Identification of the BZR gene family in alfalfa and analysis of its transcriptional responses to abiotic stress

doi:10.11686/cyxb2023490

Abstract

Abstract:

Brassinosteroids （BRs） are a class of steroid hormones that influence various developmental and physiological processes in plants. Members of the BRASSINAZOLE-RESISTANT （BZR） transcription factor family are positive regulators of brassinosteroid biosynthesis. The BZR gene family plays a crucial role in regulating plant growth and development， including various aspects of stress resistance. However， the BZR gene family in alfalfa （Medicago sativa）， which is known as the ‘king of forages’， has not been thoroughly studied. In this study， we identified 14 BZR genes in alfalfa， and used bioinformatics tools to analyze the physicochemical properties and secondary and tertiary structures of their encoded proteins. The chromosomal locations， phylogenetic relationships， structures， conserved domains， promoter cis-acting elements， gene family interactions， and transcript levels of the 14 genes in six different tissues of alfalfa， as well as their transcriptional responses to abiotic stresses， were also determined. We found that all 14 BZR genes were expressed in various tissues of alfalfa， with nine genes responding to cold stress， seven responding to salt stress， and seven genes responding drought stress. Notably， three BZR genes （MsBZR05， MsBZR06，and MsBZR12） responded to all three types of stress. The results of this study highlight candidate genes for further research on the BZR gene family.

Key words: BZR gene family, Medicago sativa, bioinformatics analysis, abiotic stress, expression analysis

Ze-bin LI, Yong-zheng QIU, Yan-jie LIU, Jin-qiu YU, Bai-ji WANG, Qian-ning LIU, Yue WANG, Guo-wen CUI. Identification of the BZR gene family in alfalfa and analysis of its transcriptional responses to abiotic stress[J]. Acta Prataculturae Sinica, 2024, 33(11): 106-122.

Figures/Tables 15

References 45

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基因Gene	引物序列 Primer sequence （5'-3'）
MsBZR05	F： ACATTACTCCTTCCTGCCTTCT R： TTGATCCCAGCAAACCATTC
MsBZR06	F： GAACGCATGGATGTGATAGG R： AGCCAGGACTTGGGTTGTAG
MsBZR12	F： CCATTCATCCGCAACATAAC R： GAAAGAGGTGGCGTAACAGG
GAPDH	F： GGCTGCCATCAAGGAGGAAT R： TCCAAGCTCAGCCTCATCAAG

基因Gene	引物序列 Primer sequence （5'-3'）
MsBZR05	F： ACATTACTCCTTCCTGCCTTCT R： TTGATCCCAGCAAACCATTC
MsBZR06	F： GAACGCATGGATGTGATAGG R： AGCCAGGACTTGGGTTGTAG
MsBZR12	F： CCATTCATCCGCAACATAAC R： GAAAGAGGTGGCGTAACAGG
GAPDH	F： GGCTGCCATCAAGGAGGAAT R： TCCAAGCTCAGCCTCATCAAG

基因名称 Gene name	基因ID Gene ID	氨基酸数目 Number of amino acids （aa）	分子量 Molecular weight （D）	等电点 Isoelectric point	疏水性 Hydrophobicity	染色体 Chromosome	亚细胞定位 Subcellular localization
MsBZR01	MS.gene24310.t1	249	27557.01	8.22	-0.581	Chr1.1	细胞核Cell nucleus
MsBZR02	MS.gene26935.t1	249	27540.01	8.82	-0.595	Chr1.3	细胞核Cell nucleus
MsBZR03	MS.gene062710.t1	249	27482.92	8.62	-0.595	Chr1.4	细胞核Cell nucleus
MsBZR04	MS.gene070724.t1	323	34856.61	8.44	-0.705	Chr2.1	细胞核Cell nucleus
MsBZR05	MS.gene070726.t1	316	34141.90	8.65	-0.628	Chr2.1	细胞核Cell nucleus
MsBZR06	MS.gene026895.t1	322	34700.43	8.14	-0.693	Chr2.3	细胞核Cell nucleus
MsBZR07	MS.gene059905.t1	280	29760.82	5.32	-0.519	Chr2.3	细胞核Cell nucleus
MsBZR08	MS.gene059906.t1	328	35427.26	8.64	-0.718	Chr2.3	细胞核Cell nucleus
MsBZR09	MS.gene63375.t1	316	34127.87	8.65	-0.628	Chr2.4	细胞核Cell nucleus
MsBZR10	MS.gene63376.t1	323	34856.61	8.44	-0.705	Chr2.4	细胞核Cell nucleus
MsBZR11	MS.gene047749.t1	323	35322.65	9.42	-0.697	Chr5.1	细胞核Cell nucleus
MsBZR12	MS.gene010465.t1	323	35322.65	9.42	-0.697	Chr5.2	细胞核Cell nucleus
MsBZR13	MS.gene89854.t1	323	35322.65	9.42	-0.697	Chr5.3	细胞核Cell nucleus
MsBZR14	MS.gene59331.t1	323	35322.67	9.42	-0.704	Chr5.4	细胞核Cell nucleus

基因名称 Gene name	基因ID Gene ID	氨基酸数目 Number of amino acids （aa）	分子量 Molecular weight （D）	等电点 Isoelectric point	疏水性 Hydrophobicity	染色体 Chromosome	亚细胞定位 Subcellular localization
MsBZR01	MS.gene24310.t1	249	27557.01	8.22	-0.581	Chr1.1	细胞核Cell nucleus
MsBZR02	MS.gene26935.t1	249	27540.01	8.82	-0.595	Chr1.3	细胞核Cell nucleus
MsBZR03	MS.gene062710.t1	249	27482.92	8.62	-0.595	Chr1.4	细胞核Cell nucleus
MsBZR04	MS.gene070724.t1	323	34856.61	8.44	-0.705	Chr2.1	细胞核Cell nucleus
MsBZR05	MS.gene070726.t1	316	34141.90	8.65	-0.628	Chr2.1	细胞核Cell nucleus
MsBZR06	MS.gene026895.t1	322	34700.43	8.14	-0.693	Chr2.3	细胞核Cell nucleus
MsBZR07	MS.gene059905.t1	280	29760.82	5.32	-0.519	Chr2.3	细胞核Cell nucleus
MsBZR08	MS.gene059906.t1	328	35427.26	8.64	-0.718	Chr2.3	细胞核Cell nucleus
MsBZR09	MS.gene63375.t1	316	34127.87	8.65	-0.628	Chr2.4	细胞核Cell nucleus
MsBZR10	MS.gene63376.t1	323	34856.61	8.44	-0.705	Chr2.4	细胞核Cell nucleus
MsBZR11	MS.gene047749.t1	323	35322.65	9.42	-0.697	Chr5.1	细胞核Cell nucleus
MsBZR12	MS.gene010465.t1	323	35322.65	9.42	-0.697	Chr5.2	细胞核Cell nucleus
MsBZR13	MS.gene89854.t1	323	35322.65	9.42	-0.697	Chr5.3	细胞核Cell nucleus
MsBZR14	MS.gene59331.t1	323	35322.67	9.42	-0.704	Chr5.4	细胞核Cell nucleus

蛋白名称 Protein name	α-螺旋α-helix		β-转角β-turn		无规则卷曲Random coil		延伸链Extended chain
蛋白名称 Protein name	数量Number	占比Ratio （%）	数量Number	占比Ratio （%）	数量Number	占比Ratio （%）	数量Number	占比Ratio （%）
MsBZR01	51	20.48	12	4.82	156	62.65	30	12.05
MsBZR02	52	20.16	14	5.13	160	62.02	32	12.40
MsBZR03	53	21.29	12	4.82	165	66.27	19	7.63
MsBZR04	50	15.48	13	4.02	238	73.68	22	6.81
MsBZR05	51	16.14	13	4.11	221	69.94	31	9.81
MsBZR06	54	16.77	16	4.97	227	70.50	25	7.76
MsBZR07	39	13.93	10	3.57	201	71.79	30	10.71
MsBZR08	50	15.24	14	4.27	246	75.00	18	5.49
MsBZR09	47	14.87	18	5.70	218	68.99	18	5.70
MsBZR10	50	15.48	13	4.02	238	73.68	22	6.81
MsBZR11	58	17.96	18	5.57	222	68.73	18	5.57
MsBZR12	58	17.96	18	5.57	222	68.73	25	7.74
MsBZR13	58	17.96	18	5.57	222	68.73	25	7.74
MsBZR14	48	14.86	19	5.88	224	69.35	32	9.91