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草业学报 ›› 2020, Vol. 29 ›› Issue (8): 155-169.DOI: 10.11686/cyxb2019486

• 研究论文 • 上一篇    下一篇

新疆不同生境狗牙根种质抗旱性综合评价

曾令霜1, 李培英1, 2, *, 孙晓梵1, 孙宗玖1, 2, *   

  1. 1. 新疆农业大学草业与环境科学学院,新疆 乌鲁木齐 830052;
    2. 新疆草地资源与生态重点实验室,新疆 乌鲁木齐 830052
  • 收稿日期:2019-11-13 修回日期:2019-12-24 出版日期:2020-08-20 发布日期:2020-08-20
  • 通讯作者: *. E-mail: nmlpy_1234@sina.com, nmszj@21cn.com
  • 作者简介:曾令霜(1995-),女,重庆永川人,在读硕士。E-mail: 1397705683@qq.com
  • 基金资助:
    国家自然科学基金项目(31960362)资助

A multi-trait evaluation of drought resistance of bermudagrass (Cynodon dactylon) germplasm from different habitats in Xinjiang province

ZENG Ling-shuang1, LI Pei-ying1, 2, *, SUN Xiao-fan1, SUN Zong-jiu1, 2, *   

  1. 1. Department of Pratacultural and Environmental Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi 830052, China
  • Received:2019-11-13 Revised:2019-12-24 Online:2020-08-20 Published:2020-08-20

摘要: 以2份国外品种(对照)和41份来自不同生境的新疆狗牙根种质为供试材料,设置干旱处理和对照处理,在30 d干旱处理后测定叶绿素含量、绿叶率、相对电导率、叶片相对含水量、脯氨酸含量、丙二醛含量、生长速度和地上生物量的基础上,采用相关分析、主成分分析构建综合评价指标,进一步结合隶属函数分析、聚类分析、灰色关联度分析及判别分析方法对其抗旱性进行综合评价,筛选抗旱鉴定关键指标,明确种质抗旱性与其生境气候带间关系。结果表明,干旱胁迫后供试种质的相对电导率、脯氨酸及丙二醛含量呈显著增加,叶片相对含水量、叶绿素含量、地上生物量、生长速度和绿叶率显著降低,各指标对抗旱性的响应存在一定差异。主成分分析将8个指标转换为6个综合指标,代表种质抗旱性87.34%的数据信息;依据抗旱性度量值(D)、综合抗旱系数(CDC)、加权抗旱系数(WDC)的隶属函数分析及聚类分析结果,认为C118、C138、C24抗旱性较强,C10、C134、C32抗旱性较弱。灰色关联度分析认为相对电导率、叶绿素含量及丙二醛含量可作为狗牙根种质抗旱评价的关键指标,且判别分析认为新疆狗牙根种质抗旱性与其生境气候带间无明显相关性。综合分析发现C118、C138、C24等种质抗旱性优于Tifway,其为狗牙根抗旱新品种选育提供可用优异种质,也为进一步研究狗牙根抗旱生理及分子机制提供可用材料。

关键词: 干旱胁迫, 狗牙根, 抗旱指标, 综合评价

Abstract: This research used two commercial varieties of bermudagrass (Cynodon dactylon) as control and evaluated performance of 41 germplasm accessions of bermudagrass collected from different habitats in Xinjiang province under drought and control treatments. After 30 days of drought treatment, the chlorophyll content, green leaf proportion, relative conductivity, leaf relative water content, proline content, malondialdehyde content, speed of growth and aboveground biomass under natural drought stress were measured. Based on these data, correlation analysis and principal component analysis were performed to construct multi-trait evaluation indicators, and combined with membership function analysis, cluster analysis, grey correlation analysis and discriminant analysis methods to comprehensively evaluate multiple trait expression linked to drought resistance, develop key indexes for identification of drought resistance, and clarify the relationship between drought resistance of germplasm accessions and their habitat climate zone. It was found that the relative conductivity, proline and malondialdehyde content of the tested germplasm significantly increased under drought stress, and the relative water content, chlorophyll content, aboveground biomass, speed of growth and green leaf rate significantly decreased. There were differences in response to drought stress between all indices. The eight measured traits were reduced by principal component analysis to six independent scores, representing 87.34% of the total data variation. The result of membership function analysis based on drought resistance comprehensive evaluation value (D), comprehensive drought resistance coefficient (CDC), and weighted drought resistance coefficient (WDC) analysis and cluster analysis showed that accessions designated C118, C138 and C24 had strong drought resistance, while accessions designated C10, C134 and C32 had weaker drought resistance. The grey correlation analysis indicated that relative conductivity, leaf chlorophyll content and malondialdehyde content could be used as key indexes for drought resistance evaluation of bermudagrass germplasm, and discriminant analysis indicated that there was no significant correlation between drought resistance of Xinjiang Bermudagrass germplasm and its habitat climate zone. The comprehensive analysis showed that the drought resistance of C118, C138, C24 and other germplasm acessions was better than that of the commercial variety Tifway. Hence this research has provided excellent germplasm for breeding new bermudagrass varieties with good drought resistance, and provided materials for further study of physiological and molecular mechanisms of bermudagrass drought resistance.

Key words: drought stress, Cynodon dactylon, drought resistance index, comprehensive evaluation