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草业学报 ›› 2023, Vol. 32 ›› Issue (3): 118-127.DOI: 10.11686/cyxb2022126

• 研究论文 • 上一篇    

棕榈粕替代部分玉米对藏羊母羊小肠形态发育、消化酶活性及抗氧化功能的影响

周力1,2(), 侯生珍1, 王志有1, 杨葆春1, 韩丽娟1, 桂林生1()   

  1. 1.青海大学农牧学院,青海 西宁 810016
    2.宁夏大学农学院,宁夏 银川 750021
  • 收稿日期:2022-03-18 修回日期:2022-05-23 出版日期:2023-03-20 发布日期:2022-12-30
  • 通讯作者: 桂林生
  • 作者简介:E-mail: guilinsheng1234@163.com
    周力(1994-),男,安徽定远人,在读博士。E-mail: 472737885@qq.com
  • 基金资助:
    青海省科技厅帅才科学家项目(2022NK169);青海省农业农村厅藏羊均衡生产关键技术与示范推广(CK216327);青海林草局加强青海湖-祁连山保护项目(K992138);青海省羊产业平台农业生产发展资金(CK2134607);青海黑藏羊生长发育关键基因挖掘及开发研究(2020GN118)

Changes in small intestinal morphology, digestive enzyme activity and antioxidant enzyme activities of female Tibetan sheep after substituting the maize component of a concentrate diet with palm meal

Li ZHOU1,2(), Sheng-zhen HOU1, Zhi-you WANG1, Bao-chun YANG1, Li-juan HAN1, Lin-sheng GUI1()   

  1. 1.College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China
    2.College of Agriculture,Ningxia University,Yinchuan 750021,China
  • Received:2022-03-18 Revised:2022-05-23 Online:2023-03-20 Published:2022-12-30
  • Contact: Lin-sheng GUI

摘要:

试验旨在探究精料补充料中使用不同比例棕榈粕替代玉米对藏羊母羊肠道组织形态学、消化酶活性、pH、脂多糖以及抗氧化能力的影响。选取初始体重相近且健康的2~3月龄高原型藏羊母羊120只,随机分为4组,每组30只,每组6个重复,每个重复5只母羊,分别饲喂0%、15%、18%和21%水平的棕榈粕替代精料中玉米。试验期97 d。结果显示:1)0%组与15%组间小肠各段的绒毛高度、绒毛宽度、隐窝深度、黏膜厚度以及绒毛高度/隐窝深度差异均不显著(P>0.05);2)0%组空肠的α-淀粉酶、纤维素酶、脂肪酶及糜蛋白酶显著或极显著小于15%组(P<0.05或P<0.01);3)0%组空肠的谷胱甘肽过氧化物酶、过氧化氢酶活性显著低于15%组与18%组(P<0.05),相较于21%组差异不显著(P>0.05);4)18%组与21%组空肠的脂多糖含量显著或极显著高于0%组(P<0.05或P<0.01),与15%组相比差异不显著(P>0.05)。由此可见,棕榈粕可替代部分玉米饲喂藏羊母羊,推荐替代玉米的比例为15%。

关键词: 高原型藏羊, 棕榈粕, 玉米, 脂多糖, 肠道, 消化酶活性, 抗氧化指标, pH

Abstract:

This experiment explored the effects on jejunum histomorphology, digestive enzyme activity, pH, lipopolysaccharide levels and antioxidant enzyme activity of substituting the maize component of a concentrate diet with palm meal in female Tibetan sheep. Well bred female Tibetan sheep aged 2-3 months with similar initial weight and in good health were randomly divided into four treatment groups of 30 animals, each comprising six replicate groups of 5 sheep. In the four treatment groups, 0%, 15%, 18% or 21% palm meal were fed to replace maize in the concentrate for a test period is 97 days. It was found that: 1) There were no significant differences in villus height, villus width, crypt depth, mucosal thickness and villus height∶crypt depth between the 0% and 15% maize groups (P>0.05); 2) The activities of α-amylase, cellulase, lipase and chymotrypsin in the jejunum in the 0% maize group were significantly lower than those in 15% maize group (P<0.05 or P<0.01). 3) The activities of glutathione peroxidase and catalase in the jejunum in the 0% maize group were significantly lower than those in the 15% and 18% maize groups (P<0.05), but did not differ significantly from the 21% maize group (P>0.05); 4) The jejunum concentrations of lipopolysaccharide in the 18% and 21% maize groups were significantly higher than in the 0% maize group (P<0.05 or P<0.01), but there was no significant difference compared with the 15% group (P>0.05). In conclusion, palm meal can partially replace maize to feed Tibetan ewes, and the recommended maize substitution rate is 15%.

Key words: plateau Tibetan sheep, palm kernel meal, corn, lipopolysaccharide, intestinal, digestive enzyme activities, antioxidant enzyme activity, pH