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草业学报 ›› 2021, Vol. 30 ›› Issue (9): 97-104.DOI: 10.11686/cyxb2020338

• 研究论文 • 上一篇    下一篇

GSH对铅胁迫下多年生黑麦草生长及光合生理的影响

赵利清1,2(), 彭向永3, 刘俊祥1, 毛金梅4, 孙振元1()   

  1. 1.国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京 100091
    2.国家开放大学,北京 100039
    3.曲阜师范大学生命科学学院,山东 曲阜 273165
    4.新疆林业科学院经济林研究所,新疆 乌鲁木齐 830063
  • 收稿日期:2020-07-15 修回日期:2020-11-10 出版日期:2021-08-30 发布日期:2021-08-30
  • 通讯作者: 孙振元
  • 作者简介:Corresponding author. E-mail: sunzy@263.net
    赵利清(1976-),女,内蒙古呼和浩特人,副教授,在读博士。E-mail: 195432830@qq.com
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项资金(CAFYBB2018ZB002)

Effects of reduced glutathione on the growth and photosynthesis of perennial ryegrass under lead stress

Li-qing ZHAO1,2(), Xiang-yong PENG3, Jun-xiang LIU1, Jin-mei MAO4, Zhen-yuan SUN1()   

  1. 1.Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China
    2.The Open University of China,Beijing 100039,China
    3.College of Life Science Qufu Normal University,Qufu 273165,China
    4.Economic Forest Research Institute,Xinjiang Academy of Forestry,Urumqi 830063,China
  • Received:2020-07-15 Revised:2020-11-10 Online:2021-08-30 Published:2021-08-30
  • Contact: Zhen-yuan SUN

摘要:

为了明确谷胱甘肽(GSH)对多年生黑麦草铅(Pb)毒害的缓解作用及其生理机制,以12周龄多年生黑麦草‘卡特’幼苗为试验材料,设置4个试验处理:1)根部置1/2 Hoagland营养液,叶面喷蒸馏水50 mL(CK);2)根部置含0.75 mmol·L-1 Pb(NO32的1/2 Hoagland营养液,叶面喷蒸馏水50 mL(Pb);3)根部置含0.75 mmol·L-1 Pb(NO32的1/2 Hoagland营养液,叶面先喷25 mL蒸馏水,待吸收后再喷施25 mL 10 mmol·L-1 GSH(Pb+GSH);4)根部置含0.75 mmol·L-1 Pb(NO32的1/2 Hoagland营养液,叶面先喷25 mL蒸馏水,待吸收后再喷施25 mL 1 mmol·L-1丁硫氨酸-亚砜亚胺(BSO)(Pb+BSO),研究GSH对多年生黑麦草生长及光合作用的影响。结果表明:叶面喷施GSH显著增加了幼苗茎叶长、根长、分蘖数、生物量、叶绿素含量和光合参数,也增加了叶绿素荧光动力学参数。叶面喷施BSO后,根长、分蘖数、生物量、类胡萝卜素含量和光合参数均降低。综上所述,Pb胁迫影响了光合作用,最终抑制了植物的生长。叶面喷施10 mmol·L-1 GSH能够缓解Pb对多年生黑麦草生长和光合作用的胁迫,提高植物的抗性。相反,叶面喷施1 mmol·L-1 BSO能够加剧Pb对植物的胁迫。

关键词: 多年生黑麦草, 谷胱甘肽, 生长, 光合作用,

Abstract:

The aim of this study was to explore the mitigative effects of reduced glutathione (GSH) on the growth and photosynthesis of perennial ryegrass (Lolium perenne) under lead stress. Twelve-week old seedlings of the perennial ryegrass cultivar ‘cuttle’ were used as the experimental material. In the control (CK), leaves were sprayed with 50 mL distilled water, and roots were supplied with 1/2-strength modified Hoagland’s nutrient solution. In the other three experimental treatments, the roots were supplied with 1/2-strength modified Hoagland’s nutrient solution containing 0.75 mmol·L-1 Pb(NO32, and the leaves were sprayed with 50 mL distilled water (Pb treatment); with 25 mL distilled water followed by 25 mL 10 mmol·L-1 GSH (Pb+GSH treatment); or with 25 mL distilled water followed by 25 mL 1 mmol·L-1 L-buthionine-sulfoximine (BSO) (Pb+BSO treatment). The results showed that shoot length, root length, tiller number, biomass, chlorophyll content, photosynthetic parameters, and the kinetic parameters of chlorophyll fluorescence were significantly higher in the Pb+GSH treatment than in Pb treatment. In contrast, the values for root length, tiller number, biomass, carotenoid content, and photosynthetic parameters were lower in the Pb+BSO treatment than in Pb treatment. Together, our results show that spraying ryegrass leaves with 10 mmol·L-1 GSH can effectively alleviate the inhibitory effects of Pb stress and improve the Pb tolerance of perennial ryegrass, whereas spraying with BSO at 1 mmol·L-1 exacerbates the inhibitory effects of Pb stress.

Key words: perennial ryegrass, glutathione, growth, photosynthesis, lead