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草业学报 ›› 2026, Vol. 35 ›› Issue (2): 68-82.DOI: 10.11686/cyxb2025081

• 研究论文 • 上一篇    下一篇

7个紫花苜蓿品种种子萌发及幼苗生长的抗旱性评价

张继元1(), 安海全2, 潘靖一1, 刘畅1, 龙思思1, 赵丽丽1()   

  1. 1.贵州大学动物科学学院草业科学系,贵州 贵阳 550025
    2.安顺市畜牧技术推广站,贵州 安顺 561000
  • 收稿日期:2025-03-11 修回日期:2025-04-29 出版日期:2026-02-20 发布日期:2025-12-24
  • 通讯作者: 赵丽丽
  • 作者简介:Corresponding author. E-mail: zhaolili_0508@163.com
    张继元(2002-),男,贵州六盘水人,在读本科。E-mail: zhangjiyuan010@163.com
  • 基金资助:
    贵州省科技计划项目(黔科合基础-ZK[2023]119,黔科合平台人才[2021]5636 号),国家自然科学基金(32260340);贵州省科技计划项目(黔科合基础-ZK[2023]119,黔科合平台人才[2021]5636 号),国家自然科学基金(32060391);贵州大学大学生科研训练计划项目(贵大SRT字[2022]072)

Drought resistance evaluation of seed germination and seedling growth of seven alfalfa cultivars

Ji-yuan ZHANG1(), Hai-quan AN2, Jing-yi PAN1, Chang LIU1, Si-si LONG1, Li-li ZHAO1()   

  1. 1.Department of Grassland Science,College of Animal Science,Guizhou University,Guiyang 550025,China
    2.Anshun Animal Husbandry Technology Promotion Station,Anshun 561000,China
  • Received:2025-03-11 Revised:2025-04-29 Online:2026-02-20 Published:2025-12-24
  • Contact: Li-li ZHAO

摘要:

以皇冠、角斗士、PANGO、巨能801、WL525、维多利亚、迪特7个紫花苜蓿品种种子为试验材料,采用浓度为15%的聚乙二醇-6000(PEG-6000)溶液模拟干旱胁迫14 d,实验室内分析了种子萌发、幼苗生长和生理生化指标对干旱胁迫的响应与变化。结果表明:干旱胁迫条件下,除PANGO外其余6个紫花苜蓿种子的发芽指数和活力指数均显著下降(P<0.05);皇冠和WL525幼苗的干重、苗长和根长显著增加(P<0.05);巨能801、维多利亚和角斗士幼苗的相对含水量均显著增加(P<0.05);除角斗士外其余6个品种的过氧化物酶(POD)活性均显著上升,除维多利亚外,其余6个品种的丙二醛(MDA)含量均显著上升;皇冠、迪特和巨能801的超氧化物歧化酶(SOD)活性均显著上升;角斗士的脯氨酸(Pro)和可溶性糖(SS)含量显著升高(P<0.05)。基于种子萌发、幼苗生长和生理生化等17个指标,利用主成分分析法结合隶属函数法综合评价,得出7个紫花苜蓿品种种子萌发与幼苗生长期的抗旱性由强到弱的排序为:巨能801>角斗士>WL525>维多利亚>PANGO>迪特>皇冠;基于系统聚类分析划分出:1)强抗旱品种为‘巨能801’;2)中抗旱品种为‘角斗士’、‘WL525’和‘维多利亚’;3)弱抗旱品种为‘PANGO’、‘迪特’和‘皇冠’。

关键词: 紫花苜蓿, 种子萌发, 生理, PEG-6000, 抗旱性评价

Abstract:

In this research a 15% polyethylene glycol-6000 (PEG-6000) solution was employed to simulate drought stress and test germination and seedling growth responses to drought of seven alfalfa (Medicago sativa) cultivars, namely, Phabulous, Gladiator, PANGO, Magnum 801, WL525, Victoria, and Dieter, for 14 days. The responses and changes in seed germination, seedling growth, and physiological and biochemical indexes to drought stress were measured and analyzed in the laboratory. It was found that under drought stress, the germination index and vigor index of the six alfalfa seeds, except PANGO, decreased significantly (P<0.05). The dry weight, seedling length and root length of Phabulous and WL525 seedlings increased significantly (P<0.05). The relative water content of seedlings of Magnum 801, Victoria and Gladiator increased significantly (P<0.05). The peroxidase (POD) activity of the six cultivars, except Gladiator, increased significantly (P<0.05), and the malondialdehyde (MDA) content increased significantly (P<0.05) in all cultivars except Victoria. The superoxide dismutase (SOD) activity increased significantly (P<0.05) in Phabulous, Dieter, and Magnum 801. The proline (Pro) and soluble sugar (SS) content increased significantly (P<0.05) in Gladiator. Using data for 17 traits defining seed germination, seedling growth and physiology and biochemistry, a multivariate evaluation was conducted using principal component analysis combined with the membership function method. In this way, the drought resistance of the seven alfalfa cultivars during seed germination and early seedling growth was ranked from strong to weak as follows: Magnum 801>Gladiator>WL525>Victoria>PANGO>Dieter>Phabulous. According to a hierarchical cluster analysis, the cultivars were classified into three categories: 1) Strongly drought-resistant cultivars are ‘Magnum 801’; 2) Cultivars with medium drought-resistance are ‘Gladiator’, ‘WL525’ and ‘Victoria’; 3) Varieties with weak drought-resistance are ‘PANGO’, ‘Dieter’ and ‘Phabulous’.

Key words: alfalfa, seed germination, physiology, PEG-6000, drought resistance evaluation