Identification， evolution and expression analysis of the CCoAOMT family genes in Medicago sativa

doi:10.11686/cyxb2020429

Abstract

Abstract:

Alfalfa （Medicago sativa） is a forage legume with high feed quality widely cultivated all over the world. It contains lignins that play an important role in stress resistance of alfalfa. However， lignin seriously affects the nutritional value of alfalfa because it is difficult for livestock to digest. Therefore， it is necessary to breed alfalfa varieties with low lignin content. Caffeoyl CoA O-methyltransferase （CCoAOMT） is a key enzyme in the lignin synthesis pathway. In this study， the members of CCoAOMT gene family in the alfalfa genome were identified by bioinformatic methods， and the gene structure， chromosome location， phylogeny and gene expression were analysed. It was found that there were 44 MsCCoAOMT genes in the alfalfa genome. These genes were distributed on 16 chromosomes with tandem duplication， among which 36 （82%） MsCCoAOMT genes contained 5 exons. A phylogenetic tree was constructed and showed that MsCCoAOMT family genes can be classified into 5 groups. In addition， ten conserved motifs were found by predicting the motif of MsCCoAOMT protein with MEME software. qRT-PCR results indicated that most of MsCCoAOMT genes were tissue-specific in their expression. Our results provide reference information for the genetic improvement of alfalfa and development of lines with low lignin content.

Key words: Medicago sativa, lignin, caffeoyl CoA O-methyltransferase, gene family, gene expression

Qian MA, Qi YAN, Zheng-she ZHANG, Fan WU, Ji-yu ZHANG. Identification， evolution and expression analysis of the CCoAOMT family genes in Medicago sativa[J]. Acta Prataculturae Sinica, 2021, 30(11): 144-156.

Figures/Tables 8

References 32

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基因名称 Gene name	引物序列 Primer sequence （5'-3'）	基因名称 Gene name	引物序列 Primer sequence （5'-3'）
MsCCoAOMT1	F： TGCTGATCCATTGGTAAGTGAC	MsCCoAOMT25	F： CTATACCCGGTGATGAAGGAC
MsCCoAOMT1	R： TTGTCATCCCATCTCCAATAGGT	MsCCoAOMT25	R： CAAAGCCAATACCTTTCCATCAG
MsCCoAOMT10	F： TTCATGGGCACTTCACCT	MsCCoAOMT32	F： CTATACCCGCTGATGAAGGAC
MsCCoAOMT10	R： GGCTGTAATCTTTCCATCATATGG	MsCCoAOMT32	R： AGCCAACACCTTTCCATCCT
MsCCoAOMT12	F： AAGCAAACTGAATCTGGAAGAC	MsCCoAOMT41	F： GGAACCTTATGGCTACACCT
MsCCoAOMT12	R： TTGTCATGATGTTCCATGGGT	MsCCoAOMT41	R： AAGCCAATACCTTTCCATCAG
MsCCoAOMT17	F： TGCCTTTGGATGAAGCAC	MsCCoAOMT43	F： CACCCATGCTTGAAGGAG
MsCCoAOMT17	R： CAATTCCTATTACCTTGCCATCAG	MsCCoAOMT43	R： AGCTAACACCTTTCCATCCT
MsCCoAOMT21	F： GGCTGGTCAACTAATCACTC	MsCCoAOMT44	F： CTAATGGGCATACTCTTGAAGCT
MsCCoAOMT21	R： GGCTGTAATCTTTCCATCGT	MsCCoAOMT44	R： AGCTATGATCTTTCCATCATCAGG
MsCCoAOMT23	F： GAGATGACACTATCACACCCT	MsActin	F： GACAATGGAACTGGAATGG
MsCCoAOMT23	R： AAGCCAATACCTTTCCATCAG	MsActin	R： CAATACCGTGCTCAATGG

基因名称 Gene name	引物序列 Primer sequence （5'-3'）	基因名称 Gene name	引物序列 Primer sequence （5'-3'）
MsCCoAOMT1	F： TGCTGATCCATTGGTAAGTGAC	MsCCoAOMT25	F： CTATACCCGGTGATGAAGGAC
MsCCoAOMT1	R： TTGTCATCCCATCTCCAATAGGT	MsCCoAOMT25	R： CAAAGCCAATACCTTTCCATCAG
MsCCoAOMT10	F： TTCATGGGCACTTCACCT	MsCCoAOMT32	F： CTATACCCGCTGATGAAGGAC
MsCCoAOMT10	R： GGCTGTAATCTTTCCATCATATGG	MsCCoAOMT32	R： AGCCAACACCTTTCCATCCT
MsCCoAOMT12	F： AAGCAAACTGAATCTGGAAGAC	MsCCoAOMT41	F： GGAACCTTATGGCTACACCT
MsCCoAOMT12	R： TTGTCATGATGTTCCATGGGT	MsCCoAOMT41	R： AAGCCAATACCTTTCCATCAG
MsCCoAOMT17	F： TGCCTTTGGATGAAGCAC	MsCCoAOMT43	F： CACCCATGCTTGAAGGAG
MsCCoAOMT17	R： CAATTCCTATTACCTTGCCATCAG	MsCCoAOMT43	R： AGCTAACACCTTTCCATCCT
MsCCoAOMT21	F： GGCTGGTCAACTAATCACTC	MsCCoAOMT44	F： CTAATGGGCATACTCTTGAAGCT
MsCCoAOMT21	R： GGCTGTAATCTTTCCATCGT	MsCCoAOMT44	R： AGCTATGATCTTTCCATCATCAGG
MsCCoAOMT23	F： GAGATGACACTATCACACCCT	MsActin	F： GACAATGGAACTGGAATGG
MsCCoAOMT23	R： AAGCCAATACCTTTCCATCAG	MsActin	R： CAATACCGTGCTCAATGG

基因名称 Gene name	基因座 Gene ID	蛋白质大小 Protein length （aa）	分子量MW （kDa）	等电点 pI	疏水性指数 GRAVY	基因序列长度Length of intron and exon （bp）	亚细胞定位Subcellular localization	拟南芥同源基因 Homologous gene of Arabidopsis
MsCCoAOMT1	MS.gene039184.t1	309	34.3566	8.54	-0.123	5315	Cytoplasm	AT3G62000.1
MsCCoAOMT2	MS.gene068675.t1	293	32.6967	8.58	-0.083	3724	Chloroplast	AT3G62000.1
MsCCoAOMT3	MS.gene063440.t1	293	32.7298	8.58	-0.061	3345	Chloroplast	AT3G62000.1
MsCCoAOMT4	MS.gene062840.t1	267	29.9507	8.58	-0.080	7396	Chloroplast	AT3G62000.1
MsCCoAOMT5	MS.gene049025.t1	251	27.9613	5.51	0.020	1473	Chloroplast	AT4G26220.1
MsCCoAOMT6	MS.gene26463.t1	247	27.9992	5.55	-0.308	1302	Cytoplasm	AT4G34050.1
MsCCoAOMT7	MS.gene06173.t1	235	26.1382	5.51	-0.023	1495	Chloroplast	AT4G26220.1
MsCCoAOMT8	MS.gene058635.t1	133	14.8534	5.59	0.041	724	N and C	AT1G67980.1
MsCCoAOMT9	MS.gene028389.t1	229	25.7237	5.14	-0.114	2386	Chloroplast	AT1G67980.1
MsCCoAOMT10	MS.gene55886.t1	235	26.0962	5.51	-0.043	1495	Chloroplast	AT4G26220.1
MsCCoAOMT11	MS.gene28756.t1	229	25.7418	5.23	-0.097	1842	Chloroplast	AT1G67980.1
MsCCoAOMT12	MS.gene059423.t1	247	27.9992	5.55	-0.308	1303	Cytoplasm	AT4G34050.1
MsCCoAOMT13	MS.gene053535.t1	247	27.9992	5.55	-0.308	1303	Cytoplasm	AT4G34050.1
MsCCoAOMT14	MS.gene053539.t1	248	28.0291	5.41	-0.280	1303	Cytoplasm	AT4G34050.1
MsCCoAOMT15	MS.gene053537.t1	182	20.5040	6.31	-0.066	881	Cytoplasm	AT4G34050.1
MsCCoAOMT16	MS.gene20157.t1	235	26.1222	5.51	-0.023	1492	Chloroplast	AT4G26220.1
MsCCoAOMT17	MS.gene35069.t1	229	25.7377	5.14	-0.112	2454	Chloroplast	AT1G67980.1
MsCCoAOMT18	MS.gene047856.t1	247	27.9992	5.55	-0.308	1322	Cytoplasm	AT4G34050.1
MsCCoAOMT19	MS.gene064029.t1	238	26.8580	5.23	-0.174	2072	Cytoplasm	AT4G26220.1
MsCCoAOMT20	MS.gene030303.t1	238	26.8720	5.35	-0.175	2087	Cytoplasm	AT4G26220.1
MsCCoAOMT21	MS.gene75055.t1	238	26.9041	5.35	-0.182	2042	Cytoplasm	AT4G26220.1
MsCCoAOMT22	MS.gene66349.t1	238	26.8580	5.23	-0.174	2072	Cytoplasm	AT4G26220.1
MsCCoAOMT23	MS.gene44534.t1	238	26.7568	5.58	-0.132	1866	Cytoplasm	AT4G34050.1
MsCCoAOMT24	MS.gene44535.t1	568	63.5446	4.89	-0.170	4423	Chloroplast	AT2G23250.1
MsCCoAOMT25	MS.gene011784.t1	248	27.9000	5.15	-0.173	3149	Cytoplasm	AT4G34050.1
MsCCoAOMT26	MS.gene011803.t1	248	27.8408	4.99	-0.198	3883	Cytoplasm	AT4G34050.1
MsCCoAOMT27	MS.gene011804.t1	246	27.7320	5.50	-0.130	1624	Cytoplasm	AT4G34050.1
MsCCoAOMT28	MS.gene30425.t1	187	20.9131	5.22	-0.100	2679	Cytoplasm	AT4G26220.1
MsCCoAOMT29	MS.gene011778.t1	238	26.7828	5.48	-0.124	2777	Cytoplasm	AT4G34050.1
MsCCoAOMT30	MS.gene56811.t1	248	27.8449	5.06	-0.158	4031	Cytoplasm	AT4G34050.1
MsCCoAOMT31	MS.gene56793.t1	248	27.8689	4.99	-0.185	3744	Cytoplasm	AT4G34050.1
MsCCoAOMT32	MS.gene56792.t1	189	21.6996	5.65	0.286	1330	Cytoplasm	AT4G34050.1
MsCCoAOMT33	MS.gene074442.t1	235	26.3723	5.52	-0.197	3568	Cytoplasm	AT4G26220.1
MsCCoAOMT34	MS.gene90469.t1	238	26.7828	5.48	-0.124	1850	Cytoplasm	AT4G34050.1
MsCCoAOMT35	MS.gene90479.t1	248	27.9000	5.15	-0.173	2947	Cytoplasm	AT4G34050.1
MsCCoAOMT36	MS.gene90498.t1	248	27.8127	4.91	-0.182	3043	Cytoplasm	AT4G34050.1
MsCCoAOMT37	MS.gene90499.t1	246	27.7240	5.50	-0.108	1637	Cytoplasm	AT4G34050.1
MsCCoAOMT38	MS.gene051092.t1	235	26.4173	5.30	-0.186	3565	Cytoplasm	AT4G26220.1
MsCCoAOMT39	MS.gene041940.t1	238	26.7828	5.48	-0.124	2498	Cytoplasm	AT4G34050.1
MsCCoAOMT40	MS.gene041950.t1	248	27.9071	5.16	-0.160	2984	Cytoplasm	AT4G34050.1
MsCCoAOMT41	MS.gene99216.t1	248	27.8408	4.99	-0.195	3776	Cytoplasm	AT4G34050.1
MsCCoAOMT42	MS.gene99215.t1	246	27.7351	5.50	-0.034	1458	Cytoplasm	AT4G34050.1
MsCCoAOMT43	MS.gene99213.t1	246	27.7460	5.50	-0.129	1637	Cytoplasm	AT4G34050.1
MsCCoAOMT44	MS.gene25100.t1	235	26.4394	5.55	-0.185	3584	Cytoplasm	AT4G26220.1

基因名称 Gene name	基因座 Gene ID	蛋白质大小 Protein length （aa）	分子量MW （kDa）	等电点 pI	疏水性指数 GRAVY	基因序列长度Length of intron and exon （bp）	亚细胞定位Subcellular localization	拟南芥同源基因 Homologous gene of Arabidopsis
MsCCoAOMT1	MS.gene039184.t1	309	34.3566	8.54	-0.123	5315	Cytoplasm	AT3G62000.1
MsCCoAOMT2	MS.gene068675.t1	293	32.6967	8.58	-0.083	3724	Chloroplast	AT3G62000.1
MsCCoAOMT3	MS.gene063440.t1	293	32.7298	8.58	-0.061	3345	Chloroplast	AT3G62000.1
MsCCoAOMT4	MS.gene062840.t1	267	29.9507	8.58	-0.080	7396	Chloroplast	AT3G62000.1
MsCCoAOMT5	MS.gene049025.t1	251	27.9613	5.51	0.020	1473	Chloroplast	AT4G26220.1
MsCCoAOMT6	MS.gene26463.t1	247	27.9992	5.55	-0.308	1302	Cytoplasm	AT4G34050.1
MsCCoAOMT7	MS.gene06173.t1	235	26.1382	5.51	-0.023	1495	Chloroplast	AT4G26220.1
MsCCoAOMT8	MS.gene058635.t1	133	14.8534	5.59	0.041	724	N and C	AT1G67980.1
MsCCoAOMT9	MS.gene028389.t1	229	25.7237	5.14	-0.114	2386	Chloroplast	AT1G67980.1
MsCCoAOMT10	MS.gene55886.t1	235	26.0962	5.51	-0.043	1495	Chloroplast	AT4G26220.1
MsCCoAOMT11	MS.gene28756.t1	229	25.7418	5.23	-0.097	1842	Chloroplast	AT1G67980.1
MsCCoAOMT12	MS.gene059423.t1	247	27.9992	5.55	-0.308	1303	Cytoplasm	AT4G34050.1
MsCCoAOMT13	MS.gene053535.t1	247	27.9992	5.55	-0.308	1303	Cytoplasm	AT4G34050.1
MsCCoAOMT14	MS.gene053539.t1	248	28.0291	5.41	-0.280	1303	Cytoplasm	AT4G34050.1
MsCCoAOMT15	MS.gene053537.t1	182	20.5040	6.31	-0.066	881	Cytoplasm	AT4G34050.1
MsCCoAOMT16	MS.gene20157.t1	235	26.1222	5.51	-0.023	1492	Chloroplast	AT4G26220.1
MsCCoAOMT17	MS.gene35069.t1	229	25.7377	5.14	-0.112	2454	Chloroplast	AT1G67980.1
MsCCoAOMT18	MS.gene047856.t1	247	27.9992	5.55	-0.308	1322	Cytoplasm	AT4G34050.1
MsCCoAOMT19	MS.gene064029.t1	238	26.8580	5.23	-0.174	2072	Cytoplasm	AT4G26220.1
MsCCoAOMT20	MS.gene030303.t1	238	26.8720	5.35	-0.175	2087	Cytoplasm	AT4G26220.1
MsCCoAOMT21	MS.gene75055.t1	238	26.9041	5.35	-0.182	2042	Cytoplasm	AT4G26220.1
MsCCoAOMT22	MS.gene66349.t1	238	26.8580	5.23	-0.174	2072	Cytoplasm	AT4G26220.1
MsCCoAOMT23	MS.gene44534.t1	238	26.7568	5.58	-0.132	1866	Cytoplasm	AT4G34050.1
MsCCoAOMT24	MS.gene44535.t1	568	63.5446	4.89	-0.170	4423	Chloroplast	AT2G23250.1
MsCCoAOMT25	MS.gene011784.t1	248	27.9000	5.15	-0.173	3149	Cytoplasm	AT4G34050.1
MsCCoAOMT26	MS.gene011803.t1	248	27.8408	4.99	-0.198	3883	Cytoplasm	AT4G34050.1
MsCCoAOMT27	MS.gene011804.t1	246	27.7320	5.50	-0.130	1624	Cytoplasm	AT4G34050.1
MsCCoAOMT28	MS.gene30425.t1	187	20.9131	5.22	-0.100	2679	Cytoplasm	AT4G26220.1
MsCCoAOMT29	MS.gene011778.t1	238	26.7828	5.48	-0.124	2777	Cytoplasm	AT4G34050.1
MsCCoAOMT30	MS.gene56811.t1	248	27.8449	5.06	-0.158	4031	Cytoplasm	AT4G34050.1
MsCCoAOMT31	MS.gene56793.t1	248	27.8689	4.99	-0.185	3744	Cytoplasm	AT4G34050.1
MsCCoAOMT32	MS.gene56792.t1	189	21.6996	5.65	0.286	1330	Cytoplasm	AT4G34050.1
MsCCoAOMT33	MS.gene074442.t1	235	26.3723	5.52	-0.197	3568	Cytoplasm	AT4G26220.1
MsCCoAOMT34	MS.gene90469.t1	238	26.7828	5.48	-0.124	1850	Cytoplasm	AT4G34050.1
MsCCoAOMT35	MS.gene90479.t1	248	27.9000	5.15	-0.173	2947	Cytoplasm	AT4G34050.1
MsCCoAOMT36	MS.gene90498.t1	248	27.8127	4.91	-0.182	3043	Cytoplasm	AT4G34050.1
MsCCoAOMT37	MS.gene90499.t1	246	27.7240	5.50	-0.108	1637	Cytoplasm	AT4G34050.1
MsCCoAOMT38	MS.gene051092.t1	235	26.4173	5.30	-0.186	3565	Cytoplasm	AT4G26220.1
MsCCoAOMT39	MS.gene041940.t1	238	26.7828	5.48	-0.124	2498	Cytoplasm	AT4G34050.1
MsCCoAOMT40	MS.gene041950.t1	248	27.9071	5.16	-0.160	2984	Cytoplasm	AT4G34050.1
MsCCoAOMT41	MS.gene99216.t1	248	27.8408	4.99	-0.195	3776	Cytoplasm	AT4G34050.1
MsCCoAOMT42	MS.gene99215.t1	246	27.7351	5.50	-0.034	1458	Cytoplasm	AT4G34050.1
MsCCoAOMT43	MS.gene99213.t1	246	27.7460	5.50	-0.129	1637	Cytoplasm	AT4G34050.1
MsCCoAOMT44	MS.gene25100.t1	235	26.4394	5.55	-0.185	3584	Cytoplasm	AT4G26220.1

基因名称 Gene name	元件名称Element name
	基本核心元件 Basic core components				光信号感知顺式作用元件Optical signal sensing cis-acting element						激素响应元件 Hormone response element									生物应激反应元件 Biological stress response element					蛋白质结合元件 Protein binding element				组织发育元件 Tissue development element
	X₁	X₂	X₃	X₄	X₅	X₆	X₇	X₈	X₉	X₁₀	X₁₁	X₁₂	X₁₃	X₁₄	X₁₅	X₁₆	X₁₇	X₁₈	X₁₉	X₂₀	X₂₁	X₂₂	X₂₃	X₂₄	X₂₅	X₂₆	X₂₇	X₂₈	X₂₉	X₃₀	X₃₁	X₃₂
Ms₁	46	24	2	1	0	2	1	0	0	0	1	0	1	0	0	1	2	0	1	3	0	0	0	0	0	0	0	0	1	0	0	0
Ms₂	34	22	1	0	1	3	1	1	2	1	3	0	0	2	1	1	1	2	0	1	0	0	0	0	0	0	0	0	0	0	0	0
Ms₃	43	14	3	2	3	1	4	0	0	0	1	0	0	2	1	1	1	2	0	2	0	0	1	0	0	0	0	0	0	0	0	0
Ms₄	33	11	0	0	2	2	2	0	2	0	3	0	0	1	2	1	1	1	0	0	0	0	1	0	0	1	0	1	0	1	0	0
Ms₅	35	39	1	0	3	1	2	0	0	2	0	1	1	0	0	0	0	0	0	1	0	0	0	1	0	1	0	0	2	0	0	0
Ms₆	32	47	1	0	2	4	1	2	0	0	2	0	0	1	0	1	0	1	0	3	1	1	1	1	0	0	0	0	0	0	0	1
Ms₇	35	39	1	0	3	1	2	0	0	2	0	1	1	0	0	0	0	0	0	1	0	0	0	1	0	1	0	0	2	0	0	0
Ms₈	41	51	1	0	2	4	3	1	0	0	3	0	1	1	1	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	0	0
Ms₉	39	56	1	0	2	4	3	1	0	0	2	0	1	1	1	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	0	0
Ms₁₀	35	39	1	0	3	1	2	0	0	2	0	1	1	0	0	0	0	0	0	1	0	0	0	1	0	1	0	0	2	0	0	0
Ms₁₁	38	54	0	0	4	1	3	2	0	0	1	0	1	2	1	0	0	2	0	1	0	3	1	0	1	0	0	3	0	0	0	0
Ms₁₂	31	43	1	0	2	3	2	2	0	0	1	0	0	1	0	1	0	1	0	2	2	1	1	0	0	0	0	0	0	0	0	1
Ms₁₃	33	47	1	0	2	3	2	2	0	0	1	0	0	1	0	1	0	1	0	2	2	1	1	0	0	0	0	0	0	0	0	1
Ms₁₄	23	75	0	0	2	2	2	2	0	0	1	0	0	1	0	2	0	1	0	2	0	1	0	0	0	0	0	0	0	0	0	1
Ms₁₅	43	36	0	0	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	2	0	0	1	0	0	0	0
Ms₁₆	35	39	1	0	3	1	2	0	0	2	0	1	1	0	0	0	0	0	0	1	0	0	0	1	0	1	0	0	2	0	0	0
Ms₁₇	39	56	1	0	2	4	3	1	0	0	2	0	1	1	1	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	0	0
Ms₁₈	32	48	1	0	2	3	2	1	0	0	1	0	0	1	0	1	0	1	0	2	2	1	0	1	0	0	0	0	0	0	0	1
Ms₁₉	44	60	0	0	3	3	0	0	1	1	3	0	0	1	0	0	0	1	0	3	0	2	0	3	0	0	0	0	0	0	0	0
Ms₂₀	39	43	0	0	5	2	0	0	0	0	2	0	0	2	0	0	0	2	0	2	0	1	0	1	1	0	0	0	0	1	0	0
Ms₂₁	43	60	0	0	2	3	0	0	1	1	3	0	0	1	0	0	0	1	0	3	0	1	0	2	0	0	0	0	0	0	0	0
Ms₂₂	44	61	0	0	2	3	0	0	1	1	3	0	0	1	0	0	0	1	0	2	0	1	0	2	0	0	0	0	0	0	0	0
Ms₂₃	41	25	1	1	4	2	3	1	0	0	3	0	0	1	1	1	1	1	0	2	0	0	1	0	0	0	0	0	1	0	0	0
Ms₂₄	38	33	0	0	2	1	1	1	0	0	0	0	2	2	0	0	1	2	0	3	0	0	1	1	0	0	0	0	2	0	0	0
Ms₂₅	39	60	1	1	1	0	2	1	0	0	0	1	0	3	0	0	0	3	0	0	0	0	0	1	0	1	0	0	2	0	1	0
Ms₂₆	29	21	0	0	0	2	2	0	0	1	0	0	2	1	0	0	1	1	0	2	0	2	1	0	0	1	0	3	1	0	0	0
Ms₂₇	39	53	0	0	4	2	1	1	1	0	3	0	1	1	0	1	0	1	0	2	0	1	2	0	0	0	0	0	0	0	0	0
Ms₂₈	53	46	1	1	5	4	3	0	0	0	2	0	1	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	1	1	0	0
Ms₂₉	40	27	1	1	4	2	3	1	0	0	3	0	0	1	0	1	1	1	0	2	0	0	1	1	0	0	0	0	1	0	0	0
Ms₃₀	37	66	1	1	1	0	2	1	0	0	0	1	0	3	0	0	0	3	0	0	0	0	0	1	0	1	0	0	2	0	1	0
Ms₃₁	39	56	0	0	3	2	0	0	0	0	3	0	0	1	1	0	0	1	0	0	0	0	1	0	1	1	0	2	0	0	0	0
Ms₃₂	47	43	0	1	0	1	1	0	0	0	1	0	1	1	0	0	2	0	0	4	0	0	1	0	0	0	0	2	0	0	0	0
Ms₃₃	46	28	0	0	2	3	2	1	0	0	2	0	2	0	1	0	0	0	0	4	0	3	0	0	0	1	0	0	0	2	0	0
Ms₃₄	42	29	1	1	4	2	4	1	0	0	2	0	0	1	0	1	1	1	0	2	0	0	1	0	0	0	0	0	1	0	0	0
Ms₃₅	42	61	1	1	1	0	2	1	0	0	0	1	0	3	0	0	0	3	0	0	0	0	0	1	0	1	0	0	2	0	1	0
Ms₃₆	33	64	0	1	3	1	4	0	0	0	2	0	0	2	1	0	0	2	0	1	0	0	1	0	1	0	1	2	0	0	0	0
Ms₃₇	44	67	0	1	5	1	0	2	1	0	1	0	1	1	0	0	2	1	0	1	0	0	1	0	0	0	0	0	0	0	0	0
Ms₃₈	46	31	0	0	2	3	2	0	0	0	2	0	2	0	1	0	0	0	0	4	0	2	0	0	0	1	0	0	0	1	0	0
Ms₃₉	42	26	1	1	4	2	3	1	0	0	2	0	0	3	0	1	1	3	0	2	0	0	1	0	0	0	0	0	1	0	0	0
Ms₄₀	29	43	1	1	7	2	2	1	1	0	3	0	0	2	0	0	0	2	0	1	0	0	0	1	0	0	0	1	1	0	0	0
Ms₄₁	40	59	0	1	3	2	0	0	0	0	3	0	0	2	1	0	0	2	0	1	0	0	1	0	1	1	0	2	0	0	0	0
Ms₄₂	44	61	0	1	4	1	0	1	1	0	1	0	1	1	0	0	2	1	0	1	0	0	1	1	0	0	0	0	0	0	0	0
Ms₄₃	44	65	0	1	4	1	0	2	1	0	1	0	1	1	0	0	2	1	0	1	0	0	1	0	0	0	0	0	0	0	0	0
Ms₄₄	48	23	0	1	3	2	1	0	0	0	1	0	2	1	1	0	0	1	0	4	0	1	0	0	0	0	0	0	0	1	0	0