.mk	ZjDREB12	8.30e^-15	317	34.00	4.19	A₁	Chr20
Zjn_sc00098.1.g00890.1.sm.mkhc	ZjDREB13	9.60e^-15	337	36.46	4.47	A₂	Zjn_sc00098.1
Zjn_sc00003.1.g09710.1.sm.mk	ZjDREB14	1.50e^-14	199	21.34	7.16	A₆	Chr02
Zjn_sc00007.1.g04460.1.am.mk	ZjDREB15	4.00e^-14	330	35.52	6.97	A₆	Chr07
Zjn_sc00022.1.g00980.1.am.mk	ZjDREB16	4.30e^-14	340	36.38	6.80	A₆	Chr08
Zjn_sc00022.1.g06640.1.am.mk	ZjDREB17	6.00e^-14	361	38.67	9.00	A₄	Chr08
Zjn_sc00047.1.g02470.1.sm.mk	ZjDREB18	6.70e^-14	205	21.63	4.65	A₄	Chr08
Zjn_sc00007.1.g09230.1.am.mk	ZjDREB19	7.00e^-14	326	35.41	8.74	A₁	Chr07
Zjn_sc00045.1.g00300.1.am.mk	ZjDREB20	7.70e^-14	242	25.19	4.57	A₄	Chr07
Zjn_sc00078.1.g03540.1.am.mk	ZjDREB21	7.90e^-14	236	25.61	7.32	A₂	Chr13
Zjn_sc00032.1.g00670.1.am.mk	ZjDREB22	7.90e^-14	281	30.91	8.88	A₆	Chr08
Zjn_sc00007.1.g10310.1.sm.mk	ZjDREB23	9.00e^-14	263	27.37	6.24	A₄	Chr07
Zjn_sc00015.1.g08330.1.am.mk	ZjDREB24	9.50e^-14	267	29.18	8.42	A₂	Chr14
Zjn_sc00022.1.g05590.1.am.mk	ZjDREB25	9.60e^-14	440	47.01	8.98	A₁	Chr08
Zjn_sc00056.1.g04250.1.am.mk	ZjDREB26	1.00e^-13	273	29.35	8.11	A₄	Chr11
Zjn_sc00053.1.g02230.1.am.mk	ZjDREB27	1.20e^-13	984	107.04	11.22	A₄	Zjn_sc00053.1
Zjn_sc00022.1.g06600.1.am.mk	ZjDREB28	1.40e^-13	475	50.95	8.41	A₄	Chr08
Zjn_sc00012.1.g01050.1.am.mk	ZjDREB29	1.50e^-13	259	28.55	10.16	A₆	Chr17
Zjn_sc00018.1.g05900.1.am.mk	ZjDREB30	1.60e^-13	293	31.03	6.25	A₆	Chr20
Zjn_sc00007.1.g11280.1.sm.mk	ZjDREB31	1.60e^-13	286	30.57	6.52	A₆	Chr07
Zjn_sc00002.1.g16790.1.am.mk	ZjDREB32	1.80e^-13	105	11.42	10.14	A₄	Chr01
Zjn_sc00027.1.g01240.1.am.mk	ZjDREB33	2.70e^-13	373	40.79	10.62	A₆	Chr18
Zjn_sc00016.1.g00480.1.am.mk	ZjDREB34	3.90e^-13	296	31.65	11.73	A₃	Chr18
Zjn_sc00067.1.g01840.1.am.mk	ZjDREB35	4.00e^-13	245	26.85	7.53	A₁	Chr19
Zjn_sc00069.1.g03570.1.am.mk	ZjDREB36	6.10e^-13	603	66.31	8.24	A₄	Chr20
Zjn_sc00022.1.g07750.1.am.mk	ZjDREB37	7.30e^-13	654	70.65	10.01	A₆	Chr08
Zjn_sc00004.1.g01090.1.sm.mk	ZjDREB38	1.10e^-12	213	23.44	5.60	A₅	Chr19
Zjn_sc00043.1.g03350.1.am.mk	ZjDREB39	1.10e^-12	225	24.22	6.54	A₄	Chr05
Zjn_sc00143.1.g00970.1.am.mk	ZjDREB40	1.10e^-12	593	63.86	10.35	A₆	Chr19
Zjn_sc00004.1.g05180.1.am.mk	ZjDREB41	2.40e^-12	562	59.14	10.76	A₆	Chr12
Zjn_sc00049.1.g04190.1.am.mk	ZjDREB42	2.90e^-12	550	59.72	10.30	A₆	Chr20
Zjn_sc00056.1.g02990.1.sm.mk	ZjDREB43	3.70e^-12	430	45.93	9.52	A₆	Chr11
Zjn_sc00056.1.g04230.1.am.mk	ZjDREB44	4.20e^-12	240	25.55	4.67	A₄	Chr11
Zjn_sc00045.1.g01190.1.am.mk	ZjDREB45	6.60e^-12	216	23.56	9.88	A₆	Chr07
Zjn_sc00049.1.g01960.1.sm.mk	ZjDREB46	6.70e^-12	169	18.24	6.80	A₆	Chr20
Zjn_sc00004.1.g01940.1.am.mk	ZjDREB47	7.80e^-12	164	17.89	6.78	A₆	Chr19
Zjn_sc00002.1.g07220.1.am.mk	ZjDREB48	8.70e^-12	239	24.73	5.68	A₅	Chr01
Zjn_sc00003.1.g05800.1.sm.mk	ZjDREB49	1.20e^-11	233	24.33	7.01	A₅	Chr02
Zjn_sc00008.1.g05900.1.am.mk	ZjDREB50	1.80e^-11	404	43.84	9.18	A₆	Chr19
Zjn_sc00007.1.g03660.1.sm.mk	ZjDREB51	2.70e^-11	239	25.28	4.84	A₅	Chr07
Zjn_sc00023.1.g04740.1.sm.mk	ZjDREB52	5.50e^-11	234	25.45	10.28	A₅	Chr11
Zjn_sc00069.1.g02080.1.am.mk	ZjDREB53	6.00e^-11	516	56.67	10.48	A₆	Chr20
Zjn_sc00004.1.g02760.1.am.mk	ZjDREB54	6.30e^-11	716	76.32	11.95	A₅	Chr19
Zjn_sc00049.1.g03550.1.sm.mk	ZjDREB55	3.10e^-10	230	23.86	4.09	A₅	Chr20
Zjn_sc00004.1.g00470.1.sm.mkhc	ZjDREB56	6.90e^-10	585	64.46	9.36	A₅	Chr19
Zjn_sc00066.1.g02740.1.am.mk	ZjDREB57	7.10e^-10	242	26.02	6.68	A₅	Chr08
Zjn_sc00022.1.g05620.1.am.mk	ZjDREB58	1.10e^-09	422	45.15	9.13	A₄	Chr08
Zjn_sc00009.1.g04230.1.am.mk	ZjDREB59	1.50e^-09	270	29.90	10.70	A₅	Chr05
Zjn_sc00007.1.g09250.1.sm.mk	ZjDREB60	1.60e^-09	237	24.53	4.68	A₄	Chr07
Zjn_sc00007.1.g02920.1.sm.mk	ZjDREB61	1.70e^-09	209	22.48	4.78	A₅	Chr07
Zjn_sc00004.1.g07680.1.am.mk	ZjDREB62	2.00e^-09	247	25.82	9.15	A₄	Chr12
Zjn_sc00034.1.g04900.1.sm.mk	ZjDREB63	8.30e^-09	181	18.98	8.53	A₅	Chr06
Zjn_sc00025.1.g02120.1.am.mk	ZjDREB64	8.60e^-07	398	42.36	8.35	A₅	Chr18

基因号 Gene ID	基因名称 Gene name	期望值 E-value	蛋白质长度 Protein length （aa）	分子量 MW （kDa）	等电点 pI	组 Group	染色体 Chromosome
Zjn_sc00016.1.g00280.1.sm.mkhc	ZjDREB1	2.90e^-15	338	37.29	4.58	A₂	Chr18
Zjn_sc00091.1.g00300.1.sm.mk	ZjDREB2	3.30e^-15	282	29.35	6.02	A₆	Chr13
Zjn_sc00141.1.g00460.1.sm.mk	ZjDREB3	3.80e^-15	216	23.53	4.52	A₁	Zjn_sc00141.1
Zjn_sc00003.1.g10920.1.am.mk	ZjDREB4	4.10e^-15	500	53.29	10.24	A₂	Chr02
Zjn_sc00015.1.g00570.1.sm.mk	ZjDREB5	4.80e^-15	287	30.25	6.03	A₆	Chr14
Zjn_sc00056.1.g04220.1.am.mk	ZjDREB6	5.50e^-15	428	45.60	8.34	A₄	Chr11
Zjn_sc00004.1.g06540.1.am.mk	ZjDREB7	6.40e^-15	375	40.02	8.43	A₄	Chr12
Zjn_sc00022.1.g06610.1.am.mk	ZjDREB8	7.40e^-15	256	27.32	4.92	A₄	Chr08
Zjn_sc00005.1.g05270.1.sm.mkhc	ZjDREB9	7.60e^-15	257	28.87	5.49	A₂	Chr06
Zjn_sc00007.1.g10280.1.am.mk	ZjDREB10	7.80e^-15	285	29.80	4.45	A₄	Chr07
Zjn_sc00002.1.g12900.1.sm.mk	ZjDREB11	7.90e^-15	311	33.08	6.42	A₂	Chr01
Zjn_sc00050.1.g01870.1.am.mk	ZjDREB12	8.30e^-15	317	34.00	4.19	A₁	Chr20
Zjn_sc00098.1.g00890.1.sm.mkhc	ZjDREB13	9.60e^-15	337	36.46	4.47	A₂	Zjn_sc00098.1
Zjn_sc00003.1.g09710.1.sm.mk	ZjDREB14	1.50e^-14	199	21.34	7.16	A₆	Chr02
Zjn_sc00007.1.g04460.1.am.mk	ZjDREB15	4.00e^-14	330	35.52	6.97	A₆	Chr07
Zjn_sc00022.1.g00980.1.am.mk	ZjDREB16	4.30e^-14	340	36.38	6.80	A₆	Chr08
Zjn_sc00022.1.g06640.1.am.mk	ZjDREB17	6.00e^-14	361	38.67	9.00	A₄	Chr08
Zjn_sc00047.1.g02470.1.sm.mk	ZjDREB18	6.70e^-14	205	21.63	4.65	A₄	Chr08
Zjn_sc00007.1.g09230.1.am.mk	ZjDREB19	7.00e^-14	326	35.41	8.74	A₁	Chr07
Zjn_sc00045.1.g00300.1.am.mk	ZjDREB20	7.70e^-14	242	25.19	4.57	A₄	Chr07
Zjn_sc00078.1.g03540.1.am.mk	ZjDREB21	7.90e^-14	236	25.61	7.32	A₂	Chr13
Zjn_sc00032.1.g00670.1.am.mk	ZjDREB22	7.90e^-14	281	30.91	8.88	A₆	Chr08
Zjn_sc00007.1.g10310.1.sm.mk	ZjDREB23	9.00e^-14	263	27.37	6.24	A₄	Chr07
Zjn_sc00015.1.g08330.1.am.mk	ZjDREB24	9.50e^-14	267	29.18	8.42	A₂	Chr14
Zjn_sc00022.1.g05590.1.am.mk	ZjDREB25	9.60e^-14	440	47.01	8.98	A₁	Chr08
Zjn_sc00056.1.g04250.1.am.mk	ZjDREB26	1.00e^-13	273	29.35	8.11	A₄	Chr11
Zjn_sc00053.1.g02230.1.am.mk	ZjDREB27	1.20e^-13	984	107.04	11.22	A₄	Zjn_sc00053.1
Zjn_sc00022.1.g06600.1.am.mk	ZjDREB28	1.40e^-13	475	50.95	8.41	A₄	Chr08
Zjn_sc00012.1.g01050.1.am.mk	ZjDREB29	1.50e^-13	259	28.55	10.16	A₆	Chr17
Zjn_sc00018.1.g05900.1.am.mk	ZjDREB30	1.60e^-13	293	31.03	6.25	A₆	Chr20
Zjn_sc00007.1.g11280.1.sm.mk	ZjDREB31	1.60e^-13	286	30.57	6.52	A₆	Chr07
Zjn_sc00002.1.g16790.1.am.mk	ZjDREB32	1.80e^-13	105	11.42	10.14	A₄	Chr01
Zjn_sc00027.1.g01240.1.am.mk	ZjDREB33	2.70e^-13	373	40.79	10.62	A₆	Chr18
Zjn_sc00016.1.g00480.1.am.mk	ZjDREB34	3.90e^-13	296	31.65	11.73	A₃	Chr18
Zjn_sc00067.1.g01840.1.am.mk	ZjDREB35	4.00e^-13	245	26.85	7.53	A₁	Chr19
Zjn_sc00069.1.g03570.1.am.mk	ZjDREB36	6.10e^-13	603	66.31	8.24	A₄	Chr20
Zjn_sc00022.1.g07750.1.am.mk	ZjDREB37	7.30e^-13	654	70.65	10.01	A₆	Chr08
Zjn_sc00004.1.g01090.1.sm.mk	ZjDREB38	1.10e^-12	213	23.44	5.60	A₅	Chr19
Zjn_sc00043.1.g03350.1.am.mk	ZjDREB39	1.10e^-12	225	24.22	6.54	A₄	Chr05
Zjn_sc00143.1.g00970.1.am.mk	ZjDREB40	1.10e^-12	593	63.86	10.35	A₆	Chr19
Zjn_sc00004.1.g05180.1.am.mk	ZjDREB41	2.40e^-12	562	59.14	10.76	A₆	Chr12
Zjn_sc00049.1.g04190.1.am.mk	ZjDREB42	2.90e^-12	550	59.72	10.30	A₆	Chr20
Zjn_sc00056.1.g02990.1.sm.mk	ZjDREB43	3.70e^-12	430	45.93	9.52	A₆	Chr11
Zjn_sc00056.1.g04230.1.am.mk	ZjDREB44	4.20e^-12	240	25.55	4.67	A₄	Chr11
Zjn_sc00045.1.g01190.1.am.mk	ZjDREB45	6.60e^-12	216	23.56	9.88	A₆	Chr07
Zjn_sc00049.1.g01960.1.sm.mk	ZjDREB46	6.70e^-12	169	18.24	6.80	A₆	Chr20
Zjn_sc00004.1.g01940.1.am.mk	ZjDREB47	7.80e^-12	164	17.89	6.78	A₆	Chr19
Zjn_sc00002.1.g07220.1.am.mk	ZjDREB48	8.70e^-12	239	24.73	5.68	A₅	Chr01
Zjn_sc00003.1.g05800.1.sm.mk	ZjDREB49	1.20e^-11	233	24.33	7.01	A₅	Chr02
Zjn_sc00008.1.g05900.1.am.mk	ZjDREB50	1.80e^-11	404	43.84	9.18	A₆	Chr19
Zjn_sc00007.1.g03660.1.sm.mk	ZjDREB51	2.70e^-11	239	25.28	4.84	A₅	Chr07
Zjn_sc00023.1.g04740.1.sm.mk	ZjDREB52	5.50e^-11	234	25.45	10.28	A₅	Chr11
Zjn_sc00069.1.g02080.1.am.mk	ZjDREB53	6.00e^-11	516	56.67	10.48	A₆	Chr20
Zjn_sc00004.1.g02760.1.am.mk	ZjDREB54	6.30e^-11	716	76.32	11.95	A₅	Chr19
Zjn_sc00049.1.g03550.1.sm.mk	ZjDREB55	3.10e^-10	230	23.86	4.09	A₅	Chr20
Zjn_sc00004.1.g00470.1.sm.mkhc	ZjDREB56	6.90e^-10	585	64.46	9.36	A₅	Chr19
Zjn_sc00066.1.g02740.1.am.mk	ZjDREB57	7.10e^-10	242	26.02	6.68	A₅	Chr08
Zjn_sc00022.1.g05620.1.am.mk	ZjDREB58	1.10e^-09	422	45.15	9.13	A₄	Chr08
Zjn_sc00009.1.g04230.1.am.mk	ZjDREB59	1.50e^-09	270	29.90	10.70	A₅	Chr05
Zjn_sc00007.1.g09250.1.sm.mk	ZjDREB60	1.60e^-09	237	24.53	4.68	A₄	Chr07
Zjn_sc00007.1.g02920.1.sm.mk	ZjDREB61	1.70e^-09	209	22.48	4.78	A₅	Chr07
Zjn_sc00004.1.g07680.1.am.mk	ZjDREB62	2.00e^-09	247	25.82	9.15	A₄	Chr12
Zjn_sc00034.1.g04900.1.sm.mk	ZjDREB63	8.30e^-09	181	18.98	8.53	A₅	Chr06
Zjn_sc00025.1.g02120.1.am.mk	ZjDREB64	8.60e^-07	398	42.36	8.35	A₅	Chr18

基因组位置Location on genome				基因组位置Location on genome				非同义替代/同义替代Ka/Ks
基因 Gene	染色体 Chromosome	起始位置 Start	终止位置 End	基因 Gene	染色体 Chromosome	起始位置 Start	终止位置 End	非同义替代/同义替代Ka/Ks
ZjDREB15	Chr07	18308254	18309246	ZjDREB16	Chr08	19055071	19056093	0.1684
ZjDREB46	Chr20	804556	804638	ZjDREB47	Chr19	1618296	1618378	0.4207
ZjDREB57	Chr08	19874486	19875140	ZjDREB61	Chr07	18982769	18983398	0.2172