Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (8): 199-205.DOI: 10.11686/cyxb2020283
Xiang GUO1(), De-kui CHEN2, Na CHEN2, Yun LI3, Xiao-yang CHEN1(), Qing ZHANG1()
Received:
2020-06-23
Revised:
2020-09-24
Online:
2021-07-09
Published:
2021-07-09
Contact:
Xiao-yang CHEN,Qing ZHANG
Xiang GUO, De-kui CHEN, Na CHEN, Yun LI, Xiao-yang CHEN, Qing ZHANG. Effect of moisture content and additives on the fermentation quality of Neolamarckia cadamba leaf silage[J]. Acta Prataculturae Sinica, 2021, 30(8): 199-205.
项目Item | 含量Content |
---|---|
干物质DM (%) | 27.69 |
粗蛋白CP (%DM) | 12.48 |
水溶性碳水化合物WSC (%DM) | 4.49 |
中性洗涤纤维NDF (%DM) | 30.84 |
酸性洗涤纤维ADF (%DM) | 21.66 |
乳酸菌LAB (log10 cfu·g -1 FM) | 4.48 |
酵母菌Yeasts (log10 cfu·g-1 FM) | 4.19 |
霉菌Moulds (log10 cfu·g-1 FM) | ND |
大肠杆菌CB (log10 cfu·g-1 FM) | 5.59 |
总酚Total phenol (%DM) | 5.62 |
简单酚SP (%DM) | 1.15 |
水解单宁HT (%DM) | 4.47 |
缩合单宁CT (%DM) | 1.72 |
Table 1 The characteristic of the pre-ensiled N. cadamba leaves
项目Item | 含量Content |
---|---|
干物质DM (%) | 27.69 |
粗蛋白CP (%DM) | 12.48 |
水溶性碳水化合物WSC (%DM) | 4.49 |
中性洗涤纤维NDF (%DM) | 30.84 |
酸性洗涤纤维ADF (%DM) | 21.66 |
乳酸菌LAB (log10 cfu·g -1 FM) | 4.48 |
酵母菌Yeasts (log10 cfu·g-1 FM) | 4.19 |
霉菌Moulds (log10 cfu·g-1 FM) | ND |
大肠杆菌CB (log10 cfu·g-1 FM) | 5.59 |
总酚Total phenol (%DM) | 5.62 |
简单酚SP (%DM) | 1.15 |
水解单宁HT (%DM) | 4.47 |
缩合单宁CT (%DM) | 1.72 |
项目 Item | 含水量 Moisture | 添加剂Additives | 标准误 SEM | 差异显著性Significance | |||||
---|---|---|---|---|---|---|---|---|---|
CK | LP | C | LP+C | W | A | W×A | |||
干物质DM (%) | W1 | 23.59b | 23.76b | 22.15b | 23.17b | 0.52 | ** | NS | NS |
W2 | 38.99a | 32.66a | 36.08a | 31.75a | 1.53 | ||||
酸碱度pH | W1 | 3.86bA | 3.74bBC | 3.80bB | 3.72bC | 0.02 | ** | ** | NS |
W2 | 4.07aA | 3.99aB | 4.01aA | 3.97aB | 0.01 | ||||
乳酸菌LAB (log10 cfu·g-1 FM) | W1 | 5.09bA | <2.00bC | 4.57bAB | 3.20bB | 0.69 | ** | ** | NS |
W2 | 5.18a | 4.33a | 5.36a | 5.07a | 0.16 | ||||
酵母菌Yeasts (log10 cfu·g-1 FM) | W1 | 1.80bB | <2.00bB | 3.70A | 2.46bB | 1.13 | ** | ** | NS |
W2 | 3.60aA | 3.06aB | 3.85A | 4.03aA | 0.50 | ||||
大肠杆菌CB (log10 cfu·g-1 FM) | W1 | <2.00 | <2.00 | <2.00 | <2.00 | 0 | NS | NS | NS |
W2 | <2.00 | <2.00 | <2.00 | <2.00 | 0 | ||||
乳酸LA (%DM) | W1 | 4.39a | 4.43 | 4.50a | 4.63 | 0.25 | ** | ** | ** |
W2 | 2.44bB | 4.53A | 3.09bB | 5.03A | 0.25 | ||||
乙酸AA (%DM) | W1 | 0.080a | 0.060a | 0.020a | 0.050a | 0.01 | ** | NS | * |
W2 | 0.006b | 0.007b | 0.014b | 0.008b | 0.01 | ||||
丙酸PA (%DM) | W1 | ND | ND | ND | ND | 0 | NS | NS | NS |
W2 | ND | ND | ND | ND | 0 | ||||
NDF (%DM) | W1 | 29.99bA | 30.31bA | 25.81bB | 26.62B | 0.50 | * | ** | NS |
W2 | 32.19aA | 32.41aA | 29.43aAB | 27.79B | 1.90 | ||||
ADF (%DM) | W1 | 24.06A | 22.94A | 19.72B | 20.53B | 0.55 | NS | ** | NS |
W2 | 24.90A | 25.29A | 23.09AB | 21.48B | 1.58 |
Table 2 Effect of moisture and additives on fermentation characteristics and microbial populations of N. cadamba leaves silage after 60 days ensiling
项目 Item | 含水量 Moisture | 添加剂Additives | 标准误 SEM | 差异显著性Significance | |||||
---|---|---|---|---|---|---|---|---|---|
CK | LP | C | LP+C | W | A | W×A | |||
干物质DM (%) | W1 | 23.59b | 23.76b | 22.15b | 23.17b | 0.52 | ** | NS | NS |
W2 | 38.99a | 32.66a | 36.08a | 31.75a | 1.53 | ||||
酸碱度pH | W1 | 3.86bA | 3.74bBC | 3.80bB | 3.72bC | 0.02 | ** | ** | NS |
W2 | 4.07aA | 3.99aB | 4.01aA | 3.97aB | 0.01 | ||||
乳酸菌LAB (log10 cfu·g-1 FM) | W1 | 5.09bA | <2.00bC | 4.57bAB | 3.20bB | 0.69 | ** | ** | NS |
W2 | 5.18a | 4.33a | 5.36a | 5.07a | 0.16 | ||||
酵母菌Yeasts (log10 cfu·g-1 FM) | W1 | 1.80bB | <2.00bB | 3.70A | 2.46bB | 1.13 | ** | ** | NS |
W2 | 3.60aA | 3.06aB | 3.85A | 4.03aA | 0.50 | ||||
大肠杆菌CB (log10 cfu·g-1 FM) | W1 | <2.00 | <2.00 | <2.00 | <2.00 | 0 | NS | NS | NS |
W2 | <2.00 | <2.00 | <2.00 | <2.00 | 0 | ||||
乳酸LA (%DM) | W1 | 4.39a | 4.43 | 4.50a | 4.63 | 0.25 | ** | ** | ** |
W2 | 2.44bB | 4.53A | 3.09bB | 5.03A | 0.25 | ||||
乙酸AA (%DM) | W1 | 0.080a | 0.060a | 0.020a | 0.050a | 0.01 | ** | NS | * |
W2 | 0.006b | 0.007b | 0.014b | 0.008b | 0.01 | ||||
丙酸PA (%DM) | W1 | ND | ND | ND | ND | 0 | NS | NS | NS |
W2 | ND | ND | ND | ND | 0 | ||||
NDF (%DM) | W1 | 29.99bA | 30.31bA | 25.81bB | 26.62B | 0.50 | * | ** | NS |
W2 | 32.19aA | 32.41aA | 29.43aAB | 27.79B | 1.90 | ||||
ADF (%DM) | W1 | 24.06A | 22.94A | 19.72B | 20.53B | 0.55 | NS | ** | NS |
W2 | 24.90A | 25.29A | 23.09AB | 21.48B | 1.58 |
项目 Item | 含水量 Moisture | 添加剂Additives | 标准误 SEM | 差异显著性Significance | |||||
---|---|---|---|---|---|---|---|---|---|
CK | LP | C | LP+C | W | A | W×A | |||
总酚 Total phenols (%DM) | W1 | 1.33b | 2.23 | 1.80b | 1.32b | 0.16 | ** | NS | NS |
W2 | 2.50a | 2.23 | 2.29a | 2.26a | 0.60 | ||||
简单酚 Simple phenols (%DM) | W1 | 0.15b | 0.27 | 0.19b | 0.14b | 0.03 | ** | NS | NS |
W2 | 0.28a | 0.25 | 0.27a | 0.25a | 0.02 | ||||
水解单宁 Hydrolysable tannin (%DM) | W1 | 1.18b | 2.00 | 1.61 | 1.18b | 0.15 | ** | NS | NS |
W2 | 2.23a | 1.98 | 2.02 | 2.01a | 0.23 | ||||
缩合单宁 Condensed tannin (%DM) | W1 | 1.01A | 0.72B | 0.98bA | 0.57bB | 0.04 | ** | * | * |
W2 | 0.94 | 0.94 | 1.20a | 1.07a | 0.07 |
Table 3 Effect of moisture and additives on tannin contents of N. cadamba leaves silage after 60 days ensiling
项目 Item | 含水量 Moisture | 添加剂Additives | 标准误 SEM | 差异显著性Significance | |||||
---|---|---|---|---|---|---|---|---|---|
CK | LP | C | LP+C | W | A | W×A | |||
总酚 Total phenols (%DM) | W1 | 1.33b | 2.23 | 1.80b | 1.32b | 0.16 | ** | NS | NS |
W2 | 2.50a | 2.23 | 2.29a | 2.26a | 0.60 | ||||
简单酚 Simple phenols (%DM) | W1 | 0.15b | 0.27 | 0.19b | 0.14b | 0.03 | ** | NS | NS |
W2 | 0.28a | 0.25 | 0.27a | 0.25a | 0.02 | ||||
水解单宁 Hydrolysable tannin (%DM) | W1 | 1.18b | 2.00 | 1.61 | 1.18b | 0.15 | ** | NS | NS |
W2 | 2.23a | 1.98 | 2.02 | 2.01a | 0.23 | ||||
缩合单宁 Condensed tannin (%DM) | W1 | 1.01A | 0.72B | 0.98bA | 0.57bB | 0.04 | ** | * | * |
W2 | 0.94 | 0.94 | 1.20a | 1.07a | 0.07 |
项目 Item | 含水量 Moisture | 添加剂Additives | 标准误 SEM | 差异显著性Significance | |||||
---|---|---|---|---|---|---|---|---|---|
CK | LP | C | LP+C | W | A | W×A | |||
粗蛋白 Crude protein (%DM) | W1 | 12.51B | 12.79B | 13.32A | 13.57A | 0.17 | NS | ** | NS |
W2 | 13.07 | 12.27 | 13.47 | 12.90 | 0.17 | ||||
真蛋白 True protein (%DM) | W1 | 10.75 | 11.37 | 11.64 | 11.86 | 0.33 | NS | * | NS |
W2 | 11.26 | 10.54 | 11.82 | 11.18 | 0.29 | ||||
非蛋白氮 Non-protein nitrogen (%DM) | W1 | 1.70 | 1.42 | 1.69 | 1.71 | 0.31 | NS | NS | NS |
W2 | 1.81 | 1.73 | 1.65 | 1.72 | 0.16 | ||||
游离氨基酸 Free amino acid (%DM) | W1 | 1.09 | 1.19 | 1.47 | 1.34 | 0.06 | NS | * | * |
W2 | 1.40 | 1.30 | 1.40 | 1.38 | 0.05 | ||||
氨态氮 Ammonia nitrogen (%DM) | W1 | 0.010 | 0.008b | 0.009b | 0.008 | 0.002 | ** | NS | NS |
W2 | 0.015 | 0.021a | 0.018a | 0.011 | 0.005 |
Table 4 Effect of moisture and additives on nitrogen fractions of N. cadamba leaves silage after 60 days ensiling
项目 Item | 含水量 Moisture | 添加剂Additives | 标准误 SEM | 差异显著性Significance | |||||
---|---|---|---|---|---|---|---|---|---|
CK | LP | C | LP+C | W | A | W×A | |||
粗蛋白 Crude protein (%DM) | W1 | 12.51B | 12.79B | 13.32A | 13.57A | 0.17 | NS | ** | NS |
W2 | 13.07 | 12.27 | 13.47 | 12.90 | 0.17 | ||||
真蛋白 True protein (%DM) | W1 | 10.75 | 11.37 | 11.64 | 11.86 | 0.33 | NS | * | NS |
W2 | 11.26 | 10.54 | 11.82 | 11.18 | 0.29 | ||||
非蛋白氮 Non-protein nitrogen (%DM) | W1 | 1.70 | 1.42 | 1.69 | 1.71 | 0.31 | NS | NS | NS |
W2 | 1.81 | 1.73 | 1.65 | 1.72 | 0.16 | ||||
游离氨基酸 Free amino acid (%DM) | W1 | 1.09 | 1.19 | 1.47 | 1.34 | 0.06 | NS | * | * |
W2 | 1.40 | 1.30 | 1.40 | 1.38 | 0.05 | ||||
氨态氮 Ammonia nitrogen (%DM) | W1 | 0.010 | 0.008b | 0.009b | 0.008 | 0.002 | ** | NS | NS |
W2 | 0.015 | 0.021a | 0.018a | 0.011 | 0.005 |
1 | Dehghani M R, Weisbjerg M R, Hvelplund T, et al. Effect of enzyme addition to forage at ensiling on silage chemical composition and NDF degradation characteristics. Livestock Science, 2012, 150(1/2/3): 51-58. |
2 | Wang Y, Wang C, Zhou W, et al. Effects of wilting and Lactobacillus plantarum addition on the fermentation quality and microbial community of Moringa oleifera leaf silage. Frontiers in Microbiology, 2018, 9: 1817 |
3 | Zhang Q, Yu Z, Yang H, et al. The effects of stage of growth and additives with or without cellulase on fermentation and in vitro degradation characteristics of Leymus chinensis silage. Grass & Forage Science, 2016, 71(4): 595-606. |
4 | Wang C, Wang Y, Zhou W, et al. Effects of Lactobacillus plantarum (LP) and moisture on feed quality and tannin content of Moringa oleifera leaf silage. Acta Prataculturae Sinica, 2019, 28(6): 109-118. |
王成, 王益, 周玮, 等. 植物乳杆菌和含水量对辣木叶青贮品质和单宁含量的影响. 草业学报, 2019, 28(6): 109-118. | |
5 | Shu S M, Yang C H, Tang Z S, et al. Effects of different moisture contents with previously fermented juice on the quality of whipgrass (Hemarthria compressa) silage. Grass Feeding Livestock, 2011(4): 41-43. |
舒思敏, 杨春华, 唐智松, 等. 添加绿汁发酵液对不同含水量扁穗牛鞭草青贮料品质的影响. 草食家畜, 2011(4): 41-43. | |
6 | Xu Q F, Yu Z, Han J G, et al. Determining organic acid in alfalfa silage by HPLC. Grassland and Turf, 2007(2): 63-65. |
许庆方, 玉柱, 韩建国, 等. 高效液相色谱法测定紫花苜蓿青贮中的有机酸. 草原与草坪, 2007(2): 63-65. | |
7 | Zhang Q, Li X J, Zhao M M, et al. Isolating and evaluating lactic acid bacteria strains for effectiveness of Leymus chinensis silage fermentation. Letters in Applied Microbiology, 2014, 59: 391-397. |
8 | Zhang L Y. Feed analysis and quality detection technology (Third Edition). Beijing: China Agricultural University Press, 2007: 49-75. |
张丽英. 饲料分析及质量检测技术(第3版). 北京: 中国农业大学出版社, 2007: 49-75. | |
9 | Broderick G A, Kang J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 1980, 63(1): 64-75. |
10 | Licitra G, Hernandez T M, Van Soest P J. Standardization of procedures for nitrogen. Animal Feed Science Technology, 1996, 57(4): 347-358. |
11 | Guo L H, Yang S K. Study on gallic acid preparation by using immobilized tannase from Aspergillus niger. Chinese Journal of Biotechnology, 2000, 16(5): 614-617. |
郭鲁宏, 杨顺楷. 利用固定化黑曲霉单宁酶制备没食子酸的研究. 生物工程学报, 2000, 16(5): 614-617. | |
12 | Harinder P S M. Quantification of tannins in tree and shrub foliage. Dordrecht: Springer, 1900. |
13 | Coblentz W K, Grabber J H. In situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration. Journal of Dairy Science, 2013, 96: 3120-3137. |
14 | Yitbarek M B, Tamir B. Silage additives: Review. Open Journal of Applied Sciences, 2014, 4(5): 258-274. |
15 | Li Y, Nishino N. Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri. Letters in Applied Microbiology, 2015, 52(4): 314-321. |
16 | Sun Z H, Liu S M, Tayo G O, et al. Effects of cellulase or lactic acid bacteria on silage fermentation and in vitro gas production of several morphological fractions of maize stover. Animal Feed Science & Technology, 2009, 152(3): 219-231. |
17 | Hathway D E. Chapter 5: The condensed tannins. Egham, Surrey, England. Wood Extractives & Their Significance to the Pulp & Paper Industries, 1962, 20(6): 191-228. |
18 | Huo Z H, Fang R J. Investigation of anti-nutrition mechanism of tannin to ruminant and its removing measures. Chinese Feed, 2007, 20: 20-23. |
霍振华, 方热军. 单宁对反刍动物的抗营养作用机理及其消除措施. 中国饲料, 2007, 20: 20-23. | |
19 | Jana A, Maity C, Halder S K, et al. Structural characterization of thermostable, solvent tolerant, cytosafe tannase from Bacillus subtilis PAB2. Biochemical Engineering Journal, 2013, 77: 161-170. |
20 | Dong W C, Lin Y F, Zhu H F, et al. Research progress of regulation of plant tannins on lactic acid bacteria characteristics and its application in production. Chinese Journal of Animal Nutrition, 2019, 31(5): 2063-2068. |
董文成, 林语梵, 朱鸿福, 等. 植物单宁调控乳酸菌特性的研究进展及其在生产中的应用. 动物营养学报, 2019, 31(5): 2063-2068. | |
21 | Wan J M, Sun X Y, Etuk E B, et al. Antinutritional factors in sorghum: Chemistry, mode of action and effects on livestock poultry. Animal Husbandry Abroad (Pigs and Poultry), 2015, 35(2): 36-38. |
万建美, 孙相俞, Etuk E B, 等. 高粱中抗营养因子的化学性质及其对畜禽的影响与作用机制. 国外畜牧学(猪与禽), 2015, 35(2): 36-38. | |
22 | Mutabaruka R, Hairiah K, Cadisch G. Microbial degradation of hydrolysable and condensed tannin polyphenol-protein complexes in soils from different land-use histories. Soil Biology & Biochemistry, 2007, 39(7): 1479-1492. |
23 | Jones B A, Muck R E, Hatfleld R D. Red clover extracts inhibit legume proteolysis. Journal of the Science of Food and Agriculture, 1995, 67(3): 329-333. |
24 | Pang J M, Han D D, Tan S Z, et al. Nutritional value evaluation of Moringa oleifera Lam. in growing pigs. Chinese Journal of Animal Nutrition, 2021, 33(1): 199-207. |
庞家满, 韩丹丹, 谈苏贞, 等. 辣木在生长猪上的营养价值评定. 动物营养学报, 2021, 33(1): 199-207. |
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