草业学报 ›› 2024, Vol. 33 ›› Issue (10): 194-202.DOI: 10.11686/cyxb2023422
• 研究简报 • 上一篇
郭田心(), 阮诗诗, 郭香, 詹佳琦, 梁秋雨, 陈晓阳, 周玮(), 张庆()
收稿日期:
2023-11-14
修回日期:
2024-01-03
出版日期:
2024-10-20
发布日期:
2024-07-15
通讯作者:
周玮,张庆
作者简介:
wzhou@scau.edu.cn基金资助:
Tian-xin GUO(), Shi-shi RUAN, Xiang GUO, Jia-qi ZHAN, Qiu-yu LIANG, Xiao-yang CHEN, Wei ZHOU(), Qing ZHANG()
Received:
2023-11-14
Revised:
2024-01-03
Online:
2024-10-20
Published:
2024-07-15
Contact:
Wei ZHOU,Qing ZHANG
摘要:
为了探究不同酶制剂和植物乳杆菌(LP)复合添加对中药渣青贮发酵品质动态变化的影响,以中药渣(黄芪、山药、茯苓与柴胡)为原料, LP与酶[纤维素酶(CE)、果胶酶(PE)、木聚糖酶(XY)]分别组合添加处理,并设置空白对照(CK),青贮3、7、15、34 d后分析其青贮发酵品质。结果表明:所有处理组在青贮34 d后,pH值、酸性洗涤纤维、中性洗涤纤维相比对照组均显著降低(P<0.05),可溶性碳水化合物含量相比对照组显著提高(P<0.05)。综上所述,添加菌酶复合添加剂可显著降低中药渣青贮后中性洗涤纤维、酸性洗涤纤维含量。随着青贮时间的延长,LP+CE处理组能够增加可溶性碳水化合物含量,且LP+CE和LP+PE处理组能够减少氨态氮含量,有效减少蛋白降解,使中药渣青贮品质得到有效改善。
郭田心, 阮诗诗, 郭香, 詹佳琦, 梁秋雨, 陈晓阳, 周玮, 张庆. 不同复合菌酶添加对中药渣青贮饲料的营养价值及发酵品质的影响[J]. 草业学报, 2024, 33(10): 194-202.
Tian-xin GUO, Shi-shi RUAN, Xiang GUO, Jia-qi ZHAN, Qiu-yu LIANG, Xiao-yang CHEN, Wei ZHOU, Qing ZHANG. Effect of bacterial enzyme complexes on the quality of silage made from Chinese medicine crop residues[J]. Acta Prataculturae Sinica, 2024, 33(10): 194-202.
项目Items | 含量Content |
---|---|
干物质 Dry matter (DM,%) | 33.92±1.42 |
中性洗涤纤维 Neutral detergent fiber (NDF,%DM) | 59.57±3.05 |
酸性洗涤纤维 Acid detergent fiber (ADF,%DM) | 42.35±2.08 |
可溶性碳水化合物 Water-soluble carbohydrates (WSC,%DM) | 7.05±1.64 |
粗蛋白质 Crude protein (CP,%DM) | 7.43±0.62 |
乳酸菌 Lactic acid bacteria (LAB,log10 cfu·g-1 FM) | <2.00 |
酵母菌 Yeast (log10 cfu·g-1 FM) | <2.00 |
霉菌 Molds (log10 cfu·g-1 FM) | <2.00 |
大肠杆菌 Coliform bacteria (log10 cfu·g-1 FM) | <2.00 |
表1 中药渣原料特性
Table 1 Characteristics of raw materials of traditional Chinese medicine residues (mean±SD, n=3)
项目Items | 含量Content |
---|---|
干物质 Dry matter (DM,%) | 33.92±1.42 |
中性洗涤纤维 Neutral detergent fiber (NDF,%DM) | 59.57±3.05 |
酸性洗涤纤维 Acid detergent fiber (ADF,%DM) | 42.35±2.08 |
可溶性碳水化合物 Water-soluble carbohydrates (WSC,%DM) | 7.05±1.64 |
粗蛋白质 Crude protein (CP,%DM) | 7.43±0.62 |
乳酸菌 Lactic acid bacteria (LAB,log10 cfu·g-1 FM) | <2.00 |
酵母菌 Yeast (log10 cfu·g-1 FM) | <2.00 |
霉菌 Molds (log10 cfu·g-1 FM) | <2.00 |
大肠杆菌 Coliform bacteria (log10 cfu·g-1 FM) | <2.00 |
项目 Items | 处理 Treatments | 青贮天数Silage days (D, d) | 平均值 Mean | 标准误 SEM | 显著性 Significance | |||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 7 | 15 | 34 | D | T | D×T | ||||
干物质 Dry matter (DM,%) | CK | 32.12AB | 32.42AB | 33.38A | 32.71A | 32.66 | 0.23 | NS | ** | NS |
LP+CE | 33.54A | 33.79A | 33.35A | 33.65A | 33.58 | 0.23 | ||||
LP+PE | 31.46B | 32.53AB | 32.42A | 32.79A | 32.30 | 0.29 | ||||
LP+XY | 31.05B | 31.61B | 30.89B | 29.52B | 30.77 | 0.35 | ||||
pH | CK | 5.35aA | 4.65bA | 4.71bA | 4.20cA | 4.73 | 0.13 | ** | ** | ** |
LP+CE | 4.06aC | 3.86bBC | 3.87bB | 3.73cBC | 3.88 | 0.04 | ||||
LP+PE | 3.86aD | 3.70cC | 3.74bB | 3.65dC | 3.74 | 0.02 | ||||
LP+XY | 4.14aB | 3.91bB | 3.89bB | 3.80cB | 3.93 | 0.04 | ||||
乳酸菌 Lactic acid bacteria (LAB,log10 cfu·g-1 FM) | CK | 6.86bC | 8.41aB | 8.17aA | 8.48aA | 7.98 | 0.20 | ** | ** | ** |
LP+CE | 8.48aB | 8.32aBC | 7.47bB | 5.93cB | 7.48 | 0.33 | ||||
LP+PE | 8.61aAB | 8.52aA | 7.12bB | 5.54cB | 7.62 | 0.37 | ||||
LP+XY | 8.74aA | 8.23aC | 7.21bB | 6.02cB | 7.55 | 0.33 | ||||
酵母菌 Yeast (log10 cfu·g-1 FM) | CK | <2.00 | 4.19 | 4.63 | 3.51 | 4.11 | 0.25 | NS | NS | NS |
LP+CE | <2.00 | 3.15 | 3.55 | 4.11 | 3.66 | 0.19 | ||||
LP+PE | <2.00 | <2.00 | 3.38 | 3.82 | 3.60 | 0.42 | ||||
LP+XY | <2.00 | 2.77b | 3.99a | 4.61a | 3.92 | 0.31 | ||||
霉菌 Molds (log10 cfu·g-1 FM) | CK | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ND | ND | ND |
LP+CE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+PE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+XY | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
大肠杆菌 Coliform bacteria (log10 cfu·g-1 FM) | CK | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ND | ND | ND |
LP+CE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+PE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+XY | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
乳酸 Lactic acid (LA,%DM) | CK | ND | 0.27 | 0.77 | 0.65 | 0.57 | 0.18 | NS | NS | NS |
LP+CE | 0.45 | 1.31 | 0.96 | 0.36 | 0.77 | 0.19 | ||||
LP+PE | 0.58 | 0.56 | 0.39 | 0.63 | 0.54 | 0.09 | ||||
LP+XY | 0.85 | 0.55 | 0.12 | 0.77 | 0.57 | 0.18 | ||||
乙酸 Acetic acid (AA,%DM) | CK | ND | 0.27 | 0.16AB | 0.33 | 0.26 | 0.09 | NS | NS | NS |
LP+CE | 0.15 | 0.29 | 0.24A | 0.10 | 0.20 | 0.05 | ||||
LP+PE | 0.14 | 0.13 | 0.10AB | 0.17 | 0.14 | 0.02 | ||||
LP+XY | 0.20 | 0.13 | 0.03B | 0.21 | 0.14 | 0.05 |
表2 添加植物乳杆菌和3种不同酶对中药渣青贮发酵品质的影响
Table 2 Effects of adding L. plantarum (LP) and three different enzymes on fermentation quality of traditional Chinese medicine residue silage
项目 Items | 处理 Treatments | 青贮天数Silage days (D, d) | 平均值 Mean | 标准误 SEM | 显著性 Significance | |||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 7 | 15 | 34 | D | T | D×T | ||||
干物质 Dry matter (DM,%) | CK | 32.12AB | 32.42AB | 33.38A | 32.71A | 32.66 | 0.23 | NS | ** | NS |
LP+CE | 33.54A | 33.79A | 33.35A | 33.65A | 33.58 | 0.23 | ||||
LP+PE | 31.46B | 32.53AB | 32.42A | 32.79A | 32.30 | 0.29 | ||||
LP+XY | 31.05B | 31.61B | 30.89B | 29.52B | 30.77 | 0.35 | ||||
pH | CK | 5.35aA | 4.65bA | 4.71bA | 4.20cA | 4.73 | 0.13 | ** | ** | ** |
LP+CE | 4.06aC | 3.86bBC | 3.87bB | 3.73cBC | 3.88 | 0.04 | ||||
LP+PE | 3.86aD | 3.70cC | 3.74bB | 3.65dC | 3.74 | 0.02 | ||||
LP+XY | 4.14aB | 3.91bB | 3.89bB | 3.80cB | 3.93 | 0.04 | ||||
乳酸菌 Lactic acid bacteria (LAB,log10 cfu·g-1 FM) | CK | 6.86bC | 8.41aB | 8.17aA | 8.48aA | 7.98 | 0.20 | ** | ** | ** |
LP+CE | 8.48aB | 8.32aBC | 7.47bB | 5.93cB | 7.48 | 0.33 | ||||
LP+PE | 8.61aAB | 8.52aA | 7.12bB | 5.54cB | 7.62 | 0.37 | ||||
LP+XY | 8.74aA | 8.23aC | 7.21bB | 6.02cB | 7.55 | 0.33 | ||||
酵母菌 Yeast (log10 cfu·g-1 FM) | CK | <2.00 | 4.19 | 4.63 | 3.51 | 4.11 | 0.25 | NS | NS | NS |
LP+CE | <2.00 | 3.15 | 3.55 | 4.11 | 3.66 | 0.19 | ||||
LP+PE | <2.00 | <2.00 | 3.38 | 3.82 | 3.60 | 0.42 | ||||
LP+XY | <2.00 | 2.77b | 3.99a | 4.61a | 3.92 | 0.31 | ||||
霉菌 Molds (log10 cfu·g-1 FM) | CK | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ND | ND | ND |
LP+CE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+PE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+XY | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
大肠杆菌 Coliform bacteria (log10 cfu·g-1 FM) | CK | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ND | ND | ND |
LP+CE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+PE | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
LP+XY | <2.00 | <2.00 | <2.00 | <2.00 | ND | ND | ||||
乳酸 Lactic acid (LA,%DM) | CK | ND | 0.27 | 0.77 | 0.65 | 0.57 | 0.18 | NS | NS | NS |
LP+CE | 0.45 | 1.31 | 0.96 | 0.36 | 0.77 | 0.19 | ||||
LP+PE | 0.58 | 0.56 | 0.39 | 0.63 | 0.54 | 0.09 | ||||
LP+XY | 0.85 | 0.55 | 0.12 | 0.77 | 0.57 | 0.18 | ||||
乙酸 Acetic acid (AA,%DM) | CK | ND | 0.27 | 0.16AB | 0.33 | 0.26 | 0.09 | NS | NS | NS |
LP+CE | 0.15 | 0.29 | 0.24A | 0.10 | 0.20 | 0.05 | ||||
LP+PE | 0.14 | 0.13 | 0.10AB | 0.17 | 0.14 | 0.02 | ||||
LP+XY | 0.20 | 0.13 | 0.03B | 0.21 | 0.14 | 0.05 |
项目 Items | 处理 Treatments | 青贮天数Silage days (D, d) | 平均值 Mean | 标准误 SEM | 显著性 Significance | |||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 7 | 15 | 34 | D | T | D×T | ||||
中性洗涤纤维 Neutral detergent fiber (NDF,%DM) | CK | 60.75A | 61.11A | 62.93A | 62.54A | 61.83 | 0.54 | NS | ** | NS |
LP+CE | 53.28B | 52.04B | 50.21C | 52.17C | 51.93 | 0.66 | ||||
LP+PE | 54.63B | 54.59B | 54.05BC | 52.85BC | 54.03 | 0.52 | ||||
LP+XY | 57.11AB | 57.97A | 55.51B | 56.31B | 56.72 | 0.57 | ||||
酸性洗涤纤维 Acid detergent fiber (ADF,%DM) | CK | 45.20 | 44.30 | 48.15A | 47.10A | 46.19 | 0.70 | NS | ** | NS |
LP+CE | 41.21 | 41.30 | 39.56B | 41.84B | 40.98 | 0.59 | ||||
LP+PE | 42.70 | 43.27 | 44.00AB | 42.99B | 43.24 | 0.48 | ||||
LP+XY | 42.55 | 44.13 | 42.97B | 44.66AB | 43.58 | 0.51 | ||||
可溶性碳水化合物 Water-soluble carbohydrates (WSC,%DM) | CK | 5.36aB | 5.88aC | 3.67bC | 2.61bC | 4.54 | 0.42 | * | ** | ** |
LP+CE | 12.83bA | 12.04bA | 16.91aA | 14.54abA | 14.08 | 0.74 | ||||
LP+PE | 11.72aA | 11.03abA | 11.27aB | 9.80bB | 10.96 | 0.27 | ||||
LP+XY | 8.42bcB | 7.57cB | 10.33aB | 9.62abB | 8.98 | 0.39 | ||||
粗蛋白 Crude protein (CP,%DM) | CK | 7.47B | 8.00B | 7.64B | 7.60B | 7.68 | 0.15 | NS | ** | NS |
LP+CE | 9.29A | 9.10A | 9.09A | 9.13A | 9.15 | 0.21 | ||||
LP+PE | 7.82AB | 7.49B | 8.26AB | 7.75B | 7.83 | 0.18 | ||||
LP+XY | 6.98B | 7.14B | 7.43B | 7.42B | 7.24 | 0.08 | ||||
氨态氮 Ammonium nitrogen (NH3-N,%TN) | CK | ND | 0.69 | 0.96 | 1.29B | 0.98 | 0.18 | ** | ** | * |
LP+CE | 0.37b | 0.81ab | 0.59ab | 1.06aB | 0.67 | 0.12 | ||||
LP+PE | 0.25b | 0.56ab | 0.52ab | 0.95aB | 0.57 | 0.10 | ||||
LP+XY | 0.29b | 0.80b | 0.98b | 2.44aA | 1.13 | 0.26 |
表3 添加植物乳杆菌和3种不同酶对中药渣青贮纤维和蛋白含量的影响
Table 3 Effects of adding L. plantarum (LP) and three different enzymes on fiber and protein content of traditional Chinese medicine residue silage
项目 Items | 处理 Treatments | 青贮天数Silage days (D, d) | 平均值 Mean | 标准误 SEM | 显著性 Significance | |||||
---|---|---|---|---|---|---|---|---|---|---|
3 | 7 | 15 | 34 | D | T | D×T | ||||
中性洗涤纤维 Neutral detergent fiber (NDF,%DM) | CK | 60.75A | 61.11A | 62.93A | 62.54A | 61.83 | 0.54 | NS | ** | NS |
LP+CE | 53.28B | 52.04B | 50.21C | 52.17C | 51.93 | 0.66 | ||||
LP+PE | 54.63B | 54.59B | 54.05BC | 52.85BC | 54.03 | 0.52 | ||||
LP+XY | 57.11AB | 57.97A | 55.51B | 56.31B | 56.72 | 0.57 | ||||
酸性洗涤纤维 Acid detergent fiber (ADF,%DM) | CK | 45.20 | 44.30 | 48.15A | 47.10A | 46.19 | 0.70 | NS | ** | NS |
LP+CE | 41.21 | 41.30 | 39.56B | 41.84B | 40.98 | 0.59 | ||||
LP+PE | 42.70 | 43.27 | 44.00AB | 42.99B | 43.24 | 0.48 | ||||
LP+XY | 42.55 | 44.13 | 42.97B | 44.66AB | 43.58 | 0.51 | ||||
可溶性碳水化合物 Water-soluble carbohydrates (WSC,%DM) | CK | 5.36aB | 5.88aC | 3.67bC | 2.61bC | 4.54 | 0.42 | * | ** | ** |
LP+CE | 12.83bA | 12.04bA | 16.91aA | 14.54abA | 14.08 | 0.74 | ||||
LP+PE | 11.72aA | 11.03abA | 11.27aB | 9.80bB | 10.96 | 0.27 | ||||
LP+XY | 8.42bcB | 7.57cB | 10.33aB | 9.62abB | 8.98 | 0.39 | ||||
粗蛋白 Crude protein (CP,%DM) | CK | 7.47B | 8.00B | 7.64B | 7.60B | 7.68 | 0.15 | NS | ** | NS |
LP+CE | 9.29A | 9.10A | 9.09A | 9.13A | 9.15 | 0.21 | ||||
LP+PE | 7.82AB | 7.49B | 8.26AB | 7.75B | 7.83 | 0.18 | ||||
LP+XY | 6.98B | 7.14B | 7.43B | 7.42B | 7.24 | 0.08 | ||||
氨态氮 Ammonium nitrogen (NH3-N,%TN) | CK | ND | 0.69 | 0.96 | 1.29B | 0.98 | 0.18 | ** | ** | * |
LP+CE | 0.37b | 0.81ab | 0.59ab | 1.06aB | 0.67 | 0.12 | ||||
LP+PE | 0.25b | 0.56ab | 0.52ab | 0.95aB | 0.57 | 0.10 | ||||
LP+XY | 0.29b | 0.80b | 0.98b | 2.44aA | 1.13 | 0.26 |
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