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草业学报 ›› 2023, Vol. 32 ›› Issue (11): 93-105.DOI: 10.11686/cyxb2023020

• 研究论文 • 上一篇    下一篇

荒漠草原不同间距灌丛引入对土壤细菌碳源利用和胞外酶活性的影响

苏荣霞(), 马彦平, 王红梅(), 赵亚楠, 李志丽   

  1. 宁夏大学农学院,宁夏草牧业工程技术研究中心,西北退化生态系统恢复与重建教育部重点培育实验室,宁夏 银川 750021
  • 收稿日期:2023-01-06 修回日期:2023-03-06 出版日期:2023-11-20 发布日期:2023-09-27
  • 通讯作者: 王红梅
  • 作者简介:E-mail: whm_826@163.com
    苏荣霞(1997-),女,宁夏固原人,在读硕士。E-mail: 1303338611@qq.com
  • 基金资助:
    国家自然科学基金项目(31860677);宁夏高等学校一流学科建设项目(NXYLXK2017A01)

Changes in soil bacterial carbon source utilization and soil extracellular enzyme activity after artificial planting of Caragana korshinskii at different densities on the desert steppe

Rong-xia SU(), Yan-ping MA, Hong-mei WANG(), Ya-nan ZHAO, Zhi-li LI   

  1. School of Agriculture,Ningxia University,Ningxia Grassland and Animal Husbandry Engineering Technology Research Center,Key Cultivation Laboratory of Ecosystem Restoration and Reconstruction,Ministry of Education,Yinchuan 750021,China
  • Received:2023-01-06 Revised:2023-03-06 Online:2023-11-20 Published:2023-09-27
  • Contact: Hong-mei WANG

摘要:

为探究不同间距柠条锦鸡儿人工灌丛引入对宁夏东部荒漠草原土壤细菌群落功能多样性和土壤胞外酶活性的影响,利用Biolog-ECO法对封育草地(17 a)和不同间距 (40、6和2 m) 灌丛地土壤细菌碳源利用特征展开研究。结果表明:荒漠草原不同间距灌丛引入后土壤有机碳和微生物生物量碳较封育草地无显著增加,而土壤全碳显著增加26.65%~43.47%;除β-1,4-N-乙酰基氨基葡萄糖苷酶外的其他土壤胞外酶活性均在灌丛地低于封育草地,灌丛间无显著差异。各样地土壤微生物细菌平均颜色变化率和主成分分析显示出近30年不同间距灌丛地土壤细菌群落代谢功能明显变化,灌丛密度增加,碳源的相对利用率下降了49.2%,但灌丛地碳源利用率仍显著大于封育草地,特别是土壤细菌群落功能多样性。丰富度、均匀度、优势度指数随密度增加而增大,碳源利用类型也从草原以氨基酸类、聚合物类和其他类碳源为主利用转向随灌丛以聚合物类和氨基酸类稳定优先利用为主。

关键词: 荒漠草原, 灌丛引入, 不同密度, 土壤细菌, 碳源利用

Abstract:

The aim of this study was to explore the effects of shrub introduction on the functional diversity of soil bacterial communities and soil extracellular enzyme activity on the desert steppe. Field studies were conducted in areas where Caragana korshinskii has been introduced with different planting intervals into grassland on the desert steppe of eastern Ningxia. We investigated the characteristics of soil bacterial carbon utilization in fenced grassland and shrublands planted with C. korshinskii at a range of intervals (40, 6 and 2 m) using the Biolog-ECO method. We found that the soil organic carbon and microbial biomass carbon contents were not significantly higher in shrublands than in enclosed desert grassland, while soil total carbon content was significantly higher (by 26.65%-43.47%) in shrublands than in enclosed grassland. The activities of all measured soil extracellular enzymes, except β-1,4-N-acetylamino glucosidase, were lower in shrublands than in enclosed grassland, but there was no significant difference in their activities among the different planting intervals. Further analyses including a principal component analysis revealed significant differences in the metabolic functions of soil bacterial communities among sites. The relative utilization of carbon sources decreased by 49.2% with the increase of shrub introduction intervals in the last 30 years. The utilization of carbon sources by soil bacteria and the functional diversity of soil bacterial communities were significantly higher in shrublands than in enclosed grassland. The Shannon-Wiener richness index, Shannon’s evenness index, and Simpson’s dominance index of soil bacterial communities increased with increasing planting intervals of C. korshinskii. Soil bacteria in grassland mainly utilized amino acids, polymers, and other types of compounds as carbon sources, but those in shrublands stably utilized polymers and amino acids as carbon sources.

Key words: desert grassland, shrub introduction, different densities, soil bacteria, carbon source utilization