干旱和UV-B辐射胁迫及其互作对白沙蒿抗性生理的影响

doi:10.11686/cyxb2020290

草业学报 ›› 2021, Vol. 30 ›› Issue (8): 109-118.DOI: 10.11686/cyxb2020290

• 研究论文 • 上一篇

干旱和UV-B辐射胁迫及其互作对白沙蒿抗性生理的影响

韩晓栩¹(), 赵媛媛¹(), 张丽静¹(), 郭丁¹, 傅华¹, 李永善², 杨成新³

^1.兰州大学草地农业生态系统国家重点实验室，兰州大学农业农村部草牧业创新重点实验室，兰州大学草地农业科技学院，甘肃兰州 730020
^2.内蒙古自治区孪井滩气象站，内蒙古嘉尔嘎勒赛汉镇 750312
^3.内蒙古自治区阿拉善盟草原工作站，内蒙古巴彦浩特 750306

收稿日期:2020-06-24 修回日期:2020-09-03 出版日期:2021-07-09 发布日期:2021-07-09
通讯作者: 张丽静
作者简介:Corresponding author. E-mail： lijingzhang@lzu.edu.cn
韩晓栩（1995-），男，黑龙江鹤岗人，在读硕士。E-mail： 794191289@qq.com
赵媛媛（1991-），女，山东威海人，在读博士。E-mail： 18893147262@163.com。第一联系人：共同第一作者 These authors contributed equally to this work.
基金资助:
国家自然科学基金(31770763);新疆维吾尔自治区重大科技专项项目(2016A03006);国家重点基础研究发展计划（973计划）(2014CB138703)

Interactive effects of drought and UV-B radiation on physiological defences in Artemisia sphaerocephala

Xiao-xu HAN¹(), Yuan-yuan ZHAO¹(), Li-jing ZHANG¹(), Ding GUO¹, Hua FU¹, Yong-shan LI², Cheng-xin YANG³

^1.State Key Laboratory of Grassland Agro-ecosystems，Key Laboratory of Grassland Livestock Industry Innovation，Ministry of Agriculture and Rural Affairs，College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，China
^2.Luanjingtan Meteorological Station，Inner Mongolia，Gargaresehan 750312，China
^3.Alxa Alliance Grassland Station，Inner Mongolia，Bayan Hot 750306，China

Received:2020-06-24 Revised:2020-09-03 Online:2021-07-09 Published:2021-07-09
Contact: Li-jing ZHANG

摘要/Abstract

摘要：

以白沙蒿为试验材料，在干旱（D）、UV-B辐射（U）和干旱与UV-B辐射复合（D+U）胁迫下，从幼苗生长、膜脂氧化、次生物质类黄酮、脂肪酸代谢及其基因表达等方面研究了干旱和UV-B辐射胁迫及其互作对白沙蒿抗性生理的影响。结果显示，D和U处理下，白沙蒿幼苗叶、茎、根生物量及总生物量积累减少，株高、叶面积和相对含水量（RWC）降低。D+U处理缓解了D和U处理造成的白沙蒿生物量的下降。D和U处理下，叶相对电导率（REC）显著升高；D+U处理的REC显著下降。D处理的丙二醛（MDA）含量和脂氧合酶（LOX）活性分别为对照（CK）的1.65和3.69倍，而U处理MDA含量和LOX活性无显著变化；D+U处理MDA含量和LOX活性分别为D处理的66.69%和44.00%。D和U处理类黄酮含量分别为CK的1.25和1.37倍；D+U处理类黄酮含量为D处理的1.57倍。D处理未引起不饱和脂肪酸指数（IUFA）显著变化，U处理造成IUFA显著降低，为CK的91.96%；D+U处理IUFA为U处理的1.08倍。结果表明，干旱和UV-B辐射胁迫引起的膜损伤是造成白沙蒿生物量下降的主要原因；干旱和UV-B辐射复合胁迫通过增加类黄酮含量、抑制LOX活性和提高脂肪酸不饱和度的效应产生叠加作用，缓解了彼此对白沙蒿造成的膜损伤。

关键词: 白沙蒿, 干旱, UV-B辐射, 类黄酮, 脂肪酸

Abstract:

This research explored the physiological effects of drought （D）， UV-B radiation stress （U） and their interactions （D+U） on Artemisia sphaerocephala and its stress tolerance. The seedling growth， membrane lipid oxidation， flavonoid metabolism， fatty acid metabolism and FAD gene expression in A. sphaerocephala were studied under D， U， and D+U， and unstressed （CK） treatments. In D and UV treatments， leaf weight， stem weight， root weight， total biomass， plant height， leaf area and relative water content were decreased， compared to CK. For the D+UV treatment， biomass reductions were less than those observed in D or UV treatments applied alone. Compared to CK， relative electric conductivity increased significantly in both D and UV treatments， but decreased significantly in the D+UV treatment. Malondialdehyde （MDA） content and lipoxygenase （LOX） activity in the D treatment were， respectively， 1.65 and 3.69 times those in the control treatment （CK）， while MDA content and LOX activity in the UV treatment showed no significant change. MDA content and LOX activity in the D+UV treatment were， respectively， 66.69% and 44.00% of those in the D treatment. Flavonoid content in D and UV treatments was， respectively， 1.25 and 1.37 times that in CK. Flavonoid content in the D+UV treatment was 1.57 times that in the D treatment. An index of unsaturated fatty acid （IUFA） in the D treatment showed no significant change， while IUFA in the UV treatment were 91.96% of that in CK. IUFA in the D+UV treatment was 1.08 times of that in UV treatment. These results indicate： An important reason for the stress-induced biomass decline in A. sphaerocephala was that drought and UV-B radiation caused membrane damage； The combined stress of drought and UV-B radiation inhibited LOX activity by increasing flavonoid content and increased unsaturated fatty acids， which alleviated the membrane damage caused by drought and UV-B radiation in A. sphaerocephala.

Key words: Artemisia sphaerocephala, drought, UV-B radiation, flavonoid, fatty acid

韩晓栩, 赵媛媛, 张丽静, 郭丁, 傅华, 李永善, 杨成新. 干旱和UV-B辐射胁迫及其互作对白沙蒿抗性生理的影响[J]. 草业学报, 2021, 30(8): 109-118.

Xiao-xu HAN, Yuan-yuan ZHAO, Li-jing ZHANG, Ding GUO, Hua FU, Yong-shan LI, Cheng-xin YANG. Interactive effects of drought and UV-B radiation on physiological defences in Artemisia sphaerocephala[J]. Acta Prataculturae Sinica, 2021, 30(8): 109-118.

图/表 7

表1 干旱和UV-B辐射及其互作对白沙蒿幼苗生长的影响

Table 1 Effect of growth indices on A. sphaerocephala seedlings treated with drought and UV-B radiation acting individually and in combination

处理 Treatment	生物量Biomass （g·plant^-1）				根冠比 Root-shoot ratio	株高 Plant height （cm）	叶面积 Leaf area （cm²）
处理 Treatment	叶Leaf	茎Stem	根Root	合计Total	根冠比 Root-shoot ratio	株高 Plant height （cm）	叶面积 Leaf area （cm²）
CK	0.57±0.02a	0.85±0.05a	1.13±0.08a	2.56±0.11a	0.79±0.04a	38.70±0.17a	1.45±0.01a
D	0.43±0.00c	0.60±0.01b	0.68±0.09c	1.70±0.10c	0.66±0.08a	30.27±1.20c	0.74±0.02d
U	0.43±0.01c	0.75±0.02a	0.83±0.07bc	2.01±0.09b	0.71±0.05a	31.63±1.16c	1.08±0.01c
D+U	0.51±0.00b	0.81±0.03a	0.93±0.05ab	2.25±0.03b	0.71±0.05a	34.60±0.15b	1.25±0.02b

图1 干旱和UV-B辐射及其互作对白沙蒿叶相对含水量的影响不同小写字母表示在P<0.05水平上差异显著。下同。Different lowercase letters mean significant difference at P<0.05. The same below.

Fig.1 Effect of RWC on A. sphaerocephala leaves treated with drought and UV-B radiation acting individually and in combination

图2 干旱和UV-B辐射及其互作对白沙蒿叶相对电导率、丙二醛含量和脂氧合酶活性的影响

Fig.2 Effect of REC， MDA content and LOX activity on A. sphaerocephala leaves treated with drought and UV-B radiation acting individually and in combination

图3 干旱和UV-B辐射及其互作对白沙蒿叶类黄酮含量及其合成途径中关键酶的影响

Fig.3 Effect of flavonoids content and key enzymes in its synthesis pathway on A. sphaerocephala leaves treated with drought and UV-B radiation acting individually and in combination

表2 干旱和UV-B辐射及其互作对白沙蒿叶脂肪酸组成及含量的影响

Table 2 Effect of fatty acid composition and content on A. sphaerocephala leaves treated with drought and UV-B radiation acting individually and in combination （%）

脂肪酸Fatty acid	CK	D	U	D+U
C16：0	19.37±0.12a	17.26±0.05c	18.44±0.08b	19.13±0.10a
C18：0	2.30±0.15a	2.15±0.24a	2.61±0.41a	2.33±0.25a
C18：1	3.17±0.01c	3.76±0.11b	8.78±0.10a	3.01±0.03c
C18：2	29.40±0.06b	23.80±0.10d	32.24±0.28a	28.31±0.21c
C18：3	42.19±0.27b	45.03±0.34a	33.38±0.29c	42.36±0.28b
C20：0	3.57±0.36b	7.99±0.19a	4.55±0.44b	4.87±0.08b
SFA	25.24±0.33b	27.40±0.37a	25.61±0.46b	26.32±0.24ab
UFA	74.76±0.33a	72.60±0.37b	74.39±0.46a	73.68±0.24ab
IUFA	188.54±0.92a	188.45±1.01a	173.40±1.31b	186.71±0.72a

图4 干旱和UV-B辐射及其互作对白沙蒿叶中FAD基因表达的影响

Fig.4 Effect of FAD gene expression on A. sphaerocephala leaves treated with drought and UV-B radiation acting individually and in combination

图5 干旱和UV-B辐射及其互作对白沙蒿类黄酮和脂肪酸代谢的影响方框内的“↑”和“↓”分别代表胁迫对白沙蒿的促进和抑制作用。 ↑ and ↓ in the box respectively represent the promotion and inhibition of stress in A. sphaerocephala.

Fig.5 Effects of drought and UV-B radiation on flavonoid metabolism and fatty acid metabolism in A. sphaerocephala

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干旱和UV-B辐射胁迫及其互作对白沙蒿抗性生理的影响

Interactive effects of drought and UV-B radiation on physiological defences in Artemisia sphaerocephala

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