Effects of space mutagenesis on the growth of alfalfa （Medicago sativa） seedlings under PEG-6000 simulated drought stress

doi:10.11686/cyxb2024301

Abstract

Abstract:

The aim of this work was to explore the effects of space mutagenesis on the growth of alfalfa （Medicago sativa） seedlings under simulated drought stress. An experiment was conducted in which 14 mutated and 14 non-mutated alfalfa materials were treated with polyethylene glycol 6000 （PEG-6000） to impose drought stress or with distilled water as the control. Growth-related indexes were measured for all the plant materials， and the membership function method was used for multivariate data evaluation. The results showed that the plant height， base stem diameter， root length， fresh weight per plant， leaf area， leaf circumference， leaf length， and leaf width of most materials was lower under drought stress than in the control， whereas the SPAD value was higher in the drought-stressed plants than in the control plants. Compared with the non-mutated materials， the most mutated materials showed lower levels of inhibition under drought stress， and higher SPAD values. With the exception of plant height， the growth indexes of mutated Juneng 7 were significantly higher than those of its non-mutated counterpart （P<0.05）. In addition， the base stem diameter and leaf length of mutated WL354， the leaf area， leaf circumference， leaf length， and SPAD value of mutated DS310FY， the leaf area， leaf width， and SPAD value of mutated Yanbao， the leaf area， leaf length， leaf width， and SPAD value of mutated WL343， the leaf length and SPAD value of mutated Gannong No.3， the leaf width of mutated Huangguan， and the SPAD value of mutated Zhongmu No.1， Nanmu 501， and Adina were all significantly higher than those of their respective non-mutated counterparts （P<0.05）. The base stem diameter of Adina， fresh weight per plant of Gannong No.6， leaf area， leaf circumference， leaf length of Gannong No.4， and leaf width and SPAD value of WL354 were significantly higher than those of their respective mutated counterparts （P<0.05）. We conducted a membership function analysis based on the growth indexes of the materials at the seedling stage under drought stress， and obtained a comprehensive score for each material. The average scores of eight mutated materials （Gannong No.3， DS310FY， Juneng 7， Nanmu 501， Huangguan， Yanbao， WL343 and Adina） were higher than those of their non-mutated counterparts. These results indicate that the drought resistance of these eight materials was improved by space mutagenesis.

Key words: space mutagenesis, PEG-6000, drought stress, alfalfa, seedling stage, growth

Yan-xia ZENG, Zhi-long CHEN, Ji-hong SHANG, Xiao-di SHA, Juan WU, Cai-jin CHEN. Effects of space mutagenesis on the growth of alfalfa （Medicago sativa） seedlings under PEG-6000 simulated drought stress[J]. Acta Prataculturae Sinica, 2025, 34(6): 59-69.

Figures/Tables 11

References 28

1	Chen H Y， Huang R， Zhao L， et al. Effects of Cp2-EPS on the morphology and photosynthesis characteristics of alfalfa seedlings under salt stress. Acta Agrestia Sinica， 2024， 32（1）： 229-238.
	陈海雁，黄荣，赵亮，等. Cp2-EPS对盐胁迫下紫花苜蓿苗期形态及光合特性的影响. 草地学报， 2024， 32（1）： 229-238.
2	Fu C M. Effect of spatial mutagenesis on drought resistance of Medicago sativa Deqin. Kunming： Yunnan Agricultural University， 2023.
	伏春美. 空间诱变对德钦紫花苜蓿抗旱性的影响. 昆明：云南农业大学， 2023.
3	Yang H S， Wang Y R， Chang G Z， et al. A study on the space mutation of forages. Chinese Journal of Grassland， 2015， 37（1）： 104-110.
	杨红善，王彦荣，常根柱，等. 牧草的航天诱变研究. 中国草地学报， 2015， 37（1）： 104-110.
4	Yang H S. Breeding of a new alfalfa variety with multifoliate traits by space mutation. Lanzhou： Lanzhou University， 2018.
	杨红善. 航天诱变多叶紫花苜蓿新品种选育研究. 兰州：兰州大学， 2018.
5	Ren W B， Wang M， Chen L B， et al. Effects of spaceflight on seed germination and seedling growth for different alfalfa lines. Seed， 2009， 28（1）： 1-2， 6.
	任卫波，王蜜，陈立波，等. 卫星搭载对不同品系紫花苜蓿种子萌发及其幼苗生长的影响. 种子， 2009， 28（1）： 1-2， 6.
6	Zhang L Q， Jia X H， Zhao J W. Effects of PEG simulated drought stress on seed germination and seedling growth of alfalfa. Molecular Plant Breeding， 2020， 18（11）： 3759-3764.
	张立全，贾旭慧，赵静玮. PEG模拟干旱胁迫对紫花苜蓿种子发芽及幼苗生长的影响. 分子植物育种， 2020， 18（11）： 3759-3764.
7	Zeng Z T. Germination ability of 3 alfalfa under drought stress and response of alfalfa growth. Lanzhou： Gansu Agricultural University， 2018.
	曾泽堂. 模拟干旱胁迫环境下3种紫花苜蓿的萌芽能力与紫花苜蓿生长. 兰州：甘肃农业大学， 2018.
8	Hui Z L， Li Z L， Liu W Y， et al. Effects of seed soaking in fulvic acid solution on seed germination and seedling growth in Medicago sativa under PEG simulated drought stress. Acta Botanica Boreali-Occidentalia Sinica， 2013， 33（8）： 1621-1629.
	回振龙，李自龙，刘文瑜，等. 黄腐酸浸种对PEG模拟干旱胁迫下紫花苜蓿种子萌发及幼苗生长的影响. 西北植物学报， 2013， 33（8）： 1621-1629.
9	Cheng B， Hu S R， Gao Y， et al. Drought resistance of 5 alfalfa species at germination period under PEG simulated drought stress. Journal of Northwest A&F University （Natural Science Edition）， 2019， 47（1）： 53-59.
	程波，胡生荣，高永，等. PEG模拟干旱胁迫下5种紫花苜蓿萌发期抗旱性的评估. 西北农林科技大学学报（自然科学版）， 2019， 47（1）： 53-59.
10	Li H X， Ye Z B， Liao X Y， et al. Effect of microgravity in the space on the growth and development of tomato. Journal of Huazhong Agricultural University， 1994（3）： 310-312.
	李汉霞，叶志彪，廖祥云，等. 空间微重力处理对番茄生长发育的影响. 华中农业大学学报， 1994（3）： 310-312.
11	Li S J， Zhu Y P， Sun K M， et al. The present status and prospect of space breeding in China. Chinese Agricultural Science Bulletin， 2005（1）： 159-162.
	李世娟，诸叶平，孙开梦，等. 中国太空育种现状及其前景展望. 中国农学通报， 2005（1）： 159-162.
12	Li J， Ren W B， Guo H Q， et al. Effect of space flight factors on the peroxidase polymorphism and its activity of alfalfa. Seed， 2012， 31（4）： 46-48.
	李晶，任卫波，郭慧琴，等. 空间诱变对紫花苜蓿过氧化物同工酶影响. 种子， 2012， 31（4）： 46-48.
13	Li Q. Identification of drought tolerance and expression analysis of related genes in seedlings of Medicago falcata. Urumqi： Xinjiang Agricultural University， 2018.
	李倩. 黄花苜蓿苗期耐旱性鉴定及相关基因表达分析. 乌鲁木齐：新疆农业大学， 2018.
14	Wang J Y， Xu W N， Su Y， et al. Effects of drought stress on drought resistance of different Medicago falcata L. germplasm at seedling stage. Guizhou Agricultural Sciences， 2023， 51（11）： 14-24.
	王江银，徐婉宁，苏洋，等. 干旱胁迫对不同苜蓿种质苗期抗旱性的影响. 贵州农业科学， 2023， 51（11）： 14-24.
15	Wu J X， Zeng R Q， Yin H L， et al. Mechanisms of plant response to water stress. Modern Agricultural Research， 2024， 30（10）： 64-68.
	吴佳芯，曾冉琦，尹会兰，等. 植物对水分胁迫的响应机制.现代农业研究， 2024， 30（10）： 64-68.
16	Chen F Q， Ha X， Li Y J， et al. Evaluation of drought resistance of 23 alfalfa germplasm materials during seedling stage under PEG simulated drought stress. Grassland and Turf， 1-11［2024-10-23］. http：//kns.cnki.net/kcms/detail/62.1156.s.20240620.1602.012.html.
	陈奋奇，哈雪，李亚君，等. PEG模拟干旱胁迫下23份紫花苜蓿种质材料苗期抗旱性评价. 草原与草坪， 1-11［2024-10-23］. http：//kns.cnki.net/kcms/detail/62.1156.s.20240620.1602.012.html.
17	Han R H， Jiang C， Dong Z X， et al. Comprehensive evaluation of drought resistance of 47 Medicago germplasm materials under drought stress. Chinese Journal of Grassland， 2017， 39（4）： 27-35.
	韩瑞宏，蒋超，董朝霞，等. 47份苜蓿种质材料抗旱性综合评价. 中国草地学报， 2017， 39（4）： 27-35.
18	Zhang C M， Shi S L， Liu Z， et al. Effects of drought stress on the root morphology and anatomical structure of alfalfa （Medicago sativa） varieties with differing drought-tolerance. Acta Prataculturae Sinica， 2019， 28（5）： 79-89.
	张翠梅，师尚礼，刘珍，等. 干旱胁迫对不同抗旱性苜蓿品种根系形态及解剖结构的影响. 草业学报， 2019， 28（5）： 79-89.
19	Nan S R， Luo Y Z， Yu S M， et al. Effects of rewatering after drought stress on photosynthesis and chlorophyll fluorescence of Medicago sativa cv. xingjiangdaye seedlings. Acta Agrestia Sinica， 2022， 30（5）： 1141-1149.
	南思睿，罗永忠，于思敏，等. 干旱胁迫后复水对新疆大叶苜蓿幼苗光合和叶绿素荧光的影响. 草地学报， 2022， 30（5）： 1141-1149.
20	Chen L， Wang J， Qiu X， et al. Difference of physiological responses and transcriptional regulation of alfalfa with different drought tolerances under drought stress. Acta Agronomica Sinica， 2023， 49（8）： 2122-2132.
	陈力，王靖，邱晓，等. 不同耐旱性紫花苜蓿干旱胁迫下生理响应和转录调控的差异研究. 作物学报， 2023， 49（8）： 2122-2132.
21	Zou C J， Han S J， Xu W D， et al. Eco-physiological responses of Picea mangolica ecotypes to drought stress. Chinese Journal of Applied Ecology， 2003， 14（9）： 1446-1450.
	邹春静，韩士杰，徐文铎，等. 沙地云杉生态型对干旱胁迫的生理生态响应. 应用生态学报， 2003， 14（9）： 1446-1450.
22	Hu H G， Liu J X， Guo H L. Spaceflight mutation breeding of China and its application in grass. Acta Prataculturae Sinica， 2006， 15（1）： 15-21.
	胡化广，刘建秀，郭海林. 我国植物空间诱变育种及其在草类植物育种中的应用. 草业学报， 2006， 15（1）： 15-21.
23	Zhang Y W， Han J G， Ren W B， et al. Breeding by spaceflight mutagenesis and its application in forage breeding. Pratacultural Science， 2005， 22（10）： 59-63.
	张蕴微，韩建国，任卫波，等. 植物空间诱变育种及其在牧草上的应用. 草业科学， 2005， 22（10）： 59-63.
24	Xu Y Y， Jia J F， Niu B T. The influences of space condition on three legume forages. Chinese Journal of Space Science， 1996， 16（S1）： 136-141.
	徐云远，贾敬芬，牛炳韬. 空间条件对3种豆科牧草的影响. 空间科学学报， 1996， 16（S1）： 136-141.
25	Yin Q， Wu J H， Chen C. The study on the morphological diversity of Qiancao No.1 Festuca arundinacea with space mutation. Tillage and Cultivation， 2022， 42（4）： 20-23.
	尹琼，吴佳海，陈超. 航天诱变黔草1号高羊茅形态多样性研究. 耕作与栽培， 2022， 42（4）： 20-23.
26	Kuang Q， Zong Y Q， Yang W， et al. The impact of spatial mutagenesis on agronomic traits and nutrient quality of Deqin alfalfa. Acta Agrestia Sinica， 2024， 32（6）： 1962-1967.
	匡倩，宗亚倩，杨文，等. 空间诱变对‘德钦’紫花苜蓿农艺性状和营养品质的影响. 草地学报， 2024， 32（6）： 1962-1967.
27	Hu J， Shi F L， Gao C P. Variation analysis of single plant agronomic traits of space-induced mutant SP₁in Festuca rubra. Chinese Journal of Grassland， 2022， 44（8）： 46-51.
	胡洁，石凤翎，高翠萍. 紫羊茅空间诱变SP₁代单株材料田间农艺性状的变异分析. 中国草地学报， 2022， 44（8）： 46-51.
28	Hou R H， Du K， Huang W Y， et al. Evaluation of drought resistance of 10 wild Medicago ruthenica L. materials from different provenances at germination period. Acta Agrestia Sinica， 2024， 32（2）： 489-494.
	侯瑞虹，杜柯，黄伟业，等. 10份不同种源野生花苜蓿材料种子萌发期抗旱性评价. 草地学报， 2024， 32（2）： 489-494.

材料名称 Material name	各指标隶属函数值The subordinative function values of each index										排序Rank
材料名称 Material name	株高 Plant height	基茎粗 Stem size	根长 Root length	单株鲜重 Fresh weight per plant	叶面积 Leaf area	叶周长 Leaf circumference	叶长 Leaf length	叶宽 Leaf width	SPAD值 SPAD value	平均 Average	排序Rank
劲能（未诱变）Jinneng （unmutagenesis）	0.3796	0.5000	0.6830	0.4362	0.6426	0.6409	0.5480	0.7293	0.6881	0.5831	1
劲能（诱变）Jinneng （mutagenesis）	0.3981	0.5167	0.7054	0.3253	0.6740	0.6339	0.5296	0.7178	0.7423	0.5826	2
中苜1号（未诱变）Zhongmu No.1 （unmutagenesis）	0.5833	0.2900	0.6696	0.3909	0.5050	0.7452	0.6001	0.7310	0.4518	0.5519	1
中苜1号（诱变）Zhongmu No.1 （mutagenesis）	0.3241	0.0000	0.6741	0.2840	0.5713	0.4923	0.4369	0.6486	0.7179	0.4610	2
甘农3号（未诱变）Gannong No.3 （unmutagenesis）	0.3796	0.3033	0.5893	0.1636	0.1070	0.2146	0.2053	0.2618	0.4286	0.2948	2
甘农3号（诱变）Gannong No.3 （mutagenesis）	0.3194	0.3067	0.8036	0.2572	0.3916	0.3909	0.6266	0.2773	0.9982	0.4857	1
WL354（未诱变）WL354 （unmutagenesis）	0.1574	0.1867	0.5357	0.3086	0.1887	0.1665	0.2244	0.3735	0.8774	0.3354	1
WL354（诱变）WL354 （mutagenesis）	0.0417	0.5367	0.4286	0.1379	0.2283	0.2846	0.4820	0.2551	0.5982	0.3326	2
DS310FY（未诱变）DS310FY （unmutagenesis）	0.0046	0.4367	0.3884	0.4568	0.0583	0.1725	0.2536	0.0188	0.4869	0.2530	2
DS310FY（诱变）DS310FY （mutagenesis）	0.0000	0.5567	0.3482	0.4444	0.3102	0.5240	0.4466	0.0132	0.9256	0.3966	1
甘农6号（未诱变）Gannong No.6 （unmutagenesis）	0.1296	0.4633	0.4866	0.2531	0.2046	0.1327	0.2286	0.3298	0.7595	0.3320	1
甘农6号（诱变）Gannong No.6 （mutagenesis）	0.0972	0.4900	0.6161	0.0021	0.2931	0.0000	0.2224	0.2695	0.6077	0.2887	2
甘农4号（未诱变）Gannong No.4 （unmutagenesis）	0.3657	0.3667	0.6384	0.1975	0.3512	0.6418	0.6159	0.5641	0.2750	0.4463	1
甘农4号（诱变）Gannong No.4 （mutagenesis）	0.0787	0.4433	0.4598	0.1523	0.1554	0.2574	0.1833	0.3336	0.2548	0.2576	2
中苜3号（未诱变）Zhongmu No.3 （unmutagenesis）	0.5046	0.5567	0.9688	0.7160	0.4538	0.5482	0.5814	0.6316	0.2899	0.5834	1
中苜3号（诱变）Zhongmu No.3 （mutagenesis）	0.5556	0.4233	0.9554	0.4177	0.4486	0.4732	0.4339	0.4807	0.4720	0.5178	2
巨能7（未诱变）Juneng 7（unmutagenesis）	0.2824	0.2633	0.3705	0.3827	0.4085	0.4152	0.3420	0.4738	0.3863	0.3694	2
巨能7（诱变）Juneng 7 （mutagenesis）	0.4907	0.7000	0.8750	0.6790	0.7677	0.8753	0.7086	0.6610	0.5327	0.6989	1
南苜501（未诱变）Nanmu 501 （unmutagenesis）	0.2593	0.5000	0.6429	0.1965	0.0000	0.1991	0.0196	0.0434	0.2732	0.2371	2
南苜501（诱变）Nanmu 501 （mutagenesis）	0.1343	0.6167	0.5357	0.1029	0.0220	0.2395	0.1432	0.0012	0.5292	0.2583	1
皇冠（未诱变）Huangguan （unmutagenesis）	0.4120	0.5933	0.8259	0.6996	0.4688	0.4542	0.3330	0.5510	0.6256	0.5515	2
皇冠（诱变）Huangguan （mutagenesis）	0.5139	0.7133	0.7723	0.7130	0.6364	0.5256	0.6009	0.7634	0.6643	0.6559	1
盐宝（未诱变）Yanbao （unmutagenesis）	0.5417	0.5400	0.5670	0.3673	0.3667	0.4168	0.4315	0.4002	0.4708	0.4558	2
盐宝（诱变）Yanbao （mutagenesis）	0.5000	0.5700	0.6161	0.6276	0.7605	0.5502	0.6685	0.7549	0.6756	0.6359	1
WL343（未诱变）WL343 （unmutagenesis）	0.3171	0.6100	0.3884	0.4516	0.3550	0.2812	0.2210	0.3473	0.3524	0.3693	2
WL343（诱变）WL343 （mutagenesis）	0.4861	0.5600	0.5580	0.4321	0.5465	0.4333	0.6330	0.5391	0.5762	0.5294	1
阿迪娜（未诱变）Adina （unmutagenesis）	0.0278	0.6833	0.1027	0.3230	0.1813	0.0780	0.0000	0.2440	0.2429	0.2092	2
阿迪娜（诱变）Adina （mutagenesis）	0.2083	0.4133	0.1339	0.3539	0.2830	0.1080	0.2183	0.0500	0.5274	0.2551	1

材料名称 Material name	各指标隶属函数值The subordinative function values of each index										排序Rank
材料名称 Material name	株高 Plant height	基茎粗 Stem size	根长 Root length	单株鲜重 Fresh weight per plant	叶面积 Leaf area	叶周长 Leaf circumference	叶长 Leaf length	叶宽 Leaf width	SPAD值 SPAD value	平均 Average	排序Rank
劲能（未诱变）Jinneng （unmutagenesis）	0.3796	0.5000	0.6830	0.4362	0.6426	0.6409	0.5480	0.7293	0.6881	0.5831	1
劲能（诱变）Jinneng （mutagenesis）	0.3981	0.5167	0.7054	0.3253	0.6740	0.6339	0.5296	0.7178	0.7423	0.5826	2
中苜1号（未诱变）Zhongmu No.1 （unmutagenesis）	0.5833	0.2900	0.6696	0.3909	0.5050	0.7452	0.6001	0.7310	0.4518	0.5519	1
中苜1号（诱变）Zhongmu No.1 （mutagenesis）	0.3241	0.0000	0.6741	0.2840	0.5713	0.4923	0.4369	0.6486	0.7179	0.4610	2
甘农3号（未诱变）Gannong No.3 （unmutagenesis）	0.3796	0.3033	0.5893	0.1636	0.1070	0.2146	0.2053	0.2618	0.4286	0.2948	2
甘农3号（诱变）Gannong No.3 （mutagenesis）	0.3194	0.3067	0.8036	0.2572	0.3916	0.3909	0.6266	0.2773	0.9982	0.4857	1
WL354（未诱变）WL354 （unmutagenesis）	0.1574	0.1867	0.5357	0.3086	0.1887	0.1665	0.2244	0.3735	0.8774	0.3354	1
WL354（诱变）WL354 （mutagenesis）	0.0417	0.5367	0.4286	0.1379	0.2283	0.2846	0.4820	0.2551	0.5982	0.3326	2
DS310FY（未诱变）DS310FY （unmutagenesis）	0.0046	0.4367	0.3884	0.4568	0.0583	0.1725	0.2536	0.0188	0.4869	0.2530	2
DS310FY（诱变）DS310FY （mutagenesis）	0.0000	0.5567	0.3482	0.4444	0.3102	0.5240	0.4466	0.0132	0.9256	0.3966	1
甘农6号（未诱变）Gannong No.6 （unmutagenesis）	0.1296	0.4633	0.4866	0.2531	0.2046	0.1327	0.2286	0.3298	0.7595	0.3320	1
甘农6号（诱变）Gannong No.6 （mutagenesis）	0.0972	0.4900	0.6161	0.0021	0.2931	0.0000	0.2224	0.2695	0.6077	0.2887	2
甘农4号（未诱变）Gannong No.4 （unmutagenesis）	0.3657	0.3667	0.6384	0.1975	0.3512	0.6418	0.6159	0.5641	0.2750	0.4463	1
甘农4号（诱变）Gannong No.4 （mutagenesis）	0.0787	0.4433	0.4598	0.1523	0.1554	0.2574	0.1833	0.3336	0.2548	0.2576	2
中苜3号（未诱变）Zhongmu No.3 （unmutagenesis）	0.5046	0.5567	0.9688	0.7160	0.4538	0.5482	0.5814	0.6316	0.2899	0.5834	1
中苜3号（诱变）Zhongmu No.3 （mutagenesis）	0.5556	0.4233	0.9554	0.4177	0.4486	0.4732	0.4339	0.4807	0.4720	0.5178	2
巨能7（未诱变）Juneng 7（unmutagenesis）	0.2824	0.2633	0.3705	0.3827	0.4085	0.4152	0.3420	0.4738	0.3863	0.3694	2
巨能7（诱变）Juneng 7 （mutagenesis）	0.4907	0.7000	0.8750	0.6790	0.7677	0.8753	0.7086	0.6610	0.5327	0.6989	1
南苜501（未诱变）Nanmu 501 （unmutagenesis）	0.2593	0.5000	0.6429	0.1965	0.0000	0.1991	0.0196	0.0434	0.2732	0.2371	2
南苜501（诱变）Nanmu 501 （mutagenesis）	0.1343	0.6167	0.5357	0.1029	0.0220	0.2395	0.1432	0.0012	0.5292	0.2583	1
皇冠（未诱变）Huangguan （unmutagenesis）	0.4120	0.5933	0.8259	0.6996	0.4688	0.4542	0.3330	0.5510	0.6256	0.5515	2
皇冠（诱变）Huangguan （mutagenesis）	0.5139	0.7133	0.7723	0.7130	0.6364	0.5256	0.6009	0.7634	0.6643	0.6559	1
盐宝（未诱变）Yanbao （unmutagenesis）	0.5417	0.5400	0.5670	0.3673	0.3667	0.4168	0.4315	0.4002	0.4708	0.4558	2
盐宝（诱变）Yanbao （mutagenesis）	0.5000	0.5700	0.6161	0.6276	0.7605	0.5502	0.6685	0.7549	0.6756	0.6359	1
WL343（未诱变）WL343 （unmutagenesis）	0.3171	0.6100	0.3884	0.4516	0.3550	0.2812	0.2210	0.3473	0.3524	0.3693	2
WL343（诱变）WL343 （mutagenesis）	0.4861	0.5600	0.5580	0.4321	0.5465	0.4333	0.6330	0.5391	0.5762	0.5294	1
阿迪娜（未诱变）Adina （unmutagenesis）	0.0278	0.6833	0.1027	0.3230	0.1813	0.0780	0.0000	0.2440	0.2429	0.2092	2
阿迪娜（诱变）Adina （mutagenesis）	0.2083	0.4133	0.1339	0.3539	0.2830	0.1080	0.2183	0.0500	0.5274	0.2551	1

[1]	Xiao-Yue WEN, Ying ZHAO, Bao-qiang WANG, Xian WANG, Xiao-lin ZHU, Yi-zhen WANG, Xiao-hong WEI. Expression analysis of AP2/ERFs genes in alfalfa regulated by exogenous NO under drought stress [J]. Acta Prataculturae Sinica, 2025, 34(6): 154-167.
[2]	Qing-qing ZHANG, Xing-yu MA, Yan LU, Guang-Xing ZHAO, Fan-jiang ZENG, Cai-bian HUANG. A study of salt tolerance differences in Cyperus esculentus at different growth stages in a sandy saline soil [J]. Acta Prataculturae Sinica, 2025, 34(6): 168-180.
[3]	Ying-hao ZHANG, Chu-bo LIU, Kun ZHOU, Jia-cun GUO, Shi-peng LIU, Luan-zi SUN. Effects of jujube tree on the growth of alfalfa and orchardgrass in different positions within an orchard [J]. Acta Prataculturae Sinica, 2025, 34(6): 203-212.
[4]	Min ZHANG, Rui YANG, Yi-zhou HUANG, Zhi-xin LIN, Xian-yue ZHENG, Qing-hua LIU, Yu-yun GAO, Dong-mei LIN, Zhan-xi LIN, Ling JIN. Effects of Pennisetum giganteum on the growth performance and intestinal health of finishing Congjiang Xiang pigs [J]. Acta Prataculturae Sinica, 2025, 34(5): 171-188.
[5]	Shuai QI, Yan-li ZHANG, Yong-jie WAN, Wei-qiang NIU, Ji-xin ZHANG, Xue GAO, Da-gan MAO. Effects of soybean straw co-fermented with a bacterium-enzyme mixture on the growth performance， serum indexes， and rumen microorganisms of Hu sheep [J]. Acta Prataculturae Sinica, 2025, 34(5): 189-201.
[6]	Dong-liang SHANG, Hui ZANG. Research progress on the chemical control of annual bluegrass in established golf turf [J]. Acta Prataculturae Sinica, 2025, 34(5): 223-236.
[7]	Wen-jin LIU, Fu-zhen JIANG, Kai-bin QI, Ming-dan SONG, Zheng-peng LI. Effects of different fertilization and sowing amounts on vegetation restoration and soil quality in alpine mining areas and comprehensive evaluation [J]. Acta Prataculturae Sinica, 2025, 34(5): 27-39.
[8]	Kong-qin WEI, Ying-ying ZHANG, Jin-feng HUI, Chun-hui MA, Qian-bing ZHANG. Effect of phosphate-solubilizing bacteria and phosphorus on non-structural carbohydrate content and the carbon∶nitrogen∶phosphorus stoichiometry of alfalfa roots [J]. Acta Prataculturae Sinica, 2025, 34(5): 40-50.
[9]	Ya-qi FENG, Jia-hui CHEN, Jing-ni ZHANG, Chao SUI, Ji-wei CHEN, Zhi-peng LIU, Qiang ZHOU, Wen-xian LIU. Development of high-protein and high-yield associated InDel molecular markers based on re-sequencing in alfalfa [J]. Acta Prataculturae Sinica, 2025, 34(4): 137-149.
[10]	Zhao MA, Xiao-fan LI, Li-qiong SUN, Zhi HUANG, Lei XU, Ting LU, Xiao-qing TANG, Kang-cai WANG. Effects of three endophytic bacteria in the roots of Salvia miltiorrhiza on host growth and medicinal quality [J]. Acta Prataculturae Sinica, 2025, 34(4): 175-188.
[11]	Xiao-feng WANG, Bu-dong MA, Hai-xia HUANG, Yong-zhong LUO, Jian-wei QI, Zhuo DENG. Effects of drought stress and rehydration on the physiological characteristics of Gymnocarpos przewalskii seedlings [J]. Acta Prataculturae Sinica, 2025, 34(4): 93-103.
[12]	Feng ZHANG, Pei-fang CHONG, Xin-guang BAO, Xue-ying WANG. Isolation and identification of four strains of Reaumuria soongorica root zone nitrogen fixing bacteria and their role in seedling growth promotion [J]. Acta Prataculturae Sinica, 2025, 34(3): 144-153.
[13]	Meng-qi WANG, Fei WANG, Wan-lu ZHAO, Yan-qi LIU, Can CUI, Jun-xin YAN. Effects of different concentrations of silicon and calcium on the growth and physiological characteristics of Mentha spicata seedlings [J]. Acta Prataculturae Sinica, 2025, 34(3): 154-163.
[14]	Cai-jin CHEN, Ming-fang BAO, Wen-hu WANG, Ji-hong SHANG, Yan-xia ZENG, Xiao-di SHA, Xin-zhong ZHU, Xue-min WANG, Wen-hui LIU. Current situation and prospects for drought-resistance breeding in Medicago sativa [J]. Acta Prataculturae Sinica, 2025, 34(3): 204-223.
[15]	Xin-yi LIN, Ni WANG, Tuo CHEN, Yi-lan SONG, Yao-dong LU, Zhao-xia DONG. Effects of three plant growth regulators on shade tolerance of Zoysia japonica [J]. Acta Prataculturae Sinica, 2025, 34(3): 224-232.