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Acta Prataculturae Sinica ›› 2023, Vol. 32 ›› Issue (11): 168-178.DOI: 10.11686/cyxb2023012

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Salt tolerance identification and evaluation of a population of wild soybean SP1 mutants at the seedling stage

Zong-chang XU1,5(), Xue-li LU1,5, Yun-chong WEI2, Chen MENG1,5, Meng-chao ZHANG1,3, Yuan-yang ZHANG1,5, Meng WANG2, Ju-ying WANG4, Cheng-sheng ZHANG1,5, Yi-qiang LI1,5()   

  1. 1.Marine Agriculture Research Center,Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266100,China
    2.College of Agriculture,Qingdao Agricultural University,Qingdao 266109,China
    3.College of Agriculture,Shanxi Agricultural University,Jinzhong 034100,China
    4.National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land,Dongying 257345,China
    5.Qingdao Key Laboratory of Resources Mining and Biological Breeding in Coastal Saline-alkali Land,Qingdao 266100,China
  • Received:2023-01-05 Revised:2023-02-27 Online:2023-11-20 Published:2023-09-27
  • Contact: Yi-qiang LI

Abstract:

Soil salinity is one of the major issues affecting agricultural production in many countries, including China. To identify and obtain highly salt-tolerant wild soybean (Glycine soja) genotypes for cultivation in saline-alkali regions, the salt tolerance of spaceflight-mutated wild soybean materials was evaluated. In total, 129 SP1 samples of wild soybean that had undergone spaceflight mutagenesis were treated with 150 mmol·L-1 NaCl and then 11 indexes were measured. The indexes included plant height, hypocotyl length, hypocotyl diameter (stem diameter), chlorophyll content, single leaf dry and fresh weight, and leaf area. The salt tolerance of wild soybean mutant materials was assessed at the seedling stage using multivariate statistical analyses including principal component analysis and cluster analysis. There were significant variations in agronomic traits among the wild soybean mutant materials. The coefficient of variation of the various traits ranged from 6.45% to 51.78%. The most significant correlations among the 11 traits were found between plant height at 7 days and 21 days and leaf area. Based on the first five principal component scores, the D value representing a comprehensive evaluation index was determined. Ten highly salt-tolerant mutant materials, 53 salt-tolerant materials, 63 intermediate salt-tolerant materials, and three salt-sensitive materials were screened based on the D value. Peroxidase activity was identified as a crucial factor in the salt tolerance of wild soybean mutant materials. These salt-resistant materials are promising foundational resources for breeding new, salt-tolerant soybean varieties.

Key words: wild soybean, space mutagenesis, seedling stage, principal component analysis, membership function, comprehensive evaluation