Effects of nitrogen forms on growth and nitrogen assimilation and utilization of Buchloe dactyloides

doi:10.11686/cyxb2022025

Abstract

Abstract:

This research explored the nitrogen （N） uptake preference of Buchloe dactyloides， in order to improve the management of this species over the whole growth cycle. The experiment included two cultivars of B. dactyloides （‘Sundancer’ and ‘Texoka’） and four different N regimes （N₀： No N as the control， N₁： Urea， N₂： Nitrate N， N₃： Ammonium N and N₄： a 1∶1 mixture of nitrate N and ammonium N）. The rate of N applied was 500 mg N·kg soil^–1 in each case. It was found that total N content， root nitrate reductase （NR） activity， glutamine synthetase （GS） activity and shoot glutamate synthase （GOGAT） activity of B. dactyloides were significantly improved by adding mixed N （P<0.05）. Shoot nitrate N content of B. dactyloides was significantly improved by adding nitrate N （P<0.05）. Root nitrate N and ammonium N contents of B. dactyloides were significantly improved by adding urea （P<0.05）. The shoot ammonium N content and NR activity of B. dactyloides were significantly improved by adding ammonium N （P<0.05）. Shoot GS activity of B. dactyloides was significantly reduced by adding ammonium N （P<0.05）. Root GOGAT activity of B. dactyloides was significantly reduced by adding nitrate N （P<0.05）. Mixed N can better improve the physiological activity and promote the growth of B. dactyloides， but single nitrogen source will inhibit some nitrogen assimilase activity. Based on these results， it is recommended that 1∶1 mixed nitrogen should be used as the nitrogen source in the production of B. dactyloides. Where the ammonium∶nitrate is not 1∶1， urea should be used as the nitrogen source.

Key words: Buchloe dactyloides, nitrogen form, growth characteristics, nitrogen assimilation, nitrogen use efficiency

Bo-kun ZOU, Xin-ming WANG, Zhang-shan CHU, Xin-hui HUANG, Yu-feng CHEN, Yong-qiang QIAN. Effects of nitrogen forms on growth and nitrogen assimilation and utilization of Buchloe dactyloides[J]. Acta Prataculturae Sinica, 2022, 31(11): 118-127.

Figures/Tables 12

References 28

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处理 Treatments	株高Height
处理 Treatments	7 d	14 d	21 d	28 d	35 d	42 d	49 d	56 d
S-N₀	72.4±2.76cd	85.4±3.16f	90.1±1.76f	100.8±1.21h	103.2±1.95i	105.6±1.46h	108.3±1.77g	110.9±1.29h
S-N₁	83.5±1.29ab	96.9±1.57de	144.9±1.80b	155.4±1.31e	168.9±1.86f	179.7±2.27e	190.1±1.76d	191.7±1.36f
S-N₂	83.7±1.55ab	100.7±3.90cde	153.8±1.34a	169.3±2.83b	182.8±1.23b	198.2±1.47b	205.0±1.58b	209.5±0.98cd
S-N₃	91.9±2.14a	106.1±1.63abc	125.6±3.91d	147.4±0.78f	160.6±2.14g	170.9±2.00f	178.5±0.90e	189.9±1.60f
S-N₄	89.7±2.27a	108.5±2.62ab	144.3±2.91b	163.6±2.38c	176.3±1.19cd	185.5±1.85c	189.4±3.72d	197.8±1.45e
T-N₀	55.0±1.55d	83.1±3.89f	111.1±1.36e	116.0±1.86g	119.4±1.70h	123.0±2.03g	126.1±3.52f	125.7±1.91g
T-N₁	75.5±1.63bcd	96.5±3.76e	142.8±1.90bc	160.1±2.00d	173.1±3.82de	181.3±0.87de	196.4±2.69c	208.2±1.37d
T-N₂	76.4±1.50bc	97.6±2.03de	140.0±1.71c	160.3±1.61d	169.3±2.28ef	184.6±3.27cd	196.4±1.38c	211.6±0.86c
T-N₃	78.7±0.62bc	102.8±1.13bcd	153.4±3.30a	167.8±1.82b	180.1±1.99bc	196.3±2.14b	209.4±0.75a	215.9±3.30b
T-N₄	83.0±1.17abc	110.2±1.49a	156.6±3.69a	173.2±1.92a	189.2±2.21a	205.5±0.71a	211.5±0.80a	218.8±1.01a

处理 Treatments	株高Height
处理 Treatments	7 d	14 d	21 d	28 d	35 d	42 d	49 d	56 d
S-N₀	72.4±2.76cd	85.4±3.16f	90.1±1.76f	100.8±1.21h	103.2±1.95i	105.6±1.46h	108.3±1.77g	110.9±1.29h
S-N₁	83.5±1.29ab	96.9±1.57de	144.9±1.80b	155.4±1.31e	168.9±1.86f	179.7±2.27e	190.1±1.76d	191.7±1.36f
S-N₂	83.7±1.55ab	100.7±3.90cde	153.8±1.34a	169.3±2.83b	182.8±1.23b	198.2±1.47b	205.0±1.58b	209.5±0.98cd
S-N₃	91.9±2.14a	106.1±1.63abc	125.6±3.91d	147.4±0.78f	160.6±2.14g	170.9±2.00f	178.5±0.90e	189.9±1.60f
S-N₄	89.7±2.27a	108.5±2.62ab	144.3±2.91b	163.6±2.38c	176.3±1.19cd	185.5±1.85c	189.4±3.72d	197.8±1.45e
T-N₀	55.0±1.55d	83.1±3.89f	111.1±1.36e	116.0±1.86g	119.4±1.70h	123.0±2.03g	126.1±3.52f	125.7±1.91g
T-N₁	75.5±1.63bcd	96.5±3.76e	142.8±1.90bc	160.1±2.00d	173.1±3.82de	181.3±0.87de	196.4±2.69c	208.2±1.37d
T-N₂	76.4±1.50bc	97.6±2.03de	140.0±1.71c	160.3±1.61d	169.3±2.28ef	184.6±3.27cd	196.4±1.38c	211.6±0.86c
T-N₃	78.7±0.62bc	102.8±1.13bcd	153.4±3.30a	167.8±1.82b	180.1±1.99bc	196.3±2.14b	209.4±0.75a	215.9±3.30b
T-N₄	83.0±1.17abc	110.2±1.49a	156.6±3.69a	173.2±1.92a	189.2±2.21a	205.5±0.71a	211.5±0.80a	218.8±1.01a

处理 Treatments	地上部Shoot								地下部Root							隶属度值Subordinate values	排名Rank
处理 Treatments	PH	BI	NI	AM	NC	NR	GS	GOGAT	BI	NI	AM	NC	NR	GS	GOGAT	隶属度值Subordinate values	排名Rank
S-N₀	0.000	0.000	0.000	0.078	0.077	0.106	0.352	0.285	0.227	0.012	0.000	0.018	0.000	0.462	0.000	0.102	10
S-N₁	0.747	0.562	0.672	0.470	0.810	0.561	0.476	0.531	0.018	1.000	0.862	0.546	0.916	0.241	0.782	0.642	5
S-N₂	0.901	0.753	1.000	0.478	0.857	0.717	0.559	0.577	0.675	0.682	0.318	0.785	0.889	0.072	0.544	0.647	4
S-N₃	0.736	0.541	0.422	1.000	0.730	1.000	0.000	0.462	0.022	0.376	0.356	0.778	0.712	0.064	0.677	0.519	8
S-N₄	0.794	0.523	0.129	0.712	1.000	0.672	1.000	0.831	0.000	0.912	0.498	1.000	0.984	0.353	1.000	0.699	2
T-N₀	0.155	0.102	0.112	0.000	0.000	0.000	0.086	0.000	0.900	0.000	0.133	0.000	0.239	1.000	0.028	0.163	9
T-N₁	0.898	0.752	0.681	0.402	0.764	0.695	0.202	0.646	0.368	0.847	1.000	0.554	1.000	0.297	0.677	0.656	3
T-N₂	0.927	0.732	1.000	0.680	0.887	0.627	0.308	0.723	0.699	0.635	0.482	0.743	0.590	0.072	0.501	0.622	6
T-N₃	0.969	0.809	0.397	0.839	0.812	0.817	0.006	0.585	0.672	0.365	0.402	0.680	0.446	0.000	0.688	0.523	7
T-N₄	1.000	1.000	0.138	0.869	0.933	0.696	0.311	1.000	1.000	0.747	0.727	0.935	0.827	0.896	0.737	0.736	1
权重Weight	0.034	0.066	0.032	0.100	0.099	0.065	0.066	0.049	0.052	0.075	0.072	0.077	0.075	0.050	0.088

处理 Treatments	地上部Shoot								地下部Root							隶属度值Subordinate values	排名Rank
处理 Treatments	PH	BI	NI	AM	NC	NR	GS	GOGAT	BI	NI	AM	NC	NR	GS	GOGAT	隶属度值Subordinate values	排名Rank
S-N₀	0.000	0.000	0.000	0.078	0.077	0.106	0.352	0.285	0.227	0.012	0.000	0.018	0.000	0.462	0.000	0.102	10
S-N₁	0.747	0.562	0.672	0.470	0.810	0.561	0.476	0.531	0.018	1.000	0.862	0.546	0.916	0.241	0.782	0.642	5
S-N₂	0.901	0.753	1.000	0.478	0.857	0.717	0.559	0.577	0.675	0.682	0.318	0.785	0.889	0.072	0.544	0.647	4
S-N₃	0.736	0.541	0.422	1.000	0.730	1.000	0.000	0.462	0.022	0.376	0.356	0.778	0.712	0.064	0.677	0.519	8
S-N₄	0.794	0.523	0.129	0.712	1.000	0.672	1.000	0.831	0.000	0.912	0.498	1.000	0.984	0.353	1.000	0.699	2
T-N₀	0.155	0.102	0.112	0.000	0.000	0.000	0.086	0.000	0.900	0.000	0.133	0.000	0.239	1.000	0.028	0.163	9
T-N₁	0.898	0.752	0.681	0.402	0.764	0.695	0.202	0.646	0.368	0.847	1.000	0.554	1.000	0.297	0.677	0.656	3
T-N₂	0.927	0.732	1.000	0.680	0.887	0.627	0.308	0.723	0.699	0.635	0.482	0.743	0.590	0.072	0.501	0.622	6
T-N₃	0.969	0.809	0.397	0.839	0.812	0.817	0.006	0.585	0.672	0.365	0.402	0.680	0.446	0.000	0.688	0.523	7
T-N₄	1.000	1.000	0.138	0.869	0.933	0.696	0.311	1.000	1.000	0.747	0.727	0.935	0.827	0.896	0.737	0.736	1
权重Weight	0.034	0.066	0.032	0.100	0.099	0.065	0.066	0.049	0.052	0.075	0.072	0.077	0.075	0.050	0.088

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