Genome-wide identification and expression analysis of the Hsp70 gene family in wheat and its ancestral species

doi:10.11686/cyxb2023334

Abstract

Abstract:

Heat shock protein 70 （Hsp70） exerts a crucial influence on plant development and response to biotic and abiotic stresses. In order to explore the evolutionary relationships， functions and expression patterns of the Hsp70 gene family in wheat （Triticum aestivum）， this study conducted a comprehensive bioinformatics analysis of the Hsp70 gene family in Triticum urartu， Aegilops speltoides， Triticum turgidum， Aegilops tauschii and common wheat， and the expression patterns of some Hsp70 genes under different exogenous hormones and environmental stress were analyzed by RT-qPCR. The results showed that， respectively， from the five species， 30， 41， 60， 28 and 94 Hsp70 genes were identified. Phylogenetic analysis showed that the members of the Hsp70 family in the five species were divided into 5 subfamily groups， and the number of members in each group was not equal. Most of the members are distributed in Group I， and most of the Hsp70 members in the same subfamily have similar gene structures and conserved motifs. Further comprehensive analysis of chromosome localization and repetition events of the Hsp70 gene in the five species revealed that the Hsp70 gene was unevenly distributed on each of the chromosomes， and a total of 12 tandem repeat events and 110 fragment replication events were found from the five species， indicating that fragment replication events promoted the expansion of the Hsp70 gene family in wheat. The analysis of cis-acting elements showed that there were various light response elements， stress response elements， hormone response elements and growth as well as development regulatory elements in the promoter region of Hsp70 gene in the five wheat species. In addition， RT-qPCR results indicated that particular Hsp70 genes in the five wheat species had varying degrees of response under different hormone treatments and stress. The expression of eight Hsp70 genes was up-regulated under high temperature and drought stress. The identification of the Hsp70 gene in wheat and its ancestral species and elucidation of its evolution provides a theoretical basis for further research on the function of the Hsp70 gene in wheat growth and development， as well as its response mechanism under stress.

Key words: wheat, Hsp70 gene family, bioinformatics analysis, expression patterns

Yi WU, Ya-lan FENG, Tian-ning WANG, Ji-hao JU, Hui-shu XIAO, Chao MA, Jun ZHANG. Genome-wide identification and expression analysis of the Hsp70 gene family in wheat and its ancestral species[J]. Acta Prataculturae Sinica, 2024, 33(7): 53-67.

Figures/Tables 10

Table 1 Some primers for RT-qPCR of Hsp70 genes

基因号Gene ID	基因名Gene name	正向引物Forward primer （5’-3’）	反向引物Reverse primer （5’-3’）
TraesCS5A02G479300.1	TaHsp70-54	GCCACAGCTGGTGACACTCAC	TGGAATAGAAATCAACTCCCTCG
TraesCS5B02G087700.1	TaHsp70-57	CATCAGTGGCAACCCGAGAG	GAAGTCAATGCCCTCAAACAGC
TraesCS5D02G492900.1	TaHsp70-68	GGTGGCACTTTTGATGTCTCC	CCAAGATGAGTGTCACCAGCC
TRITD5Av1G040700.2	TtHsp70-36	GGACTCTCTCCTCCACTGCG	TGGACAGCAGCCCCGTAG
TRITD5Bv1G039950.1	TtHsp70-44	GCAAAGATGGACAAGAGCACC	TGGACAGCAGCCCCGTAC
XP_048532408.1	TuHsp70-18	GCCACAGCTGGTGACACTCAC	TGGAATAGAAATCAACTCCCTCG
AespeY2032CH5S01G103600.1	AesHsp70-25	CATCAGTGGCAACCCGAGAG	GAAGTCAATGCCCTCAAACAGC
XP_020200619.1	AetHsp70-20	GGTGGCACTTTTGATGTCTCC	CCAAGATGAGTGTCACCAGCC
TraesCS1B02G283900.1	Actin	GTTCCAATCTATGAGGGATACACGC	GAACCTCCACTGAGAACAACATTACC

Fig.1 Phylogenetic tree of Hsp70 gene family members in wheat and its ancestral species

Fig.2 Identification of conserved sequence of Hsp70 family members

Fig.3 Analysis of the phylogenetic relationship and gene structure of Hsp70 gene in wheat and its ancestral species

Fig.4 Chromosomal distribution of Hsp70 gene in wheat and its ancestral species

Fig.5 Chromosomal positions and synteny relationships of Hsp70 genes in wheat and its ancestral species

Fig.6 Homologous analysis of the Hsp70 genes in wheat and its ancestral species

Fig.7 Promoter cis-regulatory element of the Hsp70 genes in wheat and its ancestral species

Fig.8 Expression pattern analysis of Hsp70 gene family members under four hormone treatments

Fig.9 Expression patterns analysis of Hsp70 gene family members under four kinds of stress

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