基于Meta分析菌根菌对植物叶片生理的影响

doi:10.11686/cyxb2023189

摘要/Abstract

摘要：

叶片作为植物的重要器官，其养分、光合色素、可溶性糖、可溶性蛋白等含量，以及各种酶活性都是表征其生理的重要参数；而光合作用、蒸腾作用则是叶片重要的生理活动指标。菌根菌是一类与绝大多数植物形成共生的土壤微生物，能够影响叶片生理。本研究选择分布最广泛的丛枝菌根菌（arbuscular mycorrhizal fungi， AMF），针对以往大多数研究只关注单一植物接种AMF探究其生理功能的问题，采用Meta分析（Meta-analysis）的定量研究方法，对152篇相关文献整理建库，评估了AMF对植物叶片生理的影响，探究了不同分类水平上AMF的效应。结果表明：AMF显著提高了叶片氮、磷、钾、钙、镁和锌含量，提高了12.6%~26.3%；增加了叶绿素、叶绿素a和叶绿素b含量，分别增加了16.3%、12.1%和11.1%；对可溶性糖和可溶性蛋白则分别提升了34.8%和18.4%；同时，AMF提高了叶片各种酶活性，分别为过氧化物酶31.3%、超氧化物歧化酶17.8%、过氧化氢酶23.3%、多酚氧化酶59.1%、苯丙氨酸解氨酶65.3%；AMF提高了叶片的净光合速率（27.7%）和蒸腾速率（31.1%）。表明AMF因分类（科、属、种）不同，对叶片生理的影响也存在差异。本研究系统评价了AMF对叶片生理的影响，丰富了AMF的功能多样性理论，为筛选改善叶片生理的高效AMF提供了切实的依据。

关键词: 菌根菌, 丛枝菌根真菌, 叶片生理, 接菌效应, Meta分析

Abstract:

Leaves are important plant organs， and their contents of nutrients， photosynthetic pigments， soluble sugars， and soluble proteins， as well as the activities of various enzymes， are important parameters that characterize their physiology. Photosynthesis and transpiration are important indicators of physiological activity in leaves. Mycorrhizal fungi are soil microorganisms that form symbioses with the vast majority of plants， and they can affect leaf physiology. For this study， we selected the most widely distributed arbuscular mycorrhizal fungi （AMF） and evaluated their effects on plant leaf physiology by conducting a Meta-analysis. Most previous studies have focused on investigating the physiological functions of a single plant inoculated with one AMF. To address this issue， we used a quantitative research method and a Meta-analysis to build and analyze a database of 152 relevant papers. These analyses allowed us to evaluate the impact of AMF on plant leaf physiology， and explore the effects of AMF at different classification levels. According to the results reported in the literature， AMF significantly increased the contents of nitrogen， phosphorus， potassium， calcium， magnesium， and zinc in leaves by 12.6% to 26.3%； Increased contents of chlorophyll， chlorophyll a， and chlorophyll b by 16.3%， 12.1%， and 11.1%， respectively； And increased the contents of soluble sugars and soluble proteins by 34.8% and 18.4%， respectively. Various AMF also increased the activities of enzymes in leaves， including peroxidase （by 31.3%）， superoxide dismutase （by 17.8%）， catalase （by 23.3%）， polyphenol oxidase （by 59.1%）， and phenylalanine ammonia lyase （by 65.3%）. As a result， AMF increased the net photosynthetic rate and transpiration rate of leaves by 27.7% and 31.1%， respectively. The results of this Meta-analysis show that different taxa （family， genus， species） of AMF have different effects on leaf physiology. These findings represent a systematic evaluation of the impacts of AMF on leaf physiology， highlight the functional diversity of AMF， and provide a practical basis for screening efficient AMF to improve leaf physiology.

Key words: mycorrhiza fungi, arbuscular mycorrhizal fungi, leaf physiology, inoculation effect, Meta analysis

马路平, 石兆勇, 韦文敬, 杨爽. 基于Meta分析菌根菌对植物叶片生理的影响[J]. 草业学报, 2024, 33(4): 99-109.

Lu-ping MA, Zhao-yong SHI, Wen-jing WEI, Shuang YANG. Meta-analysis of the effects of mycorrhizal fungi on plant leaf physiology[J]. Acta Prataculturae Sinica, 2024, 33(4): 99-109.

图/表 12

图1 AMF对植物叶片养分含量的影响Ⅰ： 95%置信区间95% confidence interval；下同The same below.

Fig.1 The effect of AMF on nutrient content of plant leaves

图2 不同分类的AMF对植物叶片养分含量的影响Gl：球囊霉科Glomeraceae； Ac：无柄孢子科Acaulosporaceae； Gi：巨孢囊霉科Gigasporaceae； Cl：近明球囊霉科Claroideoglomeraceae； Fu：管柄囊霉属Funneliformis； Glo：球囊霉属Glomus； Rh：根内球囊霉属Rhizophagus； Cla：近明球囊霉属Claroideoglomus；Fm：摩西管柄囊霉Funneliformis mosseae； Gv：地表球囊霉Glomus versiforme； Ri：根内根孢囊霉Rhizophagus intraradices； Ce：幼套近明球囊霉Claroideoglomus etunicatum；下同The same below.

Fig.2 Effect of different classifications of AMF on the nutrient content of plant leaves

图3 AMF对植物叶片光合、蒸腾作用和气孔生理特性的影响

Fig.3 Effect of AMF on photosynthetic， transpiration， and stomatal physiological characteristics of plant leaves

图4 不同分类的AMF对叶片光合作用、蒸腾作用和气孔生理特性的影响

Fig.4 Effects of different classifications of AMF on leaf photosynthesis， transpiration， and stomatal physiological characteristics

图5 AMF对叶片光合色素的影响

Fig.5 Effect of AMF on photosynthetic pigments in leaves

图6 不同分类的AMF对叶片光合色素含量的影响

Fig.6 Effect of AMF of different classifications on photosynthetic pigment content in leaves

图7 AMF对叶片可溶性糖和可溶性蛋白含量的影响

Fig.7 Effect of AMF on soluble sugar and soluble protein content in leaves

图8 不同分类的AMF对叶片可溶性糖和可溶性蛋白含量的影响

Fig.8 Effects of different classifications of AMF on soluble sugar and soluble protein content in leaves

图9 AMF对叶片酶活性及丙二醛含量的影响

Fig.9 Effect of AMF on enzyme activity and malondialdehyde content in leaves

图10 不同分类的AMF对叶片酶活性及丙二醛含量的影响Gig：巨孢囊霉属Gigaspora.

Fig.10 The effect of different classified AMF on the enzyme activity and malondialdehyde content of leaves

图11 丛枝菌根真菌对植物吸收养分元素的影响①： AM真菌扩大根系吸收面积AMF expands root absorption area； ②： AM真菌促进土壤有机质矿化和矿物质溶解AMF promotes soil organic matter mineralization and mineral dissolution； ③：宿主细胞内的丛枝菌丝Arbuscular hyphae within host cells； ④， ⑤： AM真菌转运调节营养元素在宿主体内的分布AMF transport regulates the distribution of nutrients in the host body.

Fig.11 Effects of arbuscular mycorrhiza fungi on nutrient uptake by plants

图12 丛枝菌根真菌对植物叶片生理影响的过程图中数字表示的是效应值Numbers indicate the effect size； ↑：正效应Positive effect； ↓：负效应Negative effect.

Fig.12 Process mechanism of the physiological effects of AMF on plant leaves

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