草业学报 ›› 2021, Vol. 30 ›› Issue (11): 144-156.DOI: 10.11686/cyxb2020429
• 研究论文 • 上一篇
收稿日期:
2020-09-22
修回日期:
2020-11-02
出版日期:
2021-10-19
发布日期:
2021-10-19
通讯作者:
张吉宇
作者简介:
Corresponding author. E-mail: zhangjy@lzu.edu.cn基金资助:
Qian MA(), Qi YAN, Zheng-she ZHANG, Fan WU, Ji-yu ZHANG()
Received:
2020-09-22
Revised:
2020-11-02
Online:
2021-10-19
Published:
2021-10-19
Contact:
Ji-yu ZHANG
摘要:
紫花苜蓿是全世界广泛栽培的优质牧草。木质素与紫花苜蓿抗性密切相关,但木质素难以被牲畜消化,严重影响了紫花苜蓿的营养价值,所以培育低木质素的紫花苜蓿具有重要意义。咖啡酰辅酶A氧甲基转移酶(CCoAOMT)是木质素合成途径中的关键酶,本研究利用生物信息学方法对紫花苜蓿基因组中的CCoAOMT基因家族成员进行鉴定,并对其基因结构、染色体定位、系统发育、基因表达等方面进行了研究。结果表明,在紫花苜蓿基因组中共鉴定到44个MsCCoAOMT基因,其中36个(82%)MsCCoAOMT基因都含有5个外显子,分布于16条染色体上,存在明显的串联重复现象;通过系统发育树分析,MsCCoAOMT基因家族可分为5类;通过MEME软件对MsCCoAOMT蛋白motif进行预测发现了10个比较保守的motif。qRT-PCR表达分析表明部分MsCCoAOMT基因的表达具有组织特异性。研究结果对紫花苜蓿低木质素遗传改良具有潜在的理论指导意义和实际参考价值。
马倩, 闫启, 张正社, 吴凡, 张吉宇. 紫花苜蓿CCoAOMT基因家族的鉴定、进化及表达分析[J]. 草业学报, 2021, 30(11): 144-156.
Qian MA, Qi YAN, Zheng-she ZHANG, Fan WU, Ji-yu ZHANG. Identification, evolution and expression analysis of the CCoAOMT family genes in Medicago sativa[J]. Acta Prataculturae Sinica, 2021, 30(11): 144-156.
基因名称 Gene name | 引物序列 Primer sequence (5'-3') | 基因名称 Gene name | 引物序列 Primer sequence (5'-3') |
---|---|---|---|
MsCCoAOMT1 | F: TGCTGATCCATTGGTAAGTGAC | MsCCoAOMT25 | F: CTATACCCGGTGATGAAGGAC |
R: TTGTCATCCCATCTCCAATAGGT | R: CAAAGCCAATACCTTTCCATCAG | ||
MsCCoAOMT10 | F: TTCATGGGCACTTCACCT | MsCCoAOMT32 | F: CTATACCCGCTGATGAAGGAC |
R: GGCTGTAATCTTTCCATCATATGG | R: AGCCAACACCTTTCCATCCT | ||
MsCCoAOMT12 | F: AAGCAAACTGAATCTGGAAGAC | MsCCoAOMT41 | F: GGAACCTTATGGCTACACCT |
R: TTGTCATGATGTTCCATGGGT | R: AAGCCAATACCTTTCCATCAG | ||
MsCCoAOMT17 | F: TGCCTTTGGATGAAGCAC | MsCCoAOMT43 | F: CACCCATGCTTGAAGGAG |
R: CAATTCCTATTACCTTGCCATCAG | R: AGCTAACACCTTTCCATCCT | ||
MsCCoAOMT21 | F: GGCTGGTCAACTAATCACTC | MsCCoAOMT44 | F: CTAATGGGCATACTCTTGAAGCT |
R: GGCTGTAATCTTTCCATCGT | R: AGCTATGATCTTTCCATCATCAGG | ||
MsCCoAOMT23 | F: GAGATGACACTATCACACCCT | MsActin | F: GACAATGGAACTGGAATGG |
R: AAGCCAATACCTTTCCATCAG | R: CAATACCGTGCTCAATGG |
表1 qRT-PCR 引物信息
Table 1 Primers used for qRT-PCR analysis
基因名称 Gene name | 引物序列 Primer sequence (5'-3') | 基因名称 Gene name | 引物序列 Primer sequence (5'-3') |
---|---|---|---|
MsCCoAOMT1 | F: TGCTGATCCATTGGTAAGTGAC | MsCCoAOMT25 | F: CTATACCCGGTGATGAAGGAC |
R: TTGTCATCCCATCTCCAATAGGT | R: CAAAGCCAATACCTTTCCATCAG | ||
MsCCoAOMT10 | F: TTCATGGGCACTTCACCT | MsCCoAOMT32 | F: CTATACCCGCTGATGAAGGAC |
R: GGCTGTAATCTTTCCATCATATGG | R: AGCCAACACCTTTCCATCCT | ||
MsCCoAOMT12 | F: AAGCAAACTGAATCTGGAAGAC | MsCCoAOMT41 | F: GGAACCTTATGGCTACACCT |
R: TTGTCATGATGTTCCATGGGT | R: AAGCCAATACCTTTCCATCAG | ||
MsCCoAOMT17 | F: TGCCTTTGGATGAAGCAC | MsCCoAOMT43 | F: CACCCATGCTTGAAGGAG |
R: CAATTCCTATTACCTTGCCATCAG | R: AGCTAACACCTTTCCATCCT | ||
MsCCoAOMT21 | F: GGCTGGTCAACTAATCACTC | MsCCoAOMT44 | F: CTAATGGGCATACTCTTGAAGCT |
R: GGCTGTAATCTTTCCATCGT | R: AGCTATGATCTTTCCATCATCAGG | ||
MsCCoAOMT23 | F: GAGATGACACTATCACACCCT | MsActin | F: GACAATGGAACTGGAATGG |
R: AAGCCAATACCTTTCCATCAG | R: CAATACCGTGCTCAATGG |
基因名称 Gene name | 基因座 Gene ID | 蛋白质大小 Protein length (aa) | 分子量MW (kDa) | 等电点 pI | 疏水性指数 GRAVY | 基因序列长度Length of intron and exon (bp) | 亚细胞定位Subcellular localization | 拟南芥同源基因 Homologous gene of Arabidopsis |
---|---|---|---|---|---|---|---|---|
MsCCoAOMT1 | MS.gene039184.t1 | 309 | 34.3566 | 8.54 | -0.123 | 5315 | Cytoplasm | AT3G62000.1 |
MsCCoAOMT2 | MS.gene068675.t1 | 293 | 32.6967 | 8.58 | -0.083 | 3724 | Chloroplast | AT3G62000.1 |
MsCCoAOMT3 | MS.gene063440.t1 | 293 | 32.7298 | 8.58 | -0.061 | 3345 | Chloroplast | AT3G62000.1 |
MsCCoAOMT4 | MS.gene062840.t1 | 267 | 29.9507 | 8.58 | -0.080 | 7396 | Chloroplast | AT3G62000.1 |
MsCCoAOMT5 | MS.gene049025.t1 | 251 | 27.9613 | 5.51 | 0.020 | 1473 | Chloroplast | AT4G26220.1 |
MsCCoAOMT6 | MS.gene26463.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1302 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT7 | MS.gene06173.t1 | 235 | 26.1382 | 5.51 | -0.023 | 1495 | Chloroplast | AT4G26220.1 |
MsCCoAOMT8 | MS.gene058635.t1 | 133 | 14.8534 | 5.59 | 0.041 | 724 | N and C | AT1G67980.1 |
MsCCoAOMT9 | MS.gene028389.t1 | 229 | 25.7237 | 5.14 | -0.114 | 2386 | Chloroplast | AT1G67980.1 |
MsCCoAOMT10 | MS.gene55886.t1 | 235 | 26.0962 | 5.51 | -0.043 | 1495 | Chloroplast | AT4G26220.1 |
MsCCoAOMT11 | MS.gene28756.t1 | 229 | 25.7418 | 5.23 | -0.097 | 1842 | Chloroplast | AT1G67980.1 |
MsCCoAOMT12 | MS.gene059423.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1303 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT13 | MS.gene053535.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1303 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT14 | MS.gene053539.t1 | 248 | 28.0291 | 5.41 | -0.280 | 1303 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT15 | MS.gene053537.t1 | 182 | 20.5040 | 6.31 | -0.066 | 881 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT16 | MS.gene20157.t1 | 235 | 26.1222 | 5.51 | -0.023 | 1492 | Chloroplast | AT4G26220.1 |
MsCCoAOMT17 | MS.gene35069.t1 | 229 | 25.7377 | 5.14 | -0.112 | 2454 | Chloroplast | AT1G67980.1 |
MsCCoAOMT18 | MS.gene047856.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1322 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT19 | MS.gene064029.t1 | 238 | 26.8580 | 5.23 | -0.174 | 2072 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT20 | MS.gene030303.t1 | 238 | 26.8720 | 5.35 | -0.175 | 2087 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT21 | MS.gene75055.t1 | 238 | 26.9041 | 5.35 | -0.182 | 2042 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT22 | MS.gene66349.t1 | 238 | 26.8580 | 5.23 | -0.174 | 2072 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT23 | MS.gene44534.t1 | 238 | 26.7568 | 5.58 | -0.132 | 1866 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT24 | MS.gene44535.t1 | 568 | 63.5446 | 4.89 | -0.170 | 4423 | Chloroplast | AT2G23250.1 |
MsCCoAOMT25 | MS.gene011784.t1 | 248 | 27.9000 | 5.15 | -0.173 | 3149 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT26 | MS.gene011803.t1 | 248 | 27.8408 | 4.99 | -0.198 | 3883 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT27 | MS.gene011804.t1 | 246 | 27.7320 | 5.50 | -0.130 | 1624 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT28 | MS.gene30425.t1 | 187 | 20.9131 | 5.22 | -0.100 | 2679 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT29 | MS.gene011778.t1 | 238 | 26.7828 | 5.48 | -0.124 | 2777 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT30 | MS.gene56811.t1 | 248 | 27.8449 | 5.06 | -0.158 | 4031 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT31 | MS.gene56793.t1 | 248 | 27.8689 | 4.99 | -0.185 | 3744 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT32 | MS.gene56792.t1 | 189 | 21.6996 | 5.65 | 0.286 | 1330 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT33 | MS.gene074442.t1 | 235 | 26.3723 | 5.52 | -0.197 | 3568 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT34 | MS.gene90469.t1 | 238 | 26.7828 | 5.48 | -0.124 | 1850 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT35 | MS.gene90479.t1 | 248 | 27.9000 | 5.15 | -0.173 | 2947 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT36 | MS.gene90498.t1 | 248 | 27.8127 | 4.91 | -0.182 | 3043 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT37 | MS.gene90499.t1 | 246 | 27.7240 | 5.50 | -0.108 | 1637 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT38 | MS.gene051092.t1 | 235 | 26.4173 | 5.30 | -0.186 | 3565 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT39 | MS.gene041940.t1 | 238 | 26.7828 | 5.48 | -0.124 | 2498 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT40 | MS.gene041950.t1 | 248 | 27.9071 | 5.16 | -0.160 | 2984 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT41 | MS.gene99216.t1 | 248 | 27.8408 | 4.99 | -0.195 | 3776 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT42 | MS.gene99215.t1 | 246 | 27.7351 | 5.50 | -0.034 | 1458 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT43 | MS.gene99213.t1 | 246 | 27.7460 | 5.50 | -0.129 | 1637 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT44 | MS.gene25100.t1 | 235 | 26.4394 | 5.55 | -0.185 | 3584 | Cytoplasm | AT4G26220.1 |
表2 紫花苜蓿CCoAOMT基因家族信息
Table 2 The information of CCoAOMT gene family in M. sativa
基因名称 Gene name | 基因座 Gene ID | 蛋白质大小 Protein length (aa) | 分子量MW (kDa) | 等电点 pI | 疏水性指数 GRAVY | 基因序列长度Length of intron and exon (bp) | 亚细胞定位Subcellular localization | 拟南芥同源基因 Homologous gene of Arabidopsis |
---|---|---|---|---|---|---|---|---|
MsCCoAOMT1 | MS.gene039184.t1 | 309 | 34.3566 | 8.54 | -0.123 | 5315 | Cytoplasm | AT3G62000.1 |
MsCCoAOMT2 | MS.gene068675.t1 | 293 | 32.6967 | 8.58 | -0.083 | 3724 | Chloroplast | AT3G62000.1 |
MsCCoAOMT3 | MS.gene063440.t1 | 293 | 32.7298 | 8.58 | -0.061 | 3345 | Chloroplast | AT3G62000.1 |
MsCCoAOMT4 | MS.gene062840.t1 | 267 | 29.9507 | 8.58 | -0.080 | 7396 | Chloroplast | AT3G62000.1 |
MsCCoAOMT5 | MS.gene049025.t1 | 251 | 27.9613 | 5.51 | 0.020 | 1473 | Chloroplast | AT4G26220.1 |
MsCCoAOMT6 | MS.gene26463.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1302 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT7 | MS.gene06173.t1 | 235 | 26.1382 | 5.51 | -0.023 | 1495 | Chloroplast | AT4G26220.1 |
MsCCoAOMT8 | MS.gene058635.t1 | 133 | 14.8534 | 5.59 | 0.041 | 724 | N and C | AT1G67980.1 |
MsCCoAOMT9 | MS.gene028389.t1 | 229 | 25.7237 | 5.14 | -0.114 | 2386 | Chloroplast | AT1G67980.1 |
MsCCoAOMT10 | MS.gene55886.t1 | 235 | 26.0962 | 5.51 | -0.043 | 1495 | Chloroplast | AT4G26220.1 |
MsCCoAOMT11 | MS.gene28756.t1 | 229 | 25.7418 | 5.23 | -0.097 | 1842 | Chloroplast | AT1G67980.1 |
MsCCoAOMT12 | MS.gene059423.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1303 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT13 | MS.gene053535.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1303 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT14 | MS.gene053539.t1 | 248 | 28.0291 | 5.41 | -0.280 | 1303 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT15 | MS.gene053537.t1 | 182 | 20.5040 | 6.31 | -0.066 | 881 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT16 | MS.gene20157.t1 | 235 | 26.1222 | 5.51 | -0.023 | 1492 | Chloroplast | AT4G26220.1 |
MsCCoAOMT17 | MS.gene35069.t1 | 229 | 25.7377 | 5.14 | -0.112 | 2454 | Chloroplast | AT1G67980.1 |
MsCCoAOMT18 | MS.gene047856.t1 | 247 | 27.9992 | 5.55 | -0.308 | 1322 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT19 | MS.gene064029.t1 | 238 | 26.8580 | 5.23 | -0.174 | 2072 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT20 | MS.gene030303.t1 | 238 | 26.8720 | 5.35 | -0.175 | 2087 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT21 | MS.gene75055.t1 | 238 | 26.9041 | 5.35 | -0.182 | 2042 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT22 | MS.gene66349.t1 | 238 | 26.8580 | 5.23 | -0.174 | 2072 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT23 | MS.gene44534.t1 | 238 | 26.7568 | 5.58 | -0.132 | 1866 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT24 | MS.gene44535.t1 | 568 | 63.5446 | 4.89 | -0.170 | 4423 | Chloroplast | AT2G23250.1 |
MsCCoAOMT25 | MS.gene011784.t1 | 248 | 27.9000 | 5.15 | -0.173 | 3149 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT26 | MS.gene011803.t1 | 248 | 27.8408 | 4.99 | -0.198 | 3883 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT27 | MS.gene011804.t1 | 246 | 27.7320 | 5.50 | -0.130 | 1624 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT28 | MS.gene30425.t1 | 187 | 20.9131 | 5.22 | -0.100 | 2679 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT29 | MS.gene011778.t1 | 238 | 26.7828 | 5.48 | -0.124 | 2777 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT30 | MS.gene56811.t1 | 248 | 27.8449 | 5.06 | -0.158 | 4031 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT31 | MS.gene56793.t1 | 248 | 27.8689 | 4.99 | -0.185 | 3744 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT32 | MS.gene56792.t1 | 189 | 21.6996 | 5.65 | 0.286 | 1330 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT33 | MS.gene074442.t1 | 235 | 26.3723 | 5.52 | -0.197 | 3568 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT34 | MS.gene90469.t1 | 238 | 26.7828 | 5.48 | -0.124 | 1850 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT35 | MS.gene90479.t1 | 248 | 27.9000 | 5.15 | -0.173 | 2947 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT36 | MS.gene90498.t1 | 248 | 27.8127 | 4.91 | -0.182 | 3043 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT37 | MS.gene90499.t1 | 246 | 27.7240 | 5.50 | -0.108 | 1637 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT38 | MS.gene051092.t1 | 235 | 26.4173 | 5.30 | -0.186 | 3565 | Cytoplasm | AT4G26220.1 |
MsCCoAOMT39 | MS.gene041940.t1 | 238 | 26.7828 | 5.48 | -0.124 | 2498 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT40 | MS.gene041950.t1 | 248 | 27.9071 | 5.16 | -0.160 | 2984 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT41 | MS.gene99216.t1 | 248 | 27.8408 | 4.99 | -0.195 | 3776 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT42 | MS.gene99215.t1 | 246 | 27.7351 | 5.50 | -0.034 | 1458 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT43 | MS.gene99213.t1 | 246 | 27.7460 | 5.50 | -0.129 | 1637 | Cytoplasm | AT4G34050.1 |
MsCCoAOMT44 | MS.gene25100.t1 | 235 | 26.4394 | 5.55 | -0.185 | 3584 | Cytoplasm | AT4G26220.1 |
图1 紫花苜蓿CCoAOMT基因的染色体定位标尺表示染色体长度,黑色箭头表示基因的转录方向,红色字体标注串联重复基因。The scale indicates the chromosome length, the black arrow indicates the transcription direction of the gene, and the red font marks the tandem repeat gene.
Fig.1 Location of MsCCoAOMT genes on chromosome
图2 紫花苜蓿CCoAOMT基因家族的保守基序及基因结构分析a. MsCCoAOMT基因家族的保守基序;b. MsCCoAOMT基因家族的基因结构,绿色框、黑色线条分别代表外显子和内含子;5个不同的背景色表示五大类。a. Conserved motifs of the MsCCoAOMT family; b. Gene structure of the MsCCoAOMT family. The green boxes and black lines represent exon and intron, respectively; Class A, B, C, D, and E are displayed in different background colors.
Fig.2 Analysis of conserved motifs and gene structure of the MsCCoAOMT gene family
基因名称 Gene name | 元件名称Element name | |||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
基本核心元件 Basic core components | 光信号感知顺式作用元件Optical signal sensing cis-acting element | 激素响应元件 Hormone response element | 生物应激反应元件 Biological stress response element | 蛋白质结合元件 Protein binding element | 组织发育元件 Tissue development element | |||||||||||||||||||||||||||
X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | X14 | X15 | X16 | X17 | X18 | X19 | X20 | X21 | X22 | X23 | X24 | X25 | X26 | X27 | X28 | X29 | X30 | X31 | X32 | |
Ms1 | 46 | 24 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms2 | 34 | 22 | 1 | 0 | 1 | 3 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms3 | 43 | 14 | 3 | 2 | 3 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms4 | 33 | 11 | 0 | 0 | 2 | 2 | 2 | 0 | 2 | 0 | 3 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 |
Ms5 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms6 | 32 | 47 | 1 | 0 | 2 | 4 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms7 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms8 | 41 | 51 | 1 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms9 | 39 | 56 | 1 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms10 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms11 | 38 | 54 | 0 | 0 | 4 | 1 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Ms12 | 31 | 43 | 1 | 0 | 2 | 3 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms13 | 33 | 47 | 1 | 0 | 2 | 3 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms14 | 23 | 75 | 0 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms15 | 43 | 36 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Ms16 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms17 | 39 | 56 | 1 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms18 | 32 | 48 | 1 | 0 | 2 | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms19 | 44 | 60 | 0 | 0 | 3 | 3 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms20 | 39 | 43 | 0 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Ms21 | 43 | 60 | 0 | 0 | 2 | 3 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms22 | 44 | 61 | 0 | 0 | 2 | 3 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms23 | 41 | 25 | 1 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms24 | 38 | 33 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 2 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms25 | 39 | 60 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Ms26 | 29 | 21 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 |
Ms27 | 39 | 53 | 0 | 0 | 4 | 2 | 1 | 1 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms28 | 53 | 46 | 1 | 1 | 5 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Ms29 | 40 | 27 | 1 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms30 | 37 | 66 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Ms31 | 39 | 56 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Ms32 | 47 | 43 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Ms33 | 46 | 28 | 0 | 0 | 2 | 3 | 2 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Ms34 | 42 | 29 | 1 | 1 | 4 | 2 | 4 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms35 | 42 | 61 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Ms36 | 33 | 64 | 0 | 1 | 3 | 1 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Ms37 | 44 | 67 | 0 | 1 | 5 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms38 | 46 | 31 | 0 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Ms39 | 42 | 26 | 1 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 1 | 1 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms40 | 29 | 43 | 1 | 1 | 7 | 2 | 2 | 1 | 1 | 0 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Ms41 | 40 | 59 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Ms42 | 44 | 61 | 0 | 1 | 4 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms43 | 44 | 65 | 0 | 1 | 4 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms44 | 48 | 23 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
表3 启动子序列顺式作用元件分析
Table 3 Analysis of promoter sequence cis-acting
基因名称 Gene name | 元件名称Element name | |||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
基本核心元件 Basic core components | 光信号感知顺式作用元件Optical signal sensing cis-acting element | 激素响应元件 Hormone response element | 生物应激反应元件 Biological stress response element | 蛋白质结合元件 Protein binding element | 组织发育元件 Tissue development element | |||||||||||||||||||||||||||
X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | X14 | X15 | X16 | X17 | X18 | X19 | X20 | X21 | X22 | X23 | X24 | X25 | X26 | X27 | X28 | X29 | X30 | X31 | X32 | |
Ms1 | 46 | 24 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms2 | 34 | 22 | 1 | 0 | 1 | 3 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms3 | 43 | 14 | 3 | 2 | 3 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms4 | 33 | 11 | 0 | 0 | 2 | 2 | 2 | 0 | 2 | 0 | 3 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 |
Ms5 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms6 | 32 | 47 | 1 | 0 | 2 | 4 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms7 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms8 | 41 | 51 | 1 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms9 | 39 | 56 | 1 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms10 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms11 | 38 | 54 | 0 | 0 | 4 | 1 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Ms12 | 31 | 43 | 1 | 0 | 2 | 3 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms13 | 33 | 47 | 1 | 0 | 2 | 3 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms14 | 23 | 75 | 0 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms15 | 43 | 36 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Ms16 | 35 | 39 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms17 | 39 | 56 | 1 | 0 | 2 | 4 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms18 | 32 | 48 | 1 | 0 | 2 | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Ms19 | 44 | 60 | 0 | 0 | 3 | 3 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms20 | 39 | 43 | 0 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Ms21 | 43 | 60 | 0 | 0 | 2 | 3 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms22 | 44 | 61 | 0 | 0 | 2 | 3 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms23 | 41 | 25 | 1 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms24 | 38 | 33 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 2 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Ms25 | 39 | 60 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Ms26 | 29 | 21 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 |
Ms27 | 39 | 53 | 0 | 0 | 4 | 2 | 1 | 1 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms28 | 53 | 46 | 1 | 1 | 5 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Ms29 | 40 | 27 | 1 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms30 | 37 | 66 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Ms31 | 39 | 56 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Ms32 | 47 | 43 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Ms33 | 46 | 28 | 0 | 0 | 2 | 3 | 2 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Ms34 | 42 | 29 | 1 | 1 | 4 | 2 | 4 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms35 | 42 | 61 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Ms36 | 33 | 64 | 0 | 1 | 3 | 1 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Ms37 | 44 | 67 | 0 | 1 | 5 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms38 | 46 | 31 | 0 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Ms39 | 42 | 26 | 1 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 1 | 1 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Ms40 | 29 | 43 | 1 | 1 | 7 | 2 | 2 | 1 | 1 | 0 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Ms41 | 40 | 59 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Ms42 | 44 | 61 | 0 | 1 | 4 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms43 | 44 | 65 | 0 | 1 | 4 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Ms44 | 48 | 23 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
图5 不同生长发育阶段紫花苜蓿‘中苜1号’不同组织中MsCCoAOMT基因相对表达量A1:幼苗期叶;A2:幼苗期茎;A3:营养期叶;A4:营养期茎;A5:花期花;A6:花期茎;A7:花期叶。A1: Leaves of seedling stage; A2: Stems of seedling stage; A3: Leaves of vegetative stage; A4: Stems of vegetative stage; A5: Florets of florescence; A6: Stems of florescence; A7: Leaves of florescence.
Fig.5 Relative expression of MsCCoAOMT gene in different tissues of M. sativa ‘Zhongmu No.1’ at different growth and development stages
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