草业学报 ›› 2023, Vol. 32 ›› Issue (12): 171-180.DOI: 10.11686/cyxb2023068
孟晨1,2,3(), 鲁雪莉1,2,3, 王菊英2, 魏云冲4, 张成省1,2,3, 李义强1,2,3, 徐宗昌1,2,3()
收稿日期:
2023-03-06
修回日期:
2023-04-21
出版日期:
2023-12-20
发布日期:
2023-10-18
通讯作者:
徐宗昌
作者简介:
Corresponding author. E-mail: xuzongchang@caas.cn基金资助:
Chen MENG1,2,3(), Xue-li LU1,2,3, Ju-ying WANG2, Yun-chong WEI4, Cheng-sheng ZHANG1,2,3, Yi-qiang LI1,2,3, Zong-chang XU1,2,3()
Received:
2023-03-06
Revised:
2023-04-21
Online:
2023-12-20
Published:
2023-10-18
Contact:
Zong-chang XU
摘要:
土壤盐渍化是制约农业生产的重要因素,小黑麦具有高产抗逆等特性,具有改良盐碱地的作用。以小黑麦为材料,以5种盐(NaCl, KCl, Na2SO4, MgSO4, NaHCO3),并设置10、25、50、100以及200 mmol·L-1盐浓度梯度以及混合盐(NaHCO3∶NaCl∶Na2SO4=1∶15∶84),设置0.05%,0.15%,0.30%,0.60%和1.20%盐浓度梯度对小黑麦进行盐胁迫处理,分析盐胁迫下小黑麦种子萌发阶段的发芽率、发芽势、发芽指数、活力指数及幼苗生长阶段的株高和根长,通过主成分分析及隶属函数分析对不同盐胁迫下小黑麦耐盐性进行综合评价。结果表明,小黑麦种子随着盐处理浓度的升高,其发芽率、发芽势、发芽指数和活力指数均逐渐降低,高浓度盐处理显著影响小黑麦种子的萌发。小黑麦幼苗株高及根长也随着盐处理浓度的增加而呈逐渐降低的趋势,其中NaHCO3的抑制作用最显著。主成分分析表明,评价小黑麦耐盐性的主要指标为发芽率、发芽指数及根长。此外,隶属函数值排序表明,小黑麦对混合盐处理的耐受度相对较高,对NaHCO3的耐受度最低。
孟晨, 鲁雪莉, 王菊英, 魏云冲, 张成省, 李义强, 徐宗昌. 不同类型盐胁迫对小黑麦种子萌发的影响[J]. 草业学报, 2023, 32(12): 171-180.
Chen MENG, Xue-li LU, Ju-ying WANG, Yun-chong WEI, Cheng-sheng ZHANG, Yi-qiang LI, Zong-chang XU. Effects of different salt stresses on triticale seed germination[J]. Acta Prataculturae Sinica, 2023, 32(12): 171-180.
指标 Index | 处理 Treatment | NaCl | KCl | Na2SO4 | MgSO4 | NaHCO3 | 混合盐 Salts mixture |
---|---|---|---|---|---|---|---|
发芽势 Germination energy | CK | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a |
1 | 90.00±10.00a | 86.67±6.67a | 93.33±3.33a | 95.00±5.00a | 80.00±1.36b | 91.67±1.67ab | |
2 | 93.33±1.36a | 95.00±5.00a | 76.67±0.00c | 88.33±1.67ab | 80.00±3.33b | 88.33±1.67ab | |
3 | 90.00±0.00a | 93.33±6.67a | 85.00±1.67b | 85.00±1.67b | 66.67±3.33bc | 88.33±1.67ab | |
4 | 88.33±1.67a | 86.67±6.67a | 83.33±0.00b | 81.67±1.67b | 46.67±3.33d | 86.67±0.00b | |
5 | 3.33±3.33b | 5.00±5.00b | 0.00±0.00d | 51.67±8.33c | 3.33±3.33e | 68.33±8.33c | |
发芽率 Germination rate | CK | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a |
1 | 95.56±5.09a | 92.22±1.92a | 90.00±0.00b | 93.33±3.33ab | 83.33±0.00b | 93.33±1.36ab | |
2 | 93.33±1.36ab | 90.00±3.33a | 80.00±3.33c | 88.89±1.92bc | 80.00±3.33b | 88.89±1.92bc | |
3 | 88.89±1.92b | 93.33±6.67a | 85.56±1.92b | 85.56±1.92c | 72.22±5.09c | 90.00±3.33bc | |
4 | 90.00±3.33b | 92.22±1.92a | 66.67±3.33d | 68.89±1.92d | 20.00±6.67d | 86.67±0.00c | |
5 | 0.00±0.00c | 26.67±26.67b | 0.00±0.00e | 15.56±1.92e | 0.00±0.00e | 53.33±3.33d | |
相对盐害率 Relative salt injury rate | 1 | 0.00(1) | 3.49(3) | 5.81(4) | 2.33(2) | 12.79(8) | 2.33(2) |
2 | 2.33(2) | 5.81(4) | 16.28(9) | 6.98(5) | 16.28(9) | 6.98(5) | |
3 | 6.98(5) | 2.33(2) | 10.47(7) | 10.47(7) | 24.42(10) | 5.81(4) | |
4 | 5.81(4) | 3.49(3) | 30.23(12) | 27.91(11) | 79.07(15) | 9.30(6) | |
5 | 100.00(17) | 72.09(14) | 100.00(17) | 83.72(16) | 100.00(17) | 44.19(13) |
表1 不同类型盐胁迫对小黑麦种子萌发指标的影响
Table 1 Effect of different salt stresses on germination indicators of triticale seeds (%)
指标 Index | 处理 Treatment | NaCl | KCl | Na2SO4 | MgSO4 | NaHCO3 | 混合盐 Salts mixture |
---|---|---|---|---|---|---|---|
发芽势 Germination energy | CK | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a | 95.00±1.67a |
1 | 90.00±10.00a | 86.67±6.67a | 93.33±3.33a | 95.00±5.00a | 80.00±1.36b | 91.67±1.67ab | |
2 | 93.33±1.36a | 95.00±5.00a | 76.67±0.00c | 88.33±1.67ab | 80.00±3.33b | 88.33±1.67ab | |
3 | 90.00±0.00a | 93.33±6.67a | 85.00±1.67b | 85.00±1.67b | 66.67±3.33bc | 88.33±1.67ab | |
4 | 88.33±1.67a | 86.67±6.67a | 83.33±0.00b | 81.67±1.67b | 46.67±3.33d | 86.67±0.00b | |
5 | 3.33±3.33b | 5.00±5.00b | 0.00±0.00d | 51.67±8.33c | 3.33±3.33e | 68.33±8.33c | |
发芽率 Germination rate | CK | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a | 95.56±1.92a |
1 | 95.56±5.09a | 92.22±1.92a | 90.00±0.00b | 93.33±3.33ab | 83.33±0.00b | 93.33±1.36ab | |
2 | 93.33±1.36ab | 90.00±3.33a | 80.00±3.33c | 88.89±1.92bc | 80.00±3.33b | 88.89±1.92bc | |
3 | 88.89±1.92b | 93.33±6.67a | 85.56±1.92b | 85.56±1.92c | 72.22±5.09c | 90.00±3.33bc | |
4 | 90.00±3.33b | 92.22±1.92a | 66.67±3.33d | 68.89±1.92d | 20.00±6.67d | 86.67±0.00c | |
5 | 0.00±0.00c | 26.67±26.67b | 0.00±0.00e | 15.56±1.92e | 0.00±0.00e | 53.33±3.33d | |
相对盐害率 Relative salt injury rate | 1 | 0.00(1) | 3.49(3) | 5.81(4) | 2.33(2) | 12.79(8) | 2.33(2) |
2 | 2.33(2) | 5.81(4) | 16.28(9) | 6.98(5) | 16.28(9) | 6.98(5) | |
3 | 6.98(5) | 2.33(2) | 10.47(7) | 10.47(7) | 24.42(10) | 5.81(4) | |
4 | 5.81(4) | 3.49(3) | 30.23(12) | 27.91(11) | 79.07(15) | 9.30(6) | |
5 | 100.00(17) | 72.09(14) | 100.00(17) | 83.72(16) | 100.00(17) | 44.19(13) |
项目 Item | 处理 Treatment | NaCl | KCl | Na2SO4 | MgSO4 | NaHCO3 | 混合盐 Salts mixture |
---|---|---|---|---|---|---|---|
发芽指数 Germination index | CK | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a |
1 | 12.98±1.19a | 13.05±0.45a | 13.42±0.08a | 13.58±1.08a | 11.53±0.33b | 12.98±0.11a | |
2 | 13.25±0.33a | 13.29±0.71a | 11.20±0.37c | 13.17±0.33ab | 11.74±0.29b | 12.04±0.46b | |
3 | 13.06±0.22a | 13.29±0.96a | 11.92±0.25b | 12.22±0.64bc | 8.13±0.43c | 11.81±0.03b | |
4 | 12.62±0.67a | 12.16±0.59a | 9.26±0.44d | 11.29±0.29c | 5.10±0.57d | 10.08±0.33c | |
5 | 1.52±0.43b | 2.02±1.62b | 0.17±0.17e | 5.08±0.18d | 0.39±0.19e | 6.25±0.73d | |
活力指数 Vigor index | CK | 10.89±0.11ab | 10.89±0.11a | 10.89±0.11a | 10.89±0.11a | 10.89±0.11a | 10.89±0.11a |
1 | 11.55±0.19a | 11.23±0.57a | 10.05±0.69b | 10.26±0.72a | 9.07±0.43b | 10.06±0.46a | |
2 | 11.73±0.44a | 10.67±0.31a | 8.08±0.52c | 9.37±0.39b | 7.99±1.40b | 9.19±0.38ab | |
3 | 10.47±0.17b | 10.28±0.96a | 7.99±0.42c | 6.00±0.39c | 6.31±0.45c | 10.83±0.40a | |
4 | 7.85±0.92c | 8.01±0.29b | 2.15±0.28d | 2.68±0.48d | 0.39±0.15d | 6.84±2.48b | |
5 | 0.00±0.00d | 0.86±1.03c | 0.00±0.00e | 0.22±0.02e | 0.00±0.00d | 4.13±1.75c |
表2 不同类型盐胁迫对小黑麦种子发芽指数和活力指数的影响
Table 2 Effect of different salt treatment on seed germination index and vigor index
项目 Item | 处理 Treatment | NaCl | KCl | Na2SO4 | MgSO4 | NaHCO3 | 混合盐 Salts mixture |
---|---|---|---|---|---|---|---|
发芽指数 Germination index | CK | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a | 13.38±0.12a |
1 | 12.98±1.19a | 13.05±0.45a | 13.42±0.08a | 13.58±1.08a | 11.53±0.33b | 12.98±0.11a | |
2 | 13.25±0.33a | 13.29±0.71a | 11.20±0.37c | 13.17±0.33ab | 11.74±0.29b | 12.04±0.46b | |
3 | 13.06±0.22a | 13.29±0.96a | 11.92±0.25b | 12.22±0.64bc | 8.13±0.43c | 11.81±0.03b | |
4 | 12.62±0.67a | 12.16±0.59a | 9.26±0.44d | 11.29±0.29c | 5.10±0.57d | 10.08±0.33c | |
5 | 1.52±0.43b | 2.02±1.62b | 0.17±0.17e | 5.08±0.18d | 0.39±0.19e | 6.25±0.73d | |
活力指数 Vigor index | CK | 10.89±0.11ab | 10.89±0.11a | 10.89±0.11a | 10.89±0.11a | 10.89±0.11a | 10.89±0.11a |
1 | 11.55±0.19a | 11.23±0.57a | 10.05±0.69b | 10.26±0.72a | 9.07±0.43b | 10.06±0.46a | |
2 | 11.73±0.44a | 10.67±0.31a | 8.08±0.52c | 9.37±0.39b | 7.99±1.40b | 9.19±0.38ab | |
3 | 10.47±0.17b | 10.28±0.96a | 7.99±0.42c | 6.00±0.39c | 6.31±0.45c | 10.83±0.40a | |
4 | 7.85±0.92c | 8.01±0.29b | 2.15±0.28d | 2.68±0.48d | 0.39±0.15d | 6.84±2.48b | |
5 | 0.00±0.00d | 0.86±1.03c | 0.00±0.00e | 0.22±0.02e | 0.00±0.00d | 4.13±1.75c |
图2 盐胁迫下小黑麦的株高、根长及须根数目不同小写字母表示差异显著(P<0.05)。Different lowercase letters indicate significant differences at 0.05 level.
Fig.2 Seedling length, root length and fiber root number of triticale under salt stress
指标 Index | NaCl | KCl | Na2SO4 | MgSO4 | NaHCO3 | 混合盐Salts mixture | |
---|---|---|---|---|---|---|---|
F1 | F2 | ||||||
发芽势GE | 0.992 | 0.981 | 0.952 | 0.976 | 0.960 | 0.963 | -0.165 |
发芽率GR | 0.993 | 0.984 | 0.991 | 0.980 | 0.997 | 0.963 | -0.193 |
株高SL | 0.978 | 0.979 | 0.943 | 0.959 | 0.993 | 0.874 | 0.370 |
根长RL | 0.884 | 0.582 | 0.807 | 0.775 | 0.897 | 0.810 | 0.564 |
须根数目RN | 0.975 | 0.847 | 0.968 | 0.986 | 0.973 | -0.327 | 0.900 |
发芽指数GI | 0.991 | 0.989 | 0.992 | 0.970 | 0.978 | 0.988 | -0.105 |
简易活力指数SVI | 0.984 | 0.988 | 0.993 | 0.958 | 0.990 | 0.981 | 0.154 |
特征值Eigen value | 7.595 | 6.863 | 7.201 | 7.223 | 7.581 | 6.252 | 1.402 |
贡献率Contribution (%) | 94.931 | 85.787 | 90.013 | 90.290 | 94.765 | 78.149 | 17.521 |
累计贡献率Cumulative contribution (%) | 94.931 | 85.787 | 90.013 | 90.290 | 94.765 | 78.149 | 95.670 |
表3 耐盐性指标的主成分分析
Table 3 Principal component analysis of salt tolerance evaluation indexes
指标 Index | NaCl | KCl | Na2SO4 | MgSO4 | NaHCO3 | 混合盐Salts mixture | |
---|---|---|---|---|---|---|---|
F1 | F2 | ||||||
发芽势GE | 0.992 | 0.981 | 0.952 | 0.976 | 0.960 | 0.963 | -0.165 |
发芽率GR | 0.993 | 0.984 | 0.991 | 0.980 | 0.997 | 0.963 | -0.193 |
株高SL | 0.978 | 0.979 | 0.943 | 0.959 | 0.993 | 0.874 | 0.370 |
根长RL | 0.884 | 0.582 | 0.807 | 0.775 | 0.897 | 0.810 | 0.564 |
须根数目RN | 0.975 | 0.847 | 0.968 | 0.986 | 0.973 | -0.327 | 0.900 |
发芽指数GI | 0.991 | 0.989 | 0.992 | 0.970 | 0.978 | 0.988 | -0.105 |
简易活力指数SVI | 0.984 | 0.988 | 0.993 | 0.958 | 0.990 | 0.981 | 0.154 |
特征值Eigen value | 7.595 | 6.863 | 7.201 | 7.223 | 7.581 | 6.252 | 1.402 |
贡献率Contribution (%) | 94.931 | 85.787 | 90.013 | 90.290 | 94.765 | 78.149 | 17.521 |
累计贡献率Cumulative contribution (%) | 94.931 | 85.787 | 90.013 | 90.290 | 94.765 | 78.149 | 95.670 |
盐处理 Salt treatment | 发芽势 GE | 发芽率 GR | 株高 SL | 根长 RL | 须根数目 RN | 发芽指数 GI | 简易活力指数 SVI | 平均值 Average | 排序 Rank |
---|---|---|---|---|---|---|---|---|---|
CK | 1.0000 | 1.0000 | 0.9065 | 0.9502 | 0.9490 | 0.9845 | 0.9279 | 0.9597 | 1 |
25 mmol·L-1 NaCl | 0.9825 | 0.9767 | 1.0000 | 0.8571 | 0.9082 | 0.9752 | 1.0000 | 0.9571 | 2 |
10 mmol·L-1 NaCl | 0.9474 | 1.0000 | 0.9632 | 0.8773 | 0.9082 | 0.9547 | 0.9847 | 0.9479 | 3 |
0.30%混合盐Salts mixture | 0.9298 | 0.9419 | 0.9577 | 1.0000 | 0.9490 | 0.8677 | 0.9235 | 0.9385 | 4 |
10 mmol·L-1 KCl | 0.9123 | 0.9651 | 0.9687 | 0.5007 | 1.0000 | 0.9602 | 0.9575 | 0.8949 | 5 |
0.05%混合盐Salts mixture | 0.9649 | 0.9767 | 0.8573 | 0.8254 | 0.7959 | 0.9547 | 0.8573 | 0.8903 | 6 |
50 mmol·L-1 NaCl | 0.9474 | 0.9302 | 0.9373 | 0.5664 | 0.8776 | 0.9609 | 0.8924 | 0.8732 | 7 |
25 mmol·L-1 KCl | 1.0000 | 0.9419 | 0.9438 | 0.3947 | 0.9388 | 0.9783 | 0.9099 | 0.8725 | 8 |
10 mmol·L-1 MgSO4 | 1.0000 | 0.9767 | 0.8737 | 0.3968 | 0.9592 | 1.0000 | 0.8744 | 0.8687 | 9 |
10 mmol·L-1 Na2SO4 | 0.9825 | 0.9419 | 0.8881 | 0.4704 | 0.9490 | 0.9876 | 0.8564 | 0.8680 | 10 |
50 mmol·L-1 KCl | 0.9825 | 0.9767 | 0.8757 | 0.4141 | 0.8776 | 0.9783 | 0.8766 | 0.8545 | 11 |
25 mmol·L-1 MgSO4 | 0.9298 | 0.9302 | 0.8384 | 0.3586 | 0.9184 | 0.9689 | 0.7987 | 0.8204 | 12 |
0.15%混合盐Salts mixture | 0.9298 | 0.9302 | 0.8220 | 0.5859 | 0.7857 | 0.8851 | 0.7830 | 0.8174 | 13 |
10 mmol·L-1 NaHCO3 | 0.8421 | 0.8721 | 0.8662 | 0.4798 | 0.9388 | 0.8472 | 0.7734 | 0.8028 | 14 |
100 mmol·L-1 NaCl | 0.9298 | 0.9419 | 0.6922 | 0.5245 | 0.9184 | 0.9280 | 0.6688 | 0.8005 | 15 |
50 mmol·L-1 Na2SO4 | 0.8947 | 0.8953 | 0.7429 | 0.4380 | 0.9592 | 0.8758 | 0.6813 | 0.7839 | 16 |
100 mmol·L-1 KCl | 0.9123 | 0.9651 | 0.6917 | 0.3442 | 0.9286 | 0.8938 | 0.6830 | 0.7741 | 17 |
25 mmol·L-1 Na2SO4 | 0.8070 | 0.8372 | 0.8026 | 0.4026 | 0.9184 | 0.8224 | 0.6884 | 0.7541 | 18 |
25 mmol·L-1 NaHCO3 | 0.8421 | 0.8372 | 0.7916 | 0.3377 | 0.8980 | 0.8627 | 0.6811 | 0.7501 | 19 |
0.60%混合盐Salts mixture | 0.9123 | 0.9070 | 0.6280 | 0.5325 | 0.8571 | 0.7391 | 0.5832 | 0.7370 | 20 |
50 mmol·L-1 MgSO4 | 0.8947 | 0.8953 | 0.5584 | 0.2049 | 0.8367 | 0.8988 | 0.5113 | 0.6857 | 21 |
50 mmol·L-1 NaHCO3 | 0.7018 | 0.7558 | 0.6947 | 0.3752 | 0.7653 | 0.5938 | 0.5376 | 0.6320 | 22 |
1.20%混合盐Salts mixture | 0.7193 | 0.5581 | 0.6072 | 0.4697 | 0.9694 | 0.4534 | 0.3523 | 0.5899 | 23 |
100 mmol·L-1 Na2SO4 | 0.8772 | 0.6977 | 0.2586 | 0.2698 | 0.8469 | 0.6776 | 0.1836 | 0.5445 | 24 |
100 mmol·L-1 MgSO4 | 0.8596 | 0.7209 | 0.3093 | 0.0970 | 0.4898 | 0.8292 | 0.2289 | 0.5050 | 25 |
200 mmol·L-1 KCl | 0.0526 | 0.2791 | 0.1721 | 0.2504 | 0.8163 | 0.1379 | 0.0732 | 0.2545 | 26 |
100 mmol·L-1 NaHCO3 | 0.4912 | 0.2093 | 0.1522 | 0.0000 | 0.0000 | 0.3677 | 0.0331 | 0.1791 | 27 |
200 mmol·L-1 MgSO4 | 0.5439 | 0.1628 | 0.1139 | 0.0000 | 0.0000 | 0.3658 | 0.0189 | 0.1722 | 28 |
200 mmol·L-1 NaCl | 0.0351 | 0.0000 | 0.0796 | 0.2049 | 0.6735 | 0.1007 | 0.0000 | 0.1562 | 29 |
200 mmol·L-1 Na2SO4 | 0.0000 | 0.0000 | 0.0271 | 0.0970 | 0.4245 | 0.0000 | 0.0000 | 0.0784 | 30 |
200 mmol·L-1 NaHCO3 | 0.0351 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0168 | 0.0000 | 0.0074 | 31 |
表4 隶属函数值及耐盐性综合评价
Table 4 Subjection values and comprehensive evaluation of salt tolerance of triticale under different salt treatments
盐处理 Salt treatment | 发芽势 GE | 发芽率 GR | 株高 SL | 根长 RL | 须根数目 RN | 发芽指数 GI | 简易活力指数 SVI | 平均值 Average | 排序 Rank |
---|---|---|---|---|---|---|---|---|---|
CK | 1.0000 | 1.0000 | 0.9065 | 0.9502 | 0.9490 | 0.9845 | 0.9279 | 0.9597 | 1 |
25 mmol·L-1 NaCl | 0.9825 | 0.9767 | 1.0000 | 0.8571 | 0.9082 | 0.9752 | 1.0000 | 0.9571 | 2 |
10 mmol·L-1 NaCl | 0.9474 | 1.0000 | 0.9632 | 0.8773 | 0.9082 | 0.9547 | 0.9847 | 0.9479 | 3 |
0.30%混合盐Salts mixture | 0.9298 | 0.9419 | 0.9577 | 1.0000 | 0.9490 | 0.8677 | 0.9235 | 0.9385 | 4 |
10 mmol·L-1 KCl | 0.9123 | 0.9651 | 0.9687 | 0.5007 | 1.0000 | 0.9602 | 0.9575 | 0.8949 | 5 |
0.05%混合盐Salts mixture | 0.9649 | 0.9767 | 0.8573 | 0.8254 | 0.7959 | 0.9547 | 0.8573 | 0.8903 | 6 |
50 mmol·L-1 NaCl | 0.9474 | 0.9302 | 0.9373 | 0.5664 | 0.8776 | 0.9609 | 0.8924 | 0.8732 | 7 |
25 mmol·L-1 KCl | 1.0000 | 0.9419 | 0.9438 | 0.3947 | 0.9388 | 0.9783 | 0.9099 | 0.8725 | 8 |
10 mmol·L-1 MgSO4 | 1.0000 | 0.9767 | 0.8737 | 0.3968 | 0.9592 | 1.0000 | 0.8744 | 0.8687 | 9 |
10 mmol·L-1 Na2SO4 | 0.9825 | 0.9419 | 0.8881 | 0.4704 | 0.9490 | 0.9876 | 0.8564 | 0.8680 | 10 |
50 mmol·L-1 KCl | 0.9825 | 0.9767 | 0.8757 | 0.4141 | 0.8776 | 0.9783 | 0.8766 | 0.8545 | 11 |
25 mmol·L-1 MgSO4 | 0.9298 | 0.9302 | 0.8384 | 0.3586 | 0.9184 | 0.9689 | 0.7987 | 0.8204 | 12 |
0.15%混合盐Salts mixture | 0.9298 | 0.9302 | 0.8220 | 0.5859 | 0.7857 | 0.8851 | 0.7830 | 0.8174 | 13 |
10 mmol·L-1 NaHCO3 | 0.8421 | 0.8721 | 0.8662 | 0.4798 | 0.9388 | 0.8472 | 0.7734 | 0.8028 | 14 |
100 mmol·L-1 NaCl | 0.9298 | 0.9419 | 0.6922 | 0.5245 | 0.9184 | 0.9280 | 0.6688 | 0.8005 | 15 |
50 mmol·L-1 Na2SO4 | 0.8947 | 0.8953 | 0.7429 | 0.4380 | 0.9592 | 0.8758 | 0.6813 | 0.7839 | 16 |
100 mmol·L-1 KCl | 0.9123 | 0.9651 | 0.6917 | 0.3442 | 0.9286 | 0.8938 | 0.6830 | 0.7741 | 17 |
25 mmol·L-1 Na2SO4 | 0.8070 | 0.8372 | 0.8026 | 0.4026 | 0.9184 | 0.8224 | 0.6884 | 0.7541 | 18 |
25 mmol·L-1 NaHCO3 | 0.8421 | 0.8372 | 0.7916 | 0.3377 | 0.8980 | 0.8627 | 0.6811 | 0.7501 | 19 |
0.60%混合盐Salts mixture | 0.9123 | 0.9070 | 0.6280 | 0.5325 | 0.8571 | 0.7391 | 0.5832 | 0.7370 | 20 |
50 mmol·L-1 MgSO4 | 0.8947 | 0.8953 | 0.5584 | 0.2049 | 0.8367 | 0.8988 | 0.5113 | 0.6857 | 21 |
50 mmol·L-1 NaHCO3 | 0.7018 | 0.7558 | 0.6947 | 0.3752 | 0.7653 | 0.5938 | 0.5376 | 0.6320 | 22 |
1.20%混合盐Salts mixture | 0.7193 | 0.5581 | 0.6072 | 0.4697 | 0.9694 | 0.4534 | 0.3523 | 0.5899 | 23 |
100 mmol·L-1 Na2SO4 | 0.8772 | 0.6977 | 0.2586 | 0.2698 | 0.8469 | 0.6776 | 0.1836 | 0.5445 | 24 |
100 mmol·L-1 MgSO4 | 0.8596 | 0.7209 | 0.3093 | 0.0970 | 0.4898 | 0.8292 | 0.2289 | 0.5050 | 25 |
200 mmol·L-1 KCl | 0.0526 | 0.2791 | 0.1721 | 0.2504 | 0.8163 | 0.1379 | 0.0732 | 0.2545 | 26 |
100 mmol·L-1 NaHCO3 | 0.4912 | 0.2093 | 0.1522 | 0.0000 | 0.0000 | 0.3677 | 0.0331 | 0.1791 | 27 |
200 mmol·L-1 MgSO4 | 0.5439 | 0.1628 | 0.1139 | 0.0000 | 0.0000 | 0.3658 | 0.0189 | 0.1722 | 28 |
200 mmol·L-1 NaCl | 0.0351 | 0.0000 | 0.0796 | 0.2049 | 0.6735 | 0.1007 | 0.0000 | 0.1562 | 29 |
200 mmol·L-1 Na2SO4 | 0.0000 | 0.0000 | 0.0271 | 0.0970 | 0.4245 | 0.0000 | 0.0000 | 0.0784 | 30 |
200 mmol·L-1 NaHCO3 | 0.0351 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0168 | 0.0000 | 0.0074 | 31 |
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