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草业学报 ›› 2025, Vol. 34 ›› Issue (6): 122-138.DOI: 10.11686/cyxb2024289

• 研究论文 • 上一篇    

补饲对放牧滩羊生长性能、血清生化及代谢组的影响

徐倩1,2,3(), 郭帅2,4, 张亮亮5, 王占军6, 辛国省1,2,3()   

  1. 1.宁夏大学生命科学学院,宁夏 银川 750021
    2.宁夏饲料工程技术研究中心,宁夏 银川 750021
    3.宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏 银川 750021
    4.宁夏大学动物科技学院,宁夏 银川 750021
    5.宁夏昊标检测服务研究院,宁夏 银川 750021
    6.宁夏农林科学院林业与草地生态研究所,宁夏 银川 750021
  • 收稿日期:2024-07-23 修回日期:2024-09-09 出版日期:2025-06-20 发布日期:2025-04-03
  • 通讯作者: 辛国省
  • 作者简介:Corresponding author. E-mail: gsxin@nxu.edu.cn
    徐倩(2001-),女,安徽阜阳人,在读硕士。E-mail: 3282401415@qq.com
  • 基金资助:
    宁夏回族自治区农业高质量发展和生态保护科技创新示范课题(NGSB-2021-15-05);宁夏重点研发计划项目(2023BCF01037)

Effects of supplemental feeding on growth rate, and serum biochemical and metabolomic parameters of grazing Tan sheep

Qian XU1,2,3(), Shuai GUO2,4, Liang-liang ZHANG5, Zhan-jun WANG6, Guo-sheng XIN1,2,3()   

  1. 1.School of Life Sciences,Ningxia University,Yinchuan 750021,China
    2.Ningxia Feed Engineering Technology Research Center,Yinchuan 750021,China
    3.Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan 750021,China
    4.School of Animal Science and Technology,Ningxia University,Yinchuan 750021,China
    5.Ningxia Haobiao Testing Service Research Institute,Yinchuan 750021,China
    6.Institute of Forestry and Grassland Ecology,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750021,China
  • Received:2024-07-23 Revised:2024-09-09 Online:2025-06-20 Published:2025-04-03
  • Contact: Guo-sheng XIN

摘要:

本试验旨在探究补饲水平对放牧滩羊生长性能、血液生化、抗氧化及血液代谢组的影响。选取体况一致、体重相近[(23.38±0.70) kg]的3月龄去势公滩羊24只,随机分为4组。NC组(对照组)只放牧,LE(低能量组)、ME(中能量组)、HE(高能量组)组(试验组)先补饲精料再放牧,各组补饲精料消化能水平分别为15.91(LE)、17.63(ME)和18.17(HE) MJ·kg-1。预试期12 d,正试期90 d。结果显示:1)与NC和LE组相比,HE、ME组滩羊的末重、平均日增重均显著增加(P<0.05)。2)ME组的经济效益高于其他组。3)滩羊血液中空腹血糖、总胆固醇、甘油三酯、肌酐含量,天门冬氨酸氨基转移酶活性和低密度脂蛋白胆固醇浓度随着饲粮能量水平的上升呈增加趋势,其中HE组的甘油三酯含量显著高于其他组(P<0.05)。4)能量水平可显著影响抗氧化能力,HE组丙二醛含量显著高于NC和LE组(P<0.05),ME组总抗氧化能力显著高于NC组(P<0.05),NC组谷胱甘肽过氧化物酶活性显著高于LE和ME组(P<0.05)。5)血浆代谢组学分析共鉴定出17种代谢物差异显著,富集的代谢通路主要有嘌呤代谢、核苷酸代谢、初级胆汁酸生物合成、花生四烯酸代谢、胆固醇代谢、酪氨酸代谢等。综上所述,提高能量水平可有效提升放牧滩羊的生长性能,这种改善可能是通过调节嘌呤代谢、花生四烯酸代谢等途径及其中间代谢产物来调控机体的抗氧化和免疫功能。在本试验条件下,精料补饲水平为0.45 kg·d-1时显示出较好的效果。

关键词: 能量, 人工草地, 宁夏滩羊, 放牧, 抗氧化, 血浆代谢

Abstract:

The aim of this experiment was to investigate the effects of supplemental feeding on growth rate, blood biochemistry, and antioxidant and blood metabolomic parameters of grazing Tan sheep. In the experiment, twenty-four 3-month-old castrated male Tan sheep, with similar body weight (23.38±0.70 kg), were randomly divided into four groups: a ‘no concentrate’ group (NC, control) which were grazed only, and low energy (LE), medium energy (ME), and high energy (HE) groups which were the experimental groups and received supplemental concentrate feed before grazing. The digestible energy content of supplemented concentrate was 15.91, 17.63 and 18.17 MJ·kg-1 for LE, ME and HE, respectively. There was a pre-trial adjustment period lasting 12 days and the experimental period lasted for 90 days. The results show: 1) Compared with NC and LE, the final weight and daily weight gain of the HE and ME groups was significantly increased (P<0.05). 2) The economic return of ME was higher than the economic returns from the other groups. 3) Fasting blood glucose, and levels of total cholesterol, triglycerides, creatinine, aspartate aminotransferase and low-density lipoprotein cholesterol concentrations in the blood of the sheep all tended to increase with increase in supplemental concentrate energy level, with triglyceride content in HE being notably higher than in the other groups (P<0.05). 4) The energy level markedly affected the animals’ antioxidant capacity; the serum malondialdehyde content in HE was significantly higher than in NC and LE (P<0.05). In addition, the total antioxidant capacity in ME was notably higher than the NC (P<0.05). Moreover, glutathione peroxidase activity in NC was notably higher than in LE and ME (P<0.05). 5) The plasma metabolomics analysis identified a total of 17 metabolites with significant differences. The enriched metabolic pathways were mainly purine metabolism, nucleotide metabolism, primary bile acid biosynthesis, arachidonic acid metabolism, cholesterol metabolism, and tyrosine metabolism. In summary, increasing energy level effectively improved the growth rate of grazing Tan sheep. Blood biochemistry and metabolomic data indicate this improvement may be through the regulation of purine metabolism, arachidonic acid metabolism and other pathways and their intermediate metabolites to enhance body antioxidant and immune functions. Under the conditions of this experiment, the supplemental concentrate level of 0.45 kg·animal-1·d-1 (ME) showed the best effect.

Key words: energy, artificial grassland, Ningxia Tan sheep, grazing, antioxidant, plasma metabolome