Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (10): 92-104.DOI: 10.11686/cyxb2020360
Previous Articles Next Articles
Kai YANG(), Juan SHI(), Yu-tao YUAN, Li-ting WANG
Received:
2020-07-28
Revised:
2020-09-27
Online:
2021-09-16
Published:
2021-09-16
Contact:
Juan SHI
Kai YANG, Juan SHI, Yu-tao YUAN, Li-ting WANG. Pathogen identification and cell physiological changes of Trifolium repens leaves infected with powdery mildew[J]. Acta Prataculturae Sinica, 2021, 30(10): 92-104.
温度 Temperature (℃) | 孢子萌发率 Spore germination rate (%) | ||||
---|---|---|---|---|---|
4 h | 6 h | 8 h | 12 h | 24 h | |
5 | 0.53C | 2.17C | 2.97B | 5.45B | 7.55B |
10 | 0.98C | 4.77C | 8.27B | 16.00B | 17.43B |
15 | 7.01B | 19.00B | 27.40A | 33.15A | 40.47A |
20 | 9.42AB | 18.55B | 21.50A | 32.29A | 35.85A |
25 | 11.57A | 28.21A | 31.25A | 37.76A | 47.53A |
28 | 2.54C | 8.80C | 9.63B | 14.83B | 18.65B |
30 | 0.97C | 4.62C | 6.78B | 7.19B | 9.15B |
Table 1 Effects of different temperatures on the conidia germination of powdery mildew in T. repens
温度 Temperature (℃) | 孢子萌发率 Spore germination rate (%) | ||||
---|---|---|---|---|---|
4 h | 6 h | 8 h | 12 h | 24 h | |
5 | 0.53C | 2.17C | 2.97B | 5.45B | 7.55B |
10 | 0.98C | 4.77C | 8.27B | 16.00B | 17.43B |
15 | 7.01B | 19.00B | 27.40A | 33.15A | 40.47A |
20 | 9.42AB | 18.55B | 21.50A | 32.29A | 35.85A |
25 | 11.57A | 28.21A | 31.25A | 37.76A | 47.53A |
28 | 2.54C | 8.80C | 9.63B | 14.83B | 18.65B |
30 | 0.97C | 4.62C | 6.78B | 7.19B | 9.15B |
光照时间 Illumination treatment | 孢子萌发率Spore germination rate | ||||
---|---|---|---|---|---|
4 h | 6 h | 8 h | 12 h | 24 h | |
全光照 Full illumination | 20.06A | 36.88A | 40.75A | 43.40A | 45.82A |
光暗交替 Half illumination | 13.90AB | 27.54A | 30.12A | 36.66A | 39.96A |
全黑暗 Total darkness | 8.33B | 20.98A | 27.03A | 30.17A | 33.87A |
Table 2 Effect of different lights on the conidia germination of powdery mildew in T. repens (%)
光照时间 Illumination treatment | 孢子萌发率Spore germination rate | ||||
---|---|---|---|---|---|
4 h | 6 h | 8 h | 12 h | 24 h | |
全光照 Full illumination | 20.06A | 36.88A | 40.75A | 43.40A | 45.82A |
光暗交替 Half illumination | 13.90AB | 27.54A | 30.12A | 36.66A | 39.96A |
全黑暗 Total darkness | 8.33B | 20.98A | 27.03A | 30.17A | 33.87A |
pH | 孢子萌发率Spore germination rate | ||||
---|---|---|---|---|---|
4 h | 6 h | 8 h | 12 h | 24 h | |
4.0 | 2.75CD | 10.87BC | 12.54C | 15.20B | 15.78B |
5.0 | 2.94CD | 11.12BC | 14.25B | 19.66B | 25.82B |
6.0 | 11.08B | 17.73AB | 23.20B | 32.43A | 37.51A |
7.0 | 16.86A | 24.77A | 35.97A | 40.99A | 45.50A |
8.0 | 8.01BC | 15.02B | 23.11B | 31.56A | 37.12A |
9.0 | 2.13D | 4.95C | 7.50C | 12.75B | 18.72B |
Table 3 Effects of different pH on the conidia germination of powdery mildew in T. repens (%)
pH | 孢子萌发率Spore germination rate | ||||
---|---|---|---|---|---|
4 h | 6 h | 8 h | 12 h | 24 h | |
4.0 | 2.75CD | 10.87BC | 12.54C | 15.20B | 15.78B |
5.0 | 2.94CD | 11.12BC | 14.25B | 19.66B | 25.82B |
6.0 | 11.08B | 17.73AB | 23.20B | 32.43A | 37.51A |
7.0 | 16.86A | 24.77A | 35.97A | 40.99A | 45.50A |
8.0 | 8.01BC | 15.02B | 23.11B | 31.56A | 37.12A |
9.0 | 2.13D | 4.95C | 7.50C | 12.75B | 18.72B |
1 | Wang Z Y, Shu J H, Chen Y, et al.Analysis of Trifolium repens DNA methylation of Guizhou different regions by MSAP. Genomics and Applied Biology, 2020, 39(4): 1741-1750. |
王子苑, 舒健虹, 陈莹, 等. 贵州不同地区白三叶基因组甲基化MSAP分析. 基因组学与应用生物学, 2020, 39(4): 1741-1750. | |
2 | Huang Y M, Zhang K, Sun L X, et al.Effects of Trifolium repens invasion on soil animals in an urban turf ecosystem. Acta Ecologica Sinica, 2018, 38(23): 8489-8499. |
黄玉梅, 张凯, 孙凌霞, 等. 白三叶(Trifolium repens)入侵对城市草坪生态系统土壤动物的影响. 生态学报, 2018, 38(23): 8489-8499. | |
3 | Liang Z, Jiang S J, Wei L, et al. Progress in research on Trifolium genetic engineering. Acta Prataculturae Sinica, 2009, 18(2): 205-211. |
梁哲, 姜三杰, 未丽, 等. 三叶草基因工程研究进展. 草业学报, 2009, 18(2): 205-211. | |
4 | Chen B S. Forage crop cultivation. Beijing: China Agriculture Press, 2001: 224-233. |
陈宝书. 牧草饲料作物栽培学. 北京: 中国农业出版社, 2001: 224-233. | |
5 | Li Y X, Zhang T B, Du H L, et al. The allelopathy of five pine needle extracts on Trifolium repens L. Journal of Nuclear Agricultural Sciences, 2020, 34(7): 1606-1612. |
李雅馨, 张天宝, 杜慧玲, 等. 5种松针浸提液对白三叶草的化感作用. 核农学报, 2020, 34(7): 1606-1612. | |
6 | Jin Z M, Sha W. Study on drought resistance of Trifolium repens Linn seedlings. Northern Horticulture, 2010(18): 50-52. |
金忠民, 沙伟. 白三叶抗旱生理的研究. 北方园艺, 2010(18): 50-52. | |
7 | Li Z, Peng Y, Yin S X, et al. Effects of exogenous mannose application on drought tolerance, sugars, and sugar alcohol accumulation in white clover. Acta Prataculturae Sinica, 2019, 28(12): 85-93. |
李州, 彭燕, 尹淑霞, 等. 甘露糖对白三叶抗旱性、糖及糖醇类代谢物积累的影响. 草业学报, 2019, 28(12): 85-93. | |
8 | Qu L L, Wang J J. Research progress on stress resistance of Trifolium repens. Chinese Journal of Grassland, 2020, 42(2): 155-161. |
屈璐璐, 王俊杰. 白三叶抗逆性研究进展. 中国草地学报, 2020, 42(2): 155-161. | |
9 | Lan H Y. Common diseases and insect pests of herbage and their control methods. Contemporary Animal Husbandry, 2018(26): 17-18. |
兰惠英. 牧草常见病虫害及防治方法. 当代畜牧, 2018(26): 17-18. | |
10 | Liu X L, Song C, Du W H. Study on mechanism of resistance to powdery mildew in Minshan red clover. Grassland and Turf, 2011, 31(3): 73-76. |
刘晓玲, 宋超, 杜文华. 红三叶对白粉病抗性机理研究. 草原与草坪, 2011, 31(3): 73-76. | |
11 | Yang F. Identification and prevention of powdery mildew in garden lawn. Xinjiang Agricultural Science and Technology, 2008(3): 62. |
杨芬. 园林草坪白粉病的识别与防治. 新疆农业科技, 2008(3): 62. | |
12 | Sang W J, Liang J, Li Y S. Investigation on powdery mildew inlidence of Trifolium pratense in Guiyang. Journal of Southwest University (Natural Science Edition), 1990(4): 353-355. |
桑维均, 梁建, 李永顺. 贵阳地区红三叶草白粉病病情消长和病菌生物学特性. 西南农业大学学报(自然科学版), 1990(4): 353-355. | |
13 | Yuan Y T, Shi J, Ma X, et al. Identification and biological characteristics of alfalfa powdery mildew fungi. Microbiology China, 2020, 47(11): 3539-3550. |
袁玉涛, 史娟, 马新, 等. 紫花苜蓿白粉病病原菌鉴定及其生物学特性. 微生物学通报, 2020, 47(11): 3539-3550. | |
14 | Li S, Zhu L L, Li J F, et al. Identification of the pathogen causing tomato powdery mildew in Heilongjiang Province. Plant Protection, 2014, 40(4): 112-114, 129. |
李帅, 朱路路, 李景富, 等. 黑龙江省番茄白粉病病原鉴定. 植物保护, 2014, 40(4): 112-114, 129. | |
15 | Zheng L, Wu X Q.Advances on infection structures of plant pathogenic fungi. Journal of Nanjing Forestry University (Natural Sciences Edition) , 2007(1): 90-94. |
郑玲, 吴小芹. 植物病原真菌侵染结构研究进展. 南京林业大学学报(自然科学版), 2007(1): 90-94. | |
16 | Riopel J L. Haustorial initiation and differentiation. New York: Parasitic Plants, Chapman and HallPress, 1995. |
17 | Ren B, Gao X N, Han Q M, et al. Etiology and infection process of Glomerella cingulata causing Glomerella leaf spot of apple. Acta Phytophylacica Sinica, 2014, 41(5): 608-614. |
任斌, 高小宁, 韩青梅, 等. 苹果炭疽叶枯病病原(Glomerella cingulata)及其侵染过程. 植物保护学报, 2014, 41(5): 608-614. | |
18 | Yang R L, Liu J Y, Lv X, et al.Micro-and ultrastructural changes of wheat leaf cells induced by powdery mildew infection. Acta Botanica Boreali-Occidentalia Sinica, 2001(2): 293-296, 400-401. |
杨若林, 刘建云, 吕欣, 等. 白粉菌侵染对小麦叶片显微及超微结构的影响. 西北植物学报, 2001(2): 293-296, 400-401. | |
19 | Zhang Z L, Qu W J. Experimental instruction in plant physiology (3rd Edition). Beijing: Higher Education Press, 2003: 206. |
张志良, 瞿伟菁. 植物生理学实验指导(第3版). 北京: 高等教育出版社, 2003: 206. | |
20 | Kang Z S. Ultrastructure of plant pathogenic fungi. Beijing: China Science and Technology Press, 1996: 1-74. |
康振生. 植物病原真菌超微结构. 北京: 中国科学技术出版社, 1996: 1-74. | |
21 | Li W X, Xiao R G, Lv M M, et al. Establishment and application of real-time PCR for quantitatively detecting Plasmopara viticola in Vitis vinifera. Scientia Agricultura Sinica, 2019, 52(9): 1529-1540. |
李文学, 肖瑞刚, 吕苗苗, 等. 葡萄霜霉病菌实时荧光定量PCR检测体系的建立和应用. 中国农业科学, 2019, 52(9): 1529-1540. | |
22 | Fang Z D. Research methods of plant diseases. Beijing: China Agriculturae Press, 1998: 140-145. |
方中达. 植病研究方法. 北京: 中国农业出版社, 1998: 140-145. | |
23 | Wei J C. Fungus identification manual. Shanghai: Shanghai Scientific & Technical Publishers, 1979: 1-9. |
魏景超. 真菌鉴定手册. 上海: 上海科学技术出版社, 1979: 1-9. | |
24 | Zheng R Y, Yu Y N. Flora of fungi in China, Volume I, powdery mildew. Beijing: Science Press, 1987: 5-13. |
郑儒永, 余永年. 中国真菌志, 第一卷, 白粉菌目. 北京: 科学出版社, 1987: 5-13. | |
25 | Bushnell W R. Aggregation of host cytoplasm and the formation of papillae and haustoria in powdery mildew of barley. Phytopathology, 1975, 65: 310-318. |
26 | Wan S L, Liang P, Liu W B, et al.Cytological analysis of compatible interactions between rubber tree and Oidium heveae. Plant Protection, 2014, 40(3): 26-36. |
万三连, 梁鹏, 刘文波, 等. 橡胶树与白粉病菌(Oidium heveae)亲和互作组织细胞学研究. 植物保护, 2014, 40(3): 26-36. | |
27 | Zhang Y M, Ma H L, Tang Y Z. Structural changes in leaves in Medicago sativa infected with Erysiphe pisi. Acta Prataculturae Sinica, 2017, 26(2): 88-94. |
张咏梅, 马晖玲, 唐云智. 紫花苜蓿叶片受白粉病菌侵染后结构的变化. 草业学报, 2017, 26(2): 88-94. | |
28 | Lu Q, Yang L, Wang H W, et al.Responses of photosynthetic characteristics and chloroplast ultrastructure to salt stress in seedlings of Cornus hongkongensis subsp. elegans. Journal of Nanjing Forestry University (Natural Sciences Edition), 2020, 44(4): 29-36. |
鲁强, 杨玲, 王昊伟, 等. 秀丽四照花光合特性和叶绿体超微结构的盐胁迫响应. 南京林业大学学报(自然科学版), 2020, 44(4): 29-36. | |
29 | Li X F, Ni Z M, Wu Y Y, et al.Effects of salt stress on photosynthetic characteristics and leaf cell structure of ‘Yinhong’ grape seedlings. Acta Ecologica Sinica, 2015, 35(13): 4436-4444. |
李学孚, 倪智敏, 吴月燕, 等. 盐胁迫对‘鄞红’葡萄光合特性及叶片细胞结构的影响. 生态学报, 2015, 35(13): 4436-4444. | |
30 | Kirchhoff H . Chloroplast ultrastructure in plants. New Phytologist, 2019, 223(2): 565-574. |
31 | Van Wijk K J, Kessler F. Plastoglobuli: Plastid microcompartments with integrated functions in metabolism, plastid developmental transitions, and environmental adaptation. Annual Review of Plant Biology, 2017, 68(1): 253-289. |
32 | Liu M, Fang Y L. Effects of heat stress on physiological indexes and ultrastructure of grapevines. Scientia Agricultura Sinica, 2020, 53(7): 1444-1458. |
刘敏, 房玉林. 高温胁迫对葡萄幼树生理指标和超显微结构的影响. 中国农业科学, 2020, 53(7): 1444-1458. | |
33 | Xu W, Wang Y, Yuan Q H, et al. Effect of NaCl on ultrastructure in leaves of white clovers (Trifolium repens L.). Chinese Journal of Grassland, 2013, 35(6): 104-108. |
徐威, 王瑜, 袁庆华, 等. NaCl胁迫对白三叶叶片超微结构的影响. 中国草地学报, 2013, 35(6): 104-108. | |
34 | Shi J, Wang H R, Zhong S L.Ultrastructural characteristics of compatible Pseudopeziza medicaginis interaction with alfalfa leaf. Acta Prataculturae Sinica, 2012, 21(5): 122-127. |
史娟, 王华荣, 钟少林. 苜蓿假盘菌与苜蓿叶片亲和性互作的超微结构特征. 草业学报, 2012, 21(5): 122-127. | |
35 | Chen S H, Lu Z Q. Occurrence and control measures of powdery mildew of red clover in artificial pasture. Journal of Grassland and Forage Science, 1997(2): 47-48. |
陈曙晖, 陆忠权. 人工草场红三叶草白粉病的发生与防治措施. 四川草原, 1997(2): 47-48. | |
36 | Yan Y, Li W P, Gao W J, et al. Application of rDNA ITS sequence analysis in fungus identification. Chinese Journal of Health Laboratory Technology, 2008(10): 1958-1961. |
燕勇, 李卫平, 高雯洁, 等. rDNA-ITS序列分析在真菌鉴定中的应用. 中国卫生检验杂志, 2008(10): 1958-1961. | |
37 | Wang L J, Guo N, Wang W, et al. The soil water content monitoring based on the temperature difference in the Northwest China. Research of Soil and Water Conservation, 2018, 25(5): 330-336. |
王丽娟, 郭铌, 王玮, 等. 基于温度差监测西北地区的土壤相对湿度. 水土保持研究, 2018, 25(5): 330-336. |
[1] | WANG Qiong, DUAN Ting-yu, NAN Zhi-biao. Isolation and identification of an anthracnose pathogen on Vicia sativa [J]. Acta Prataculturae Sinica, 2020, 29(6): 127-136. |
[2] | WANG Chun-ming, YUAN Wei-wei, ZHANG Xiao-jie, ZHOU Tian-wang, GUO Cheng, JIN She-lin. Isolation, identification and biological characteristics of Alternaria brassicicola leaf spot on Orychophragums violaceus [J]. Acta Prataculturae Sinica, 2020, 29(5): 88-97. |
[3] | CHEN Bin, LI Hong-yao, LIU Xiao-wei, XIA Bin, SUN Shao-wen, SUN Ying, HE Miao. Effects of different light intensities on morphogenesis and ultrastructure of Gibasis pellucida leaf [J]. Acta Prataculturae Sinica, 2019, 28(7): 175-185. |
[4] | SUN Hai-rong, CHE Zhao-bi, CHEN Yi-shi, LU Wei-hua, WANG Shu-lin, LI Na-na, XIN Huai-lu. Ecological adaptability of biological traits and population distribution patterns for the ephemeral plant Leontice incerta in desert habitats [J]. Acta Prataculturae Sinica, 2019, 28(7): 198-207. |
[5] | LI Jian-hong, LI Xue-ping, LI Chang-ning, HAN Bing, XU Wan-li, YAO Tuo. Characterization of a plant-growth-promoting rhizosphere bacterium, Gnyt1, and determination of its taxonomic status [J]. Acta Prataculturae Sinica, 2019, 28(5): 55-67. |
[6] | DING Ai-qiang, XÜ Xian-ying, ZHANG Wen, LIU Jiang, FU Li, FU Gui-quan. Soil physicochemical and biological characteristics of Tamarix ramosissima Nebkhas in different degradation degree [J]. Acta Prataculturae Sinica, 2019, 28(2): 1-11. |
[7] | FENG Peng, SUN Li, SHEN Xiao-hui, LI Ru-lai, LI Zeng-jie, LI Zhi-min, ZHENG Hai-yan, JIANG Cheng, YNAG He, LIU Jun-gang, GUO Wei, ZHANG Ying-jun. Effect of different mutagenesis methods on microstructure and ultrastructure of alfalfa [J]. Acta Prataculturae Sinica, 2018, 27(6): 72-80. |
[8] | PU Xiao-jian, TIAN Jiu-sheng, TIAN Xin-hui, DU Wen-hua. Construction of the AFLP linkage map and QTL analysis of powdery mildew resistance in red clover [J]. Acta Prataculturae Sinica, 2018, 27(4): 79-88. |
[9] | YANG Cheng-De, BIAN Jing, CHEN Tai-Xiang, CHEN Xiu-Rong, WANG Han-Qi, YANG Xiao-Li, WANG Yang. Biological characteristics of the Angelica sinensis anthracnose causal agent, Colletotrichum dematium [J]. Acta Prataculturae Sinica, 2017, 26(6): 139-144. |
[10] | HE Chun-Gui, HE Zhen-Fu, WANG Fei. Efficient double cropping pattern of photoperiod-sensitive sorghum-sudangrass hybrids in summer after winter wheat [J]. Acta Prataculturae Sinica, 2017, 26(5): 70-80. |
[11] | LI Jian, LI Mei, GAO Xing-Xiang, FANG Feng, DONG Lian-Hong. Biological characteristics of Lubao No.1 biological control agent (Colletotrichum gloeosporioides) and construction of a T-DNA insertional mutant library [J]. Acta Prataculturae Sinica, 2017, 26(1): 142-148. |
[12] | LI Jian, LI Mei, GAO Xing-Xiang, FANG Feng, DONG Lian-Hong. Isolation and biological characteristics of the biological control fungi BC-1 for Echinochloa crusgalli [J]. Acta Prataculturae Sinica, 2016, 25(8): 164-171. |
[13] | ZHAO Fu-Qiang, ZHANG Hai-Qin, SUN Zong-Hua, JIAO Zhen-Fei, LIU Xiao-Yan, CHEN Wei-Huan, CHEN Guo-Yue, ZHOU Yong-Hong. Resistance of Roegneria kamoji (Poaceae: Triticeae) populations to stripe rust and powdery mildew [J]. Acta Prataculturae Sinica, 2016, 25(4): 149-158. |
[14] | JIA Hui, CHEN Xiu-Rong, LU Guang-Xin, KONG Ya-Li, YANG Cheng-De. Isolation of cellulose-degrading bacteria and determination of their degradation activity [J]. Acta Prataculturae Sinica, 2016, 25(3): 60-66. |
[15] | FANG Qiang-En, ZHANG Bo, LI Yu-Bo, SHI Shang-Li. Adaptive changes in the young leaf cell ultrastructure of crown buds in Medicago sativa (Leguminosae) during overwintering [J]. Acta Prataculturae Sinica, 2016, 25(3): 96-107. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||