草业学报 ›› 2022, Vol. 31 ›› Issue (11): 181-190.DOI: 10.11686/cyxb2021459
李娜娜1(), 刘同歌1, 黄志慧1, 郑宝江2, 张玉红1()
收稿日期:
2021-12-10
修回日期:
2022-03-09
出版日期:
2022-11-20
发布日期:
2022-10-01
通讯作者:
张玉红
作者简介:
E-mail: pzhangyh@126.com基金资助:
Na-na LI1(), Tong-ge LIU1, Zhi-hui HUANG1, Bao-jiang ZHENG2, Yu-hong ZHANG1()
Received:
2021-12-10
Revised:
2022-03-09
Online:
2022-11-20
Published:
2022-10-01
Contact:
Yu-hong ZHANG
摘要:
旨在探究盐胁迫下菥蓂生理生态及次生代谢产物的响应,为进一步研究在盐碱地上耐盐植株的种植奠定理论基础。以菥蓂为试验材料,采用水培的方式进行培养,设置5个NaCl浓度梯度(0、50、100、150、200 mmol·L-1)来模拟不同强度盐胁迫条件,分别在处理1、5、10、15 d采集菥蓂叶片,测定其生理生化指标,光合参数和次生代谢产物含量。结果表明:菥蓂叶片中SOD、POD、CAT活性在盐胁迫1~15 d时均随盐浓度的升高呈先增后减的趋势,均在NaCl浓度为100 mmol·L-1时,酶活性达到最高。丙二醛含量随盐浓度的升高先增后减;菥蓂叶片可溶性糖和脯氨酸含量随盐浓度的升高和胁迫时间的延长而增加,可溶性蛋白含量随盐浓度的升高先增后减;菥蓂叶片中叶绿素a、叶绿素b、总叶绿素含量、叶绿素荧光参数、净光合速率和蒸腾速率随盐浓度的升高基本呈下降趋势,下降幅度逐渐增大;菥蓂叶片中总黄铜、总酚、黑芥子苷的含量随盐浓度的升高呈先增后减的趋势。由此可见,菥蓂对盐胁迫有较高的耐受性,在50~100 mmol·L-1盐胁迫环境中能正常生长,该结果可为后续菥蓂耐盐机制的研究提供理论依据。
李娜娜, 刘同歌, 黄志慧, 郑宝江, 张玉红. 草本资源植物菥蓂对盐胁迫下生理生态及次生代谢产物响应[J]. 草业学报, 2022, 31(11): 181-190.
Na-na LI, Tong-ge LIU, Zhi-hui HUANG, Bao-jiang ZHENG, Yu-hong ZHANG. Physiological ecological and secondary metabolic responses of the herbaceous resource plant Thlaspi arvense to salt stress[J]. Acta Prataculturae Sinica, 2022, 31(11): 181-190.
图2 NaCl胁迫对菥蓂叶片抗氧化酶活性和MDA含量的影响不同小写字母表示处理间差异显著(P<0.05),下同。Different lowercase letters indicate significant difference among different treatments at P<0.05, the same below.
Fig.2 Effect of NaCl stress on antioxidant enzyme activity and MDA content of T. arvense
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