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草业学报 ›› 2025, Vol. 34 ›› Issue (5): 189-201.DOI: 10.11686/cyxb2024237

• 研究论文 • 上一篇    

菌酶协同发酵豆秸对湖羊生长性能、血清指标和瘤胃微生物的影响

祁帅1(), 张艳丽1, 万永杰1, 牛伟强2, 张积鑫2, 高雪3, 茆达干1()   

  1. 1.南京农业大学动物科技学院,江苏 南京 210095
    2.启东瑞鹏牧业有限公司,江苏 南通 226200
    3.启东市农业技术推广中心,江苏 南通 226200
  • 收稿日期:2024-06-18 修回日期:2024-07-29 出版日期:2025-05-20 发布日期:2025-03-20
  • 通讯作者: 茆达干
  • 作者简介:E-mail: maodagan@njau.edu.cn
    祁帅(2000-),男,山东曹县人,硕士。E-mail: 2022805094@stu.njau.edu.cn
  • 基金资助:
    江苏现代农业产业技术体系建设专项资金(JATS [2023]478);启东市科技项目(现代农业)(2022-3)

Effects of soybean straw co-fermented with a bacterium-enzyme mixture on the growth performance, serum indexes, and rumen microorganisms of Hu sheep

Shuai QI1(), Yan-li ZHANG1, Yong-jie WAN1, Wei-qiang NIU2, Ji-xin ZHANG2, Xue GAO3, Da-gan MAO1()   

  1. 1.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
    2.Qidong Ruipeng Animal Husbandry Co. ,Ltd. ,Nantong 226200,China
    3.Qidong Agricultural Technology Promotion Center,Nantong 226200,China
  • Received:2024-06-18 Revised:2024-07-29 Online:2025-05-20 Published:2025-03-20
  • Contact: Da-gan MAO

摘要:

本试验旨在探究菌酶协同发酵豆秸对湖羊生长性能、血清指标和瘤胃微生物的影响。48只4月龄健康湖羊公羔[(27.00±2.00) kg]随机分为4组(n=12),每组3个重复,每个重复(栏)4只羊,对照组饲喂基础日粮,试验组1、2、3分别以33%、66%、100%的发酵豆秸替代饲料中原有的豆秸,预饲期7 d,正式期60 d。结果表明:与对照组相比,1~30 d和1~60 d试验2组的平均日采食量和料重比均显著降低(P<0.05);试验3组的血清白蛋白、谷丙转氨酶、高密度脂蛋白和总胆固醇含量均显著提高(P<0.05);试验2和3组的球蛋白含量均显著升高(P<0.05);试验各组的总蛋白和谷草转氨酶活性均显著提高(P<0.05);试验3组的超氧化物歧化酶活性显著提高(P<0.05),试验各组的过氧化氢酶和谷胱甘肽过氧化物酶活性均显著提高(P<0.05);试验各组的免疫球蛋白A含量均显著提高(P<0.05),试验2和3组免疫球蛋白G和M含量均显著提高(P<0.05);试验2组粗蛋白、中性洗涤纤维和酸性洗涤纤维表观消化率均显著升高(P<0.05);试验2组的瘤胃异丁酸和氨态氮含量显著上升(P<0.05),戊酸含量显著下降(P<0.05);在门水平上,试验2组瘤胃拟杆菌门丰度显著提升(P<0.05),厚壁菌门丰度显著下降(P<0.05);在属水平上,试验2组普雷沃氏菌科UCG-001丰度显著提高(P<0.05)。菌酶协同发酵豆秸提高了湖羊抗氧化能力、免疫力和养分表观消化率,改善了湖羊瘤胃发酵和微生物结构组成,为发酵豆秸在湖羊生产中的应用提供了科学依据。

关键词: 湖羊, 生长性能, 发酵豆秸, 菌酶协同, 瘤胃微生物

Abstract:

We investigated the effects of soybean (Glycine max) straw co-fermented with a bacterium-enzyme mixture on the growth performance, serum indexes, and rumen microorganisms of Hu sheep. Forty-eight healthy male Hu lambs [(27.00±2.00) kg] aged 4 months were randomly assigned into four groups, with three replicates in each group and four lambs per replicate (stall). Lambs in the control group were fed with a basal diet (Group C), and those in the trial groups were fed with the basal diet with 33% (Group 1), 66% (Group 2), and 100% (Group 3) of the original soybean straw substituted with fermented soybean straw. The experimental period included 7 days of pre-experiment and 60 days of formal experiment. The main results were as follows: Compared with Group C, Group 2 showed a significantly decreased average daily matter intake and feed-to-gain ratio during days 1-30 and 1-60 (P<0.05). The serum contents of albumin, glutamic pyruvic transaminase, high density lipoprotein, and total cholesterol were significantly higher in Group 3 than in the control (P<0.05). The globulin contents were significantly higher in Groups 2 and 3 (P<0.05), and the total protein content and glutamic oxaloacetic transaminase activity were significantly higher in all trial groups (P<0.05) than in the control. The activity of superoxide dismutase in serum was increased significantly in Group 3 (P<0.05), and catalase and glutathione peroxidase activities in serum were increased significantly in all trial groups (P<0.05) compared with the control. The serum immunoglobulin (Ig) A content was increased significantly in all trial groups (P<0.05), and the serum IgG and IgM contents were increased significantly in Groups 2 and 3 (P<0.05), compared with the control. Compared with the control, Group 2 significantly increased apparent digestibility of crude protein, acid detergent fiber and neutral detergent fiber (P<0.05); significantly increased rumen isobutyric acid and ammonia nitrogen (NH3-N) contents (P<0.05); and significantly decreased valeric acid content (P<0.05). In terms of the bacterial community in the rumen, at the phylum level, the abundance of rumen Bacteroidota wasincreased (P<0.05), while that of Firmicuteswas decreased (P<0.05) in Group 2 compared with the control. At the genus level, the abundance of Prevotellaceae_UCG-001 was significantly higher in Group 2 than in the control (P<0.05). In conclusion, the addition of bacterium-enzyme co-fermented soybean straw to the diet can improve the antioxidant and immune capacity, nutrient apparent digestibility, the rumen environment, and the rumen microbial community structure of Hu sheep. These results provide a scientific basis for the application of fermented soybean straw in the production of Hu sheep.

Key words: Hu sheep, growth performance, fermented soybean straw, bacterium-enzyme synergy, rumen microorganisms