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草业学报 ›› 2023, Vol. 32 ›› Issue (12): 126-138.DOI: 10.11686/cyxb2023129

• 研究论文 • 上一篇    

丛枝菌根真菌与褪黑素对多年生黑麦草耐热性的影响

卫宏健(), 贺文员, 王越, 唐明, 陈辉()   

  1. 华南农业大学林学与风景园林学院,岭南现代农业科学与技术广东省实验室,广东 广州 510642
  • 收稿日期:2023-04-24 修回日期:2023-06-08 出版日期:2023-12-20 发布日期:2023-10-18
  • 通讯作者: 陈辉
  • 作者简介:Corresponding author. E-mail: chenhui@scau.edu.cn
    卫宏健(1994-),男,广东广州人,在读博士。E-mail: weihongjian@stu.scau.edu.cn
  • 基金资助:
    国家自然科学基金(32071639);岭南现代农业科学与技术广东省实验室科研项目(NZ2021025)

The effects of arbuscular mycorrhizal fungi and melatonin on the heat tolerance of perennial ryegrass

Hong-jian WEI(), Wen-yuan HE, Yue WANG, Ming TANG, Hui CHEN()   

  1. College of Forestry and Landscape Architecture,Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou 510642,China
  • Received:2023-04-24 Revised:2023-06-08 Online:2023-12-20 Published:2023-10-18
  • Contact: Hui CHEN

摘要:

高温胁迫是限制冷季型草生长发育的主要因素。为探究单独接种丛枝菌根真菌(AMF)和外源褪黑素以及联合应用对多年生黑麦草生长和耐热性的影响,采用盆栽试验测试分析高温胁迫下丛枝菌根真菌和外源褪黑素处理对多年生黑麦草的生长,内源褪黑素含量及其合成基因的表达,抗氧化能力和渗透调节物质含量的影响。结果表明,高温胁迫明显抑制多年生黑麦草的生长,而外源褪黑素处理提高了AMF在多年生黑麦草根系中的定殖率。接种AMF和/或褪黑素处理均能促进高温胁迫下多年生黑麦草的生长,增加多年生黑麦草根系内源褪黑素含量和上调褪黑素合成基因的表达,降低相对电导率(EL)、丙二醛(MDA)含量和多酚氧化酶(PPO)活性,同时提高根系抗氧化酶(SOD、POD、CAT和APX)和苯丙氨酸解氨酶(PAL)活性,以及类黄酮、脯氨酸、总酚、可溶性糖和甜菜碱的含量。其中接种AMF和褪黑素联合应用对提高多年生黑麦草耐热性效果最佳。总的来说,AMF接种和褪黑素处理可上调多年生黑麦草根系褪黑素合成基因表达,提高内源褪黑素含量,增强抗氧化酶活性,降低氧化损伤,同时提高渗透调节能力以增强多年生黑麦草的耐热性。

关键词: 丛枝菌根真菌, 褪黑素, 多年生黑麦草, 高温胁迫, 渗透调节

Abstract:

High temperature stress is a major factor limiting the growth and development of cool-season grasses. To investigate the effects of individual inoculation with arbuscular mycorrhizal fungi (AMF), exogenous melatonin, and their combined application on the growth and heat tolerance of perennial ryegrass (Lolium perenne), a pot experiment was conducted. The current research aimed to test and analyze the effects of AMF and exogenous melatonin treatments under high temperature stress on the growth of perennial ryegrass, endogenous melatonin levels and expression of its synthesis genes, antioxidant capacity, and content of osmotic adjustment substances. The results showed that high temperature stress significantly inhibited the growth of perennial ryegrass, while exogenous melatonin treatment increased the colonization rate of AMF in the roots of perennial ryegrass. Inoculation with AMF and/or melatonin treatments promoted the growth of perennial ryegrass under high temperature stress, increased the endogenous melatonin content in the roots of perennial ryegrass, upregulated the expression of melatonin synthesis genes, reduced relative electrical conductivity (EL), malondialdehyde (MDA) content, and polyphenol oxidase (PPO) activity, and enhanced the activities of root antioxidant enzymes (SOD, POD, CAT, and APX) and phenylalanine ammonia-lyase (PAL), as well as the content of flavonoids, proline, total phenols, soluble sugars, and betaine. Notably, the combined application of AMF and melatonin showed a synergistic effect in improving the heat tolerance of perennial ryegrass. In summary, inoculation with AMF and melatonin treatment upregulated the expression of melatonin synthesis genes in the roots of perennial ryegrass, increased the endogenous melatonin content, enhanced antioxidant enzyme activities, reduced oxidative damage, and improved osmotic adjustment capacity, thereby enhancing the heat tolerance of perennial ryegrass.

Key words: arbuscular mycorrhizal fungi, melatonin, perennial ryegrass, high temperature stress, osmotic adjustment