草业学报 ›› 2023, Vol. 32 ›› Issue (6): 85-99.DOI: 10.11686/cyxb2022322
张适阳1(), 刘凤民2(), 崔均涛4, 何磊1, 冯月燕3, 张伟丽1,3()
收稿日期:
2022-08-10
修回日期:
2022-12-05
出版日期:
2023-06-20
发布日期:
2023-04-21
通讯作者:
张伟丽
作者简介:
Corresponding author. E-mail: zhangweili7218@163.com基金资助:
Shi-yang ZHANG1(), Feng-min LIU2(), Jun-tao CUI4, Lei HE1, Yue-yan FENG3, Wei-li ZHANG1,3()
Received:
2022-08-10
Revised:
2022-12-05
Online:
2023-06-20
Published:
2023-04-21
Contact:
Wei-li ZHANG
摘要:
为研究不同浓度脱落酸、壳聚糖和水杨酸喷施对低温胁迫下柱花草叶片生理指标和叶绿素荧光特性的影响,以播种50 d的幼苗为试验材料,叶面喷施法处理后,于光照培养箱中连续进行7 d低温(昼夜温8 ℃/5 ℃),2 d常温(昼夜温28 ℃/25 ℃)处理。结果表明,不同浓度外源物质喷施使低温下柱花草叶片光合色素含量、抗氧化酶活性、可溶性蛋白含量显著增加;寒害指数、相对电导率、丙二醛含量显著降低,继续2 d常温处理后,各项指标有所回升。经300 mg·L-1壳聚糖、0.4 mmol·L-1水杨酸和10 mg·L-1脱落酸处理的植株,叶绿素荧光参数的最大光化学效率(Fv/Fm)、电子传递量子产额(φE0)、性能参数(PIABS)分别显著升高了80.88%~122.57%、119.87%~170.53%和271.77%~580.49%;用于热耗散量子比率(φD0)显著降低了26.39%~37.10%。经隶属函数法综合评价,壳聚糖、脱落酸、水杨酸提高柱花草低温耐受性的最佳诱导浓度分别为:300 mg·L-1,10 mg·L-1,0.4 mmol·L-1。在3种外源物质中,300 mg·L-1壳聚糖处理可显著提高叶绿素含量和光系统Ⅱ(PSⅡ)的活性,减少热耗散,对缓解低温胁迫对柱花草的伤害效果最为显著。
张适阳, 刘凤民, 崔均涛, 何磊, 冯月燕, 张伟丽. 三种外源物质对低温胁迫下柱花草生理与荧光特性的影响[J]. 草业学报, 2023, 32(6): 85-99.
Shi-yang ZHANG, Feng-min LIU, Jun-tao CUI, Lei HE, Yue-yan FENG, Wei-li ZHANG. Effects of three exogenous substances on the physiological and fluorescence characteristics of Stylosanthes guianensis under low-temperature stress[J]. Acta Prataculturae Sinica, 2023, 32(6): 85-99.
外源物质 Exogenous substances | 浓度 Concentration | 寒害指数 Chilling injury index |
---|---|---|
壳聚糖 Chitosan (mg·L-1) | 0 | 0.66±0.01a |
300 | 0.46±0.01c | |
400 | 0.48±0.02bc | |
500 | 0.49±0.01b | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 0.59±0.01a |
0.2 | 0.53±0.02b | |
0.4 | 0.50±0.02c | |
0.6 | 0.52±0.02bc | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 0.66±0.01a |
5 | 0.49±0.01c | |
10 | 0.48±0.01c | |
15 | 0.52±0.03b |
表1 低温胁迫下外源物质施用对柱花草抗寒指数的影响
Table 1 Effects of exogenous substance application on chilling injury index of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | 寒害指数 Chilling injury index |
---|---|---|
壳聚糖 Chitosan (mg·L-1) | 0 | 0.66±0.01a |
300 | 0.46±0.01c | |
400 | 0.48±0.02bc | |
500 | 0.49±0.01b | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 0.59±0.01a |
0.2 | 0.53±0.02b | |
0.4 | 0.50±0.02c | |
0.6 | 0.52±0.02bc | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 0.66±0.01a |
5 | 0.49±0.01c | |
10 | 0.48±0.01c | |
15 | 0.52±0.03b |
外源物质 Exogenous substances | 浓度 Concentration | 叶绿素含量Chlorophyll content (mg·g-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 42.97±2.28a | 43.15±0.69a | 35.23±0.28c | 33.48±1.40b | 29.93±0.78c | 28.55±0.64b |
300 | 43.99±0.79a | 43.84±0.67a | 42.50±0.37a | 39.53±1.27a | 37.04±0.89a | 34.78±0.91a | |
400 | 44.81±0.28a | 43.44±0.53a | 41.82±0.50a | 37.11±1.89a | 35.35±0.80a | 35.72±0.13a | |
500 | 43.80±0.41a | 43.49±0.76a | 39.55±0.34b | 37.32±2.09a | 33.86±0.24b | 34.57±1.38a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 44.99±0.29a | 44.36±2.69a | 36.09±0.45c | 32.30±1.18d | 28.12±0.20d | 27.15±0.54d |
0.2 | 45.07±0.76a | 44.14±0.11a | 41.67±0.83b | 36.43±1.14c | 33.74±1.06c | 32.50±0.95c | |
0.4 | 46.14±1.66a | 44.71±3.27a | 44.36±0.49a | 42.16±0.75a | 37.85±0.89a | 37.07±1.14a | |
0.6 | 44.82±1.15a | 44.48±0.73a | 43.35±1.00a | 38.82±1.32b | 35.47±0.61b | 35.47±0.52b | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 44.99±0.29a | 43.69±1.85a | 36.09±0.45c | 30.96±1.17b | 26.10±0.20d | 26.12±0.62c |
5 | 46.76±1.08a | 43.64±0.63a | 38.05±0.71b | 34.68±1.84a | 29.32±0.27c | 29.46±0.51b | |
10 | 45.94±2.10a | 43.19±1.47a | 41.18±0.91a | 36.38±1.11a | 33.63±0.23a | 34.12±1.73a | |
15 | 46.48±1.29a | 43.35±0.81a | 40.27±0.79a | 35.89±0.22a | 31.38±0.35b | 32.12±1.03a |
表2 低温胁迫下外源物质施用对柱花草叶绿素含量的影响
Table 2 Effects of exogenous substances application on chlorophyll content of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | 叶绿素含量Chlorophyll content (mg·g-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 42.97±2.28a | 43.15±0.69a | 35.23±0.28c | 33.48±1.40b | 29.93±0.78c | 28.55±0.64b |
300 | 43.99±0.79a | 43.84±0.67a | 42.50±0.37a | 39.53±1.27a | 37.04±0.89a | 34.78±0.91a | |
400 | 44.81±0.28a | 43.44±0.53a | 41.82±0.50a | 37.11±1.89a | 35.35±0.80a | 35.72±0.13a | |
500 | 43.80±0.41a | 43.49±0.76a | 39.55±0.34b | 37.32±2.09a | 33.86±0.24b | 34.57±1.38a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 44.99±0.29a | 44.36±2.69a | 36.09±0.45c | 32.30±1.18d | 28.12±0.20d | 27.15±0.54d |
0.2 | 45.07±0.76a | 44.14±0.11a | 41.67±0.83b | 36.43±1.14c | 33.74±1.06c | 32.50±0.95c | |
0.4 | 46.14±1.66a | 44.71±3.27a | 44.36±0.49a | 42.16±0.75a | 37.85±0.89a | 37.07±1.14a | |
0.6 | 44.82±1.15a | 44.48±0.73a | 43.35±1.00a | 38.82±1.32b | 35.47±0.61b | 35.47±0.52b | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 44.99±0.29a | 43.69±1.85a | 36.09±0.45c | 30.96±1.17b | 26.10±0.20d | 26.12±0.62c |
5 | 46.76±1.08a | 43.64±0.63a | 38.05±0.71b | 34.68±1.84a | 29.32±0.27c | 29.46±0.51b | |
10 | 45.94±2.10a | 43.19±1.47a | 41.18±0.91a | 36.38±1.11a | 33.63±0.23a | 34.12±1.73a | |
15 | 46.48±1.29a | 43.35±0.81a | 40.27±0.79a | 35.89±0.22a | 31.38±0.35b | 32.12±1.03a |
外源物质 Exogenous substances | 浓度 Concentration | 类胡萝卜素含量Carotenoid content (mg·g-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 21.86±0.06b | 20.61±1.22c | 18.73±0.45c | 14.19±0.08d | 12.65±0.24d | 12.66±0.30d |
300 | 24.85±1.49a | 24.88±0.84a | 22.24±0.60a | 17.52±0.52a | 16.10±0.23a | 15.79±0.25a | |
400 | 24.43±0.19a | 24.19±0.43ab | 20.46±1.39b | 16.40±0.24b | 15.33±0.32b | 14.73±0.30b | |
500 | 24.66±0.95a | 23.10±0.49b | 19.24±0.49bc | 15.52±0.33c | 14.42±0.53c | 14.18±0.50c | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 20.46±0.40a | 18.91±0.43a | 17.98±0.12a | 14.50±0.52c | 13.98±0.04c | 14.51±0.26b |
0.2 | 20.87±0.56a | 19.64±0.96a | 18.21±0.27a | 15.74±0.43b | 15.03±0.14b | 15.49±0.47ab | |
0.4 | 20.33±0.81a | 19.67±0.15a | 18.88±0.93a | 17.01±0.48a | 15.63±0.44a | 15.95±0.88a | |
0.6 | 20.43±0.24a | 19.53±0.93a | 18.63±0.53a | 15.41±0.37b | 14.52±0.36bc | 14.76±0.10b | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 20.46±0.40a | 15.72±0.20b | 13.73±0.17b | 10.74±0.53c | 8.73±0.03d | 8.54±0.43d |
5 | 20.51±1.20a | 16.70±0.36a | 16.02±0.77a | 13.57±0.18b | 11.76±0.59c | 11.79±0.20c | |
10 | 20.70±0.94a | 17.10±0.67a | 15.02±0.81a | 15.13±0.31a | 14.50±0.69a | 14.45±0.65a | |
15 | 19.96±1.65a | 16.22±0.50ab | 15.21±0.29a | 14.39±0.76ab | 13.11±0.38b | 12.71±0.35b |
表3 低温胁迫下外源物质施用对柱花草类胡萝卜素含量的影响
Table 3 Effects of exogenous substances application on carotenoid content of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | 类胡萝卜素含量Carotenoid content (mg·g-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 21.86±0.06b | 20.61±1.22c | 18.73±0.45c | 14.19±0.08d | 12.65±0.24d | 12.66±0.30d |
300 | 24.85±1.49a | 24.88±0.84a | 22.24±0.60a | 17.52±0.52a | 16.10±0.23a | 15.79±0.25a | |
400 | 24.43±0.19a | 24.19±0.43ab | 20.46±1.39b | 16.40±0.24b | 15.33±0.32b | 14.73±0.30b | |
500 | 24.66±0.95a | 23.10±0.49b | 19.24±0.49bc | 15.52±0.33c | 14.42±0.53c | 14.18±0.50c | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 20.46±0.40a | 18.91±0.43a | 17.98±0.12a | 14.50±0.52c | 13.98±0.04c | 14.51±0.26b |
0.2 | 20.87±0.56a | 19.64±0.96a | 18.21±0.27a | 15.74±0.43b | 15.03±0.14b | 15.49±0.47ab | |
0.4 | 20.33±0.81a | 19.67±0.15a | 18.88±0.93a | 17.01±0.48a | 15.63±0.44a | 15.95±0.88a | |
0.6 | 20.43±0.24a | 19.53±0.93a | 18.63±0.53a | 15.41±0.37b | 14.52±0.36bc | 14.76±0.10b | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 20.46±0.40a | 15.72±0.20b | 13.73±0.17b | 10.74±0.53c | 8.73±0.03d | 8.54±0.43d |
5 | 20.51±1.20a | 16.70±0.36a | 16.02±0.77a | 13.57±0.18b | 11.76±0.59c | 11.79±0.20c | |
10 | 20.70±0.94a | 17.10±0.67a | 15.02±0.81a | 15.13±0.31a | 14.50±0.69a | 14.45±0.65a | |
15 | 19.96±1.65a | 16.22±0.50ab | 15.21±0.29a | 14.39±0.76ab | 13.11±0.38b | 12.71±0.35b |
外源物质 Exogenous substances | 浓度 Concentration | 相对电导率Relative conductivity (%) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 24.20±0.71b | 40.61±1.59b | 53.36±1.01a | 63.44±2.97a | 73.98±4.00a | 54.69±2.85a |
300 | 25.91±1.27a | 38.78±1.42c | 45.32±2.08c | 51.75±2.35c | 56.28±2.31d | 41.91±1.55b | |
400 | 25.02±1.29ab | 42.06±1.60a | 48.74±2.49b | 55.57±2.02b | 60.03±1.16c | 38.73±3.94c | |
500 | 25.07±1.84ab | 43.37±0.90a | 49.77±3.98b | 56.51±0.72b | 66.34±1.14b | 52.45±3.47a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 26.87±1.12a | 39.56±1.94a | 47.46±0.41a | 57.68±1.09a | 70.37±0.39a | 57.04±0.45a |
0.2 | 25.48±2.24a | 37.36±1.88bc | 43.26±3.53b | 48.14±1.69c | 54.90±0.62c | 46.74±3.83b | |
0.4 | 26.96±2.37a | 35.94±1.04c | 40.63±1.56c | 46.27±0.69d | 49.90±1.08d | 44.62±1.78b | |
0.6 | 27.10±2.24a | 38.64±1.06ab | 45.72±0.92ab | 51.71±1.26b | 60.48±1.25b | 47.68±2.51b | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 26.87±1.12a | 39.56±1.94a | 47.46±0.41a | 57.68±1.09a | 70.37±0.39a | 57.04±0.45a |
5 | 27.11±5.67a | 31.57±1.89c | 39.32±1.40b | 42.59±1.49c | 48.83±1.38c | 37.19±2.17c | |
10 | 26.95±1.02a | 31.14±1.29c | 35.84±1.41c | 38.87±1.82d | 42.95±1.57d | 35.21±2.46c | |
15 | 26.21±1.42a | 33.94±1.50b | 39.60±0.97b | 46.04±1.90b | 54.35±0.88b | 40.13±1.26b |
表4 低温胁迫下外源物质施用对柱花草相对电导率的影响
Table 4 Effects of exogenous substances application on relative conductivity of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | 相对电导率Relative conductivity (%) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 24.20±0.71b | 40.61±1.59b | 53.36±1.01a | 63.44±2.97a | 73.98±4.00a | 54.69±2.85a |
300 | 25.91±1.27a | 38.78±1.42c | 45.32±2.08c | 51.75±2.35c | 56.28±2.31d | 41.91±1.55b | |
400 | 25.02±1.29ab | 42.06±1.60a | 48.74±2.49b | 55.57±2.02b | 60.03±1.16c | 38.73±3.94c | |
500 | 25.07±1.84ab | 43.37±0.90a | 49.77±3.98b | 56.51±0.72b | 66.34±1.14b | 52.45±3.47a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 26.87±1.12a | 39.56±1.94a | 47.46±0.41a | 57.68±1.09a | 70.37±0.39a | 57.04±0.45a |
0.2 | 25.48±2.24a | 37.36±1.88bc | 43.26±3.53b | 48.14±1.69c | 54.90±0.62c | 46.74±3.83b | |
0.4 | 26.96±2.37a | 35.94±1.04c | 40.63±1.56c | 46.27±0.69d | 49.90±1.08d | 44.62±1.78b | |
0.6 | 27.10±2.24a | 38.64±1.06ab | 45.72±0.92ab | 51.71±1.26b | 60.48±1.25b | 47.68±2.51b | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 26.87±1.12a | 39.56±1.94a | 47.46±0.41a | 57.68±1.09a | 70.37±0.39a | 57.04±0.45a |
5 | 27.11±5.67a | 31.57±1.89c | 39.32±1.40b | 42.59±1.49c | 48.83±1.38c | 37.19±2.17c | |
10 | 26.95±1.02a | 31.14±1.29c | 35.84±1.41c | 38.87±1.82d | 42.95±1.57d | 35.21±2.46c | |
15 | 26.21±1.42a | 33.94±1.50b | 39.60±0.97b | 46.04±1.90b | 54.35±0.88b | 40.13±1.26b |
外源物质 Exogenous substances | 浓度 Concentration | MDA含量Malondiadehyde content (μmol·g-1 FW) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 2.43±0.30a | 4.25±0.12a | 5.21±0.08a | 6.18±0.11a | 6.65±0.25a | 4.37±0.12a |
300 | 2.46±0.31a | 2.72±0.22d | 3.42±0.28b | 3.94±0.12d | 4.60±0.07d | 2.93±0.31b | |
400 | 2.34±0.19a | 3.67±0.06b | 4.12±0.09b | 5.00±0.07b | 5.50±0.12b | 2.73±0.40b | |
500 | 2.30±0.32a | 3.14±0.06c | 3.97±0.16c | 4.58±0.04c | 4.99±0.02c | 2.40±0.18b | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 2.88±0.14b | 4.28±0.15a | 5.21±0.07a | 6.05±0.10a | 7.34±0.06a | 6.18±0.20a |
0.2 | 2.95±0.03b | 4.28±0.10a | 4.29±0.10c | 4.64±0.24b | 6.15±0.04c | 5.59±0.11b | |
0.4 | 3.00±0.09b | 3.71±0.19b | 3.75±0.14d | 4.22±0.18c | 5.76±0.08d | 4.14±0.21c | |
0.6 | 3.28±0.19a | 4.25±0.11a | 4.67±0.20b | 4.85±0.12b | 6.62±0.04b | 4.29±0.36c | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 2.88±0.14a | 4.28±0.14a | 5.21±0.08a | 6.05±0.10a | 7.34±0.06a | 6.18±0.20a |
5 | 2.97±0.05a | 3.92±0.08b | 4.33±0.04b | 4.81±0.02b | 5.52±0.09b | 4.20±0.16b | |
10 | 2.93±0.10a | 3.12±0.01c | 3.56±0.06d | 3.88±0.10d | 4.71±0.12d | 3.73±0.21c | |
15 | 2.92±0.13a | 3.28±0.14c | 4.06±0.06c | 4.31±0.08c | 5.13±0.21c | 4.44±0.08b |
表5 低温胁迫下外源物质施用对柱花草MDA含量的影响
Table 5 Effects of exogenous substances application on MDA content of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | MDA含量Malondiadehyde content (μmol·g-1 FW) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 2.43±0.30a | 4.25±0.12a | 5.21±0.08a | 6.18±0.11a | 6.65±0.25a | 4.37±0.12a |
300 | 2.46±0.31a | 2.72±0.22d | 3.42±0.28b | 3.94±0.12d | 4.60±0.07d | 2.93±0.31b | |
400 | 2.34±0.19a | 3.67±0.06b | 4.12±0.09b | 5.00±0.07b | 5.50±0.12b | 2.73±0.40b | |
500 | 2.30±0.32a | 3.14±0.06c | 3.97±0.16c | 4.58±0.04c | 4.99±0.02c | 2.40±0.18b | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 2.88±0.14b | 4.28±0.15a | 5.21±0.07a | 6.05±0.10a | 7.34±0.06a | 6.18±0.20a |
0.2 | 2.95±0.03b | 4.28±0.10a | 4.29±0.10c | 4.64±0.24b | 6.15±0.04c | 5.59±0.11b | |
0.4 | 3.00±0.09b | 3.71±0.19b | 3.75±0.14d | 4.22±0.18c | 5.76±0.08d | 4.14±0.21c | |
0.6 | 3.28±0.19a | 4.25±0.11a | 4.67±0.20b | 4.85±0.12b | 6.62±0.04b | 4.29±0.36c | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 2.88±0.14a | 4.28±0.14a | 5.21±0.08a | 6.05±0.10a | 7.34±0.06a | 6.18±0.20a |
5 | 2.97±0.05a | 3.92±0.08b | 4.33±0.04b | 4.81±0.02b | 5.52±0.09b | 4.20±0.16b | |
10 | 2.93±0.10a | 3.12±0.01c | 3.56±0.06d | 3.88±0.10d | 4.71±0.12d | 3.73±0.21c | |
15 | 2.92±0.13a | 3.28±0.14c | 4.06±0.06c | 4.31±0.08c | 5.13±0.21c | 4.44±0.08b |
外源物质 Exogenous substances | 浓度 Concentration | POD活性Peroxidase activity (U·g-1 FW·min-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 20.60±3.70a | 34.67±1.63b | 27.20±2.71b | 18.80±0.35c | 14.93±2.53d | 26.20±2.55b |
300 | 23.13±2.27a | 44.33±0.64a | 32.33±1.62a | 30.07±0.50a | 28.73±0.90a | 31.93±2.02a | |
400 | 24.67±3.97a | 43.47±1.86a | 31.07±1.81a | 25.67±0.31b | 22.33±0.95c | 29.47±1.10ab | |
500 | 23.93±2.39a | 45.73±1.50a | 32.00±1.20a | 29.33±2.04a | 25.53±0.50b | 28.00±1.91b | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 16.91±2.47c | 26.93±0.64c | 17.93±1.15d | 13.47±1.10d | 9.93±0.64d | 33.60±0.69b |
0.2 | 18.00±1.71bc | 41.47±1.68b | 33.07±0.50c | 24.33±0.42c | 21.67±1.01c | 43.41±1.61a | |
0.4 | 26.13±0.61a | 47.53±0.70a | 42.80±0.72a | 35.87±0.61a | 29.93±1.14a | 47.93±4.43a | |
0.6 | 21.93±3.84ab | 42.99±1.58b | 34.73±0.70b | 28.93±0.58b | 24.67±0.42b | 45.00±1.59a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 16.91±2.47b | 26.93±0.64a | 17.93±1.15c | 12.80±0.53d | 9.93±0.64d | 26.45±3.03b |
5 | 17.33±0.95b | 26.73±0.61a | 21.27±0.58b | 19.80±0.60b | 17.87±0.90b | 35.60±1.31a | |
10 | 22.27±1.29a | 27.80±0.92a | 24.40±1.60a | 22.67±1.01a | 21.20±1.22a | 38.73±1.51a | |
15 | 19.87±2.47ab | 26.60±1.11a | 19.73±1.53bc | 16.20±1.25c | 13.47±0.50c | 38.93±0.81a |
表6 低温胁迫下外源物质施用对柱花草POD活性的影响
Table 6 Effects of exogenous substances application on POD activity of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | POD活性Peroxidase activity (U·g-1 FW·min-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 20.60±3.70a | 34.67±1.63b | 27.20±2.71b | 18.80±0.35c | 14.93±2.53d | 26.20±2.55b |
300 | 23.13±2.27a | 44.33±0.64a | 32.33±1.62a | 30.07±0.50a | 28.73±0.90a | 31.93±2.02a | |
400 | 24.67±3.97a | 43.47±1.86a | 31.07±1.81a | 25.67±0.31b | 22.33±0.95c | 29.47±1.10ab | |
500 | 23.93±2.39a | 45.73±1.50a | 32.00±1.20a | 29.33±2.04a | 25.53±0.50b | 28.00±1.91b | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 16.91±2.47c | 26.93±0.64c | 17.93±1.15d | 13.47±1.10d | 9.93±0.64d | 33.60±0.69b |
0.2 | 18.00±1.71bc | 41.47±1.68b | 33.07±0.50c | 24.33±0.42c | 21.67±1.01c | 43.41±1.61a | |
0.4 | 26.13±0.61a | 47.53±0.70a | 42.80±0.72a | 35.87±0.61a | 29.93±1.14a | 47.93±4.43a | |
0.6 | 21.93±3.84ab | 42.99±1.58b | 34.73±0.70b | 28.93±0.58b | 24.67±0.42b | 45.00±1.59a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 16.91±2.47b | 26.93±0.64a | 17.93±1.15c | 12.80±0.53d | 9.93±0.64d | 26.45±3.03b |
5 | 17.33±0.95b | 26.73±0.61a | 21.27±0.58b | 19.80±0.60b | 17.87±0.90b | 35.60±1.31a | |
10 | 22.27±1.29a | 27.80±0.92a | 24.40±1.60a | 22.67±1.01a | 21.20±1.22a | 38.73±1.51a | |
15 | 19.87±2.47ab | 26.60±1.11a | 19.73±1.53bc | 16.20±1.25c | 13.47±0.50c | 38.93±0.81a |
外源物质 Exogenous substances | 浓度 Concentration | CAT活性Catalase activity (U·g-1 FW·min-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 42.83±5.01a | 44.50±0.50b | 31.50±2.29b | 27.33±1.61c | 23.50±3.28d | 35.83±1.15b |
300 | 47.00±5.41a | 67.17±5.11a | 56.83±1.04a | 52.33±4.25a | 45.50±2.29a | 52.50±1.50a | |
400 | 45.33±1.61a | 66.50±3.46a | 58.50±2.18a | 49.67±0.76a | 40.67±0.29b | 56.18±2.06a | |
500 | 47.50±4.82a | 62.50±1.00a | 55.50±2.00a | 44.17±0.76b | 35.83±3.01c | 53.83±2.52a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 42.83±1.61a | 44.50±0.50c | 41.17±0.58d | 31.67±0.76c | 23.50±0.50d | 28.00±1.32b |
0.2 | 41.50±0.50a | 46.00±0.50b | 48.33±0.76c | 37.33±0.29b | 26.67±0.76c | 32.67±1.61a | |
0.4 | 42.17±1.44a | 49.50±0.50a | 53.83±1.04a | 41.50±0.50a | 31.67±0.58a | 33.33±1.53a | |
0.6 | 43.33±3.79a | 46.00±0.87b | 51.33±1.04b | 38.33±2.08b | 28.33±0.76b | 32.17±1.61a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 41.17±2.25a | 44.50±0.50d | 39.67±1.44d | 33.17±1.89d | 22.33±1.61d | 28.17±3.06b |
5 | 42.00±1.32a | 50.50±0.50c | 56.50±1.32c | 46.33±0.29c | 37.67±1.76c | 44.67±2.08a | |
10 | 42.00±3.04a | 55.00±0.50a | 63.83±1.15a | 55.85±1.17a | 46.17±2.08a | 48.00±1.80a | |
15 | 42.33±2.31a | 53.17±1.53b | 60.33±0.76b | 50.67±1.26b | 41.17±0.76b | 44.33±0.29a |
表7 低温胁迫下外源物质施用对柱花草CAT活性的影响
Table 7 Effects of exogenous substances application on CAT activity of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | CAT活性Catalase activity (U·g-1 FW·min-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 42.83±5.01a | 44.50±0.50b | 31.50±2.29b | 27.33±1.61c | 23.50±3.28d | 35.83±1.15b |
300 | 47.00±5.41a | 67.17±5.11a | 56.83±1.04a | 52.33±4.25a | 45.50±2.29a | 52.50±1.50a | |
400 | 45.33±1.61a | 66.50±3.46a | 58.50±2.18a | 49.67±0.76a | 40.67±0.29b | 56.18±2.06a | |
500 | 47.50±4.82a | 62.50±1.00a | 55.50±2.00a | 44.17±0.76b | 35.83±3.01c | 53.83±2.52a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 42.83±1.61a | 44.50±0.50c | 41.17±0.58d | 31.67±0.76c | 23.50±0.50d | 28.00±1.32b |
0.2 | 41.50±0.50a | 46.00±0.50b | 48.33±0.76c | 37.33±0.29b | 26.67±0.76c | 32.67±1.61a | |
0.4 | 42.17±1.44a | 49.50±0.50a | 53.83±1.04a | 41.50±0.50a | 31.67±0.58a | 33.33±1.53a | |
0.6 | 43.33±3.79a | 46.00±0.87b | 51.33±1.04b | 38.33±2.08b | 28.33±0.76b | 32.17±1.61a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 41.17±2.25a | 44.50±0.50d | 39.67±1.44d | 33.17±1.89d | 22.33±1.61d | 28.17±3.06b |
5 | 42.00±1.32a | 50.50±0.50c | 56.50±1.32c | 46.33±0.29c | 37.67±1.76c | 44.67±2.08a | |
10 | 42.00±3.04a | 55.00±0.50a | 63.83±1.15a | 55.85±1.17a | 46.17±2.08a | 48.00±1.80a | |
15 | 42.33±2.31a | 53.17±1.53b | 60.33±0.76b | 50.67±1.26b | 41.17±0.76b | 44.33±0.29a |
外源物质 Exogenous substances | 浓度 Concentration | SOD活性Superoxide dismutase activity (U·g-1 FW·min-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 169.99±7.62a | 192.83±10.34b | 160.26±5.03c | 125.78±6.02c | 94.64±6.74c | 135.66±5.15b |
300 | 168.91±2.47a | 217.21±7.34a | 192.27±4.80a | 177.14±1.71a | 144.37±1.90a | 168.40±3.01a | |
400 | 176.24±16.06a | 201.31±5.78b | 175.94±1.02b | 148.31±2.81b | 124.14±5.10b | 168.09±5.61a | |
500 | 176.45±10.24a | 203.77±5.18ab | 169.30±3.95b | 132.03±6.02c | 116.09±4.78b | 165.24±3.77a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 214.66±4.26a | 218.50±6.54c | 168.80±13.03c | 125.78±6.02d | 94.64±6.74d | 175.24±6.73b |
0.2 | 217.26±21.97a | 237.48±6.76b | 192.75±8.18b | 160.71±4.80c | 131.18±2.33c | 191.13±6.30a | |
0.4 | 212.45±9.07a | 270.79±8.72a | 230.16±12.64a | 196.93±2.41a | 168.99±1.70a | 182.11±8.32ab | |
0.6 | 215.93±10.72a | 242.55±6.03b | 211.76±3.31a | 173.41±5.07b | 141.83±2.76b | 190.79±5.23a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 214.66±4.26a | 230.92±7.82b | 164.53±6.54d | 125.78±6.02d | 94.64±4.07c | 177.88±4.84ab |
5 | 211.46±7.67a | 228.52±4.31b | 179.48±8.11c | 137.51±5.07c | 108.33±6.50b | 174.85±4.04b | |
10 | 210.88±22.90a | 246.43±3.68a | 214.33±3.63a | 174.71±5.34a | 130.08±3.55a | 186.20±3.68a | |
15 | 210.88±22.90a | 232.27±8.59b | 191.91±3.00b | 160.40±0.53b | 115.42±6.06b | 181.84±7.48ab |
表8 低温胁迫下外源物质施用对柱花草SOD活性的影响
Table 8 Effects of exogenous substances application on SOD activity of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | SOD活性Superoxide dismutase activity (U·g-1 FW·min-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 169.99±7.62a | 192.83±10.34b | 160.26±5.03c | 125.78±6.02c | 94.64±6.74c | 135.66±5.15b |
300 | 168.91±2.47a | 217.21±7.34a | 192.27±4.80a | 177.14±1.71a | 144.37±1.90a | 168.40±3.01a | |
400 | 176.24±16.06a | 201.31±5.78b | 175.94±1.02b | 148.31±2.81b | 124.14±5.10b | 168.09±5.61a | |
500 | 176.45±10.24a | 203.77±5.18ab | 169.30±3.95b | 132.03±6.02c | 116.09±4.78b | 165.24±3.77a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 214.66±4.26a | 218.50±6.54c | 168.80±13.03c | 125.78±6.02d | 94.64±6.74d | 175.24±6.73b |
0.2 | 217.26±21.97a | 237.48±6.76b | 192.75±8.18b | 160.71±4.80c | 131.18±2.33c | 191.13±6.30a | |
0.4 | 212.45±9.07a | 270.79±8.72a | 230.16±12.64a | 196.93±2.41a | 168.99±1.70a | 182.11±8.32ab | |
0.6 | 215.93±10.72a | 242.55±6.03b | 211.76±3.31a | 173.41±5.07b | 141.83±2.76b | 190.79±5.23a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 214.66±4.26a | 230.92±7.82b | 164.53±6.54d | 125.78±6.02d | 94.64±4.07c | 177.88±4.84ab |
5 | 211.46±7.67a | 228.52±4.31b | 179.48±8.11c | 137.51±5.07c | 108.33±6.50b | 174.85±4.04b | |
10 | 210.88±22.90a | 246.43±3.68a | 214.33±3.63a | 174.71±5.34a | 130.08±3.55a | 186.20±3.68a | |
15 | 210.88±22.90a | 232.27±8.59b | 191.91±3.00b | 160.40±0.53b | 115.42±6.06b | 181.84±7.48ab |
外源物质 Exogenous substances | 浓度 Concentration | 可溶性蛋白含量Soluble protein content (mg·g-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 5.61±0.36a | 5.54±0.25c | 4.70±0.41c | 3.50±0.29c | 3.42±0.05d | 4.53±0.35b |
300 | 5.47±1.14a | 7.31±0.29a | 6.92±0.34a | 6.40±0.04a | 5.54±0.07a | 6.60±0.32a | |
400 | 5.27±1.20a | 7.34±0.27a | 6.65±0.23a | 6.22±0.21a | 5.24±0.19b | 6.40±0.20a | |
500 | 5.73±0.28a | 6.61±0.38b | 6.02±0.21b | 5.37±0.42b | 4.89±0.16c | 6.70±0.36a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 8.49±1.26a | 9.58±0.38c | 8.42±0.34c | 6.47±0.27d | 5.33±0.11d | 6.48±0.10b |
0.2 | 9.34±0.89a | 10.61±0.41b | 9.70±0.26b | 8.33±0.20c | 6.75±0.41c | 9.01±0.21a | |
0.4 | 9.84±0.98a | 11.33±0.28a | 10.28±0.05a | 9.42±0.13a | 8.66±0.05a | 9.07±0.33a | |
0.6 | 9.80±0.40a | 10.67±0.29b | 9.34±0.19b | 8.78±0.21b | 7.88±0.35b | 9.16±0.32a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 8.81±0.76a | 9.39±0.25b | 7.97±0.11c | 6.47±0.27c | 5.33±0.11d | 6.48±0.10d |
5 | 8.26±0.13a | 9.51±0.23b | 8.89±0.11b | 7.47±0.23b | 6.86±0.18c | 8.45±0.36c | |
10 | 9.36±0.63a | 10.59±0.26a | 9.47±0.12a | 8.42±0.25a | 7.67±0.16a | 10.26±0.20a | |
15 | 9.57±0.92a | 10.20±0.35a | 8.54±0.41b | 7.83±0.21b | 7.34±0.17b | 9.31±0.06b |
表9 低温胁迫下外源物质施用对柱花草可溶性蛋白含量的影响
Table 9 Effects of exogenous substances application on soluble protein content of S. guianensis under low temperature stress
外源物质 Exogenous substances | 浓度 Concentration | 可溶性蛋白含量Soluble protein content (mg·g-1) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
壳聚糖 Chitosan (mg·L-1) | 0 | 5.61±0.36a | 5.54±0.25c | 4.70±0.41c | 3.50±0.29c | 3.42±0.05d | 4.53±0.35b |
300 | 5.47±1.14a | 7.31±0.29a | 6.92±0.34a | 6.40±0.04a | 5.54±0.07a | 6.60±0.32a | |
400 | 5.27±1.20a | 7.34±0.27a | 6.65±0.23a | 6.22±0.21a | 5.24±0.19b | 6.40±0.20a | |
500 | 5.73±0.28a | 6.61±0.38b | 6.02±0.21b | 5.37±0.42b | 4.89±0.16c | 6.70±0.36a | |
水杨酸 Salicylic acid (mmol·L-1) | 0 | 8.49±1.26a | 9.58±0.38c | 8.42±0.34c | 6.47±0.27d | 5.33±0.11d | 6.48±0.10b |
0.2 | 9.34±0.89a | 10.61±0.41b | 9.70±0.26b | 8.33±0.20c | 6.75±0.41c | 9.01±0.21a | |
0.4 | 9.84±0.98a | 11.33±0.28a | 10.28±0.05a | 9.42±0.13a | 8.66±0.05a | 9.07±0.33a | |
0.6 | 9.80±0.40a | 10.67±0.29b | 9.34±0.19b | 8.78±0.21b | 7.88±0.35b | 9.16±0.32a | |
脱落酸 Abscisic acid (mg·L-1) | 0 | 8.81±0.76a | 9.39±0.25b | 7.97±0.11c | 6.47±0.27c | 5.33±0.11d | 6.48±0.10d |
5 | 8.26±0.13a | 9.51±0.23b | 8.89±0.11b | 7.47±0.23b | 6.86±0.18c | 8.45±0.36c | |
10 | 9.36±0.63a | 10.59±0.26a | 9.47±0.12a | 8.42±0.25a | 7.67±0.16a | 10.26±0.20a | |
15 | 9.57±0.92a | 10.20±0.35a | 8.54±0.41b | 7.83±0.21b | 7.34±0.17b | 9.31±0.06b |
图1 3种外源物质处理对柱花草Fv/Fm和Fv/F0的影响R2: 低温胁迫结束后2 d。2 days after low temperature stress.下同The same below.
Fig.1 Effects of three exogenous substances on Fv/Fm and Fv/F0 of S. guianensis
荧光参数 Fluorescence parameters | 外源物质 Exogenous substances | 低温处理时间 Low temperature treatment time (d) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
VJ | 对照CK | 0.32±0.01b | 0.57±0.01a | 0.67±0.01a | 0.77±0.01a | 0.81±0.01a | 0.57±0.01a |
0.4 mmol·L-1水杨酸Salicylic acid | 0.35±0.01a | 0.37±0.01c | 0.46±0.02b | 0.56±0.01b | 0.59±0.01b | 0.56±0.02a | |
10 mg·L-1脱落酸Abscisic acid | 0.42±0.01b | 0.42±0.01b | 0.43±0.01c | 0.51±0.01c | 0.54±0.01c | 0.37±0.02b | |
300 mg·L-1壳聚糖Chitosan | 0.38±0.02c | 0.38±0.02c | 0.41±0.01d | 0.45±0.02d | 0.47±0.01d | 0.37±0.01b | |
ψ0 | 对照CK | 0.62±0.00c | 0.46±0.00b | 0.43±0.01d | 0.33±0.01c | 0.27±0.01d | 0.44±0.01c |
0.4 mmol·L-1水杨酸Salicylic acid | 0.65±0.01b | 0.62±0.02a | 0.55±0.02c | 0.52±0.02b | 0.47±0.01c | 0.58±0.02b | |
10 mg·L-1脱落酸Abscisic acid | 0.64±0.01b | 0.62±0.03a | 0.57±0.02b | 0.55±0.02a | 0.50±0.01b | 0.65±0.02a | |
300 mg·L-1壳聚糖Chitosan | 0.68±0.01a | 0.62±0.02a | 0.60±0.01a | 0.56±0.00a | 0.53±0.01a | 0.63±0.02a | |
φE0 | 对照CK | 0.57±0.01a | 0.30±0.01c | 0.28±0.00b | 0.22±0.00d | 0.15±0.00d | 0.40±0.01b |
0.4 mmol·L-1水杨酸Salicylic acid | 0.55±0.02b | 0.50±0.01a | 0.45±0.01a | 0.37±0.01c | 0.33±0.03c | 0.40±0.02b | |
10 mg·L-1脱落酸Abscisic acid | 0.55±0.01b | 0.47±0.02b | 0.46±0.02a | 0.41±0.01b | 0.38±0.02b | 0.54±0.02a | |
300 mg·L-1壳聚糖Chitosan | 0.58±0.01a | 0.50±0.02a | 0.46±0.01a | 0.43±0.01a | 0.41±0.01a | 0.53±0.02a | |
φD0 | 对照CK | 0.15±0.01a | 0.34±0.02a | 0.44±0.00a | 0.51±0.01a | 0.63±0.00a | 0.33±0.01a |
0.4 mmol·L-1水杨酸Salicylic acid | 0.15±0.01a | 0.23±0.01b | 0.31±0.01b | 0.37±0.02b | 0.46±0.02b | 0.27±0.01b | |
10 mg·L-1脱落酸Abscisic acid | 0.14±0.00a | 0.23±0.03b | 0.28±0.02c | 0.34±0.01c | 0.45±0.01b | 0.28±0.02b | |
300 mg·L-1壳聚糖Chitosan | 0.14±0.00a | 0.19±0.01c | 0.26±0.02d | 0.34±0.02c | 0.40±0.02c | 0.27±0.03b |
表10 低温胁迫下外源物质施用对柱花草各荧光参数的影响
Table 10 Effects of exogenous substances application on fluorescence parameters of S. guianensis under low temperature stress
荧光参数 Fluorescence parameters | 外源物质 Exogenous substances | 低温处理时间 Low temperature treatment time (d) | |||||
---|---|---|---|---|---|---|---|
d0 | d1 | d3 | d5 | d7 | R2 | ||
VJ | 对照CK | 0.32±0.01b | 0.57±0.01a | 0.67±0.01a | 0.77±0.01a | 0.81±0.01a | 0.57±0.01a |
0.4 mmol·L-1水杨酸Salicylic acid | 0.35±0.01a | 0.37±0.01c | 0.46±0.02b | 0.56±0.01b | 0.59±0.01b | 0.56±0.02a | |
10 mg·L-1脱落酸Abscisic acid | 0.42±0.01b | 0.42±0.01b | 0.43±0.01c | 0.51±0.01c | 0.54±0.01c | 0.37±0.02b | |
300 mg·L-1壳聚糖Chitosan | 0.38±0.02c | 0.38±0.02c | 0.41±0.01d | 0.45±0.02d | 0.47±0.01d | 0.37±0.01b | |
ψ0 | 对照CK | 0.62±0.00c | 0.46±0.00b | 0.43±0.01d | 0.33±0.01c | 0.27±0.01d | 0.44±0.01c |
0.4 mmol·L-1水杨酸Salicylic acid | 0.65±0.01b | 0.62±0.02a | 0.55±0.02c | 0.52±0.02b | 0.47±0.01c | 0.58±0.02b | |
10 mg·L-1脱落酸Abscisic acid | 0.64±0.01b | 0.62±0.03a | 0.57±0.02b | 0.55±0.02a | 0.50±0.01b | 0.65±0.02a | |
300 mg·L-1壳聚糖Chitosan | 0.68±0.01a | 0.62±0.02a | 0.60±0.01a | 0.56±0.00a | 0.53±0.01a | 0.63±0.02a | |
φE0 | 对照CK | 0.57±0.01a | 0.30±0.01c | 0.28±0.00b | 0.22±0.00d | 0.15±0.00d | 0.40±0.01b |
0.4 mmol·L-1水杨酸Salicylic acid | 0.55±0.02b | 0.50±0.01a | 0.45±0.01a | 0.37±0.01c | 0.33±0.03c | 0.40±0.02b | |
10 mg·L-1脱落酸Abscisic acid | 0.55±0.01b | 0.47±0.02b | 0.46±0.02a | 0.41±0.01b | 0.38±0.02b | 0.54±0.02a | |
300 mg·L-1壳聚糖Chitosan | 0.58±0.01a | 0.50±0.02a | 0.46±0.01a | 0.43±0.01a | 0.41±0.01a | 0.53±0.02a | |
φD0 | 对照CK | 0.15±0.01a | 0.34±0.02a | 0.44±0.00a | 0.51±0.01a | 0.63±0.00a | 0.33±0.01a |
0.4 mmol·L-1水杨酸Salicylic acid | 0.15±0.01a | 0.23±0.01b | 0.31±0.01b | 0.37±0.02b | 0.46±0.02b | 0.27±0.01b | |
10 mg·L-1脱落酸Abscisic acid | 0.14±0.00a | 0.23±0.03b | 0.28±0.02c | 0.34±0.01c | 0.45±0.01b | 0.28±0.02b | |
300 mg·L-1壳聚糖Chitosan | 0.14±0.00a | 0.19±0.01c | 0.26±0.02d | 0.34±0.02c | 0.40±0.02c | 0.27±0.03b |
外源物质 Exogenous substances | 浓度 Concentration | Chl | Carotenoids | REC | MDA | POD | CAT | SOD | SP | CI | MFM |
---|---|---|---|---|---|---|---|---|---|---|---|
水杨酸 Salicylic acid (mmol·L-1) | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
0.2 | 0.58 | 0.64 | 0.76 | 0.75 | 0.59 | 0.39 | 0.49 | 0.43 | 0.62 | 0.58 | |
0.4 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
0.6 | 0.76 | 0.33 | 0.48 | 0.45 | 0.74 | 0.59 | 0.63 | 0.77 | 0.81 | 0.62 | |
脱落酸 Abscisic acid (mg·L-1) | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
5 | 0.43 | 0.52 | 0.79 | 0.69 | 0.70 | 0.64 | 0.39 | 0.65 | 0.94 | 0.64 | |
10 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
15 | 0.70 | 0.76 | 0.58 | 0.84 | 0.31 | 0.79 | 0.59 | 0.86 | 0.74 | 0.69 | |
壳聚糖 Chitosan (mg·L-1) | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
300 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
400 | 0.76 | 0.78 | 0.79 | 0.56 | 0.54 | 0.78 | 0.59 | 0.86 | 0.89 | 0.73 | |
500 | 0.55 | 0.51 | 0.43 | 0.81 | 0.77 | 0.56 | 0.43 | 0.69 | 0.85 | 0.62 |
表11 不同浓度外源物质处理柱花草生理指标的隶属函数值
Table 11 Membership function values ??of physiological indexes of S. guianensis in different concentrations of exogenous substances
外源物质 Exogenous substances | 浓度 Concentration | Chl | Carotenoids | REC | MDA | POD | CAT | SOD | SP | CI | MFM |
---|---|---|---|---|---|---|---|---|---|---|---|
水杨酸 Salicylic acid (mmol·L-1) | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
0.2 | 0.58 | 0.64 | 0.76 | 0.75 | 0.59 | 0.39 | 0.49 | 0.43 | 0.62 | 0.58 | |
0.4 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
0.6 | 0.76 | 0.33 | 0.48 | 0.45 | 0.74 | 0.59 | 0.63 | 0.77 | 0.81 | 0.62 | |
脱落酸 Abscisic acid (mg·L-1) | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
5 | 0.43 | 0.52 | 0.79 | 0.69 | 0.70 | 0.64 | 0.39 | 0.65 | 0.94 | 0.64 | |
10 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
15 | 0.70 | 0.76 | 0.58 | 0.84 | 0.31 | 0.79 | 0.59 | 0.86 | 0.74 | 0.69 | |
壳聚糖 Chitosan (mg·L-1) | CK | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
300 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
400 | 0.76 | 0.78 | 0.79 | 0.56 | 0.54 | 0.78 | 0.59 | 0.86 | 0.89 | 0.73 | |
500 | 0.55 | 0.51 | 0.43 | 0.81 | 0.77 | 0.56 | 0.43 | 0.69 | 0.85 | 0.62 |
外源物质Exogenous substances | Fv/Fm | Fv/F0 | VJ | ψ0 | φD0 | φE0 | ABS/RC | TR0/RC | ET0/RC | DI0/RC | PIABS | MFM |
---|---|---|---|---|---|---|---|---|---|---|---|---|
0.4 mmol·L-1水杨酸Salicylic acid | 0.04 | 0.08 | 0.09 | 0.16 | 0.16 | 0.37 | 0.08 | 0.11 | 0.12 | 0.03 | 0.08 | 0.12 |
10 mg·L-1脱落酸Abscisic acid | 0.48 | 0.35 | 0.44 | 0.52 | 0.31 | 0.66 | 0.41 | 0.83 | 0.34 | 0.29 | 0.53 | 0.47 |
300 mg·L-1壳聚糖Chitosan | 0.90 | 0.99 | 0.93 | 0.84 | 0.74 | 0.89 | 0.76 | 0.26 | 0.90 | 0.94 | 0.95 | 0.83 |
表12 最佳浓度下柱花草叶绿素荧光参数的隶属函数值
Table 12 Membership function values of chlorophyll fluorescence parameters of S. guianensis at the optimal concentration treatment
外源物质Exogenous substances | Fv/Fm | Fv/F0 | VJ | ψ0 | φD0 | φE0 | ABS/RC | TR0/RC | ET0/RC | DI0/RC | PIABS | MFM |
---|---|---|---|---|---|---|---|---|---|---|---|---|
0.4 mmol·L-1水杨酸Salicylic acid | 0.04 | 0.08 | 0.09 | 0.16 | 0.16 | 0.37 | 0.08 | 0.11 | 0.12 | 0.03 | 0.08 | 0.12 |
10 mg·L-1脱落酸Abscisic acid | 0.48 | 0.35 | 0.44 | 0.52 | 0.31 | 0.66 | 0.41 | 0.83 | 0.34 | 0.29 | 0.53 | 0.47 |
300 mg·L-1壳聚糖Chitosan | 0.90 | 0.99 | 0.93 | 0.84 | 0.74 | 0.89 | 0.76 | 0.26 | 0.90 | 0.94 | 0.95 | 0.83 |
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