草业学报 ›› 2025, Vol. 34 ›› Issue (6): 122-138.DOI: 10.11686/cyxb2024289
• 研究论文 • 上一篇
徐倩1,2,3(
), 郭帅2,4, 张亮亮5, 王占军6, 辛国省1,2,3(
)
收稿日期:2024-07-23
修回日期:2024-09-09
出版日期:2025-06-20
发布日期:2025-04-03
通讯作者:
辛国省
作者简介:Corresponding author. E-mail: gsxin@nxu.edu.cn基金资助:
Qian XU1,2,3(
), Shuai GUO2,4, Liang-liang ZHANG5, Zhan-jun WANG6, Guo-sheng XIN1,2,3(
)
Received:2024-07-23
Revised:2024-09-09
Online:2025-06-20
Published:2025-04-03
Contact:
Guo-sheng XIN
摘要:
本试验旨在探究补饲水平对放牧滩羊生长性能、血液生化、抗氧化及血液代谢组的影响。选取体况一致、体重相近[(23.38±0.70) kg]的3月龄去势公滩羊24只,随机分为4组。NC组(对照组)只放牧,LE(低能量组)、ME(中能量组)、HE(高能量组)组(试验组)先补饲精料再放牧,各组补饲精料消化能水平分别为15.91(LE)、17.63(ME)和18.17(HE) MJ·kg-1。预试期12 d,正试期90 d。结果显示:1)与NC和LE组相比,HE、ME组滩羊的末重、平均日增重均显著增加(P<0.05)。2)ME组的经济效益高于其他组。3)滩羊血液中空腹血糖、总胆固醇、甘油三酯、肌酐含量,天门冬氨酸氨基转移酶活性和低密度脂蛋白胆固醇浓度随着饲粮能量水平的上升呈增加趋势,其中HE组的甘油三酯含量显著高于其他组(P<0.05)。4)能量水平可显著影响抗氧化能力,HE组丙二醛含量显著高于NC和LE组(P<0.05),ME组总抗氧化能力显著高于NC组(P<0.05),NC组谷胱甘肽过氧化物酶活性显著高于LE和ME组(P<0.05)。5)血浆代谢组学分析共鉴定出17种代谢物差异显著,富集的代谢通路主要有嘌呤代谢、核苷酸代谢、初级胆汁酸生物合成、花生四烯酸代谢、胆固醇代谢、酪氨酸代谢等。综上所述,提高能量水平可有效提升放牧滩羊的生长性能,这种改善可能是通过调节嘌呤代谢、花生四烯酸代谢等途径及其中间代谢产物来调控机体的抗氧化和免疫功能。在本试验条件下,精料补饲水平为0.45 kg·d-1时显示出较好的效果。
徐倩, 郭帅, 张亮亮, 王占军, 辛国省. 补饲对放牧滩羊生长性能、血清生化及代谢组的影响[J]. 草业学报, 2025, 34(6): 122-138.
Qian XU, Shuai GUO, Liang-liang ZHANG, Zhan-jun WANG, Guo-sheng XIN. Effects of supplemental feeding on growth rate, and serum biochemical and metabolomic parameters of grazing Tan sheep[J]. Acta Prataculturae Sinica, 2025, 34(6): 122-138.
| 项目Item | 值Value |
|---|---|
| 粗蛋白质 Crude protein (CP,%) | 12.89 |
| 酸性洗涤纤维 Acid detergent fiber (ADF,%) | 39.28 |
| 中性洗涤纤维 Neutral detergent fiber (NDF,%) | 53.54 |
| 钙 Ca (%) | 1.05 |
| 磷 P (%) | 0.18 |
| 消化能 Digestible energy (DE,MJ·kg-1) | 10.64 |
表1 牧草营养水平(干物质基础)
Table 1 Nutrient levels of grazed grass forage(dry matter basis)
| 项目Item | 值Value |
|---|---|
| 粗蛋白质 Crude protein (CP,%) | 12.89 |
| 酸性洗涤纤维 Acid detergent fiber (ADF,%) | 39.28 |
| 中性洗涤纤维 Neutral detergent fiber (NDF,%) | 53.54 |
| 钙 Ca (%) | 1.05 |
| 磷 P (%) | 0.18 |
| 消化能 Digestible energy (DE,MJ·kg-1) | 10.64 |
项目 Items | 组别 Groups | ||
|---|---|---|---|
| LE | ME | HE | |
| 原料 Ingredients | |||
| 玉米 Corn (%) | 79.27 | 71.53 | 72.62 |
| 酒糟 | 0.67 | 11.73 | 12.02 |
| 磷酸氢钙 CaHPO4 (%) | 6.00 | 3.00 | 2.00 |
| 大豆油 Soybean oil (%) | 4.20 | 7.83 | 8.56 |
| 尿素 Urea (%) | 0.00 | 0.97 | 1.51 |
| 小苏打 NaHCO3 (%) | 1.33 | 0.67 | 0.45 |
| 食用盐 NaCl (%) | 5.20 | 2.60 | 1.73 |
| 预混料 Premix1) (%) | 3.33 | 1.67 | 1.11 |
| 合计 Total (%) | 100 | 100 | 100 |
| 营养水平 Nutrient level2) | |||
| 粗蛋白质 Crude protein (CP,%) | 7.11 | 12.43 | 14.22 |
| 酸性洗涤纤维 Acid detergent fiber (ADF,%) | 2.69 | 3.41 | 3.47 |
| 中性洗涤纤维 Neutral detergent fiber (NDF,%) | 1.19 | 2.46 | 2.51 |
| 钙 Ca (%) | 2.08 | 1.09 | 0.76 |
| 磷 P (%) | 1.70 | 1.03 | 0.78 |
| 消化能 Digestible energy (DE,MJ·kg-1) | 15.91 | 17.63 | 18.17 |
表2 精料补充料组成及营养水平(干物质基础)
Table 2 The composition and nutrient levels of concentrates diet (dry matter basis)
项目 Items | 组别 Groups | ||
|---|---|---|---|
| LE | ME | HE | |
| 原料 Ingredients | |||
| 玉米 Corn (%) | 79.27 | 71.53 | 72.62 |
| 酒糟 | 0.67 | 11.73 | 12.02 |
| 磷酸氢钙 CaHPO4 (%) | 6.00 | 3.00 | 2.00 |
| 大豆油 Soybean oil (%) | 4.20 | 7.83 | 8.56 |
| 尿素 Urea (%) | 0.00 | 0.97 | 1.51 |
| 小苏打 NaHCO3 (%) | 1.33 | 0.67 | 0.45 |
| 食用盐 NaCl (%) | 5.20 | 2.60 | 1.73 |
| 预混料 Premix1) (%) | 3.33 | 1.67 | 1.11 |
| 合计 Total (%) | 100 | 100 | 100 |
| 营养水平 Nutrient level2) | |||
| 粗蛋白质 Crude protein (CP,%) | 7.11 | 12.43 | 14.22 |
| 酸性洗涤纤维 Acid detergent fiber (ADF,%) | 2.69 | 3.41 | 3.47 |
| 中性洗涤纤维 Neutral detergent fiber (NDF,%) | 1.19 | 2.46 | 2.51 |
| 钙 Ca (%) | 2.08 | 1.09 | 0.76 |
| 磷 P (%) | 1.70 | 1.03 | 0.78 |
| 消化能 Digestible energy (DE,MJ·kg-1) | 15.91 | 17.63 | 18.17 |
| 项目 Items | 组别Groups | 标准误 Standard error (SEM) | P值 P-value | |||
|---|---|---|---|---|---|---|
| NC | LE | ME | HE | |||
| 初重 Initial weight (IW, kg) | 23.60 | 23.60 | 23.65 | 24.04 | 0.18 | 0.792 |
| 末重 Final weight (FW, kg) | 33.98b | 34.15b | 40.35a | 42.00a | 1.01 | <0.001 |
| 平均日增重 Average daily gain (ADG, g·d-1) | 102.70b | 105.39b | 163.73a | 177.70a | 9.31 | <0.001 |
表3 补饲对放牧滩羊生长性能的影响
Table 3 Effects of supplemental feeding on growth performance of grazing Tan sheep
| 项目 Items | 组别Groups | 标准误 Standard error (SEM) | P值 P-value | |||
|---|---|---|---|---|---|---|
| NC | LE | ME | HE | |||
| 初重 Initial weight (IW, kg) | 23.60 | 23.60 | 23.65 | 24.04 | 0.18 | 0.792 |
| 末重 Final weight (FW, kg) | 33.98b | 34.15b | 40.35a | 42.00a | 1.01 | <0.001 |
| 平均日增重 Average daily gain (ADG, g·d-1) | 102.70b | 105.39b | 163.73a | 177.70a | 9.31 | <0.001 |
项目 Items | 组别Groups | |||
|---|---|---|---|---|
| NC | LE | ME | HE | |
| 牧草干物质采食量Forage dry matter intake (kg·piece-1·d-1) | 1.50 | 1.28 | 1.05 | 0.83 |
| 牧草干草价格Forage hay price (CNY·kg-1) | 0.94 | 0.94 | 0.94 | 0.94 |
| 牧草成本Forage cost (CNY·piece-1·d-1) | 1.41 | 1.20 | 0.99 | 0.78 |
| 补饲精料单价Concentrate supplementation price (CNY·kg-1) | - | 3.52 | 3.86 | 3.95 |
| 补饲量 Feeding levels of concentrate supplementation (kg·piece-1·d-1) | - | 0.23 | 0.45 | 0.68 |
| 精料补饲成本Concentrate supplementation cost (CNY·piece-1·d-1) | - | 0.79 | 1.74 | 2.67 |
| 滩羊活重价格Lamb sale price (CNY·kg-1) | 36.00 | 36.00 | 36.00 | 36.00 |
| 平均日增重Average daily gain (g·piece-1·d-1) | 102.70 | 105.39 | 163.73 | 177.70 |
| 增重收益Weight gain profits (CNY·piece-1·d-1) | 3.70 | 3.79 | 5.89 | 6.40 |
| 经济效益Economic benefits (CNY·piece-1·d-1) | 2.29 | 1.80 | 3.17 | 2.96 |
表4 补饲对放牧滩羊经济效益的影响
Table 4 Effects of supplemental feeding on economic benefits of grazing Tan sheep
项目 Items | 组别Groups | |||
|---|---|---|---|---|
| NC | LE | ME | HE | |
| 牧草干物质采食量Forage dry matter intake (kg·piece-1·d-1) | 1.50 | 1.28 | 1.05 | 0.83 |
| 牧草干草价格Forage hay price (CNY·kg-1) | 0.94 | 0.94 | 0.94 | 0.94 |
| 牧草成本Forage cost (CNY·piece-1·d-1) | 1.41 | 1.20 | 0.99 | 0.78 |
| 补饲精料单价Concentrate supplementation price (CNY·kg-1) | - | 3.52 | 3.86 | 3.95 |
| 补饲量 Feeding levels of concentrate supplementation (kg·piece-1·d-1) | - | 0.23 | 0.45 | 0.68 |
| 精料补饲成本Concentrate supplementation cost (CNY·piece-1·d-1) | - | 0.79 | 1.74 | 2.67 |
| 滩羊活重价格Lamb sale price (CNY·kg-1) | 36.00 | 36.00 | 36.00 | 36.00 |
| 平均日增重Average daily gain (g·piece-1·d-1) | 102.70 | 105.39 | 163.73 | 177.70 |
| 增重收益Weight gain profits (CNY·piece-1·d-1) | 3.70 | 3.79 | 5.89 | 6.40 |
| 经济效益Economic benefits (CNY·piece-1·d-1) | 2.29 | 1.80 | 3.17 | 2.96 |
项目 Items | 组别Groups | 标准误Standard error (SEM) | P值 P-value | |||
|---|---|---|---|---|---|---|
| NC | LE | ME | HE | |||
| 总蛋白Total protein (TP,g·L-1) | 74.20 | 71.50 | 74.94 | 72.93 | 0.92 | 0.602 |
| 白蛋白Albumin (ALB,g·L-1) | 32.63ab | 30.53b | 33.75a | 33.40a | 0.46 | 0.029 |
| 球蛋白Globulin (GLB,g·L-1) | 40.18 | 40.15 | 43.53 | 41.80 | 0.85 | 0.489 |
| 总胆固醇Total cholesterol (TC,mmol·L-1) | 1.60b | 1.90b | 2.48a | 2.66a | 0.13 | <0.001 |
| 甘油三酯Triglyceride (TG,mmol·L-1) | 0.50b | 0.55b | 0.53b | 0.69a | 0.02 | 0.032 |
| 空腹血糖Glucose (GLU,mmol·L-1) | 2.87b | 3.26b | 4.02a | 4.12a | 0.16 | 0.002 |
| 肌酐Creatinine (CREA,μmol·L-1) | 61.40c | 67.00bc | 73.25b | 88.00a | 2.56 | <0.001 |
| 天门冬氨酸氨基转移酶Aspartate aminotransferase (AST,U·L-1) | 117.50b | 119.75b | 131.25ab | 150.00a | 4.71 | 0.036 |
| 丙氨酸氨基转移酶Alanine aminotransferase (ALT,U·L-1) | 21.75 | 18.75 | 18.75 | 18.25 | 0.69 | 0.278 |
| 乳酸脱氢酶Lactate dehydrogenase (LDH,U·L-1) | 460.25bc | 403.67c | 496.00b | 567.67a | 17.79 | 0.002 |
| 高密度脂蛋白胆固醇High density lipoprotein cholesterol (HDL,mmol·L-1) | 0.75c | 0.95bc | 1.19a | 1.07ab | 0.05 | 0.005 |
| 低密度脂蛋白胆固醇Low density lipoprotein cholesterol (LDL,mmol·L-1) | 0.33c | 0.39bc | 0.55ab | 0.71a | 0.05 | 0.002 |
表5 补饲对放牧滩羊血清生化指标的影响
Table 5 Effects of supplemental feeding on serum biochemical indexes of grazing Tan sheep
项目 Items | 组别Groups | 标准误Standard error (SEM) | P值 P-value | |||
|---|---|---|---|---|---|---|
| NC | LE | ME | HE | |||
| 总蛋白Total protein (TP,g·L-1) | 74.20 | 71.50 | 74.94 | 72.93 | 0.92 | 0.602 |
| 白蛋白Albumin (ALB,g·L-1) | 32.63ab | 30.53b | 33.75a | 33.40a | 0.46 | 0.029 |
| 球蛋白Globulin (GLB,g·L-1) | 40.18 | 40.15 | 43.53 | 41.80 | 0.85 | 0.489 |
| 总胆固醇Total cholesterol (TC,mmol·L-1) | 1.60b | 1.90b | 2.48a | 2.66a | 0.13 | <0.001 |
| 甘油三酯Triglyceride (TG,mmol·L-1) | 0.50b | 0.55b | 0.53b | 0.69a | 0.02 | 0.032 |
| 空腹血糖Glucose (GLU,mmol·L-1) | 2.87b | 3.26b | 4.02a | 4.12a | 0.16 | 0.002 |
| 肌酐Creatinine (CREA,μmol·L-1) | 61.40c | 67.00bc | 73.25b | 88.00a | 2.56 | <0.001 |
| 天门冬氨酸氨基转移酶Aspartate aminotransferase (AST,U·L-1) | 117.50b | 119.75b | 131.25ab | 150.00a | 4.71 | 0.036 |
| 丙氨酸氨基转移酶Alanine aminotransferase (ALT,U·L-1) | 21.75 | 18.75 | 18.75 | 18.25 | 0.69 | 0.278 |
| 乳酸脱氢酶Lactate dehydrogenase (LDH,U·L-1) | 460.25bc | 403.67c | 496.00b | 567.67a | 17.79 | 0.002 |
| 高密度脂蛋白胆固醇High density lipoprotein cholesterol (HDL,mmol·L-1) | 0.75c | 0.95bc | 1.19a | 1.07ab | 0.05 | 0.005 |
| 低密度脂蛋白胆固醇Low density lipoprotein cholesterol (LDL,mmol·L-1) | 0.33c | 0.39bc | 0.55ab | 0.71a | 0.05 | 0.002 |
项目 Items | 组别Groups | 标准误Standard error (SEM) | P值 P-value | |||
|---|---|---|---|---|---|---|
| NC | LE | ME | HE | |||
| 丙二醛Malondialdehyde (MDA,nmol·mL-1) | 1.04b | 0.77b | 1.16ab | 1.65a | 0.11 | 0.036 |
| 过氧化氢酶Catalase (CAT,U·mL-1) | 3.14 | 3.51 | 3.58 | 3.96 | 0.15 | 0.231 |
| 超氧化物歧化酶Superoxide dismutase (SOD,U·mL-1) | 50.40b | 53.38b | 56.83ab | 60.46a | 1.39 | 0.038 |
| 总抗氧化能力Total antioxidant capacity (T-AOC,U·mL-1) | 2.56b | 2.90ab | 4.26a | 3.66ab | 0.26 | 0.046 |
| 谷胱甘肽过氧化物酶 Glutathione peroxidase (GSH-Px,U·mL-1) | 499.47a | 176.42b | 185.96b | 371.92ab | 43.82 | 0.011 |
表6 补饲对放牧滩羊抗氧化指标的影响
Table 6 Effects of supplemental feeding on antioxidant indexes of grazing Tan sheep
项目 Items | 组别Groups | 标准误Standard error (SEM) | P值 P-value | |||
|---|---|---|---|---|---|---|
| NC | LE | ME | HE | |||
| 丙二醛Malondialdehyde (MDA,nmol·mL-1) | 1.04b | 0.77b | 1.16ab | 1.65a | 0.11 | 0.036 |
| 过氧化氢酶Catalase (CAT,U·mL-1) | 3.14 | 3.51 | 3.58 | 3.96 | 0.15 | 0.231 |
| 超氧化物歧化酶Superoxide dismutase (SOD,U·mL-1) | 50.40b | 53.38b | 56.83ab | 60.46a | 1.39 | 0.038 |
| 总抗氧化能力Total antioxidant capacity (T-AOC,U·mL-1) | 2.56b | 2.90ab | 4.26a | 3.66ab | 0.26 | 0.046 |
| 谷胱甘肽过氧化物酶 Glutathione peroxidase (GSH-Px,U·mL-1) | 499.47a | 176.42b | 185.96b | 371.92ab | 43.82 | 0.011 |
组别 Group | 代谢物 Metabolites | 差异倍数 Fold change (FC) | 变量重要性投影 Variable importance project (VIP) | 类型 Type | 通路号 Pathway ID |
|---|---|---|---|---|---|
NC组 vs LE组 NC group vs LE group | 腺苷-3'-磷酸Adenosine-3'-phosphate | 4.13 | 1.09 | 上调Up | ko00230、ko01100 |
| 5'-腺嘌呤核苷酸5'-adenosine monophosphate | 4.00 | 1.74 | 上调Up | ko00230、ko01100、ko01232、ko01240、ko01523、ko04022、ko04024、ko04068、ko04150、ko04151、ko04152、ko04211、ko04740、ko04742、ko04923、ko04924、ko04925、ko04927、ko04928、ko04934、ko05012、ko05022、ko05032 | |
2'-脱氧鸟苷5'-一磷酸 2'-deoxyguanosine 5'-monophosphate (dGMP) | 2.28 | 1.69 | 上调Up | ko00230、ko01100、ko01232 | |
| 鸟苷5'-单磷酸Guanosine-5'-monophosphate | 3.35 | 1.45 | 上调Up | ko00230、ko01100、ko01232、ko01523、ko04022、ko04740、ko04742、ko04744 | |
| 二十二碳六烯酸Docosahexaenoic acid (DHA) | 0.49 | 1.41 | 下调Down | ko01040 | |
NC组 vs ME组 NC group vs ME group | 5'-腺嘌呤核苷酸5'-adenosine monophosphate | 8.19 | 1.58 | 上调Up | ko00230、ko01100、ko01232、ko01240、ko01523、ko04022、ko04024、ko04068、ko04150、ko04151、ko04152、ko04211、ko04740、ko04742、ko04923、ko04924、ko04925、ko04927、ko04928、ko04934、ko05012、ko05022、ko05032 |
| 2'-脱氧鸟苷2'-deoxyguanosine | 2.01 | 1.45 | 上调Up | ko00230、ko01100、ko01232、ko02010 | |
2'-脱氧鸟苷5'-一磷酸 2'-deoxyguanosine 5'-monophosphate (dGMP) | 3.70 | 1.17 | 上调Up | ko00230、ko01100、ko01232、ko02010 | |
| 鸟苷5'-单磷酸Guanosine-5'-monophosphate | 4.64 | 1.35 | 上调Up | ko00230、ko01100、ko01232、ko01523、ko04022、ko04740、ko04742、ko04744 | |
| 肌苷5'-单磷酸Inosine 5'-monophosphate | 3.62 | 1.39 | 上调Up | ko00230、ko01100、ko01232、ko01240、ko01523、ko04742 | |
| 二十二碳六烯酸Docosahexaenoic acid (DHA) | 0.48 | 1.38 | 下调Down | ko01040 | |
| NC组 vs HE组NC group vs HE group | 2,5-二羟基苯甲酸2,5-dihydroxybenzoic acid | 0.48 | 2.12 | 下调Down | ko00350、ko01100 |
| 3-羟基苯乙酸3-hydroxyphenylacetic acid | 2.39 | 1.83 | 上调Up | ko00350、ko00360、ko01100 | |
| 二十二碳六烯酸Docosahexaenoic acid (DHA) | 0.38 | 1.74 | 下调Down | ko01040 | |
ME组 vs LE组 ME group vs LE group | 鹅去氧胆酸Chenodeoxycholic acid | 0.20 | 1.56 | 下调Down | ko00120、ko01100、ko04976 |
| 脱氧胆酸Deoxycholic acid | 0.20 | 1.64 | 下调Down | ko04976 | |
| 2'-脱氧鸟苷2'-deoxyguanosine | 0.48 | 1.60 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 次黄嘌呤Hypoxanthine | 0.50 | 1.70 | 下调Down | ko00230、ko01100、ko01232 | |
| 肌苷Inosine | 0.46 | 1.89 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 牛磺鹅去氧胆酸Taurochenodesoxycholic acid | 0.33 | 2.01 | 下调Down | ko00120、ko04976、ko04979 | |
HE组 vs LE组 HE group vs LE group | 2'-脱氧肌苷2'-deoxyinosine | 0.43 | 2.03 | 下调Down | ko00230、ko01100、ko01232、ko02010 |
| 2'-脱氧鸟苷2'-deoxyguanosine | 0.45 | 1.47 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 甘氨鹅脱氧胆酸Glycochenodeoxycholic acid | 3.44 | 1.58 | 上调Up | ko00120、ko04976、ko04979 | |
| 鸟苷Guanosine | 0.34 | 1.17 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 次黄嘌呤Hypoxanthine | 0.48 | 1.27 | 下调Down | ko00230、ko01100、ko01232 | |
| 肌苷Inosine | 0.39 | 1.29 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 牛磺鹅去氧胆酸Taurochenodesoxycholic acid | 0.20 | 1.38 | 下调Down | ko00120、ko04976、ko04979 | |
HE组 vs ME组 HE group vs ME group | 11,12-双羟基-5Z,8Z,14Z-二十二碳三烯酸 11,12-dihydroxy-5Z,8Z,14Z-docosatrienoic acid | 3.18 | 1.07 | 上调Up | ko00590、ko01100、ko04726 |
8,9-双羟基-5Z,11Z,14Z-二十二碳三烯酸 8,9-dihydroxy-5Z,11Z,14Z-docosatrienoic acid | 3.18 | 1.07 | 上调Up | ko00590、ko01100、ko04726 | |
15-脱氧-δ-12,14-前列腺素J2 15-deoxy-δ-12,14-prostaglandin J2 | 4.58 | 1.27 | 上调Up | ko00590 |
表7 差异代谢物KEGG代谢通路富集分析
Table 7 Enrichment analysis of KEGG metabolic pathway of differential metabolites
组别 Group | 代谢物 Metabolites | 差异倍数 Fold change (FC) | 变量重要性投影 Variable importance project (VIP) | 类型 Type | 通路号 Pathway ID |
|---|---|---|---|---|---|
NC组 vs LE组 NC group vs LE group | 腺苷-3'-磷酸Adenosine-3'-phosphate | 4.13 | 1.09 | 上调Up | ko00230、ko01100 |
| 5'-腺嘌呤核苷酸5'-adenosine monophosphate | 4.00 | 1.74 | 上调Up | ko00230、ko01100、ko01232、ko01240、ko01523、ko04022、ko04024、ko04068、ko04150、ko04151、ko04152、ko04211、ko04740、ko04742、ko04923、ko04924、ko04925、ko04927、ko04928、ko04934、ko05012、ko05022、ko05032 | |
2'-脱氧鸟苷5'-一磷酸 2'-deoxyguanosine 5'-monophosphate (dGMP) | 2.28 | 1.69 | 上调Up | ko00230、ko01100、ko01232 | |
| 鸟苷5'-单磷酸Guanosine-5'-monophosphate | 3.35 | 1.45 | 上调Up | ko00230、ko01100、ko01232、ko01523、ko04022、ko04740、ko04742、ko04744 | |
| 二十二碳六烯酸Docosahexaenoic acid (DHA) | 0.49 | 1.41 | 下调Down | ko01040 | |
NC组 vs ME组 NC group vs ME group | 5'-腺嘌呤核苷酸5'-adenosine monophosphate | 8.19 | 1.58 | 上调Up | ko00230、ko01100、ko01232、ko01240、ko01523、ko04022、ko04024、ko04068、ko04150、ko04151、ko04152、ko04211、ko04740、ko04742、ko04923、ko04924、ko04925、ko04927、ko04928、ko04934、ko05012、ko05022、ko05032 |
| 2'-脱氧鸟苷2'-deoxyguanosine | 2.01 | 1.45 | 上调Up | ko00230、ko01100、ko01232、ko02010 | |
2'-脱氧鸟苷5'-一磷酸 2'-deoxyguanosine 5'-monophosphate (dGMP) | 3.70 | 1.17 | 上调Up | ko00230、ko01100、ko01232、ko02010 | |
| 鸟苷5'-单磷酸Guanosine-5'-monophosphate | 4.64 | 1.35 | 上调Up | ko00230、ko01100、ko01232、ko01523、ko04022、ko04740、ko04742、ko04744 | |
| 肌苷5'-单磷酸Inosine 5'-monophosphate | 3.62 | 1.39 | 上调Up | ko00230、ko01100、ko01232、ko01240、ko01523、ko04742 | |
| 二十二碳六烯酸Docosahexaenoic acid (DHA) | 0.48 | 1.38 | 下调Down | ko01040 | |
| NC组 vs HE组NC group vs HE group | 2,5-二羟基苯甲酸2,5-dihydroxybenzoic acid | 0.48 | 2.12 | 下调Down | ko00350、ko01100 |
| 3-羟基苯乙酸3-hydroxyphenylacetic acid | 2.39 | 1.83 | 上调Up | ko00350、ko00360、ko01100 | |
| 二十二碳六烯酸Docosahexaenoic acid (DHA) | 0.38 | 1.74 | 下调Down | ko01040 | |
ME组 vs LE组 ME group vs LE group | 鹅去氧胆酸Chenodeoxycholic acid | 0.20 | 1.56 | 下调Down | ko00120、ko01100、ko04976 |
| 脱氧胆酸Deoxycholic acid | 0.20 | 1.64 | 下调Down | ko04976 | |
| 2'-脱氧鸟苷2'-deoxyguanosine | 0.48 | 1.60 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 次黄嘌呤Hypoxanthine | 0.50 | 1.70 | 下调Down | ko00230、ko01100、ko01232 | |
| 肌苷Inosine | 0.46 | 1.89 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 牛磺鹅去氧胆酸Taurochenodesoxycholic acid | 0.33 | 2.01 | 下调Down | ko00120、ko04976、ko04979 | |
HE组 vs LE组 HE group vs LE group | 2'-脱氧肌苷2'-deoxyinosine | 0.43 | 2.03 | 下调Down | ko00230、ko01100、ko01232、ko02010 |
| 2'-脱氧鸟苷2'-deoxyguanosine | 0.45 | 1.47 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 甘氨鹅脱氧胆酸Glycochenodeoxycholic acid | 3.44 | 1.58 | 上调Up | ko00120、ko04976、ko04979 | |
| 鸟苷Guanosine | 0.34 | 1.17 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 次黄嘌呤Hypoxanthine | 0.48 | 1.27 | 下调Down | ko00230、ko01100、ko01232 | |
| 肌苷Inosine | 0.39 | 1.29 | 下调Down | ko00230、ko01100、ko01232、ko02010 | |
| 牛磺鹅去氧胆酸Taurochenodesoxycholic acid | 0.20 | 1.38 | 下调Down | ko00120、ko04976、ko04979 | |
HE组 vs ME组 HE group vs ME group | 11,12-双羟基-5Z,8Z,14Z-二十二碳三烯酸 11,12-dihydroxy-5Z,8Z,14Z-docosatrienoic acid | 3.18 | 1.07 | 上调Up | ko00590、ko01100、ko04726 |
8,9-双羟基-5Z,11Z,14Z-二十二碳三烯酸 8,9-dihydroxy-5Z,11Z,14Z-docosatrienoic acid | 3.18 | 1.07 | 上调Up | ko00590、ko01100、ko04726 | |
15-脱氧-δ-12,14-前列腺素J2 15-deoxy-δ-12,14-prostaglandin J2 | 4.58 | 1.27 | 上调Up | ko00590 |
图4 血清生化及抗氧化与血浆代谢组(差异代谢物)的相关性“*”表示显著相关(P<0.05)。“*” means significant correlation (P<0.05).
Fig.4 Correlations between serum biochemistry, antioxidants, plasma metabolome (differential metabolites)
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