草业学报 ›› 2024, Vol. 33 ›› Issue (6): 165-174.DOI: 10.11686/cyxb2023271
• 研究论文 • 上一篇
张昭1,2,3(), 伏莹莹4, 孙浩文4, 孙逢雪4, 闫慧芳1,2,3()
收稿日期:
2023-08-05
修回日期:
2023-09-04
出版日期:
2024-06-20
发布日期:
2024-03-20
通讯作者:
闫慧芳
作者简介:
E-mail: yanhuifang@qau.edu.cn基金资助:
Zhao ZHANG1,2,3(), Ying-ying FU4, Hao-wen SUN4, Feng-xue SUN4, Hui-fang YAN1,2,3()
Received:
2023-08-05
Revised:
2023-09-04
Online:
2024-06-20
Published:
2024-03-20
Contact:
Hui-fang YAN
摘要:
为探究不同品种燕麦种子活力与耐贮藏性差异,筛选具有高活力且耐贮藏的优异种质,本研究以8个品种燕麦种子为试验材料,采用45 ℃、100%相对湿度人工老化法模拟种子贮藏,通过测定老化0 d(即未老化,对照CK)、老化4 d两种条件下11个种子萌发与幼苗生长特性相关指标变化,运用耐老化系数、综合耐老化系数、主成分分析、隶属函数、综合评价值(D值)和系统聚类分析方法对供试种子耐贮藏性进行综合评价,以期为燕麦高活力强耐贮藏性种质筛选与新品种培育提供理论参考。结果表明:人工老化条件下,除平均发芽时间呈延长趋势外,发芽势、发芽率、发芽指数、种子活力指数、成苗率和幼苗活力指数在所有供试燕麦品种中均呈下降趋势。通过主成分分析,可将11个单项测定指标转换为3个相互独立的综合指标。根据耐贮藏性综合评价D值和系统聚类分析,将8个品种燕麦种子耐贮藏性划分为4个类型,即强耐贮藏性、中耐贮藏性、弱耐贮藏性和极弱耐贮藏性,并筛选出强耐贮藏性品种为“贝勒Ⅱ”、极弱耐贮藏性品种为“海威”,为燕麦高活力强耐贮藏性种质筛选、关键候选基因挖掘及新种质创制与利用提供了理论依据。
张昭, 伏莹莹, 孙浩文, 孙逢雪, 闫慧芳. 不同品种燕麦种子活力鉴定与耐贮藏性评价[J]. 草业学报, 2024, 33(6): 165-174.
Zhao ZHANG, Ying-ying FU, Hao-wen SUN, Feng-xue SUN, Hui-fang YAN. Identification of seed vigor and evaluation of seed storability in different varieties of oat[J]. Acta Prataculturae Sinica, 2024, 33(6): 165-174.
编号 No. | 品种 Varieties | 原产地 Place of origin | 初始发芽率Initial germination percentage (%) |
---|---|---|---|
1 | 海威Haywire | 加拿大Canada | 98 |
2 | 牧乐思Molasses | 加拿大Canada | 100 |
3 | 领袖Souris | 加拿大Canada | 99 |
4 | 太阳神Titan | 美国USA | 92 |
5 | 贝勒Baler | 加拿大Canada | 99 |
6 | 贝勒Ⅱ Baler Ⅱ | 加拿大Canada | 99 |
7 | 爱沃Everleaf | 美国USA | 94 |
8 | 三星Three stars | 加拿大Canada | 94 |
表1 8个供试燕麦品种基本信息
Table 1 Basic information for the eight tested oat varieties
编号 No. | 品种 Varieties | 原产地 Place of origin | 初始发芽率Initial germination percentage (%) |
---|---|---|---|
1 | 海威Haywire | 加拿大Canada | 98 |
2 | 牧乐思Molasses | 加拿大Canada | 100 |
3 | 领袖Souris | 加拿大Canada | 99 |
4 | 太阳神Titan | 美国USA | 92 |
5 | 贝勒Baler | 加拿大Canada | 99 |
6 | 贝勒Ⅱ Baler Ⅱ | 加拿大Canada | 99 |
7 | 爱沃Everleaf | 美国USA | 94 |
8 | 三星Three stars | 加拿大Canada | 94 |
品种 Varieties | 耐老化系数与综合耐老化系数Aging tolerance coefficient and comprehensive aging tolerance coefficient | 排序 Order | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ATC1 | ATC2 | ATC3 | ATC4 | ATC5 | ATC6 | ATC7 | ATC8 | ATC9 | ATC10 | ATC11 | CATC | ||
贝勒Ⅱ Baler Ⅱ | 0.878 | 0.913 | 0.391 | 2.088 | 0.417 | 0.845 | 0.967 | 0.893 | 1.217 | 1.269 | 0.914 | 0.981 | 1 |
太阳神Titan | 0.902 | 0.914 | 0.699 | 1.217 | 0.839 | 0.912 | 0.879 | 1.108 | 0.987 | 1.305 | 0.854 | 0.965 | 2 |
爱沃Everleaf | 0.515 | 0.818 | 0.346 | 2.066 | 0.407 | 0.759 | 1.091 | 1.151 | 1.080 | 1.190 | 0.824 | 0.932 | 3 |
贝勒Baler | 0.324 | 0.798 | 0.260 | 2.889 | 0.266 | 0.739 | 0.923 | 1.083 | 0.946 | 0.977 | 0.690 | 0.900 | 4 |
领袖Souris | 0.266 | 0.615 | 0.304 | 2.079 | 0.308 | 0.487 | 0.966 | 1.008 | 0.905 | 1.025 | 0.458 | 0.766 | 5 |
三星Three stars | 0.515 | 0.730 | 0.481 | 1.554 | 0.405 | 0.669 | 0.901 | 0.939 | 0.860 | 0.757 | 0.589 | 0.764 | 6 |
牧乐思Molasses | 0.218 | 0.313 | 0.172 | 1.988 | 0.186 | 0.237 | 0.917 | 1.010 | 0.938 | 1.168 | 0.219 | 0.670 | 7 |
海威Haywire | 0.298 | 0.531 | 0.266 | 1.979 | 0.202 | 0.436 | 0.801 | 0.773 | 0.904 | 0.741 | 0.370 | 0.664 | 8 |
表2 人工老化条件下燕麦种子萌发与幼苗生长各单项测定指标的耐老化系数
Table 2 Aging tolerance coefficient of each individual indicator for seed germination and seedling growth of oat under artificial aging condition
品种 Varieties | 耐老化系数与综合耐老化系数Aging tolerance coefficient and comprehensive aging tolerance coefficient | 排序 Order | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ATC1 | ATC2 | ATC3 | ATC4 | ATC5 | ATC6 | ATC7 | ATC8 | ATC9 | ATC10 | ATC11 | CATC | ||
贝勒Ⅱ Baler Ⅱ | 0.878 | 0.913 | 0.391 | 2.088 | 0.417 | 0.845 | 0.967 | 0.893 | 1.217 | 1.269 | 0.914 | 0.981 | 1 |
太阳神Titan | 0.902 | 0.914 | 0.699 | 1.217 | 0.839 | 0.912 | 0.879 | 1.108 | 0.987 | 1.305 | 0.854 | 0.965 | 2 |
爱沃Everleaf | 0.515 | 0.818 | 0.346 | 2.066 | 0.407 | 0.759 | 1.091 | 1.151 | 1.080 | 1.190 | 0.824 | 0.932 | 3 |
贝勒Baler | 0.324 | 0.798 | 0.260 | 2.889 | 0.266 | 0.739 | 0.923 | 1.083 | 0.946 | 0.977 | 0.690 | 0.900 | 4 |
领袖Souris | 0.266 | 0.615 | 0.304 | 2.079 | 0.308 | 0.487 | 0.966 | 1.008 | 0.905 | 1.025 | 0.458 | 0.766 | 5 |
三星Three stars | 0.515 | 0.730 | 0.481 | 1.554 | 0.405 | 0.669 | 0.901 | 0.939 | 0.860 | 0.757 | 0.589 | 0.764 | 6 |
牧乐思Molasses | 0.218 | 0.313 | 0.172 | 1.988 | 0.186 | 0.237 | 0.917 | 1.010 | 0.938 | 1.168 | 0.219 | 0.670 | 7 |
海威Haywire | 0.298 | 0.531 | 0.266 | 1.979 | 0.202 | 0.436 | 0.801 | 0.773 | 0.904 | 0.741 | 0.370 | 0.664 | 8 |
指标 Indicator | 发芽势 GE | 发芽率 GP | 发芽指数 GI | 平均发芽时间 MGT | 种子活力指数 VI | 成苗率 SP | 苗长 SL | 苗重 SW | 根长 RL | 根重 RW |
---|---|---|---|---|---|---|---|---|---|---|
发芽率GP | 0.76** | |||||||||
发芽指数GI | 0.95** | 0.90** | ||||||||
平均发芽时间MGT | -0.74** | -0.21 | -0.60** | |||||||
种子活力指数VI | 0.92** | 0.87** | 0.96** | -0.55** | ||||||
成苗率SP | 0.78** | 0.97** | 0.90** | -0.25 | 0.88** | |||||
苗长SL | 0.15 | 0.22 | 0.14 | 0.01 | 0.23 | 0.16 | ||||
苗重SW | -0.19 | -0.07 | -0.14 | 0.23 | 0.11 | -0.02 | 0.29 | |||
根长RL | 0.52** | 0.39* | 0.47** | -0.37* | 0.54** | 0.36* | 0.81** | 0.12 | ||
根重RW | 0.27 | 0.10 | 0.19 | -0.17 | 0.43* | 0.15 | 0.43* | 0.63** | 0.53** | |
幼苗活力指数SVI | 0.79** | 0.95** | 0.89** | -0.28 | 0.89** | 0.96** | 0.41* | 0.00 | 0.59** | 0.24 |
表3 人工老化条件下燕麦种子萌发与幼苗生长各单项指标的相关系数矩阵
Table 3 Correlation coefficient matrix of each individual indicator for seed germination and seedling growth of oat under artificial aging condition
指标 Indicator | 发芽势 GE | 发芽率 GP | 发芽指数 GI | 平均发芽时间 MGT | 种子活力指数 VI | 成苗率 SP | 苗长 SL | 苗重 SW | 根长 RL | 根重 RW |
---|---|---|---|---|---|---|---|---|---|---|
发芽率GP | 0.76** | |||||||||
发芽指数GI | 0.95** | 0.90** | ||||||||
平均发芽时间MGT | -0.74** | -0.21 | -0.60** | |||||||
种子活力指数VI | 0.92** | 0.87** | 0.96** | -0.55** | ||||||
成苗率SP | 0.78** | 0.97** | 0.90** | -0.25 | 0.88** | |||||
苗长SL | 0.15 | 0.22 | 0.14 | 0.01 | 0.23 | 0.16 | ||||
苗重SW | -0.19 | -0.07 | -0.14 | 0.23 | 0.11 | -0.02 | 0.29 | |||
根长RL | 0.52** | 0.39* | 0.47** | -0.37* | 0.54** | 0.36* | 0.81** | 0.12 | ||
根重RW | 0.27 | 0.10 | 0.19 | -0.17 | 0.43* | 0.15 | 0.43* | 0.63** | 0.53** | |
幼苗活力指数SVI | 0.79** | 0.95** | 0.89** | -0.28 | 0.89** | 0.96** | 0.41* | 0.00 | 0.59** | 0.24 |
指标 Indicator | 综合指标Comprehensive indicator | ||
---|---|---|---|
CI1 | CI2 | CI3 | |
GE GP | 0.935 0.905 | -0.199 -0.199 | -0.241 0.353 |
GI | 0.966 | -0.242 | -0.027 |
MGT | -0.577 | 0.185 | 0.729 |
VI | 0.977 | -0.012 | 0.038 |
SP | 0.898 | -0.200 | 0.391 |
SL | 0.363 | 0.730 | -0.080 |
SW | -0.003 | 0.773 | 0.369 |
RL | 0.674 | 0.551 | -0.347 |
RW | 0.374 | 0.770 | -0.089 |
SVI | 0.948 | -0.025 | 0.275 |
特征值Eigen values | 6.344 | 2.239 | 1.215 |
贡献率Contributive rate (%) | 57.674 | 20.357 | 11.042 |
累计贡献率Cumulative contributive rate (%) | 57.674 | 78.031 | 89.073 |
表4 基于主成分分析的各综合指标的系数及贡献率
Table 4 Coefficient and contributive rate of each comprehensive indicator based on principal component analysis
指标 Indicator | 综合指标Comprehensive indicator | ||
---|---|---|---|
CI1 | CI2 | CI3 | |
GE GP | 0.935 0.905 | -0.199 -0.199 | -0.241 0.353 |
GI | 0.966 | -0.242 | -0.027 |
MGT | -0.577 | 0.185 | 0.729 |
VI | 0.977 | -0.012 | 0.038 |
SP | 0.898 | -0.200 | 0.391 |
SL | 0.363 | 0.730 | -0.080 |
SW | -0.003 | 0.773 | 0.369 |
RL | 0.674 | 0.551 | -0.347 |
RW | 0.374 | 0.770 | -0.089 |
SVI | 0.948 | -0.025 | 0.275 |
特征值Eigen values | 6.344 | 2.239 | 1.215 |
贡献率Contributive rate (%) | 57.674 | 20.357 | 11.042 |
累计贡献率Cumulative contributive rate (%) | 57.674 | 78.031 | 89.073 |
品种 Varieties | F (X1) | F (X2) | F (X3) | μ (X1) | μ (X2) | μ (X3) | D值 D value | 综合评价及排序 Comprehensive evaluation and order |
---|---|---|---|---|---|---|---|---|
贝勒Ⅱ Baler Ⅱ | 1.551 | 0.262 | -0.906 | 1.000 | 0.552 | 0.082 | 0.784 | 1 |
太阳神Titan | 1.150 | -0.758 | -0.350 | 0.852 | 0.213 | 0.253 | 0.632 | 2 |
爱沃Everleaf | 0.437 | 0.701 | 0.283 | 0.589 | 0.699 | 0.447 | 0.596 | 3 |
贝勒Baler | 0.030 | 0.749 | 2.085 | 0.439 | 0.714 | 1.000 | 0.571 | 4 |
三星Three stars | -0.242 | -0.805 | 0.405 | 0.338 | 0.197 | 0.484 | 0.324 | 5 |
领袖Souris | -0.641 | -0.359 | -0.054 | 0.191 | 0.346 | 0.344 | 0.245 | 6 |
牧乐思Molasses | -1.158 | 1.606 | -1.174 | 0.000 | 1.000 | 0.000 | 0.229 | 7 |
海威Haywire | -1.126 | -1.396 | -0.289 | 0.012 | 0.000 | 0.272 | 0.041 | 8 |
权重Index weight | 0.647 | 0.229 | 0.124 |
表5 不同品种燕麦种子耐贮藏性的综合指标值F(Xi )、隶属函数值μ(Xi )、D值及综合评价
Table 5 Comprehensive indicator F(Xi ), membership function value μ(Xi ), D value and comprehensive evaluation of seed storability in different varieties of oat
品种 Varieties | F (X1) | F (X2) | F (X3) | μ (X1) | μ (X2) | μ (X3) | D值 D value | 综合评价及排序 Comprehensive evaluation and order |
---|---|---|---|---|---|---|---|---|
贝勒Ⅱ Baler Ⅱ | 1.551 | 0.262 | -0.906 | 1.000 | 0.552 | 0.082 | 0.784 | 1 |
太阳神Titan | 1.150 | -0.758 | -0.350 | 0.852 | 0.213 | 0.253 | 0.632 | 2 |
爱沃Everleaf | 0.437 | 0.701 | 0.283 | 0.589 | 0.699 | 0.447 | 0.596 | 3 |
贝勒Baler | 0.030 | 0.749 | 2.085 | 0.439 | 0.714 | 1.000 | 0.571 | 4 |
三星Three stars | -0.242 | -0.805 | 0.405 | 0.338 | 0.197 | 0.484 | 0.324 | 5 |
领袖Souris | -0.641 | -0.359 | -0.054 | 0.191 | 0.346 | 0.344 | 0.245 | 6 |
牧乐思Molasses | -1.158 | 1.606 | -1.174 | 0.000 | 1.000 | 0.000 | 0.229 | 7 |
海威Haywire | -1.126 | -1.396 | -0.289 | 0.012 | 0.000 | 0.272 | 0.041 | 8 |
权重Index weight | 0.647 | 0.229 | 0.124 |
图1 基于耐贮藏性综合评价值(D值)的系统聚类分析类型Ⅰ,强耐贮藏性;类型Ⅱ,中耐贮藏性;类型Ⅲ,弱耐贮藏性;类型Ⅳ,极弱耐贮藏性。Type Ⅰ, strong storability; Type Ⅱ, medium storability; Type Ⅲ, weak storability; Type Ⅳ, extremely weak storability.
Fig. 1 Cluster analysis based on comprehensive evaluation value (D value) of storability
相对耐贮藏性等级Relative storability level | D值D value |
---|---|
强耐贮藏性Strong storability | D≥0.784 |
中耐贮藏性Medium storability | 0.571≤D<0.784 |
弱耐贮藏性Weak storability | 0.229≤D<0.571 |
极弱耐贮藏性Extremely weak storability | D<0.229 |
表6 不同品种供试燕麦种子耐贮藏性等级划分
Table 6 Classification of seed storability of different varieties of tested oat
相对耐贮藏性等级Relative storability level | D值D value |
---|---|
强耐贮藏性Strong storability | D≥0.784 |
中耐贮藏性Medium storability | 0.571≤D<0.784 |
弱耐贮藏性Weak storability | 0.229≤D<0.571 |
极弱耐贮藏性Extremely weak storability | D<0.229 |
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