不同基因型菊芋耐盐生理及其生态适应性研究

doi:10.11686/cyxb2017304

草业学报 ›› 2018, Vol. 27 ›› Issue (6): 120-127.DOI: 10.11686/cyxb2017304

不同基因型菊芋耐盐生理及其生态适应性研究

朱菊华¹, 孙星², 许斌¹, 梁婷¹, 刘明¹, 缪建³, 赵耕毛^1,*

1.南京农业大学资源与环境科学学院,江苏南京 210095;
2.滁州学院生物与食品工程学院,安徽滁州 239000;
3.南通穗邦农业科技发展有限公司,江苏海安 226633

收稿日期:2017-07-10 修回日期:2017-11-22 出版日期:2018-06-20 发布日期:2018-06-20
通讯作者: * E-mail:seawater@njau.edu.cn
作者简介:朱菊华(1989-),男,江苏大丰人,在读硕士。E-mail:zhujuhua@163.com
基金资助:
国家自然科学基金项目(31370422,31400464),江苏省农业三新工程项目(SXGC[2016]108)和江苏省有机固体废弃物资源化利用协同创新项目资助

Physiological response and ecological adaptability of different Jerusalem artichoke genotypes to salt stress

ZHU Ju-hua¹, SUN Xing², XU Bing¹, LIANG Ting¹, LIU Ming¹, MIAO Jian³, ZHAO Geng-mao^1,*

1.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2.School of Biological Science and Food Engineering, Chuzhou University, Chuzhou 239000, China;
3.Nantong Sui Bang Agricultural Science and Technology Development Co., Ltd., Haian 226633, China

Received:2017-07-10 Revised:2017-11-22 Online:2018-06-20 Published:2018-06-20
Contact: * E-mail:seawater@njau.edu.cn

摘要/Abstract

摘要： 本研究解析了菊芋耐盐生理及生态适应性的基因型差异,为菊芋抗逆品种筛选与生态建植提供了理论基础。试验以广适性品种河南品种(M1)和南菊芋1号(N1)为材料,设置低盐(100 mmol·L^-1 NaCl)和高盐(200 mmol·L^-1 NaCl)2个盐胁迫处理,观测了不同基因型菊芋品种的表观形态、抗氧化酶、内源激素等变化。结果表明:(1)盐胁迫明显抑制了菊芋苗期的生长发育,致使M1和N1品种植株矮化、根系发育受阻和干物质积累减少;(2)盐胁迫对M1品种叶绿素a(Chl-a)、叶绿素b(Chl-b)和总叶绿素(T-Chl)的合成没有影响(200 mmol·L^-1 NaCl Chl-a除外);而N1品种光合色素合成受阻;(3)低盐胁迫M1和N1品种超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著增强;高盐胁迫N1品种抗氧化酶活性明显下降,而M1品种较稳定(CAT除外);(4)低盐胁迫N1品种吲哚乙酸(IAA)含量相对稳定,但促进了赤霉素(GA₃)和脱落酸(ABA)的合成,M1品种内源激素含量均无明显变化;高盐胁迫N1品种IAA和GA₃含量明显降低,ABA无明显变化;M1品种GA₃含量增加,IAA和ABA含量相对稳定。不同基因型菊芋品种间耐盐性差异显著(M1>N1),且通过抗氧化酶活性、内源激素等内反馈调节机制,提高其生态适应性。

关键词: 菊芋, 光合色素, 抗氧化酶, 内源激素, 生态适应性

Abstract: The physiological and ecological adaptability of different genotypes of Jerusalem artichoke were analyzed to measure the responses of their morphology, antioxidant enzymes and endogenous hormones to salt stress. This study provides a theoretical basis for both varieties selection and ecological planting. Using the eurytopic varieties of Southern Jerusalem artichoke No.1 (N1) and Puyang Henan (M1) as experimental materials, two salt-stressed treatments of 100 mmol·L^-1 NaCl (low salt level) and 200 mmol·L^-1 NaCl (high salt level) were laid out in a randomized complete block design with 3 replications in each case. The results showed that: 1) Salt stress significantly inhibited seedling growth in Jerusalem artichoke, resulting in plant dwarfing, stunted root growth and a dry matter decrease of M1 and N1. 2) Salt stress did not affect the synthesis of chlorophyll-a, chlorophyll-b and total chlorophyll in M1 (except for chlorophyll-a under 200 mmol·L^-1 NaCl stress), but it blocked photosynthetic pigment synthesis in N1. 3) Low salt stress significantly increased superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities in M1 and N1, while high salt stress markedly decreased enzyme activities in N1 and had no effect on enzyme activities in M1 (except for CAT). 4) Low salt stress promoted the synthesis of GA₃ (gibberellin) and ABA (abscisic acid) in N1, but IAA (indoleacetic acid) content was relatively stable and endogenous hormone content in M1 was not obviously changed. High salt stress decreased the content of IAA and GA₃ in N1, but there was no obvious change in ABA. The content of GA₃ in M1 increased, while IAA and ABA were relatively stable. The differences in salt tolerance between the Jerusalem artichoke genotypes were significant (M1>N1), and the internal adaptation of antioxidant enzymes and endogenous hormones could improve the plant’s ecological adaptability to salt stress.

Key words: jerusalem artichoke (Helianthus tuberosus), photosynthetic pigment, enzymes, hormones, ecological adaptability

朱菊华, 孙星, 许斌, 梁婷, 刘明, 缪建, 赵耕毛. 不同基因型菊芋耐盐生理及其生态适应性研究[J]. 草业学报, 2018, 27(6): 120-127.

ZHU Ju-hua, SUN Xing, XU Bing, LIANG Ting, LIU Ming, MIAO Jian, ZHAO Geng-mao. Physiological response and ecological adaptability of different Jerusalem artichoke genotypes to salt stress[J]. Acta Prataculturae Sinica, 2018, 27(6): 120-127.

参考文献

[1] Denoroy P.The crop physiology of Helianthus tuberosus L.: a model oriented view. Biomass and Bioenergy, 1996, 11(1): 11-32.
[2] Wu R N, Zhu T X, Yu Y Q, et al. Study on status and exploiting potential of Helianthus tuberosus. Acta Prataculturae Sinica, 2013, 30(8): 1295-1300.
乌日娜, 朱铁霞, 于永奇, 等. 菊芋的研究现状及开发潜力. 草业科学, 2013, 30(8): 1295-1300.
[3] Abdalla N, Domokos-Szabolcsy E, El-Ramady H, et al. Jerusalem artichoke (Helianthus tuberosus L.): a review of in vivo and in vitro propagation. International Journal of Horticultural Science, 2014, 20(3/4): 131-136.
[4] Liu Z P, Long X H, Lu L, et al. The study of bio-energy plants development from non-tillage resource of Coastal Mudflat. Journal of natural resources, 2008, 23(1): 9-14.
刘兆普, 隆小华, 刘玲, 等. 海岸带滨海盐土资源发展能源植物资源的研究.自然资源学报, 2008, 23(1): 9-14.
[5] Zhao G M, Mehta S K, Liu Z P.Use of saline aquaculture wastewater to irrigate salt-tolerant jerusalem artichoke and sunflower in semiarid coastal zones of China. Agricultural Water Management, 2010, 97: 1987-1993.
[6] Zhang G M, Liu Z P, Cheng M D, et al. Effect of saline aquaculture effluent on salt tolerant jerusalem artichoke (Helianthus tuberosus L.) in a semi-arid coastal area of China. Pedosphere, 2006,16: 762-769.
[7] Wu C L, Yin J L, Xu Y C, et al. Effects of alkaline stress on growth, photosynthesis and antioxidation of Helianthus tuberosus seedlings. Acta Bot Boreali-Occident Sin, 2006, 26(30): 447-454.
吴成龙, 尹金来, 徐阳春. 碱胁迫对菊芋幼苗生长及其光合作用和抗氧化作用的影响. 西北植物学报, 2006, 26(3): 447-454.
[8] Long X H, Liu Z P, Zhen Q S, et al. Effects of seawater with different concentrations on growth and physiological and biochemical characteristics of Helianthus tuberosus seedlings. Acta Ecologica Sinica, 2005, 25(8): 1881-1890.
隆小华, 刘兆普, 郑青松, 等. 不同浓度海水对菊芋幼苗生长及生理生化特性的影响. 生态学报, 2005, 25(8): 1881-1890.
[9] Wang L, Ling X H, Hao L X, et al. Effects of nitrogen form on the photochemical efficiency of PSⅡ and antioxidant characteristics of Jerusalem artichoke seeding under salt stress. Acta Prataculturae Sinica, 2012, 21(1): 133-140.
王磊, 隆小华, 郝连香, 等. 氮素形态对盐胁迫下菊芋幼苗PSⅡ光化学效率及抗氧化特性的影响. 草业学报, 2012, 21(1): 133-140.
[10] Rhoades J D, Kandiah A, Mashali A M.The use of saline waters for crop production. Rome: Food and Agriculture Organization of the United Nations, 1992.
[11] Newton P J, Myers B A, West D W.Reduction in growth and yield of jerusalem artichoke caused by soil salinity. Journal of Irrigation and Drainage Engineering-asce, 1991, 12: 213-222.
[12] Maas E V, Grattan S R.Crop yields as affected by salinity//Skaggs R W, van Schilfgaarde. Agriculture drainage. Agronomy Monograph. WI: ASA, CSSA, SSSA, Madison, 1999, 38: 55-108.
[13] Cakmak I, Horst W J.Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 2006, 83(3): 463-468.
[14] Giannopolitis C N, Ries S K.Superoxide dismutase: I. occurrence in higher plants. Plant Physiology, 1977, 59(2): 309-314.
[15] Chance B, Maehly A C.Assay of catalases and peroxidases. Methods in Enzymology, 1955, 2(55): 764-775.
[16] Bergmeyer H U, Gawehn K, Grassl M.Methods of enzymatic analysis. In: Bergmeyer H U, Ed. Verlag Chemie, Wienheim, 1974, Vol.1, 481-482.
[17] Ge L, Yong J W H, Tan S N, et al. Analysis of some cytokinins in coconut (Cocos nucifera L.) water by micellar electrokinetic capillary chromatography after solid-phase extraction. Journal of Chromatography A, 2004, 1048(1): 119-126.
[18] Hou S, Zhu J, Ding M, et al. Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry. Talanta, 2008, 76(4): 798-802.
[19] Datta J K, Nag S, Banerjee A, et al. Impact of salt stress on five varieties of wheat (Triticum aestivum L.) cultivars under laboratory condition. Journal of Applied Sciences and Environmental Management, 2009, 13(3): 93-97.
[20] Dolatabadian A, Sanavy S A M M, Ghanati F. Effect of salinity on growth, xylem structure and anatomical characteristics of soybean. Notulae Scientia Biologicae, 2011, 3(1): 41-45.
[21] Tunçtürk M, Tunçtürk R, Yildirim B, et al. Effect of salinity stress on plant fresh weight and nutrient composition of some Canola (Brassica napus L.) cultivars. African Journal of Biotechnology, 2013, 10(10): 1827-1832.
[22] Akhzari D A, Pessarakli M.Studying the effects of salinity stress on the growth of various halophytic plant species (Agropyron elongatum, Kochia prostrata and Puccinellia distans). World Applied Sciences Journal, 2012, 16: 998-1003.
[23] Demmigadams A B, Iii W.Photoprotection and other responses of plants to high light stress. Annual Review of Plant Biology, 1992, 43(1): 599-626.
[24] Chen J M, Zheng Q S, Liu Z P, et al. Growing and photosynthetic response of Jatropha curcas L. seedlings to salt stress. Acta Ecologica Sinica, 2009, 29(3): 1356-1365.
陈健妙, 郑青松, 刘兆普, 等. 麻疯树(Jatropha curcas L.)幼苗生长和光合作用对盐胁迫的响应. 生态学报, 2009, 29(3): 1356-1365.
[25] Xue Y F, Liu Z P.A Preliminary study of screening salt-resistive varieties of Helianthus tuberosus L. using chlorophyll fluorescence parameters. Chinese High Technology Letters, 2008, 18(7): 766-770.
薛延丰, 刘兆普. 利用叶绿素荧光参数筛选抗盐菊芋品种的初步研究. 高技术通讯, 2008, 18(7): 766-770.
[26] Moharekar S, Lokhande S, Hara T, et al. Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings. Photosynthetica, 2003, 41(2): 315-317.
[27] Breusegem F V, Vranová E, Dat J F, et al. The role of active oxygen species in plant signal transduction. Plant Science, 2001, 161(3): 405-414.
[28] Meloni D A, Oliva M A, Martinez C A, et al. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany, 2003, 49(1): 69-76.
[29] Cachorro P, Martínez R, Ortiz A, et al. Abscisic acid and osmotic relations in Phaseolus vulgaris L. shoots under salt stress. Journal of Plant Growth Regulation, 1995, 14(2): 99-104.

不同基因型菊芋耐盐生理及其生态适应性研究

Physiological response and ecological adaptability of different Jerusalem artichoke genotypes to salt stress

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	覃凤飞, 李志华, 刘信宝, 渠晖, 平措卓玛, 洛松群措, 苏梦涵. 外源2,4表油菜素内酯对越夏期高温与弱光胁迫下紫花苜蓿生长和光合性能的影响[J]. 草业学报, 2020, 29(9): 146-160.
[2]	张利霞, 常青山, 薛娴, 刘伟, 张巧明, 陈苏丹, 郑轶琦, 李景林, 陈婉东, 李大钊. 酸胁迫对夏枯草叶绿素荧光特性和根系抗氧化酶活性的影响[J]. 草业学报, 2020, 29(8): 134-142.
[3]	刘燕, 于美玲, 张然, 牛奎举, 李玉珠, 张金青, 马晖玲. 甘肃野生草地早熟禾内源激素含量的变化与无融合生殖率的关系研究[J]. 草业学报, 2020, 29(7): 99-111.
[4]	赵颖, 魏小红, 李桃桃. 外源NO对混合盐碱胁迫下藜麦种子萌发和幼苗生长的影响[J]. 草业学报, 2020, 29(4): 92-101.
[5]	鲍根生, 宋梅玲, 王玉琴, 刘静, 王宏生. 不同密度甘肃马先蒿寄生和内生真菌互作对紫花针茅内源激素及生物碱含量的影响[J]. 草业学报, 2020, 29(4): 147-156.
[6]	衣琨, 赵一航, 胡尧, 刘佳雪, 贺涛涛, 李旭, 宋鹏, 崔国文, 殷秀杰. GA₃和6-BA对高加索三叶草根蘖芽生长及内源激素含量的影响[J]. 草业学报, 2020, 29(2): 22-30.
[7]	王宁, 付亚军, 袁美丽, 刘征阳, 张铭鑫, 米银法. GA₃浸种对入侵植物节节麦种子破眠及发芽特性的影响[J]. 草业学报, 2020, 29(2): 73-81.
[8]	许爱云, 曹兵, 谢云. 干旱风沙区煤炭基地12种草本植物对干旱胁迫的生理生态响应及抗旱性评价[J]. 草业学报, 2020, 29(10): 22-34.
[9]	张翔, 杨勇, 刘学勇, 向佐湘. 外源水杨酸对低温胁迫下海滨雀稗抗寒生理特征的影响[J]. 草业学报, 2020, 29(1): 117-124.
[10]	伍国强, 李辉, 雷彩荣, 蔺丽媛, 金娟, 李善家. 添加KCl对高盐胁迫下红豆草生长及生理特性的影响[J]. 草业学报, 2019, 28(6): 45-55.
[11]	项洪涛, 齐德强, 李琬, 郑殿峰, 王月溪, 王彤彤, 王立志, 曾宪楠, 杨纯杰, 周行, 赵海东. 低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响[J]. 草业学报, 2019, 28(4): 81-94.
[12]	赵颖, 魏小红, 赫亚龙, 赵枭飞, 韩厅, 岳凯, 辛夏青, 宿梅飞, 马文静, 骆巧娟. 混合盐碱胁迫对藜麦种子萌发和幼苗抗氧化特性的影响[J]. 草业学报, 2019, 28(2): 156-167.
[13]	刘攀道, 罗佳佳, 白昌军, 陈志坚, 刘国道. 过量锰处理对苗期柱花草生长及抗氧化酶活性的影响[J]. 草业学报, 2018, 27(9): 194-200.
[14]	赵颖, 弋钦, 魏小红, 辛夏青, 韩厅, 岳凯, 王方琳. NO介导的Ca²⁺信号对渗透胁迫下紫花苜蓿幼苗光合特征及抗性的影响[J]. 草业学报, 2018, 27(5): 130-140.
[15]	黄钰芳, 张恩和, 张新慧, 王惠珍, 王琦, 刘青林, 石雨仟. 兰州百合连作障碍效应及机制研究[J]. 草业学报, 2018, 27(2): 146-155.