草业学报 ›› 2022, Vol. 31 ›› Issue (6): 69-78.DOI: 10.11686/cyxb2021179
孙晓梵1(), 张一龙1, 李培英1,2,3(
), 孙宗玖1,2,3
收稿日期:
2021-05-07
修回日期:
2021-06-09
出版日期:
2022-06-20
发布日期:
2022-05-11
通讯作者:
李培英
作者简介:
E-mail: 823797457@qq.com基金资助:
Xiao-fan SUN1(), Yi-long ZHANG1, Pei-ying LI1,2,3(
), Zong-jiu SUN1,2,3
Received:
2021-05-07
Revised:
2021-06-09
Online:
2022-06-20
Published:
2022-05-11
Contact:
Pei-ying LI
摘要:
为探讨干旱下施氮肥是否可以提高狗牙根抗旱性,以新农1号狗牙根为材料,干旱前进行不施氮(0 mmol·L-1)、低氮(1.5 mmol·L-1)、中氮(7.5 mmol·L-1)、高氮(15.0 mmol·L-1) 4种施氮处理,盆栽试验控制水分缓慢干旱,在干旱0、7、14 d、复水7 d,测定草坪质量、叶片相对含水量、相对电导率、丙二醛含量、抗氧化酶活性[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)]、过氧化氢以及渗透调节物质可溶性蛋白、脯氨酸、可溶性糖含量,并对干旱7、14 d下狗牙根抗旱性响应指标进行热图聚类及主成分分析。结果表明,不同施氮量对狗牙根草坪质量的影响因干旱程度不同而有所不同。正常灌溉和中度干旱下高氮处理(15 mmol·L-1)下狗牙根表现出较好的草坪质量,而中氮处理(7.5 mmol·L-1) 能显著提高其重度干旱下草坪质量。与干旱对照相比,干旱处理下施中氮可使狗牙根叶片相对含水量、CAT活性、SOD活性、可溶性蛋白、脯氨酸和可溶性糖含量依次显著提高60.98%、154.40%、163.73%、61.75%、144.71%和31.53%,维持较高的草坪质量。且复水后中氮和高氮处理狗牙根草坪质量恢复较快,可能是由于在干旱胁迫时受到损伤较小。综上认为,干旱前施中氮,可以一定程度缓解干旱胁迫对狗牙根幼苗造成的伤害。
孙晓梵, 张一龙, 李培英, 孙宗玖. 不同施氮量对干旱下狗牙根抗氧化酶活性及渗透调节物质含量的影响[J]. 草业学报, 2022, 31(6): 69-78.
Xiao-fan SUN, Yi-long ZHANG, Pei-ying LI, Zong-jiu SUN. Effects of different nitrogen application rates on antioxidant activity and content of substances involved in osmotic adjustment in Cynodon dactylon under drought stress[J]. Acta Prataculturae Sinica, 2022, 31(6): 69-78.
图1 干旱及复水对土壤含水量的影响不同小写字母表示处理间差异显著(P<0.05),大写字母CK、D、DL、DM、DH分别表示正常灌溉+无氮、干旱+无氮、低氮+干旱、中氮+干旱、高氮+干旱,数字0、7、14、R-7分别代表第0、7、14天及复水7天后的处理天数,下同。Different lowercase letters indicate significant difference between treatments (P<0.05). CK, D, DL, DM, DH indicate normal irrigation+No-n, drought+No-n, low-n+drought, medium-n+drought, high-n+drought respectively, the numbers 0, 7, 14 and R-7 represent the treatment days after day 0, 7, 14 and the 7th day after re-watering respectively. The same below.
Fig.1 Effects of drought and re-watering on soil water content
图2 干旱及复水下不同施氮量对狗牙根幼苗草坪质量和叶片相对含水量的影响
Fig.2 Effects of different nitrogen rates on turf quality and leaf relative water content of bermudagrass seedlings under drought and re-watering
图3 干旱及复水下不同施氮量对狗牙根幼苗相对电导率及丙二醛含量的影响
Fig.3 Effects of different nitrogen rates on relative electric conductivity and MDA content of bermudagrass seedlings under drought and re-watering
图4 干旱及复水下不同施氮量对狗牙根幼苗H2O2含量及抗氧化酶活性的影响
Fig.4 Effects of different nitrogen rates on H2O2 content and antioxidant enzyme activities of bermudagrass seedlings under drought and re-watering
图5 干旱胁迫及复水下不同施氮量对狗牙根幼苗可溶性蛋白、脯氨酸和可溶性糖含量的影响
Fig.5 Effects of different nitrogen rates on soluble protein, proline and soluble sugar content of bermudagrass seedlings under drought and re-watering
图6 干旱下不同施氮量对狗牙根幼苗各指标热图聚类分析以及主成分分析
Fig.6 Heat map cluster analysis and principal component analysis of different nitrogen application rates on the indexes of bermudagrass seedlings under drought
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