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草业学报 ›› 2019, Vol. 28 ›› Issue (10): 199-208.DOI: 10.11686/cyxb2018721

• 研究论文 • 上一篇    下一篇

不同品种紫花苜蓿转录组分析及营养品质差异的探讨

成启明1, 格根图1, 撒多文2, 王志军3, 范文强4, 卜振鲲1, 司强1, 李俊峰1, 卢娟5, 贾玉山1,*   

  1. 1.内蒙古农业大学草原与资源环境学院,农业部饲草栽培、加工与高效利用重点试验室,草地资源教育部重点实验室,内蒙古 呼和浩特 010011;
    2.内蒙古自治区科技信息研究院,内蒙古 呼和浩特 010010;
    3.内蒙古自治区草原勘察规划院,内蒙古 呼和浩特 010051;
    4.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    5.四川省会东县农牧局,四川 凉山 615000
  • 收稿日期:2018-10-30 修回日期:2018-12-10 出版日期:2019-10-20 发布日期:2019-10-20
  • 通讯作者: E-mail: jys_nm@sina.com
  • 作者简介:成启明(1990-),男,四川广安人,在读博士。E-mail: 429845801@qq.com
  • 基金资助:
    国家自然基金面上项目(31572461)和国家重点研发项目(2016YFC0501308-3)资助

Transcriptome analyses provide insights into differences in nutritional quality among different alfalfa varieties

CHENG Qi-ming1, GE Gen-tu1, SA Duo-wen2, WANG Zhi-jun3, FAN Wen-qiang4, BU Zhen-kun1, SI Qiang1, LI Jun-feng1, LU Juan5, JIA Yu-shan1,*   

  1. 1.College of Grassland, Resources and Environment, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of the Ministry of Agriculture, and Key Laboratory of Grassland Resources of the Ministry of Education P.R of China, Inner Mongolia Agricultural University, Hohhot 010011, China;
    2.Inner Mongolia Autonomous Region of institute of scientific & Technical information, Hohhot 010010, China;
    3.Inner Mongolia Grassland and Planning Institute, Hohhot 010051, China;
    4.Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China;
    5.Huidong County Agriculture and Animal Husbandry Bureau, Liangshan 615000, China
  • Received:2018-10-30 Revised:2018-12-10 Online:2019-10-20 Published:2019-10-20
  • Contact: E-mail: jys_nm@sina.com

摘要: 为了丰富紫花苜蓿转录组数据信息,找出不同品种紫花苜蓿的差异基因及代谢通路。通过Illumina HiSeq 4000平台对准格尔和WL319HQ的苜蓿叶片的RNA文库进行de novo组装。得到了约39G总的核苷酸,2亿多个reads,组装得到66734个Unigenes,平均长度为869 bp。将所得到的Unigenes与NCBI nonredundant protein(Nr),A manually annotated and reviewed protein sequence database(Swissprot),Clusters of eukaryotic orthologous groups(KOG)和Kyoto encyclopedia of genes and genomes(KEGG)数据库比对,分别获得44888(67.26%),29190(43.74%),24844(37.23%)和15647(23.45%)条序列的注释信息。在2个紫花苜蓿叶片中找到1098个差异表达基因 (DEGs) ,其中有706个上调,392个下调(准格尔-vs-WL319HQ)。对其差异基因做了Gene ontology(GO)功能分析和KEGG途径分析,初步分析了2个品种营养品质差异的内在原因。极大地丰富了紫花苜蓿转录组数据信息,为今后紫花苜蓿的转录组测序提供理论依据,同时为紫花苜蓿生产实践提供参考。

关键词: 紫花苜蓿, 转录组测序, 营养品质, de novo组装, 差异基因

Abstract: To enrich alfalfa (Medicago sativa) transcriptome data and clarify which genes and pathways are related to differences in nutritional quality among cultivars, two alfalfa varieties (Zhungeer and WL319HQ) were selected for de novo sequencing using the Illumina HiSeq 4000 platform. We obtained about 39 Gb total nucleotides and more than 200 million reads, and assembled 66734 unigenes with an average length of 869 bp. Then, 44888 (67.26%), 29190 (43.74%), 24844 (37.23%), and 15647 (23.45%) unigenes were annotated on the basis of information from the NCBI nonredundant protein (Nr), Swissprot (a manually annotated and reviewed protein sequence database), clusters of euKaryotic Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. We identified 1098 unigenes as differentially expressed genes (DEGs) in leaves between the two varieties of alfalfa (706 unigenes up-regulated and 392 unigenes down-regulated in Zhungeer vs. WL319HQ). The DEGs were subjected to Gene Ontology (GO) functional analysis and KEGG pathway enrichment analysis, the results of which preliminarily identified the intrinsic reasons for differences in nutritional quality between the two varieties. The data obtained in this study dramatically enrich the transcriptome data available for alfalfa. In addition, these data provide theoretical guidance for transcriptome sequencing of alfalfa in the future, as well as a reference for production practices.

Key words: Medicago sativa, transcriptome, nutritional quality, de novo, differentially expressed genes